Restriction map for
pF1KB7641
(Circular) (Six-base) MAPSORT of: pF1KB7641.seq Check: 8638 from: 1 to: 4091
pF1KB7641 4091 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 692 776 692
Size: 84 4007
AccI GT'mk_AC
Cuts at: 1129 1129
Size: 4091
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1916 2348 3095 3363 1916
Size: 432 747 268 2644
Fragments arranged by size:
2644 747 432 268
AflIII A'CryG_T
Cuts at: 854 1443 2547 3524 854
Size: 589 1104 977 1421
Fragments arranged by size:
1421 1104 977 589
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 312 1396 2963 312
Size: 1084 1567 1440
Fragments arranged by size:
1567 1440 1084
ApaI G_GGCC'C
Cuts at: 208 469 818 1048 208
Size: 261 349 230 3251
Fragments arranged by size:
3251 349 261 230
ApaLI G'TGCA_C
Cuts at: 265 2861 265
Size: 2596 1495
ApoI r'AATT_y
Cuts at: 1095 3444 3700 1095
Size: 2349 256 1486
Fragments arranged by size:
2349 1486 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 259 630 649 708 811 921 1111 259
Size: 371 19 59 103 110 190 3239
Fragments arranged by size:
3239 371 190 110 103 59 19
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 120 167 444 514 540 600 634 773 968
Size: 47 277 70 26 60 34 139 195
Cuts at: 968 1107 1521 1769 1804 120
Size: 139 414 248 35 2407
Fragments arranged by size:
2407 414 277 248 195 139 139 70
60 47 35 34 26
BanII G_rGCy'C
Cuts at: 208 401 469 584 818 1048 1105 2135 208
Size: 193 68 115 234 230 57 1030 2164
Fragments arranged by size:
2164 1030 234 230 193 115 68 57
BbeI G_GCGC'C
Cuts at: 544 604 777 972 1525 1773 544
Size: 60 173 195 553 248 2862
Fragments arranged by size:
2862 553 248 195 173 60
BbvCI CC'TCA_GC
Cuts at: 530 530
Size: 4091
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 945 979 945
Size: 34 4057
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 1611 1611
Size: 4091
BglI GCCn_nnn'nGGC
Cuts at: 526 710 526
Size: 184 3907
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 1203 1203
Size: 4091
Bme1580I G_kGCm'C
Cuts at: 125 208 269 469 637 818 1048 1716 1809
Size: 83 61 200 168 181 230 668 93
Cuts at: 1809 2865 125
Size: 1056 1351
Fragments arranged by size:
1351 1056 668 230 200 181 168 93
83 61
BmgBI CAC'GTC
Cuts at: 641 641
Size: 4091
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1384 1719 3863 1303
Size: 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 464 2458 464
Size: 1994 2097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 412
Size: 4091
Bpu10I CC'TnA_GC
Cuts at: 313 530 313
Size: 217 3874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2146 2638 2936 1267
Size: 879 492 298 2422
Fragments arranged by size:
2422 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 4091
BsaAI yAC'GTr
Cuts at: 981 1444 981
Size: 463 3628
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 541 601 774 969 1522 1770 2472 3968 541
Size: 60 173 195 553 248 702 1496 664
Fragments arranged by size:
1496 702 664 553 248 195 173 60
BsaWI w'CCGG_w
Cuts at: 1280 1801 2479 2753 2900 3311 1280
Size: 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 268
Size: 30 4061
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 388 409 388
Size: 21 4070
BseYI C'CCAG_C
Cuts at: 219 583 944 1516 2274 2851 3347 219
Size: 364 361 572 758 577 496 963
Fragments arranged by size:
963 758 577 572 496 364 361
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 369
Size: 4091
BsiEI CG_ry'CG
Cuts at: 104 1008 1633 1679 2887 104
Size: 904 625 46 1208 1308
Fragments arranged by size:
1308 1208 904 625 46
BsiHKAI G_wGCw'C
Cuts at: 269 401 767 896 980 1105 1883 2073 2865
Size: 132 366 129 84 125 778 190 792
Cuts at: 2865 269
Size: 1495
Fragments arranged by size:
1495 792 778 366 190 132 129 125
84
Bsp1286I G_dGCh'C
Cuts at: 125 208 269 401 469 584 637 767 818
Size: 83 61 132 68 115 53 130 51
Cuts at: 818 896 980 1048 1105 1716 1809 1883 2073
Size: 78 84 68 57 611 93 74 190
Cuts at: 2073 2135 2865 125
Size: 62 730 1351
Fragments arranged by size:
1351 730 611 190 132 130 115 93
84 83 78 74 68 68 62 61
57 53 51
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 776 1141 1355 1657 2038 692
Size: 84 365 214 302 381 2745
Fragments arranged by size:
2745 381 365 302 214 84
BsrBI CCG'CTC
Cuts at: 50 368 2383 2437 3921 4086 50
Size: 318 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 318 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2003 2003
Size: 4091
BsrFI r'CCGG_y
Cuts at: 353 537 695 2089 2270 2479 353
Size: 184 158 1394 181 209 1965
Fragments arranged by size:
1965 1394 209 184 181 158
BssHII G'CGCG_C
Cuts at: 317 1022 317
Size: 705 3386
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 900 984 1468 900
Size: 84 484 3523
Fragments arranged by size:
3523 484 84
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4091
BstYI r'GATC_y
Cuts at: 1 1116 1583 1606 1941 2187 3199 3285 3297
Size: 1115 467 23 335 246 1012 86 12
Cuts at: 3297 3643 1
Size: 346 449
Fragments arranged by size:
1115 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 449 510 449
Size: 61 4030
BtgI C'CryG_G
Cuts at: 107 163 208 1002 107
Size: 56 45 794 3196
Fragments arranged by size:
3196 794 56 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2041 2222 2385 2041
Size: 181 163 3747
Fragments arranged by size:
3747 181 163
BtsI GCAGTG_nn'
Cuts at: 1334 1334
Size: 4091
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DraIII CAC_nnn'GTG
Cuts at: 900 900
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 1005 1016 1676 1850 2241 2268 3613 1005
Size: 11 660 174 391 27 1345 1483
Fragments arranged by size:
1483 1345 660 391 174 27 11
EagI C'GGCC_G
Cuts at: 1005 1676 1005
Size: 671 3420
EarI CTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 166 2299 2609 2755 3901 166
Size: 2133 310 146 1146 356
Fragments arranged by size:
2133 1146 356 310 146
EcoICRI GAG'CTC
Cuts at: 399 1103 399
Size: 704 3387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1916 2348 3095 3363 412
Size: 1504 432 747 268 1140
Fragments arranged by size:
1504 1140 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 391 391
Size: 4091
EcoO109I rG'GnC_Cy
Cuts at: 144 465 466 1060 1230 144
Size: 321 1 594 170 3005
Fragments arranged by size:
3005 594 321 170 1
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 154 220 544 604 777 972 1525 1773 2795
Size: 66 324 60 173 195 553 248 1022
Cuts at: 2795 3774 154
Size: 979 471
Fragments arranged by size:
1022 979 553 471 324 248 195 173
66 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 415 447 1933 1963 1965 1995 415
Size: 32 1486 30 2 30 2511
Fragments arranged by size:
2511 1486 32 30 30 2
HincII GTy'rAC
Cuts at: 1130 1130
Size: 4091
KasI G'GCGC_C
Cuts at: 540 600 773 968 1521 1769 540
Size: 60 173 195 553 248 2862
Fragments arranged by size:
2862 553 248 195 173 60
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 854 3524 854
Size: 2670 1421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 2762 2946 266
Size: 2496 184 1411
Fragments arranged by size:
2496 1411 184
MscI TGG'CCA
Cuts at: 1852 1852
Size: 4091
MslI CAynn'nnrTG
Cuts at: 112 2080 2207 112
Size: 1968 127 1996
Fragments arranged by size:
1996 1968 127
MspA1I CmG'CkG
Cuts at: 210 597 1015 1202 1516 1876 2889 3134 210
Size: 387 418 187 314 360 1013 245 1167
Fragments arranged by size:
1167 1013 418 387 360 314 245 187
NaeI GCC'GGC
Cuts at: 355 539 2272 355
Size: 184 1733 2174
Fragments arranged by size:
2174 1733 184
NarI GG'CG_CC
Cuts at: 541 601 774 969 1522 1770 541
Size: 60 173 195 553 248 2862
Fragments arranged by size:
2862 553 248 195 173 60
NcoI C'CATG_G
Cuts at: 107 107
Size: 4091
NgoMIV G'CCGG_C
Cuts at: 353 537 2270 353
Size: 184 1733 2174
Fragments arranged by size:
2174 1733 184
NspI r_CATG'y
Cuts at: 1144 1644 2551 1144
Size: 500 907 2684
Fragments arranged by size:
2684 907 500
PasI CC'CwG_GG
Cuts at: 469 523 469
Size: 54 4037
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PmlI CAC'GTG
Cuts at: 981 981
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PspOMI G'GGCC_C
Cuts at: 204 465 814 1044 204
Size: 261 349 230 3251
Fragments arranged by size:
3251 349 261 230
PstI C_TGCA'G
Cuts at: 1138 1823 1138
Size: 685 3406
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 1516 1876 1516
Size: 360 3731
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 401 1105 401
Size: 704 3387
SacII CC_GC'GG
Cuts at: 211 211
Size: 4091
SalI G'TCGA_C
Cuts at: 1128 1128
Size: 4091
SapI GCTCTTCn'nnn_
Cuts at: 2114 2324 2114
Size: 210 3881
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 3608 3608
Size: 4091
SexAI A'CCwGG_T
Cuts at: 896 896
Size: 4091
SfcI C'TryA_G
Cuts at: 33 1134 1819 2812 3003 33
Size: 1101 685 993 191 1121
Fragments arranged by size:
1121 1101 993 685 191
SfoI GGC'GCC
Cuts at: 542 602 775 970 1523 1771 542
Size: 60 173 195 553 248 2862
Fragments arranged by size:
2862 553 248 195 173 60
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 632 813 1113 632
Size: 181 300 3610
Fragments arranged by size:
3610 300 181
SmlI C'TyrA_G
Cuts at: 1246 2161 2653 2915 1246
Size: 915 492 262 2422
Fragments arranged by size:
2422 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
StuI AGG'CCT
Cuts at: 340 748 340
Size: 408 3683
StyI C'CwwG_G
Cuts at: 107 272 1079 1225 1579 107
Size: 165 807 146 354 2619
Fragments arranged by size:
2619 807 354 165 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 333 1072 1674 1807 2266 2475 333
Size: 739 602 133 459 209 1949
Fragments arranged by size:
1949 739 602 459 209 133
TatI w'GTAC_w
Cuts at: 3606 3606
Size: 4091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 148 180 3608 3640 148
Size: 32 3428 32 599
Fragments arranged by size:
3428 599 32 32
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 630 811 1111 630
Size: 181 300 3610
Fragments arranged by size:
3610 300 181
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PmlI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BsiWI BsmI BsmBI
BsrGI BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PpuMI PshAI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI