Restriction map for
pF1KB7677
(Circular) (Six-base) MAPSORT of: pF1KB7677.seq Check: 5621 from: 1 to: 4136
pF1KB7677 4136 bp
With 182 enzymes: SgfI *
October 16, 2009 16:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 590 590
Size: 4136
AatII G_ACGT'C
Cuts at: 422 422
Size: 4136
AccI GT'mk_AC
Cuts at: 1174 1174
Size: 4136
Acc65I G'GTAC_C
Cuts at: 1152 1152
Size: 4136
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 523 1961 2393 3140 3408 480
Size: 43 1438 432 747 268 1208
Fragments arranged by size:
1438 1208 747 432 268 43
AflIII A'CryG_T
Cuts at: 794 1488 2592 3569 794
Size: 694 1104 977 1361
Fragments arranged by size:
1361 1104 977 694
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AleI CACnn'nnGTG
Cuts at: 175 175
Size: 4136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 193 193
Size: 4136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 328
Size: 32 4104
AlwNI CAG_nnn'CTG
Cuts at: 899 1441 3008 899
Size: 542 1567 2027
Fragments arranged by size:
2027 1567 542
ApaI G_GGCC'C
Cuts at: 479 479
Size: 4136
ApaLI G'TGCA_C
Cuts at: 2906 2906
Size: 4136
ApoI r'AATT_y
Cuts at: 1140 3489 3745 1140
Size: 2349 256 1531
Fragments arranged by size:
2349 1531 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 1156 1156
Size: 4136
AvrII C'CTAG_G
Cuts at: 165 165
Size: 4136
BamHI G'GATC_C
Cuts at: 876 1161 876
Size: 285 3851
BanI G'GyrC_C
Cuts at: 169 395 1152 1566 1814 1849 169
Size: 226 757 414 248 35 2456
Fragments arranged by size:
2456 757 414 248 226 35
BanII G_rGCy'C
Cuts at: 465 479 701 830 890 1150 2180 465
Size: 14 222 129 60 260 1030 2421
Fragments arranged by size:
2421 1030 260 222 129 60 14
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BbsI GAAGACnn'nnnn_
Cuts at: 249 511 851 249
Size: 262 340 3534
Fragments arranged by size:
3534 340 262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4102
BciVI GTATCCnnnnn_n'
Cuts at: 2034 2795 4136 2034
Size: 761 1341 2034
Fragments arranged by size:
2034 1341 761
BclI T'GATC_A
Cuts at: 1656 1656
Size: 4136
BglII A'GATC_T
Cuts at: 860 1651 3688 860
Size: 791 2037 1308
Fragments arranged by size:
2037 1308 791
BlpI GC'TnA_GC
Cuts at: 1248 1248
Size: 4136
Bme1580I G_kGCm'C
Cuts at: 479 954 1761 1854 2910 479
Size: 475 807 93 1056 1705
Fragments arranged by size:
1705 1056 807 475 93
BmgBI CAC'GTC
Cuts at: 282 282
Size: 4136
BmrI ACTGGGnnnn_n'
Cuts at: 1348 1429 1764 3908 1348
Size: 81 335 2144 1576
Fragments arranged by size:
2144 1576 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 4136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 509 1084 1093 421
Size: 88 575 9 3464
Fragments arranged by size:
3464 575 88 9
Bpu10I CC'TnA_GC
Cuts at: 411 411
Size: 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 2191 2683 2981 1312
Size: 879 492 298 2467
Fragments arranged by size:
2467 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3840 3840
Size: 4136
BsaAI yAC'GTr
Cuts at: 177 795 1489 177
Size: 618 694 2824
Fragments arranged by size:
2824 694 618
BsaBI GATnn'nnATC
Cuts at: 6 1650 3687 6
Size: 1644 2037 455
Fragments arranged by size:
2037 1644 455
BsaHI Gr'CG_yC
Cuts at: 419 508 854 1567 1815 2517 4013 419
Size: 89 346 713 248 702 1496 542
Fragments arranged by size:
1496 713 702 542 346 248 89
BsaWI w'CCGG_w
Cuts at: 1128 1325 1846 2524 2798 2945 3356 1128
Size: 197 521 678 274 147 411 1908
Fragments arranged by size:
1908 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 328
Size: 30 4106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 682 682
Size: 4136
BseYI C'CCAG_C
Cuts at: 210 299 406 452 515 1561 2319 2896 3392
Size: 89 107 46 63 1046 758 577 496
Cuts at: 3392 210
Size: 954
Fragments arranged by size:
1046 954 758 577 496 107 89 63
46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 713 271
Size: 442 3694
BsiEI CG_ry'CG
Cuts at: 104 1678 1724 2932 104
Size: 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 341 701 1150 1928 2118 2910 341
Size: 360 449 778 190 792 1567
Fragments arranged by size:
1567 792 778 449 360 190
Bsp1286I G_dGCh'C
Cuts at: 341 465 479 701 830 890 954 1150 1761
Size: 124 14 222 129 60 64 196 611
Cuts at: 1761 1854 1928 2118 2180 2910 341
Size: 93 74 190 62 730 1567
Fragments arranged by size:
1567 730 611 222 196 190 129 124
93 74 64 62 60 14
BspEI T'CCGG_A
Cuts at: 3356 3356
Size: 4136
BspHI T'CATG_A
Cuts at: 3312 3312
Size: 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 215 590 1186 1400 1702 2083 215
Size: 375 596 214 302 381 2268
Fragments arranged by size:
2268 596 381 375 302 214
BsrBI CCG'CTC
Cuts at: 50 2428 2482 3966 4131 50
Size: 2378 54 1484 165 55
Fragments arranged by size:
2378 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2048 2048
Size: 4136
BsrFI r'CCGG_y
Cuts at: 2134 2315 2524 2134
Size: 181 209 3746
Fragments arranged by size:
3746 209 181
BssSI C'ACGA_G
Cuts at: 947 2407 2765 947
Size: 1460 358 2318
Fragments arranged by size:
2318 1460 358
BstAPI GCAn_nnn'nTGC
Cuts at: 294 1513 294
Size: 1219 2917
BstBI TT'CG_AA
Cuts at: 2497 2497
Size: 4136
BstYI r'GATC_y
Cuts at: 1 224 491 860 876 1161 1628 1651 1986
Size: 223 267 369 16 285 467 23 335
Cuts at: 1986 2232 3244 3330 3342 3688 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 369 346 335 285 267
246 223 86 23 16 12
BtgI C'CryG_G
Cuts at: 332 332
Size: 4136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 751 2086 2267 2430 751
Size: 1335 181 163 2457
Fragments arranged by size:
2457 1335 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 1379
Size: 4136
DraI TTT'AAA
Cuts at: 1135 1135
Size: 4136
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 621 1721 1895 2286 2313 3658 621
Size: 1100 174 391 27 1345 1099
Fragments arranged by size:
1345 1100 1099 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 533 2159 2369 533
Size: 1626 210 2300
Fragments arranged by size:
2300 1626 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2344 2654 2800 3946 2344
Size: 310 146 1146 2534
Fragments arranged by size:
2534 1146 310 146
EcoICRI GAG'CTC
Cuts at: 699 1148 699
Size: 449 3687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 480 509 523 1084 1093 1961 2393 3140
Size: 59 29 14 561 9 868 432 747
Cuts at: 3140 3408 421
Size: 268 1149
Fragments arranged by size:
1149 868 747 561 432 268 59 29
14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 164 379 164
Size: 215 3921
EcoO109I rG'GnC_Cy
Cuts at: 476 710 747 1275 476
Size: 234 37 528 3337
Fragments arranged by size:
3337 528 234 37
EcoRI G'AATT_C
Cuts at: 1140 1140
Size: 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 405 2155 405
Size: 1750 2386
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 1570 1818 2840 3819 1570
Size: 248 1022 979 1887
Fragments arranged by size:
1887 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 236 268 350 382 928 960 1978 2008 2010
Size: 32 82 32 546 32 1018 30 2
Cuts at: 2010 2040 236
Size: 30 2332
Fragments arranged by size:
2332 1018 546 82 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1175 1175
Size: 4136
HindIII A'AGCT_T
Cuts at: 970 970
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 1156 1156
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2807 2991 2807
Size: 184 3952
MscI TGG'CCA
Cuts at: 1897 1897
Size: 4136
MslI CAynn'nnrTG
Cuts at: 175 629 2125 2252 175
Size: 454 1496 127 2059
Fragments arranged by size:
2059 1496 454 127
MspA1I CmG'CkG
Cuts at: 334 456 804 1071 1247 1561 1921 2934 3179
Size: 122 348 267 176 314 360 1013 245
Cuts at: 3179 334
Size: 1291
Fragments arranged by size:
1291 1013 360 348 314 267 245 176
122
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NspI r_CATG'y
Cuts at: 1189 1689 2596 1189
Size: 500 907 2729
Fragments arranged by size:
2729 907 500
PasI CC'CwG_GG
Cuts at: 601 714 601
Size: 113 4023
PciI A'CATG_T
Cuts at: 2592 2592
Size: 4136
PflMI CCAn_nnn'nTGG
Cuts at: 899 899
Size: 4136
PfoI T'CCnGG_A
Cuts at: 1029 1043 1029
Size: 14 4122
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PmlI CAC'GTG
Cuts at: 177 177
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 328 360 3275 3307 328
Size: 32 2915 32 1157
Fragments arranged by size:
2915 1157 32 32
PpuMI rG'GwC_Cy
Cuts at: 710 710
Size: 4136
PsiI TTA'TAA
Cuts at: 968 968
Size: 4136
PspOMI G'GGCC_C
Cuts at: 475 475
Size: 4136
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 916 948 916
Size: 32 4104
PstI C_TGCA'G
Cuts at: 590 709 1183 1868 590
Size: 119 474 685 2858
Fragments arranged by size:
2858 685 474 119
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 804 1561 1921 804
Size: 757 360 3019
Fragments arranged by size:
3019 757 360
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 701 1150 701
Size: 449 3687
SacII CC_GC'GG
Cuts at: 335 335
Size: 4136
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 316 3653 316
Size: 3337 799
SfcI C'TryA_G
Cuts at: 33 586 705 1179 1864 2857 3048 33
Size: 553 119 474 685 993 191 1121
Fragments arranged by size:
1121 993 685 553 474 191 119
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 1158 1158
Size: 4136
SmlI C'TyrA_G
Cuts at: 1291 2206 2698 2960 1291
Size: 915 492 262 2467
Fragments arranged by size:
2467 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 1189 1689 1189
Size: 500 3636
StyI C'CwwG_G
Cuts at: 165 380 1270 1624 165
Size: 215 890 354 2677
Fragments arranged by size:
2677 890 354 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 187 202 529 1719 1852 2311 2520 187
Size: 15 327 1190 133 459 209 1803
Fragments arranged by size:
1803 1190 459 327 209 133 15
TatI w'GTAC_w
Cuts at: 314 440 3651 314
Size: 126 3211 799
Fragments arranged by size:
3211 799 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4104
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1631 1631
Size: 4136
XmaI C'CCGG_G
Cuts at: 1156 1156
Size: 4136
ZraI GAC'GTC
Cuts at: 420 420
Size: 4136
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI BaeI BbvCI BfrBI BglI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PshAI PspXI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI