Restriction map for
pF1KB7680
(Circular) (Six-base) MAPSORT of: pF1KB7680.seq Check: 2165 from: 1 to: 4331
pF1KB7680 4331 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 2156 2588 3335 3603 292
Size: 1864 432 747 268 1020
Fragments arranged by size:
1864 1020 747 432 268
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 311 700 1636 3203 311
Size: 389 936 1567 1439
Fragments arranged by size:
1567 1439 936 389
ApaI G_GGCC'C
Cuts at: 567 844 864 567
Size: 277 20 4034
Fragments arranged by size:
4034 277 20
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 1267 1335 3684 3940 1267
Size: 68 2349 256 1658
Fragments arranged by size:
2349 1658 256 68
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 634 1021 1351 634
Size: 387 330 3614
Fragments arranged by size:
3614 387 330
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 577 610 577
Size: 33 4298
BamHI G'GATC_C
Cuts at: 1317 1356 1317
Size: 39 4292
BanI G'GyrC_C
Cuts at: 784 1072 1120 1347 1761 2009 2044 784
Size: 288 48 227 414 248 35 3071
Fragments arranged by size:
3071 414 288 248 227 48 35
BanII G_rGCy'C
Cuts at: 523 567 635 670 844 864 899 1345 2375
Size: 44 68 35 174 20 35 446 1030
Cuts at: 2375 523
Size: 2479
Fragments arranged by size:
2479 1030 446 174 68 44 35 35
20
BbeI G_GCGC'C
Cuts at: 1076 1765 2013 1076
Size: 689 248 3394
Fragments arranged by size:
3394 689 248
BbsI GAAGACnn'nnnn_
Cuts at: 803 967 803
Size: 164 4167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1002 1036 1002
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 1059 1169 1059
Size: 110 4221
BglII A'GATC_T
Cuts at: 434 1846 3883 434
Size: 1412 2037 882
Fragments arranged by size:
2037 1412 882
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 253 567 844 864 1956 2049 3105 253
Size: 314 277 20 1092 93 1056 1479
Fragments arranged by size:
1479 1092 1056 314 277 93 20
BmrI ACTGGGnnnn_n'
Cuts at: 977 1543 1624 1959 4103 977
Size: 566 81 335 2144 1205
Fragments arranged by size:
2144 1205 566 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 1085 1186 1229 1270 1312 507
Size: 578 101 43 41 42 3526
Fragments arranged by size:
3526 578 101 43 42 41
Bpu10I CC'TnA_GC
Cuts at: 490 490
Size: 4331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4035 4035
Size: 4331
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 115 1073 1172 1762 2010 2712 4208 115
Size: 958 99 590 248 702 1496 238
Fragments arranged by size:
1496 958 702 590 248 238 99
BsaWI w'CCGG_w
Cuts at: 173 1084 1520 2041 2719 2993 3140 3551 173
Size: 911 436 521 678 274 147 411 953
Fragments arranged by size:
953 911 678 521 436 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 647 677 976 1006 647
Size: 30 299 30 3972
Fragments arranged by size:
3972 299 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 1142 1159 547
Size: 595 17 3719
Fragments arranged by size:
3719 595 17
BseYI C'CCAG_C
Cuts at: 566 837 865 1159 1756 2514 3091 3587 566
Size: 271 28 294 597 758 577 496 1310
Fragments arranged by size:
1310 758 597 577 496 294 271 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 484 154
Size: 330 4001
BsiEI CG_ry'CG
Cuts at: 104 583 1873 1919 3127 104
Size: 479 1290 46 1208 1308
Fragments arranged by size:
1308 1290 1208 479 46
BsiHKAI G_wGCw'C
Cuts at: 523 670 899 1345 2123 2313 3105 523
Size: 147 229 446 778 190 792 1749
Fragments arranged by size:
1749 792 778 446 229 190 147
BsmI GAATG_Cn'
Cuts at: 129 138 234 978 129
Size: 9 96 744 3482
Fragments arranged by size:
3482 744 96 9
Bsp1286I G_dGCh'C
Cuts at: 253 523 567 635 670 844 864 899 1345
Size: 270 44 68 35 174 20 35 446
Cuts at: 1345 1956 2049 2123 2313 2375 3105 253
Size: 611 93 74 190 62 730 1479
Fragments arranged by size:
1479 730 611 446 270 190 174 93
74 68 62 44 35 35 20
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 373 1381 1595 1897 2278 373
Size: 1008 214 302 381 2426
Fragments arranged by size:
2426 1008 381 302 214
BsrBI CCG'CTC
Cuts at: 50 344 1026 2623 2677 4161 4326 50
Size: 294 682 1597 54 1484 165 55
Fragments arranged by size:
1597 1484 682 294 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1013 2243 1013
Size: 1230 3101
BsrFI r'CCGG_y
Cuts at: 117 787 2329 2510 2719 117
Size: 670 1542 181 209 1729
Fragments arranged by size:
1729 1542 670 209 181
BssSI C'ACGA_G
Cuts at: 898 922 2602 2960 898
Size: 24 1680 358 2269
Fragments arranged by size:
2269 1680 358 24
BstAPI GCAn_nnn'nTGC
Cuts at: 555 1708 555
Size: 1153 3178
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstEII G'GTnAC_C
Cuts at: 805 805
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 1002 1002
Size: 4331
BstYI r'GATC_y
Cuts at: 1 434 1317 1356 1823 1846 2181 2427 3439
Size: 433 883 39 467 23 335 246 1012
Cuts at: 3439 3525 3537 3883 1
Size: 86 12 346 449
Fragments arranged by size:
1012 883 467 449 433 346 335 246
86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 1142 1142
Size: 4331
BtgI C'CryG_G
Cuts at: 717 717
Size: 4331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1185 2281 2462 2625 1185
Size: 1096 181 163 2891
Fragments arranged by size:
2891 1096 181 163
BtsI GCAGTG_nn'
Cuts at: 229 747 1574 229
Size: 518 827 2986
Fragments arranged by size:
2986 827 518
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 325 2037 2895 325
Size: 1712 858 1761
Fragments arranged by size:
1761 1712 858
EaeI y'GGCC_r
Cuts at: 400 580 1285 1916 2090 2481 2508 3853 400
Size: 180 705 631 174 391 27 1345 878
Fragments arranged by size:
1345 878 705 631 391 180 174 27
EagI C'GGCC_G
Cuts at: 580 1916 580
Size: 1336 2995
EarI CTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 475 666 2539 2849 2995 4141 475
Size: 191 1873 310 146 1146 665
Fragments arranged by size:
1873 1146 665 310 191 146
EcoICRI GAG'CTC
Cuts at: 521 668 897 1343 521
Size: 147 229 446 3509
Fragments arranged by size:
3509 446 229 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 507 1085 1186 1229 1270 1312 2156 2588
Size: 215 578 101 43 41 42 844 432
Cuts at: 2588 3335 3603 292
Size: 747 268 1020
Fragments arranged by size:
1020 844 747 578 432 268 215 101
43 42 41
EcoO109I rG'GnC_Cy
Cuts at: 563 697 841 860 861 1060 1090 1470 563
Size: 134 144 19 1 199 30 380 3424
Fragments arranged by size:
3424 380 199 144 134 30 19 1
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 1012 2112 1012
Size: 1100 3231
HaeII r_GCGC'y
Cuts at: 1076 1765 2013 3035 4014 1076
Size: 689 248 1022 979 1393
Fragments arranged by size:
1393 1022 979 689 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 467 499 652 684 976 1008 2173 2203 2205
Size: 32 153 32 292 32 1165 30 2
Cuts at: 2205 2235 467
Size: 30 2563
Fragments arranged by size:
2563 1165 292 153 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
KasI G'GCGC_C
Cuts at: 1072 1761 2009 1072
Size: 689 248 3394
Fragments arranged by size:
3394 689 248
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 2092 2092
Size: 4331
MslI CAynn'nnrTG
Cuts at: 610 2320 2447 610
Size: 1710 127 2494
Fragments arranged by size:
2494 1710 127
MspA1I CmG'CkG
Cuts at: 1442 1756 2116 3129 3374 1442
Size: 314 360 1013 245 2399
Fragments arranged by size:
2399 1013 360 314 245
NaeI GCC'GGC
Cuts at: 119 2512 119
Size: 2393 1938
NarI GG'CG_CC
Cuts at: 1073 1762 2010 1073
Size: 689 248 3394
Fragments arranged by size:
3394 689 248
NgoMIV G'CCGG_C
Cuts at: 117 2510 117
Size: 2393 1938
NspI r_CATG'y
Cuts at: 270 549 1384 1884 2791 270
Size: 279 835 500 907 1810
Fragments arranged by size:
1810 907 835 500 279
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 886 918 3470 3502 886
Size: 32 2552 32 1715
Fragments arranged by size:
2552 1715 32 32
PpuMI rG'GwC_Cy
Cuts at: 1090 1090
Size: 4331
PspOMI G'GGCC_C
Cuts at: 563 840 860 563
Size: 277 20 4034
Fragments arranged by size:
4034 277 20
PspXI vC'TCGA_Gb
Cuts at: 1021 1021
Size: 4331
PstI C_TGCA'G
Cuts at: 1378 2063 1378
Size: 685 3646
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 523 670 899 1345 523
Size: 147 229 446 3509
Fragments arranged by size:
3509 446 229 147
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 1000 3848 1000
Size: 2848 1483
SfcI C'TryA_G
Cuts at: 33 1374 2059 3052 3243 33
Size: 1341 685 993 191 1121
Fragments arranged by size:
1341 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1074 1763 2011 1074
Size: 689 248 3394
Fragments arranged by size:
3394 689 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SgrAI Cr'CCGG_yG
Cuts at: 117 117
Size: 4331
SmaI CCC'GGG
Cuts at: 636 1353 636
Size: 717 3614
SmlI C'TyrA_G
Cuts at: 1021 1486 2401 2893 3155 1021
Size: 465 915 492 262 2197
Fragments arranged by size:
2197 915 492 465 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 549 1384 1884 549
Size: 835 500 2996
Fragments arranged by size:
2996 835 500
StyI C'CwwG_G
Cuts at: 290 1313 1465 1819 290
Size: 1023 152 354 2802
Fragments arranged by size:
2802 1023 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 215 488 766 1088 1914 2047 2506 2715 215
Size: 273 278 322 826 133 459 209 1831
Fragments arranged by size:
1831 826 459 322 278 273 209 133
TatI w'GTAC_w
Cuts at: 998 1187 3846 998
Size: 189 2659 1483
Fragments arranged by size:
2659 1483 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 647 679 1302 1334 3848 3880 647
Size: 32 623 32 2514 32 1098
Fragments arranged by size:
2514 1098 623 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XhoI C'TCGA_G
Cuts at: 1021 1021
Size: 4331
XmaI C'CCGG_G
Cuts at: 634 1351 634
Size: 717 3614
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BfrBI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PshAI PsiI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XmnI ZraI