Restriction map for
pF1KB7663
(Circular) (Six-base) MAPSORT of: pF1KB7663.seq Check: 8197 from: 1 to: 4121
pF1KB7663 4121 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 240 979 1159 240
Size: 739 180 3202
Fragments arranged by size:
3202 739 180
Acc65I G'GTAC_C
Cuts at: 1137 1137
Size: 4121
AclI AA'CG_TT
Cuts at: 3961 3961
Size: 4121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1946 2378 3125 3393 571
Size: 1375 432 747 268 1299
Fragments arranged by size:
1375 1299 747 432 268
AflIII A'CryG_T
Cuts at: 1473 2577 3554 1473
Size: 1104 977 2040
Fragments arranged by size:
2040 1104 977
AgeI A'CCGG_T
Cuts at: 2509 2509
Size: 4121
AlwNI CAG_nnn'CTG
Cuts at: 1426 2993 1426
Size: 1567 2554
ApaLI G'TGCA_C
Cuts at: 833 2891 833
Size: 2058 2063
ApoI r'AATT_y
Cuts at: 443 1125 3474 3730 443
Size: 682 2349 256 834
Fragments arranged by size:
2349 834 682 256
AseI AT'TA_AT
Cuts at: 20 253 20
Size: 233 3888
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
AvaI C'yCGr_G
Cuts at: 1141 1141
Size: 4121
BamHI G'GATC_C
Cuts at: 1146 1146
Size: 4121
BanI G'GyrC_C
Cuts at: 1137 1551 1799 1834 1137
Size: 414 248 35 3424
Fragments arranged by size:
3424 414 248 35
BanII G_rGCy'C
Cuts at: 1135 2165 1135
Size: 1030 3091
BbeI G_GCGC'C
Cuts at: 1555 1803 1555
Size: 248 3873
BciVI GTATCCnnnnn_n'
Cuts at: 2019 2780 4121 2019
Size: 761 1341 2019
Fragments arranged by size:
2019 1341 761
BclI T'GATC_A
Cuts at: 876 1641 876
Size: 765 3356
BfrBI ATG'CAT
Cuts at: 652 652
Size: 4121
BglII A'GATC_T
Cuts at: 1636 3673 1636
Size: 2037 2084
BlpI GC'TnA_GC
Cuts at: 1233 1233
Size: 4121
Bme1580I G_kGCm'C
Cuts at: 262 837 1746 1839 2895 262
Size: 575 909 93 1056 1488
Fragments arranged by size:
1488 1056 909 575 93
BmrI ACTGGGnnnn_n'
Cuts at: 1333 1414 1749 3893 1333
Size: 81 335 2144 1561
Fragments arranged by size:
2144 1561 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 2176 2668 2966 1297
Size: 879 492 298 2452
Fragments arranged by size:
2452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3825 3825
Size: 4121
BsaAI yAC'GTr
Cuts at: 1474 1474
Size: 4121
BsaBI GATnn'nnATC
Cuts at: 6 1635 3672 6
Size: 1629 2037 455
Fragments arranged by size:
2037 1629 455
BsaHI Gr'CG_yC
Cuts at: 1552 1800 2502 3998 1552
Size: 248 702 1496 1675
Fragments arranged by size:
1675 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1310 1831 2509 2783 2930 3341 1310
Size: 521 678 274 147 411 2090
Fragments arranged by size:
2090 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1546 2304 2881 3377 1546
Size: 758 577 496 2290
Fragments arranged by size:
2290 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 344 142
Size: 202 3919
BsiEI CG_ry'CG
Cuts at: 104 1663 1709 2917 104
Size: 1559 46 1208 1308
Fragments arranged by size:
1559 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 837 1112 1135 1913 2103 2895 837
Size: 275 23 778 190 792 2063
Fragments arranged by size:
2063 792 778 275 190 23
Bsp1286I G_dGCh'C
Cuts at: 262 837 1112 1135 1746 1839 1913 2103 2165
Size: 575 275 23 611 93 74 190 62
Cuts at: 2165 2895 262
Size: 730 1488
Fragments arranged by size:
1488 730 611 575 275 190 93 74
62 23
BspEI T'CCGG_A
Cuts at: 3341 3341
Size: 4121
BspHI T'CATG_A
Cuts at: 3297 3297
Size: 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1171 1385 1687 2068 1171
Size: 214 302 381 3224
Fragments arranged by size:
3224 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2413 2467 3951 4116 50
Size: 2363 54 1484 165 55
Fragments arranged by size:
2363 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 752 2033 752
Size: 1281 2840
BsrFI r'CCGG_y
Cuts at: 2119 2300 2509 2119
Size: 181 209 3731
Fragments arranged by size:
3731 209 181
BssSI C'ACGA_G
Cuts at: 2392 2750 2392
Size: 358 3763
BstAPI GCAn_nnn'nTGC
Cuts at: 1498 1498
Size: 4121
BstBI TT'CG_AA
Cuts at: 924 2482 924
Size: 1558 2563
BstXI CCAn_nnnn'nTGG
Cuts at: 816 894 816
Size: 78 4043
BstYI r'GATC_y
Cuts at: 1 1146 1613 1636 1971 2217 3229 3315 3327
Size: 1145 467 23 335 246 1012 86 12
Cuts at: 3327 3673 1
Size: 346 449
Fragments arranged by size:
1145 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 980 980
Size: 4121
Bsu36I CC'TnA_GG
Cuts at: 191 191
Size: 4121
BtgI C'CryG_G
Cuts at: 1079 1079
Size: 4121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2071 2252 2415 2071
Size: 181 163 3777
Fragments arranged by size:
3777 181 163
BtsI GCAGTG_nn'
Cuts at: 639 1364 639
Size: 725 3396
DraI TTT'AAA
Cuts at: 1120 1120
Size: 4121
DrdI GACnn_nn'nnGTC
Cuts at: 1827 2685 1827
Size: 858 3263
EaeI y'GGCC_r
Cuts at: 1005 1706 1880 2271 2298 3643 1005
Size: 701 174 391 27 1345 1483
Fragments arranged by size:
1483 1345 701 391 174 27
EagI C'GGCC_G
Cuts at: 1706 1706
Size: 4121
EarI CTCTTCn'nnn_
Cuts at: 2144 2354 2144
Size: 210 3911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2329 2639 2785 3931 2329
Size: 310 146 1146 2519
Fragments arranged by size:
2519 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1133 1133
Size: 4121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1946 2378 3125 3393 571
Size: 1375 432 747 268 1299
Fragments arranged by size:
1375 1299 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 679 1260 679
Size: 581 3540
EcoRI G'AATT_C
Cuts at: 1125 1125
Size: 4121
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 4121
FspI TGC'GCA
Cuts at: 1902 1902
Size: 4121
HaeII r_GCGC'y
Cuts at: 1555 1803 2825 3804 1555
Size: 248 1022 979 1872
Fragments arranged by size:
1872 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 868 900 1963 1993 1995 2025 868
Size: 32 1063 30 2 30 2964
Fragments arranged by size:
2964 1063 32 30 30 2
HincII GTy'rAC
Cuts at: 1160 1160
Size: 4121
KasI G'GCGC_C
Cuts at: 1551 1799 1551
Size: 248 3873
KpnI G_GTAC'C
Cuts at: 1141 1141
Size: 4121
MfeI C'AATT_G
Cuts at: 752 752
Size: 4121
MluI A'CGCG_T
Cuts at: 3554 3554
Size: 4121
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 358 2792 2976 358
Size: 2434 184 1503
Fragments arranged by size:
2434 1503 184
MscI TGG'CCA
Cuts at: 1007 1882 1007
Size: 875 3246
MslI CAynn'nnrTG
Cuts at: 814 2110 2237 814
Size: 1296 127 2698
Fragments arranged by size:
2698 1296 127
MspA1I CmG'CkG
Cuts at: 1232 1546 1906 2919 3164 1232
Size: 314 360 1013 245 2189
Fragments arranged by size:
2189 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 4121
NarI GG'CG_CC
Cuts at: 1552 1800 1552
Size: 248 3873
NcoI C'CATG_G
Cuts at: 1079 1079
Size: 4121
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 4121
NsiI A_TGCA'T
Cuts at: 654 654
Size: 4121
NspI r_CATG'y
Cuts at: 1174 1674 2581 1174
Size: 500 907 2714
Fragments arranged by size:
2714 907 500
PciI A'CATG_T
Cuts at: 2577 2577
Size: 4121
PmeI GTTT'AAAC
Cuts at: 1120 1120
Size: 4121
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3260 3292 3260
Size: 32 4089
PstI C_TGCA'G
Cuts at: 578 646 1168 1853 578
Size: 68 522 685 2846
Fragments arranged by size:
2846 685 522 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4121
PvuII CAG'CTG
Cuts at: 1546 1906 1546
Size: 360 3761
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 4121
SacI G_AGCT'C
Cuts at: 1135 1135
Size: 4121
SalI G'TCGA_C
Cuts at: 1158 1158
Size: 4121
SapI GCTCTTCn'nnn_
Cuts at: 2144 2354 2144
Size: 210 3911
SbfI CC_TGCA'GG
Cuts at: 1168 1168
Size: 4121
ScaI AGT'ACT
Cuts at: 3638 3638
Size: 4121
SfcI C'TryA_G
Cuts at: 33 574 642 1164 1849 2842 3033 33
Size: 541 68 522 685 993 191 1121
Fragments arranged by size:
1121 993 685 541 522 191 68
SfoI GGC'GCC
Cuts at: 1553 1801 1553
Size: 248 3873
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4121
SmaI CCC'GGG
Cuts at: 1143 1143
Size: 4121
SmlI C'TyrA_G
Cuts at: 1276 2191 2683 2945 1276
Size: 915 492 262 2452
Fragments arranged by size:
2452 915 492 262
SpeI A'CTAG_T
Cuts at: 67 387 67
Size: 320 3801
SphI G_CATG'C
Cuts at: 1174 1674 1174
Size: 500 3621
StuI AGG'CCT
Cuts at: 801 801
Size: 4121
StyI C'CwwG_G
Cuts at: 1079 1255 1609 1079
Size: 176 354 3591
Fragments arranged by size:
3591 354 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 1837 2296 2505 1704
Size: 133 459 209 3320
Fragments arranged by size:
3320 459 209 133
TatI w'GTAC_w
Cuts at: 3636 3636
Size: 4121
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4089
Tth111I GACn'n_nGTC
Cuts at: 1918 1918
Size: 4121
XbaI T'CTAG_A
Cuts at: 1152 1152
Size: 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1616 1616
Size: 4121
XmaI C'CCGG_G
Cuts at: 1141 1141
Size: 4121
XmnI GAAnn'nnTTC
Cuts at: 920 920
Size: 4121
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI