Restriction map for
pF1KB7631
(Circular) (Six-base) MAPSORT of: pF1KB7631.seq Check: 7521 from: 1 to: 4073
pF1KB7631 4073 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 140 1111 140
Size: 971 3102
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1898 2330 3077 3345 1898
Size: 432 747 268 2626
Fragments arranged by size:
2626 747 432 268
AfeI AGC'GCT
Cuts at: 508 508
Size: 4073
AflIII A'CryG_T
Cuts at: 701 1425 2529 3506 701
Size: 724 1104 977 1268
Fragments arranged by size:
1268 1104 977 724
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AhdI GACnn_n'nnGTC
Cuts at: 861 861
Size: 4073
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 977 1046 977
Size: 69 4004
AlwNI CAG_nnn'CTG
Cuts at: 594 1378 2945 594
Size: 784 1567 1722
Fragments arranged by size:
1722 1567 784
ApaI G_GGCC'C
Cuts at: 655 655
Size: 4073
ApaLI G'TGCA_C
Cuts at: 313 2843 313
Size: 2530 1543
ApoI r'AATT_y
Cuts at: 1077 3426 3682 1077
Size: 2349 256 1468
Fragments arranged by size:
2349 1468 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 324 663 756 826 1093 324
Size: 339 93 70 267 3304
Fragments arranged by size:
3304 339 267 93 70
BamHI G'GATC_C
Cuts at: 1098 1098
Size: 4073
BanI G'GyrC_C
Cuts at: 365 464 625 763 790 807 903 1089 1503
Size: 99 161 138 27 17 96 186 414
Cuts at: 1503 1751 1786 365
Size: 248 35 2652
Fragments arranged by size:
2652 414 248 186 161 138 99 96
35 27 17
BanII G_rGCy'C
Cuts at: 655 898 1087 2117 655
Size: 243 189 1030 2611
Fragments arranged by size:
2611 1030 243 189
BbeI G_GCGC'C
Cuts at: 468 629 767 794 811 907 1507 1755 468
Size: 161 138 27 17 96 600 248 2786
Fragments arranged by size:
2786 600 248 161 138 96 27 17
BbsI GAAGACnn'nnnn_
Cuts at: 245 245
Size: 4073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 880 914 880
Size: 34 4039
BciVI GTATCCnnnnn_n'
Cuts at: 1971 2732 4073 1971
Size: 761 1341 1971
Fragments arranged by size:
1971 1341 761
BclI T'GATC_A
Cuts at: 1593 1593
Size: 4073
BglI GCCn_nnn'nGGC
Cuts at: 363 403 674 363
Size: 40 271 3762
Fragments arranged by size:
3762 271 40
BglII A'GATC_T
Cuts at: 1588 3625 1588
Size: 2037 2036
BlpI GC'TnA_GC
Cuts at: 1185 1185
Size: 4073
Bme1580I G_kGCm'C
Cuts at: 317 368 655 1030 1698 1791 2847 317
Size: 51 287 375 668 93 1056 1543
Fragments arranged by size:
1543 1056 668 375 287 93 51
BmgBI CAC'GTC
Cuts at: 515 927 515
Size: 412 3661
BmrI ACTGGGnnnn_n'
Cuts at: 996 1285 1366 1701 3845 996
Size: 289 81 335 2144 1224
Fragments arranged by size:
2144 1224 335 289 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 900 2440 900
Size: 1540 2533
Bpu10I CC'TnA_GC
Cuts at: 472 472
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1249 2128 2620 2918 487
Size: 762 879 492 298 1642
Fragments arranged by size:
1642 879 762 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3777 3777
Size: 4073
BsaAI yAC'GTr
Cuts at: 264 334 1426 264
Size: 70 1092 2911
Fragments arranged by size:
2911 1092 70
BsaBI GATnn'nnATC
Cuts at: 6 1587 3624 6
Size: 1581 2037 455
Fragments arranged by size:
2037 1581 455
BsaHI Gr'CG_yC
Cuts at: 465 611 626 764 791 808 904 1013 1504
Size: 146 15 138 27 17 96 109 491
Cuts at: 1504 1752 2454 3950 465
Size: 248 702 1496 588
Fragments arranged by size:
1496 702 588 491 248 146 138 109
96 27 17 15
BsaWI w'CCGG_w
Cuts at: 1008 1262 1783 2461 2735 2882 3293 1008
Size: 254 521 678 274 147 411 1788
Fragments arranged by size:
1788 678 521 411 274 254 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 103
Size: 30 4043
BseYI C'CCAG_C
Cuts at: 358 811 970 1498 2256 2833 3329 358
Size: 453 159 528 758 577 496 1102
Fragments arranged by size:
1102 758 577 528 496 453 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 495
Size: 4073
BsiEI CG_ry'CG
Cuts at: 104 435 1615 1661 2869 104
Size: 331 1180 46 1208 1308
Fragments arranged by size:
1308 1208 1180 331 46
BsiHKAI G_wGCw'C
Cuts at: 293 317 683 1067 1087 1865 2055 2847 293
Size: 24 366 384 20 778 190 792 1519
Fragments arranged by size:
1519 792 778 384 366 190 24 20
Bsp1286I G_dGCh'C
Cuts at: 293 317 368 655 683 898 1030 1067 1087
Size: 24 51 287 28 215 132 37 20
Cuts at: 1087 1698 1791 1865 2055 2117 2847 293
Size: 611 93 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 287 215 190 132 93
74 62 51 37 28 24 20
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 3249 3249
Size: 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 1123 1337 1639 2020 233
Size: 890 214 302 381 2286
Fragments arranged by size:
2286 890 381 302 214
BsrBI CCG'CTC
Cuts at: 50 129 462 761 2365 2419 3903 4068 50
Size: 79 333 299 1604 54 1484 165 55
Fragments arranged by size:
1604 1484 333 299 165 79 55 54
BsrDI GCAATG_nn'
Cuts at: 1985 1985
Size: 4073
BsrFI r'CCGG_y
Cuts at: 2071 2252 2461 2071
Size: 181 209 3683
Fragments arranged by size:
3683 209 181
BssHII G'CGCG_C
Cuts at: 269 351 353 269
Size: 82 2 3989
Fragments arranged by size:
3989 82 2
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 407 594 1450 407
Size: 187 856 3030
Fragments arranged by size:
3030 856 187
BstBI TT'CG_AA
Cuts at: 236 2434 236
Size: 2198 1875
BstXI CCAn_nnnn'nTGG
Cuts at: 873 873
Size: 4073
BstYI r'GATC_y
Cuts at: 1 551 1098 1565 1588 1923 2169 3181 3267
Size: 550 547 467 23 335 246 1012 86
Cuts at: 3267 3279 3625 1
Size: 12 346 449
Fragments arranged by size:
1012 550 547 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 305 669 107
Size: 198 364 3511
Fragments arranged by size:
3511 364 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 1030 1316 1030
Size: 286 3787
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 1658 1832 2223 2250 3595 1658
Size: 174 391 27 1345 2136
Fragments arranged by size:
2136 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 281 826 2096 2306 281
Size: 545 1270 210 2048
Fragments arranged by size:
2048 1270 545 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 789 937 2281 2591 2737 3883 789
Size: 148 1344 310 146 1146 979
Fragments arranged by size:
1344 1146 979 310 148 146
EcoICRI GAG'CTC
Cuts at: 1085 1085
Size: 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1898 2330 3077 3345 1898
Size: 432 747 268 2626
Fragments arranged by size:
2626 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 582 1212 582
Size: 630 3443
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 4073
FspI TGC'GCA
Cuts at: 559 1854 559
Size: 1295 2778
HaeII r_GCGC'y
Cuts at: 468 510 629 767 794 811 907 1507 1755
Size: 42 119 138 27 17 96 600 248
Cuts at: 1755 2777 3756 468
Size: 1022 979 785
Fragments arranged by size:
1022 979 785 600 248 138 119 96
42 27 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 847 879 1915 1945 1947 1977 103
Size: 32 712 32 1036 30 2 30 2199
Fragments arranged by size:
2199 1036 712 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1112 1112
Size: 4073
KasI G'GCGC_C
Cuts at: 464 625 763 790 807 903 1503 1751 464
Size: 161 138 27 17 96 600 248 2786
Fragments arranged by size:
2786 600 248 161 138 96 27 17
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 701 3506 701
Size: 2805 1268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 722 743 752 2744 2928 722
Size: 21 9 1992 184 1867
Fragments arranged by size:
1992 1867 184 21 9
MscI TGG'CCA
Cuts at: 1834 1834
Size: 4073
MslI CAynn'nnrTG
Cuts at: 2062 2189 2062
Size: 127 3946
MspA1I CmG'CkG
Cuts at: 307 362 383 534 657 671 689 749 989
Size: 55 21 151 123 14 18 60 240
Cuts at: 989 1184 1498 1858 2871 3116 307
Size: 195 314 360 1013 245 1264
Fragments arranged by size:
1264 1013 360 314 245 240 195 151
123 60 55 21 18 14
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 465 626 764 791 808 904 1504 1752 465
Size: 161 138 27 17 96 600 248 2786
Fragments arranged by size:
2786 600 248 161 138 96 27 17
NcoI C'CATG_G
Cuts at: 107 107
Size: 4073
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 349 1126 1626 2533 349
Size: 777 500 907 1889
Fragments arranged by size:
1889 907 777 500
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PmlI CAC'GTG
Cuts at: 264 334 264
Size: 70 4003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PspOMI G'GGCC_C
Cuts at: 651 651
Size: 4073
PstI C_TGCA'G
Cuts at: 590 1120 1805 590
Size: 530 685 2858
Fragments arranged by size:
2858 685 530
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 1498 1858 1498
Size: 360 3713
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 1087 1087
Size: 4073
SacII CC_GC'GG
Cuts at: 308 672 308
Size: 364 3709
SalI G'TCGA_C
Cuts at: 1110 1110
Size: 4073
SapI GCTCTTCn'nnn_
Cuts at: 281 2096 2306 281
Size: 1815 210 2048
Fragments arranged by size:
2048 1815 210
SbfI CC_TGCA'GG
Cuts at: 590 1120 590
Size: 530 3543
ScaI AGT'ACT
Cuts at: 3590 3590
Size: 4073
SexAI A'CCwGG_T
Cuts at: 1058 1058
Size: 4073
SfcI C'TryA_G
Cuts at: 33 586 684 744 1116 1801 2794 2985 33
Size: 553 98 60 372 685 993 191 1121
Fragments arranged by size:
1121 993 685 553 372 191 98 60
SfoI GGC'GCC
Cuts at: 466 627 765 792 809 905 1505 1753 466
Size: 161 138 27 17 96 600 248 2786
Fragments arranged by size:
2786 600 248 161 138 96 27 17
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 326 665 828 1095 326
Size: 339 163 267 3304
Fragments arranged by size:
3304 339 267 163
SmlI C'TyrA_G
Cuts at: 502 1228 2143 2635 2897 502
Size: 726 915 492 262 1678
Fragments arranged by size:
1678 915 726 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 1126 1626 1126
Size: 500 3573
StuI AGG'CCT
Cuts at: 490 490
Size: 4073
StyI C'CwwG_G
Cuts at: 107 542 1207 1561 107
Size: 435 665 354 2619
Fragments arranged by size:
2619 665 435 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1015 1656 1789 2248 2457 1015
Size: 641 133 459 209 2631
Fragments arranged by size:
2631 641 459 209 133
TatI w'GTAC_w
Cuts at: 3588 3588
Size: 4073
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4041
Tth111I GACn'n_nGTC
Cuts at: 1870 1870
Size: 4073
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1568 1568
Size: 4073
XmaI C'CCGG_G
Cuts at: 324 663 826 1093 324
Size: 339 163 267 3304
Fragments arranged by size:
3304 339 267 163
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PmlI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI BpmI BseRI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PpuMI
PshAI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI