Restriction map for
pF1KB7632
(Circular) (Six-base) MAPSORT of: pF1KB7632.seq Check: 8036 from: 1 to: 4205
pF1KB7632 4205 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 514 1243 514
Size: 729 3476
Acc65I G'GTAC_C
Cuts at: 1221 1221
Size: 4205
AclI AA'CG_TT
Cuts at: 538 800 4045 538
Size: 262 3245 698
Fragments arranged by size:
3245 698 262
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2462 3209 3477 2030
Size: 432 747 268 2758
Fragments arranged by size:
2758 747 432 268
AflII C'TTAA_G
Cuts at: 393 393
Size: 4205
AflIII A'CryG_T
Cuts at: 1557 2661 3638 1557
Size: 1104 977 2124
Fragments arranged by size:
2124 1104 977
AgeI A'CCGG_T
Cuts at: 2593 2593
Size: 4205
AhdI GACnn_n'nnGTC
Cuts at: 995 995
Size: 4205
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 175 207 175
Size: 32 4173
AlwNI CAG_nnn'CTG
Cuts at: 1510 3077 1510
Size: 1567 2638
ApaI G_GGCC'C
Cuts at: 590 737 1129 1169 590
Size: 147 392 40 3626
Fragments arranged by size:
3626 392 147 40
ApaLI G'TGCA_C
Cuts at: 2975 2975
Size: 4205
ApoI r'AATT_y
Cuts at: 1209 3558 3814 1209
Size: 2349 256 1600
Fragments arranged by size:
2349 1600 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4205
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
AvaI C'yCGr_G
Cuts at: 685 730 1046 1169 1225 685
Size: 45 316 123 56 3665
Fragments arranged by size:
3665 316 123 56 45
BamHI G'GATC_C
Cuts at: 1230 1230
Size: 4205
BanI G'GyrC_C
Cuts at: 129 1221 1635 1883 1918 129
Size: 1092 414 248 35 2416
Fragments arranged by size:
2416 1092 414 248 35
BanII G_rGCy'C
Cuts at: 590 737 1129 1151 1169 1219 2249 590
Size: 147 392 22 18 50 1030 2546
Fragments arranged by size:
2546 1030 392 147 50 22 18
BbeI G_GCGC'C
Cuts at: 1639 1887 1639
Size: 248 3957
BbsI GAAGACnn'nnnn_
Cuts at: 145 145
Size: 4205
BbvCI CC'TCA_GC
Cuts at: 1116 1116
Size: 4205
BciVI GTATCCnnnnn_n'
Cuts at: 2103 2864 4205 2103
Size: 761 1341 2103
Fragments arranged by size:
2103 1341 761
BclI T'GATC_A
Cuts at: 1725 1725
Size: 4205
BglII A'GATC_T
Cuts at: 1720 3757 1720
Size: 2037 2168
BlpI GC'TnA_GC
Cuts at: 521 1317 521
Size: 796 3409
Bme1580I G_kGCm'C
Cuts at: 132 590 730 737 1129 1169 1830 1923 2979
Size: 458 140 7 392 40 661 93 1056
Cuts at: 2979 132
Size: 1358
Fragments arranged by size:
1358 1056 661 458 392 140 93 40
7
BmrI ACTGGGnnnn_n'
Cuts at: 1417 1498 1833 3977 1417
Size: 81 335 2144 1645
Fragments arranged by size:
2144 1645 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 688
Size: 4205
Bpu10I CC'TnA_GC
Cuts at: 1116 1145 1116
Size: 29 4176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 370 1381 2260 2752 3050 370
Size: 1011 879 492 298 1525
Fragments arranged by size:
1525 1011 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3909 3909
Size: 4205
BsaAI yAC'GTr
Cuts at: 895 1558 895
Size: 663 3542
BsaBI GATnn'nnATC
Cuts at: 6 242 1719 3756 6
Size: 236 1477 2037 455
Fragments arranged by size:
2037 1477 455 236
BsaHI Gr'CG_yC
Cuts at: 1636 1884 2586 4082 1636
Size: 248 702 1496 1759
Fragments arranged by size:
1759 1496 702 248
BsaWI w'CCGG_w
Cuts at: 229 1394 1915 2593 2867 3014 3425 229
Size: 1165 521 678 274 147 411 1009
Fragments arranged by size:
1165 1009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 193 839 193
Size: 646 3559
BseYI C'CCAG_C
Cuts at: 118 145 856 1630 2388 2965 3461 118
Size: 27 711 774 758 577 496 862
Fragments arranged by size:
862 774 758 711 577 496 27
BsiEI CG_ry'CG
Cuts at: 104 1747 1793 3001 104
Size: 1643 46 1208 1308
Fragments arranged by size:
1643 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1219 1997 2187 2979 1219
Size: 778 190 792 2445
Fragments arranged by size:
2445 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 132 590 730 737 1129 1151 1169 1219 1830
Size: 458 140 7 392 22 18 50 611
Cuts at: 1830 1923 1997 2187 2249 2979 132
Size: 93 74 190 62 730 1358
Fragments arranged by size:
1358 730 611 458 392 190 140 93
74 62 50 22 18 7
BspEI T'CCGG_A
Cuts at: 229 3425 229
Size: 3196 1009
BspHI T'CATG_A
Cuts at: 3381 3381
Size: 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 696 1255 1469 1771 2152 696
Size: 559 214 302 381 2749
Fragments arranged by size:
2749 559 381 302 214
BsrBI CCG'CTC
Cuts at: 50 600 681 2497 2551 4035 4200 50
Size: 550 81 1816 54 1484 165 55
Fragments arranged by size:
1816 1484 550 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 2117 2117
Size: 4205
BsrFI r'CCGG_y
Cuts at: 152 2203 2384 2593 152
Size: 2051 181 209 1764
Fragments arranged by size:
2051 1764 209 181
BssSI C'ACGA_G
Cuts at: 2476 2834 2476
Size: 358 3847
BstAPI GCAn_nnn'nTGC
Cuts at: 1582 1582
Size: 4205
BstBI TT'CG_AA
Cuts at: 2566 2566
Size: 4205
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4205
BstYI r'GATC_y
Cuts at: 1 1230 1697 1720 2055 2301 3313 3399 3411
Size: 1229 467 23 335 246 1012 86 12
Cuts at: 3411 3757 1
Size: 346 449
Fragments arranged by size:
1229 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1095 1095
Size: 4205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2155 2336 2499 2155
Size: 181 163 3861
Fragments arranged by size:
3861 181 163
BtsI GCAGTG_nn'
Cuts at: 1040 1109 1448 1040
Size: 69 339 3797
Fragments arranged by size:
3797 339 69
DraI TTT'AAA
Cuts at: 1204 1204
Size: 4205
DrdI GACnn_nn'nnGTC
Cuts at: 1911 2769 1911
Size: 858 3347
EaeI y'GGCC_r
Cuts at: 601 1790 1964 2355 2382 3727 601
Size: 1189 174 391 27 1345 1079
Fragments arranged by size:
1345 1189 1079 391 174 27
EagI C'GGCC_G
Cuts at: 1790 1790
Size: 4205
EarI CTCTTCn'nnn_
Cuts at: 329 440 641 788 2228 2438 329
Size: 111 201 147 1440 210 2096
Fragments arranged by size:
2096 1440 210 201 147 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2413 2723 2869 4015 2413
Size: 310 146 1146 2603
Fragments arranged by size:
2603 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1217 1217
Size: 4205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 2030 2462 3209 3477 688
Size: 1342 432 747 268 1416
Fragments arranged by size:
1416 1342 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 586 587 784 1014 1126 1166 1344 586
Size: 1 197 230 112 40 178 3447
Fragments arranged by size:
3447 230 197 178 112 40 1
EcoRI G'AATT_C
Cuts at: 1209 1209
Size: 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 4205
FspI TGC'GCA
Cuts at: 1986 1986
Size: 4205
HaeII r_GCGC'y
Cuts at: 354 1639 1887 2909 3888 354
Size: 1285 248 1022 979 671
Fragments arranged by size:
1285 1022 979 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 469 501 981 1013 2047 2077 2079
Size: 32 139 32 480 32 1034 30 2
Cuts at: 2079 2109 298
Size: 30 2394
Fragments arranged by size:
2394 1034 480 139 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 411 804 873 1244 411
Size: 393 69 371 3372
Fragments arranged by size:
3372 393 371 69
KasI G'GCGC_C
Cuts at: 1635 1883 1635
Size: 248 3957
KpnI G_GTAC'C
Cuts at: 1225 1225
Size: 4205
MluI A'CGCG_T
Cuts at: 3638 3638
Size: 4205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 475 2876 3060 475
Size: 2401 184 1620
Fragments arranged by size:
2401 1620 184
MscI TGG'CCA
Cuts at: 1966 1966
Size: 4205
MslI CAynn'nnrTG
Cuts at: 2194 2321 2194
Size: 127 4078
MspA1I CmG'CkG
Cuts at: 145 520 1316 1630 1990 3003 3248 145
Size: 375 796 314 360 1013 245 1102
Fragments arranged by size:
1102 1013 796 375 360 314 245
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 4205
NarI GG'CG_CC
Cuts at: 1636 1884 1636
Size: 248 3957
NcoI C'CATG_G
Cuts at: 1095 1095
Size: 4205
NdeI CA'TA_TG
Cuts at: 675 675
Size: 4205
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 4205
NspI r_CATG'y
Cuts at: 1258 1758 2665 1258
Size: 500 907 2798
Fragments arranged by size:
2798 907 500
PasI CC'CwG_GG
Cuts at: 1122 1122
Size: 4205
PciI A'CATG_T
Cuts at: 2661 2661
Size: 4205
PflMI CCAn_nnn'nTGG
Cuts at: 772 772
Size: 4205
PfoI T'CCnGG_A
Cuts at: 626 626
Size: 4205
PmeI GTTT'AAAC
Cuts at: 1204 1204
Size: 4205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 922 954 3344 3376 922
Size: 32 2390 32 1751
Fragments arranged by size:
2390 1751 32 32
PpuMI rG'GwC_Cy
Cuts at: 1014 1014
Size: 4205
PspOMI G'GGCC_C
Cuts at: 586 733 1125 1165 586
Size: 147 392 40 3626
Fragments arranged by size:
3626 392 147 40
PspXI vC'TCGA_Gb
Cuts at: 685 1046 685
Size: 361 3844
PstI C_TGCA'G
Cuts at: 1252 1937 1252
Size: 685 3520
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4205
PvuII CAG'CTG
Cuts at: 520 1630 1990 520
Size: 1110 360 2735
Fragments arranged by size:
2735 1110 360
RsrII CG'GwC_CG
Cuts at: 2400 2400
Size: 4205
SacI G_AGCT'C
Cuts at: 1219 1219
Size: 4205
SalI G'TCGA_C
Cuts at: 1242 1242
Size: 4205
SanDI GG'GwC_CC
Cuts at: 1014 1014
Size: 4205
SapI GCTCTTCn'nnn_
Cuts at: 329 641 2228 2438 329
Size: 312 1587 210 2096
Fragments arranged by size:
2096 1587 312 210
SbfI CC_TGCA'GG
Cuts at: 1252 1252
Size: 4205
ScaI AGT'ACT
Cuts at: 3722 3722
Size: 4205
SfcI C'TryA_G
Cuts at: 33 515 1248 1933 2926 3117 33
Size: 482 733 685 993 191 1121
Fragments arranged by size:
1121 993 733 685 482 191
SfoI GGC'GCC
Cuts at: 1637 1885 1637
Size: 248 3957
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4205
SmaI CCC'GGG
Cuts at: 732 1227 732
Size: 495 3710
SmlI C'TyrA_G
Cuts at: 385 393 685 1046 1360 2275 2767 3029 385
Size: 8 292 361 314 915 492 262 1561
Fragments arranged by size:
1561 915 492 361 314 292 262 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4205
SphI G_CATG'C
Cuts at: 1258 1758 1258
Size: 500 3705
StuI AGG'CCT
Cuts at: 370 399 370
Size: 29 4176
StyI C'CwwG_G
Cuts at: 365 788 945 1095 1339 1693 365
Size: 423 157 150 244 354 2877
Fragments arranged by size:
2877 423 354 244 157 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1788 1921 2380 2589 1788
Size: 133 459 209 3404
Fragments arranged by size:
3404 459 209 133
TatI w'GTAC_w
Cuts at: 1082 3720 1082
Size: 2638 1567
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3722 3754 3722
Size: 32 4173
Tth111I GACn'n_nGTC
Cuts at: 2002 2002
Size: 4205
XbaI T'CTAG_A
Cuts at: 1236 1236
Size: 4205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 205 1700 205
Size: 1495 2710
XhoI C'TCGA_G
Cuts at: 685 1046 685
Size: 361 3844
XmaI C'CCGG_G
Cuts at: 730 1225 730
Size: 495 3710
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BmtI BsaXI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI