Restriction map for
pF1KB7648
(Circular) (Six-base) MAPSORT of: pF1KB7648.seq Check: 7721 from: 1 to: 4289
pF1KB7648 4289 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1327 1327
Size: 4289
Acc65I G'GTAC_C
Cuts at: 1240 1305 1240
Size: 65 4224
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 2114 2546 3293 3561 199
Size: 1915 432 747 268 927
Fragments arranged by size:
1915 927 747 432 268
AfeI AGC'GCT
Cuts at: 451 451
Size: 4289
AflIII A'CryG_T
Cuts at: 1131 1641 2745 3722 1131
Size: 510 1104 977 1698
Fragments arranged by size:
1698 1104 977 510
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 1594 3161 1594
Size: 1567 2722
ApaLI G'TGCA_C
Cuts at: 3059 3059
Size: 4289
ApoI r'AATT_y
Cuts at: 1293 3642 3898 1293
Size: 2349 256 1684
Fragments arranged by size:
2349 1684 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 791 1309 791
Size: 518 3771
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 1240 1305 1719 1967 2002 1240
Size: 65 414 248 35 3527
Fragments arranged by size:
3527 414 248 65 35
BanII G_rGCy'C
Cuts at: 1303 2333 1303
Size: 1030 3259
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BbsI GAAGACnn'nnnn_
Cuts at: 357 808 357
Size: 451 3838
BbvCI CC'TCA_GC
Cuts at: 212 212
Size: 4289
BciVI GTATCCnnnnn_n'
Cuts at: 2187 2948 4289 2187
Size: 761 1341 2187
Fragments arranged by size:
2187 1341 761
BclI T'GATC_A
Cuts at: 1809 1809
Size: 4289
BglI GCCn_nnn'nGGC
Cuts at: 856 856
Size: 4289
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 1914 2007 3063 1914
Size: 93 1056 3140
Fragments arranged by size:
3140 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 438 943 1501 1582 1917 4061 438
Size: 505 558 81 335 2144 666
Fragments arranged by size:
2144 666 558 505 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1185 1200 2656 1185
Size: 15 1456 2818
Fragments arranged by size:
2818 1456 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 862 679
Size: 183 4106
Bpu10I CC'TnA_GC
Cuts at: 212 212
Size: 4289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2344 2836 3134 1465
Size: 879 492 298 2620
Fragments arranged by size:
2620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3993 3993
Size: 4289
BsaAI yAC'GTr
Cuts at: 1642 1642
Size: 4289
BsaBI GATnn'nnATC
Cuts at: 6 1803 3840 6
Size: 1797 2037 455
Fragments arranged by size:
2037 1797 455
BsaHI Gr'CG_yC
Cuts at: 1720 1968 2670 4166 1720
Size: 248 702 1496 1843
Fragments arranged by size:
1843 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1478 1999 2677 2951 3098 3509 1478
Size: 521 678 274 147 411 2258
Fragments arranged by size:
2258 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 652 682 652
Size: 30 4259
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 1192 205
Size: 987 3302
BseYI C'CCAG_C
Cuts at: 980 1111 1714 2472 3049 3545 980
Size: 131 603 758 577 496 1724
Fragments arranged by size:
1724 758 603 577 496 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 721
Size: 4289
BsiEI CG_ry'CG
Cuts at: 104 1831 1877 3085 104
Size: 1727 46 1208 1308
Fragments arranged by size:
1727 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1303 2081 2271 3063 1303
Size: 778 190 792 2529
Fragments arranged by size:
2529 792 778 190
BsmI GAATG_Cn'
Cuts at: 443 443
Size: 4289
Bsp1286I G_dGCh'C
Cuts at: 1303 1914 2007 2081 2271 2333 3063 1303
Size: 611 93 74 190 62 730 2529
Fragments arranged by size:
2529 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 819 3465 819
Size: 2646 1643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1339 1553 1855 2236 1339
Size: 214 302 381 3392
Fragments arranged by size:
3392 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2581 2635 4119 4284 50
Size: 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 597 2201 597
Size: 1604 2685
BsrFI r'CCGG_y
Cuts at: 2287 2468 2677 2287
Size: 181 209 3899
Fragments arranged by size:
3899 209 181
BssSI C'ACGA_G
Cuts at: 2560 2918 2560
Size: 358 3931
BstAPI GCAn_nnn'nTGC
Cuts at: 865 1666 865
Size: 801 3488
BstBI TT'CG_AA
Cuts at: 438 2650 438
Size: 2212 2077
BstXI CCAn_nnnn'nTGG
Cuts at: 423 933 423
Size: 510 3779
BstYI r'GATC_y
Cuts at: 1 116 1314 1781 1804 2139 2385 3397 3483
Size: 115 1198 467 23 335 246 1012 86
Cuts at: 3483 3495 3841 1
Size: 12 346 449
Fragments arranged by size:
1198 1012 467 449 346 335 246 115
86 23 12
BtgI C'CryG_G
Cuts at: 107 911 1274 107
Size: 804 363 3122
Fragments arranged by size:
3122 804 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 368 1211 1532 368
Size: 843 321 3125
Fragments arranged by size:
3125 843 321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 954 989 954
Size: 35 4254
DraI TTT'AAA
Cuts at: 1288 1288
Size: 4289
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 1874 2048 2439 2466 3811 1874
Size: 174 391 27 1345 2352
Fragments arranged by size:
2352 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1874 1874
Size: 4289
EarI CTCTTCn'nnn_
Cuts at: 842 2312 2522 842
Size: 1470 210 2609
Fragments arranged by size:
2609 1470 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 187 329 2497 2807 2953 4099 187
Size: 142 2168 310 146 1146 377
Fragments arranged by size:
2168 1146 377 310 146 142
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 679 862 2114 2546 3293 3561 199
Size: 480 183 1252 432 747 268 927
Fragments arranged by size:
1252 927 747 480 432 268 183
EcoO109I rG'GnC_Cy
Cuts at: 216 1428 216
Size: 1212 3077
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
EcoRV GAT'ATC
Cuts at: 705 705
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 4289
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 453 1723 1971 2993 3972 453
Size: 1270 248 1022 979 770
Fragments arranged by size:
1270 1022 979 770 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 675 707 1147 1179 2131 2161 2163 2193 675
Size: 32 440 32 952 30 2 30 2771
Fragments arranged by size:
2771 952 440 32 32 30 30 2
HincII GTy'rAC
Cuts at: 780 1328 780
Size: 548 3741
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 1244 1309 1244
Size: 65 4224
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 972 2960 3144 972
Size: 1988 184 2117
Fragments arranged by size:
2117 1988 184
MscI TGG'CCA
Cuts at: 2050 2050
Size: 4289
MslI CAynn'nnrTG
Cuts at: 389 2278 2405 389
Size: 1889 127 2273
Fragments arranged by size:
2273 1889 127
MspA1I CmG'CkG
Cuts at: 829 1400 1714 2074 3087 3332 829
Size: 571 314 360 1013 245 1786
Fragments arranged by size:
1786 1013 571 360 314 245
NaeI GCC'GGC
Cuts at: 2470 2470
Size: 4289
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 107 1274 107
Size: 1167 3122
NdeI CA'TA_TG
Cuts at: 774 774
Size: 4289
NgoMIV G'CCGG_C
Cuts at: 2468 2468
Size: 4289
NspI r_CATG'y
Cuts at: 516 1135 1342 1842 2749 516
Size: 619 207 500 907 2056
Fragments arranged by size:
2056 907 619 500 207
PciI A'CATG_T
Cuts at: 1131 2745 1131
Size: 1614 2675
PfoI T'CCnGG_A
Cuts at: 656 1194 656
Size: 538 3751
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PpuMI rG'GwC_Cy
Cuts at: 216 216
Size: 4289
PstI C_TGCA'G
Cuts at: 1336 2021 1336
Size: 685 3604
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 829 1714 2074 829
Size: 885 360 3044
Fragments arranged by size:
3044 885 360
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 842 2312 2522 842
Size: 1470 210 2609
Fragments arranged by size:
2609 1470 210
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 1332 2017 3010 3201 33
Size: 1299 685 993 191 1121
Fragments arranged by size:
1299 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 1311 1311
Size: 4289
SmlI C'TyrA_G
Cuts at: 1444 2359 2851 3113 1444
Size: 915 492 262 2620
Fragments arranged by size:
2620 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
StuI AGG'CCT
Cuts at: 410 410
Size: 4289
StyI C'CwwG_G
Cuts at: 107 1139 1274 1423 1777 107
Size: 1032 135 149 354 2619
Fragments arranged by size:
2619 1032 354 149 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 942 1872 2005 2464 2673 942
Size: 930 133 459 209 2558
Fragments arranged by size:
2558 930 459 209 133
TatI w'GTAC_w
Cuts at: 1038 3804 1038
Size: 2766 1523
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4257
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 351 1784 351
Size: 1433 2856
XmaI C'CCGG_G
Cuts at: 1309 1309
Size: 4289
XmnI GAAnn'nnTTC
Cuts at: 1054 1054
Size: 4289
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI