Restriction map for
pF1KB7676
(Circular) (Six-base) MAPSORT of: pF1KB7676.seq Check: 7382 from: 1 to: 4331
pF1KB7676 4331 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 1246 1246
Size: 4331
AccI GT'mk_AC
Cuts at: 596 1114 1369 596
Size: 518 255 3558
Fragments arranged by size:
3558 518 255
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2588 3335 3603 2156
Size: 432 747 268 2884
Fragments arranged by size:
2884 747 432 268
AfeI AGC'GCT
Cuts at: 448 448
Size: 4331
AflIII A'CryG_T
Cuts at: 541 1683 2787 3764 541
Size: 1142 1104 977 1108
Fragments arranged by size:
1142 1108 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AleI CACnn'nnGTG
Cuts at: 1102 1102
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 1636 3203 1636
Size: 1567 2764
ApaI G_GGCC'C
Cuts at: 133 805 1153 133
Size: 672 348 3311
Fragments arranged by size:
3311 672 348
ApaLI G'TGCA_C
Cuts at: 139 534 3101 139
Size: 395 2567 1369
Fragments arranged by size:
2567 1369 395
ApoI r'AATT_y
Cuts at: 1335 3684 3940 1335
Size: 2349 256 1726
Fragments arranged by size:
2349 1726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 133 500 798 888 1058 1351 133
Size: 367 298 90 170 293 3113
Fragments arranged by size:
3113 367 298 293 170 90
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 1347 1761 2009 2044 1347
Size: 414 248 35 3634
Fragments arranged by size:
3634 414 248 35
BanII G_rGCy'C
Cuts at: 133 380 799 805 1153 1166 1345 2375 133
Size: 247 419 6 348 13 179 1030 2089
Fragments arranged by size:
2089 1030 419 348 247 179 13 6
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 425 730 907 425
Size: 305 177 3849
Fragments arranged by size:
3849 305 177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 467 664 691 467
Size: 197 27 4107
Fragments arranged by size:
4107 197 27
BglII A'GATC_T
Cuts at: 284 1846 3883 284
Size: 1562 2037 732
Fragments arranged by size:
2037 1562 732
BlpI GC'TnA_GC
Cuts at: 838 1180 1443 838
Size: 342 263 3726
Fragments arranged by size:
3726 342 263
Bme1580I G_kGCm'C
Cuts at: 133 143 538 763 805 1153 1956 2049 3105
Size: 10 395 225 42 348 803 93 1056
Cuts at: 3105 133
Size: 1359
Fragments arranged by size:
1359 1056 803 395 348 225 93 42
10
BmgBI CAC'GTC
Cuts at: 663 920 663
Size: 257 4074
BmrI ACTGGGnnnn_n'
Cuts at: 1543 1624 1959 4103 1543
Size: 81 335 2144 1771
Fragments arranged by size:
2144 1771 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 200 2698 200
Size: 2498 1833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1021 1173 1294 388
Size: 633 152 121 3425
Fragments arranged by size:
3425 633 152 121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1507 2386 2878 3176 325
Size: 1182 879 492 298 1480
Fragments arranged by size:
1480 1182 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4035 4035
Size: 4331
BsaAI yAC'GTr
Cuts at: 533 542 1684 533
Size: 9 1142 3180
Fragments arranged by size:
3180 1142 9
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 983 1141 1243 1762 2010 2712 4208 983
Size: 158 102 519 248 702 1496 1106
Fragments arranged by size:
1496 1106 702 519 248 158 102
BsaWI w'CCGG_w
Cuts at: 1520 2041 2719 2993 3140 3551 1520
Size: 521 678 274 147 411 2300
Fragments arranged by size:
2300 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 708 1756 2514 3091 3587 708
Size: 1048 758 577 496 1452
Fragments arranged by size:
1452 1048 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 787 463
Size: 324 4007
BsiEI CG_ry'CG
Cuts at: 104 210 677 781 1873 1919 3127 104
Size: 106 467 104 1092 46 1208 1308
Fragments arranged by size:
1308 1208 1092 467 106 104 46
BsiHKAI G_wGCw'C
Cuts at: 143 380 538 866 1166 1345 2123 2313 3105
Size: 237 158 328 300 179 778 190 792
Cuts at: 3105 143
Size: 1369
Fragments arranged by size:
1369 792 778 328 300 237 190 179
158
BsmBI CGTCTCn'nnnn_
Cuts at: 217 217
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 133 143 380 538 763 799 805 866 1153
Size: 10 237 158 225 36 6 61 287
Cuts at: 1153 1166 1345 1956 2049 2123 2313 2375 3105
Size: 13 179 611 93 74 190 62 730
Cuts at: 3105 133
Size: 1359
Fragments arranged by size:
1359 730 611 287 237 225 190 179
158 93 74 62 61 36 13 10
6
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 311 1381 1595 1897 2278 311
Size: 1070 214 302 381 2364
Fragments arranged by size:
2364 1070 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 268 2329 2510 2719 268
Size: 2061 181 209 1880
Fragments arranged by size:
2061 1880 209 181
BssHII G'CGCG_C
Cuts at: 495 495
Size: 4331
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 410 1708 410
Size: 1298 3033
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 732 732
Size: 4331
BstYI r'GATC_y
Cuts at: 1 284 813 1356 1823 1846 2181 2427 3439
Size: 283 529 543 467 23 335 246 1012
Cuts at: 3439 3525 3537 3883 1
Size: 86 12 346 449
Fragments arranged by size:
1012 543 529 467 449 346 335 283
246 86 23 12
BtgI C'CryG_G
Cuts at: 210 560 686 210
Size: 350 126 3855
Fragments arranged by size:
3855 350 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 252 328 1574 252
Size: 76 1246 3009
Fragments arranged by size:
3009 1246 76
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DraIII CAC_nnn'GTG
Cuts at: 542 542
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 234 592 951 2037 2895 234
Size: 358 359 1086 858 1670
Fragments arranged by size:
1670 1086 858 359 358
EaeI y'GGCC_r
Cuts at: 674 692 778 1916 2090 2481 2508 3853 674
Size: 18 86 1138 174 391 27 1345 1152
Fragments arranged by size:
1345 1152 1138 391 174 86 27 18
EagI C'GGCC_G
Cuts at: 674 778 1916 674
Size: 104 1138 3089
Fragments arranged by size:
3089 1138 104
EarI CTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 189 2539 2849 2995 4141 189
Size: 2350 310 146 1146 379
Fragments arranged by size:
2350 1146 379 310 146
EcoICRI GAG'CTC
Cuts at: 378 1164 1343 378
Size: 786 179 3366
Fragments arranged by size:
3366 786 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1021 1173 1294 2156 2588 3335 3603 388
Size: 633 152 121 862 432 747 268 1116
Fragments arranged by size:
1116 862 747 633 432 268 152 121
EcoO109I rG'GnC_Cy
Cuts at: 130 802 1032 1470 130
Size: 672 230 438 2991
Fragments arranged by size:
2991 672 438 230
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 975 2350 975
Size: 1375 2956
FspI TGC'GCA
Cuts at: 916 2112 916
Size: 1196 3135
FspAI rTGC'GCAy
Cuts at: 916 916
Size: 4331
HaeII r_GCGC'y
Cuts at: 435 450 622 1129 1765 2013 3035 4014 435
Size: 15 172 507 636 248 1022 979 752
Fragments arranged by size:
1022 979 752 636 507 248 172 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 483 515 2173 2203 2205 2235 483
Size: 32 1658 30 2 30 2579
Fragments arranged by size:
2579 1658 32 30 30 2
HincII GTy'rAC
Cuts at: 1115 1370 1115
Size: 255 4076
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 1193 3002 3186 353
Size: 840 1809 184 1498
Fragments arranged by size:
1809 1498 840 184
MscI TGG'CCA
Cuts at: 2092 2092
Size: 4331
MslI CAynn'nnrTG
Cuts at: 1102 2320 2447 1102
Size: 1218 127 2986
Fragments arranged by size:
2986 1218 127
MspA1I CmG'CkG
Cuts at: 212 458 670 679 688 1442 1756 2116 3129
Size: 246 212 9 9 754 314 360 1013
Cuts at: 3129 3374 212
Size: 245 1169
Fragments arranged by size:
1169 1013 754 360 314 246 245 212
9 9
NaeI GCC'GGC
Cuts at: 270 2512 270
Size: 2242 2089
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NgoMIV G'CCGG_C
Cuts at: 268 2510 268
Size: 2242 2089
NotI GC'GGCC_GC
Cuts at: 674 674
Size: 4331
NspI r_CATG'y
Cuts at: 333 915 1384 1884 2791 333
Size: 582 469 500 907 1873
Fragments arranged by size:
1873 907 582 500 469
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PmlI CAC'GTG
Cuts at: 533 542 533
Size: 9 4322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PpuMI rG'GwC_Cy
Cuts at: 1032 1032
Size: 4331
PshAI GACnn'nnGTC
Cuts at: 1246 1246
Size: 4331
PspOMI G'GGCC_C
Cuts at: 129 801 1149 129
Size: 672 348 3311
Fragments arranged by size:
3311 672 348
PstI C_TGCA'G
Cuts at: 629 684 1133 1378 2063 629
Size: 55 449 245 685 2897
Fragments arranged by size:
2897 685 449 245 55
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 291 2526 291
Size: 2235 2096
SacI G_AGCT'C
Cuts at: 380 1166 1345 380
Size: 786 179 3366
Fragments arranged by size:
3366 786 179
SacII CC_GC'GG
Cuts at: 213 689 213
Size: 476 3855
SalI G'TCGA_C
Cuts at: 1113 1368 1113
Size: 255 4076
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 426 625 680 1129 1374 2059 3052 3243
Size: 393 199 55 449 245 685 993 191
Cuts at: 3243 33
Size: 1121
Fragments arranged by size:
1121 993 685 449 393 245 199 191
55
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 800 1353 800
Size: 553 3778
SmlI C'TyrA_G
Cuts at: 340 1486 2401 2893 3155 340
Size: 1146 915 492 262 1516
Fragments arranged by size:
1516 1146 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 915 1384 1884 915
Size: 469 500 3362
Fragments arranged by size:
3362 500 469
SrfI GCCC'GGGC
Cuts at: 800 800
Size: 4331
StyI C'CwwG_G
Cuts at: 986 1465 1819 986
Size: 479 354 3498
Fragments arranged by size:
3498 479 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1914 2047 2506 2715 1914
Size: 133 459 209 3530
Fragments arranged by size:
3530 459 209 133
TatI w'GTAC_w
Cuts at: 3846 3846
Size: 4331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 307 339 3848 3880 307
Size: 32 3509 32 758
Fragments arranged by size:
3509 758 32 32
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XmaI C'CCGG_G
Cuts at: 798 1351 798
Size: 553 3778
ZraI GAC'GTC
Cuts at: 1244 1244
Size: 4331
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PciI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PasI PflMI PfoI PsiI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI XmnI