Restriction map for
pF1KB7636
(Circular) (Six-base) MAPSORT of: pF1KB7636.seq Check: 1795 from: 1 to: 4244
pF1KB7636 4244 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 860 1282 860
Size: 422 3822
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 443 1246 2069 2501 3248 3516 443
Size: 803 823 432 747 268 1171
Fragments arranged by size:
1171 823 803 747 432 268
AflII C'TTAA_G
Cuts at: 970 970
Size: 4244
AflIII A'CryG_T
Cuts at: 746 1596 2700 3677 746
Size: 850 1104 977 1313
Fragments arranged by size:
1313 1104 977 850
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 147 421 1549 3116 147
Size: 274 1128 1567 1275
Fragments arranged by size:
1567 1275 1128 274
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 610 712 1248 3597 3853 610
Size: 102 536 2349 256 1001
Fragments arranged by size:
2349 1001 536 256 102
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 172 1264 172
Size: 1092 3152
AvrII C'CTAG_G
Cuts at: 762 1074 762
Size: 312 3932
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 768 801 768
Size: 33 4211
BamHI G'GATC_C
Cuts at: 921 1269 921
Size: 348 3896
BanI G'GyrC_C
Cuts at: 1260 1674 1922 1957 1260
Size: 414 248 35 3547
Fragments arranged by size:
3547 414 248 35
BanII G_rGCy'C
Cuts at: 1184 1258 2288 1184
Size: 74 1030 3140
Fragments arranged by size:
3140 1030 74
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BbsI GAAGACnn'nnnn_
Cuts at: 671 671
Size: 4244
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 372 1764 372
Size: 1392 2852
BglI GCCn_nnn'nGGC
Cuts at: 362 1039 362
Size: 677 3567
BglII A'GATC_T
Cuts at: 833 1759 3796 833
Size: 926 2037 1281
Fragments arranged by size:
2037 1281 926
BlpI GC'TnA_GC
Cuts at: 1356 1356
Size: 4244
Bme1580I G_kGCm'C
Cuts at: 797 1869 1962 3018 797
Size: 1072 93 1056 2023
Fragments arranged by size:
2023 1072 1056 93
BmgBI CAC'GTC
Cuts at: 747 747
Size: 4244
BmrI ACTGGGnnnn_n'
Cuts at: 1456 1537 1872 4016 1456
Size: 81 335 2144 1684
Fragments arranged by size:
2144 1684 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 542 932 328
Size: 214 390 3640
Fragments arranged by size:
3640 390 214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2299 2791 3089 1420
Size: 879 492 298 2575
Fragments arranged by size:
2575 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3948 3948
Size: 4244
BsaAI yAC'GTr
Cuts at: 630 1597 630
Size: 967 3277
BsaBI GATnn'nnATC
Cuts at: 6 329 371 374 638 1758 3795 6
Size: 323 42 3 264 1120 2037 455
Fragments arranged by size:
2037 1120 455 323 264 42 3
BsaHI Gr'CG_yC
Cuts at: 1675 1923 2625 4121 1675
Size: 248 702 1496 1798
Fragments arranged by size:
1798 1496 702 248
BsaWI w'CCGG_w
Cuts at: 378 1433 1954 2632 2906 3053 3464 378
Size: 1055 521 678 274 147 411 1158
Fragments arranged by size:
1158 1055 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 644 674 700 730 644
Size: 30 26 30 4158
Fragments arranged by size:
4158 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 316 1028 1031 127
Size: 189 712 3 3340
Fragments arranged by size:
3340 712 189 3
BseYI C'CCAG_C
Cuts at: 414 897 916 1669 2427 3004 3500 414
Size: 483 19 753 758 577 496 1158
Fragments arranged by size:
1158 758 753 577 496 483 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 691 628
Size: 63 4181
BsiEI CG_ry'CG
Cuts at: 104 1786 1832 3040 104
Size: 1682 46 1208 1308
Fragments arranged by size:
1682 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1258 2036 2226 3018 934
Size: 324 778 190 792 2160
Fragments arranged by size:
2160 792 778 324 190
BsmI GAATG_Cn'
Cuts at: 231 670 231
Size: 439 3805
Bsp1286I G_dGCh'C
Cuts at: 797 934 1184 1258 1869 1962 2036 2226 2288
Size: 137 250 74 611 93 74 190 62
Cuts at: 2288 3018 797
Size: 730 2023
Fragments arranged by size:
2023 730 611 250 190 137 93 74
74 62
BspEI T'CCGG_A
Cuts at: 378 3464 378
Size: 3086 1158
BspHI T'CATG_A
Cuts at: 692 1107 3420 692
Size: 415 2313 1516
Fragments arranged by size:
2313 1516 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 1508 1810 2191 1294
Size: 214 302 381 3347
Fragments arranged by size:
3347 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1182 2156 1182
Size: 974 3270
BsrFI r'CCGG_y
Cuts at: 126 1163 2242 2423 2632 126
Size: 1037 1079 181 209 1738
Fragments arranged by size:
1738 1079 1037 209 181
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 1621
Size: 4244
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 655 885 655
Size: 230 4014
BstYI r'GATC_y
Cuts at: 1 206 639 833 921 1097 1269 1736 1759
Size: 205 433 194 88 176 172 467 23
Cuts at: 1759 2094 2340 3352 3438 3450 3796 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 433 346 335 246 205
194 176 172 88 86 23 12
BstZ17I GTA'TAC
Cuts at: 861 861
Size: 4244
BtgI C'CryG_G
Cuts at: 107 296 648 107
Size: 189 352 3703
Fragments arranged by size:
3703 352 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 380 2194 2375 2538 380
Size: 1814 181 163 2086
Fragments arranged by size:
2086 1814 181 163
BtsI GCAGTG_nn'
Cuts at: 253 890 1487 253
Size: 637 597 3010
Fragments arranged by size:
3010 637 597
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 200 308 1165 1829 2003 2394 2421 3766 200
Size: 108 857 664 174 391 27 1345 678
Fragments arranged by size:
1345 857 678 664 391 174 108 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 2452 2762 2908 4054 145
Size: 2307 310 146 1146 335
Fragments arranged by size:
2307 1146 335 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 443 542 932 1246 2069 2501 3248 3516
Size: 115 99 390 314 823 432 747 268
Cuts at: 3516 328
Size: 1056
Fragments arranged by size:
1056 823 747 432 390 314 268 115
99
EcoNI CCTnn'n_nnAGG
Cuts at: 766 766
Size: 4244
EcoO109I rG'GnC_Cy
Cuts at: 1383 1383
Size: 4244
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 1678 1926 2948 3927 1678
Size: 248 1022 979 1995
Fragments arranged by size:
1995 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 538 570 1089 1121 2086 2116 2118 2148 538
Size: 32 519 32 965 30 2 30 2634
Fragments arranged by size:
2634 965 519 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1218 2915 3099 1218
Size: 1697 184 2363
Fragments arranged by size:
2363 1697 184
MscI TGG'CCA
Cuts at: 202 310 2005 202
Size: 108 1695 2441
Fragments arranged by size:
2441 1695 108
MslI CAynn'nnrTG
Cuts at: 323 653 697 2233 2360 323
Size: 330 44 1536 127 2207
Fragments arranged by size:
2207 1536 330 127 44
MspA1I CmG'CkG
Cuts at: 472 897 1355 1669 2029 3042 3287 472
Size: 425 458 314 360 1013 245 1429
Fragments arranged by size:
1429 1013 458 425 360 314 245
NaeI GCC'GGC
Cuts at: 1165 2425 1165
Size: 1260 2984
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NcoI C'CATG_G
Cuts at: 107 296 648 107
Size: 189 352 3703
Fragments arranged by size:
3703 352 189
NdeI CA'TA_TG
Cuts at: 249 1125 249
Size: 876 3368
NgoMIV G'CCGG_C
Cuts at: 1163 2423 1163
Size: 1260 2984
NspI r_CATG'y
Cuts at: 1234 1297 1797 2704 1234
Size: 63 500 907 2774
Fragments arranged by size:
2774 907 500 63
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PshAI GACnn'nnGTC
Cuts at: 1066 1066
Size: 4244
PstI C_TGCA'G
Cuts at: 338 1291 1976 338
Size: 953 685 2606
Fragments arranged by size:
2606 953 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 472 897 1669 2029 472
Size: 425 772 360 2687
Fragments arranged by size:
2687 772 425 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 2267 2477 2267
Size: 210 4034
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 165 334 449 780 1287 1972 2965 3156
Size: 132 169 115 331 507 685 993 191
Cuts at: 3156 33
Size: 1121
Fragments arranged by size:
1121 993 685 507 331 191 169 132
115
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 970 1399 2314 2806 3068 970
Size: 429 915 492 262 2146
Fragments arranged by size:
2146 915 492 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
SspI AAT'ATT
Cuts at: 593 826 593
Size: 233 4011
StyI C'CwwG_G
Cuts at: 107 296 648 762 1074 1378 1732 107
Size: 189 352 114 312 304 354 2619
Fragments arranged by size:
2619 354 352 312 304 189 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1827 1960 2419 2628 1827
Size: 133 459 209 3443
Fragments arranged by size:
3443 459 209 133
TatI w'GTAC_w
Cuts at: 846 974 3759 846
Size: 128 2785 1331
Fragments arranged by size:
2785 1331 128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 700 732 913 945 3761 3793 700
Size: 32 181 32 2816 32 1151
Fragments arranged by size:
2816 1151 181 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 699 2041 699
Size: 1342 2902
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 303 1739 303
Size: 1436 2808
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PmeI PpiI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI