Restriction map for
pF1KB7653
(Circular) (Six-base) MAPSORT of: pF1KB7653.seq Check: 6338 from: 1 to: 4115
pF1KB7653 4115 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 986 1940 2372 3119 3387 227
Size: 759 954 432 747 268 955
Fragments arranged by size:
955 954 759 747 432 268
AflIII A'CryG_T
Cuts at: 1467 2571 3548 1467
Size: 1104 977 2034
Fragments arranged by size:
2034 1104 977
AgeI A'CCGG_T
Cuts at: 2503 2503
Size: 4115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 936 936
Size: 4115
AlwNI CAG_nnn'CTG
Cuts at: 630 759 1420 2987 630
Size: 129 661 1567 1758
Fragments arranged by size:
1758 1567 661 129
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 163 709 1119 3468 3724 163
Size: 546 410 2349 256 554
Fragments arranged by size:
2349 554 546 410 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 1135 1135
Size: 4115
BamHI G'GATC_C
Cuts at: 1140 1140
Size: 4115
BanI G'GyrC_C
Cuts at: 121 1131 1545 1793 1828 121
Size: 1010 414 248 35 2408
Fragments arranged by size:
2408 1010 414 248 35
BanII G_rGCy'C
Cuts at: 1003 1076 1129 2159 1003
Size: 73 53 1030 2959
Fragments arranged by size:
2959 1030 73 53
BbeI G_GCGC'C
Cuts at: 125 1549 1797 125
Size: 1424 248 2443
Fragments arranged by size:
2443 1424 248
BbsI GAAGACnn'nnnn_
Cuts at: 963 963
Size: 4115
BbvCI CC'TCA_GC
Cuts at: 128 128
Size: 4115
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglII A'GATC_T
Cuts at: 1083 1630 3667 1083
Size: 547 2037 1531
Fragments arranged by size:
2037 1531 547
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 1740 1833 2889 1740
Size: 93 1056 2966
Fragments arranged by size:
2966 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1327 1408 1743 3887 1327
Size: 81 335 2144 1555
Fragments arranged by size:
2144 1555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 495 1117 280
Size: 215 622 3278
Fragments arranged by size:
3278 622 215
Bpu10I CC'TnA_GC
Cuts at: 128 128
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 2170 2662 2960 1291
Size: 879 492 298 2446
Fragments arranged by size:
2446 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3819 3819
Size: 4115
BsaAI yAC'GTr
Cuts at: 1468 1468
Size: 4115
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 122 1546 1794 2496 3992 122
Size: 1424 248 702 1496 245
Fragments arranged by size:
1496 1424 702 248 245
BsaWI w'CCGG_w
Cuts at: 995 1304 1825 2503 2777 2924 3335 995
Size: 309 521 678 274 147 411 1775
Fragments arranged by size:
1775 678 521 411 309 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 560 145
Size: 415 3700
BseYI C'CCAG_C
Cuts at: 1540 2298 2875 3371 1540
Size: 758 577 496 2284
Fragments arranged by size:
2284 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 391
Size: 4115
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2911 104
Size: 1553 46 1208 1308
Fragments arranged by size:
1553 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 737 1003 1076 1129 1907 2097 2889 347
Size: 390 266 73 53 778 190 792 1573
Fragments arranged by size:
1573 792 778 390 266 190 73 53
Bsp1286I G_dGCh'C
Cuts at: 347 737 1003 1076 1129 1740 1833 1907 2097
Size: 390 266 73 53 611 93 74 190
Cuts at: 2097 2159 2889 347
Size: 62 730 1573
Fragments arranged by size:
1573 730 611 390 266 190 93 74
73 62 53
BspEI T'CCGG_A
Cuts at: 995 3335 995
Size: 2340 1775
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1165 1379 1681 2062 1165
Size: 214 302 381 3218
Fragments arranged by size:
3218 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2407 2461 3945 4110 50
Size: 2357 54 1484 165 55
Fragments arranged by size:
2357 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1034 2027 1034
Size: 993 3122
BsrFI r'CCGG_y
Cuts at: 140 2113 2294 2503 140
Size: 1973 181 209 1752
Fragments arranged by size:
1973 1752 209 181
BsrGI T'GTAC_A
Cuts at: 466 466
Size: 4115
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 447 1492 447
Size: 1045 3070
BstBI TT'CG_AA
Cuts at: 2476 2476
Size: 4115
BstYI r'GATC_y
Cuts at: 1 186 1083 1140 1607 1630 1965 2211 3223
Size: 185 897 57 467 23 335 246 1012
Cuts at: 3223 3309 3321 3667 1
Size: 86 12 346 449
Fragments arranged by size:
1012 897 467 449 346 335 246 185
86 57 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2065 2246 2409 2065
Size: 181 163 3771
Fragments arranged by size:
3771 181 163
BtsI GCAGTG_nn'
Cuts at: 522 1358 522
Size: 836 3279
DraI TTT'AAA
Cuts at: 351 1114 351
Size: 763 3352
DrdI GACnn_nn'nnGTC
Cuts at: 619 1821 2679 619
Size: 1202 858 2055
Fragments arranged by size:
2055 1202 858
EaeI y'GGCC_r
Cuts at: 1700 1874 2265 2292 3637 1700
Size: 174 391 27 1345 2178
Fragments arranged by size:
2178 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 416 2138 2348 416
Size: 1722 210 2183
Fragments arranged by size:
2183 1722 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 2633 2779 3925 2323
Size: 310 146 1146 2513
Fragments arranged by size:
2513 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1001 1074 1127 1001
Size: 73 53 3989
Fragments arranged by size:
3989 73 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 280 495 986 1117 1940 2372 3119 3387
Size: 53 215 491 131 823 432 747 268
Cuts at: 3387 227
Size: 955
Fragments arranged by size:
955 823 747 491 432 268 215 131
53
EcoNI CCTnn'n_nnAGG
Cuts at: 451 451
Size: 4115
EcoO109I rG'GnC_Cy
Cuts at: 1254 1254
Size: 4115
EcoRI G'AATT_C
Cuts at: 709 1119 709
Size: 410 3705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 4115
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 125 1549 1797 2819 3798 125
Size: 1424 248 1022 979 442
Fragments arranged by size:
1424 1022 979 442 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1957 1987 1989 2019 1957
Size: 30 2 30 4053
Fragments arranged by size:
4053 30 30 2
HincII GTy'rAC
Cuts at: 1154 1154
Size: 4115
HindIII A'AGCT_T
Cuts at: 554 554
Size: 4115
KasI G'GCGC_C
Cuts at: 121 1545 1793 121
Size: 1424 248 2443
Fragments arranged by size:
2443 1424 248
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2786 2970 2786
Size: 184 3931
MscI TGG'CCA
Cuts at: 1876 1876
Size: 4115
MslI CAynn'nnrTG
Cuts at: 2104 2231 2104
Size: 127 3988
MspA1I CmG'CkG
Cuts at: 127 327 1226 1540 1900 2913 3158 127
Size: 200 899 314 360 1013 245 1084
Fragments arranged by size:
1084 1013 899 360 314 245 200
NaeI GCC'GGC
Cuts at: 142 2296 142
Size: 2154 1961
NarI GG'CG_CC
Cuts at: 122 1546 1794 122
Size: 1424 248 2443
Fragments arranged by size:
2443 1424 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 140 2294 140
Size: 2154 1961
NspI r_CATG'y
Cuts at: 701 1168 1668 2575 701
Size: 467 500 907 2241
Fragments arranged by size:
2241 907 500 467
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PflMI CCAn_nnn'nTGG
Cuts at: 630 951 630
Size: 321 3794
PfoI T'CCnGG_A
Cuts at: 623 867 1076 623
Size: 244 209 3662
Fragments arranged by size:
3662 244 209
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PstI C_TGCA'G
Cuts at: 452 1162 1847 452
Size: 710 685 2720
Fragments arranged by size:
2720 710 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 327 1540 1900 327
Size: 1213 360 2542
Fragments arranged by size:
2542 1213 360
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 1003 1076 1129 1003
Size: 73 53 3989
Fragments arranged by size:
3989 73 53
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 416 2138 2348 416
Size: 1722 210 2183
Fragments arranged by size:
2183 1722 210
SbfI CC_TGCA'GG
Cuts at: 1162 1162
Size: 4115
ScaI AGT'ACT
Cuts at: 3632 3632
Size: 4115
SfcI C'TryA_G
Cuts at: 33 448 692 1158 1843 2836 3027 33
Size: 415 244 466 685 993 191 1121
Fragments arranged by size:
1121 993 685 466 415 244 191
SfoI GGC'GCC
Cuts at: 123 1547 1795 123
Size: 1424 248 2443
Fragments arranged by size:
2443 1424 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 1137 1137
Size: 4115
SmlI C'TyrA_G
Cuts at: 1270 2185 2677 2939 1270
Size: 915 492 262 2446
Fragments arranged by size:
2446 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 1168 1668 1168
Size: 500 3615
StuI AGG'CCT
Cuts at: 136 136
Size: 4115
StyI C'CwwG_G
Cuts at: 107 1249 1603 107
Size: 1142 354 2619
Fragments arranged by size:
2619 1142 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1698 1831 2290 2499 1698
Size: 133 459 209 3314
Fragments arranged by size:
3314 459 209 133
TatI w'GTAC_w
Cuts at: 466 930 3630 466
Size: 464 2700 951
Fragments arranged by size:
2700 951 464
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1610 1610
Size: 4115
XmaI C'CCGG_G
Cuts at: 1135 1135
Size: 4115
XmnI GAAnn'nnTTC
Cuts at: 565 565
Size: 4115
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI