Restriction map for
pF1KB7683
(Circular) (Six-base) MAPSORT of: pF1KB7683.seq Check: 6883 from: 1 to: 4151
pF1KB7683 4151 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 341 341
Size: 4151
AccI GT'mk_AC
Cuts at: 1025 1189 1025
Size: 164 3987
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1976 2408 3155 3423 935
Size: 1041 432 747 268 1663
Fragments arranged by size:
1663 1041 747 432 268
AfeI AGC'GCT
Cuts at: 925 925
Size: 4151
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 412 1456 3023 412
Size: 1044 1567 1540
Fragments arranged by size:
1567 1540 1044
ApaI G_GGCC'C
Cuts at: 298 298
Size: 4151
ApaLI G'TGCA_C
Cuts at: 790 2921 790
Size: 2131 2020
ApoI r'AATT_y
Cuts at: 213 1155 3504 3760 213
Size: 942 2349 256 604
Fragments arranged by size:
2349 942 604 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 1171 1171
Size: 4151
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 1167 1581 1829 1864 1167
Size: 414 248 35 3454
Fragments arranged by size:
3454 414 248 35
BanII G_rGCy'C
Cuts at: 298 899 1165 2195 298
Size: 601 266 1030 2254
Fragments arranged by size:
2254 1030 601 266
BbeI G_GCGC'C
Cuts at: 1585 1833 1585
Size: 248 3903
BbsI GAAGACnn'nnnn_
Cuts at: 1052 1052
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglII A'GATC_T
Cuts at: 319 1666 3703 319
Size: 1347 2037 767
Fragments arranged by size:
2037 1347 767
BlpI GC'TnA_GC
Cuts at: 1263 1263
Size: 4151
Bme1580I G_kGCm'C
Cuts at: 298 794 1776 1869 2925 298
Size: 496 982 93 1056 1524
Fragments arranged by size:
1524 1056 982 496 93
BmgBI CAC'GTC
Cuts at: 780 780
Size: 4151
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1099 2518 1099
Size: 1419 2732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 1069 1327 2206 2698 2996 249
Size: 820 258 879 492 298 1404
Fragments arranged by size:
1404 879 820 492 298 258
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 338 1582 1830 2532 4028 338
Size: 1244 248 702 1496 461
Fragments arranged by size:
1496 1244 702 461 248
BsaWI w'CCGG_w
Cuts at: 118 1340 1861 2539 2813 2960 3371 118
Size: 1222 521 678 274 147 411 898
Fragments arranged by size:
1222 898 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1576 2334 2911 3407 1576
Size: 758 577 496 2320
Fragments arranged by size:
2320 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1693 1739 2947 104
Size: 1589 46 1208 1308
Fragments arranged by size:
1589 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 794 1165 1943 2133 2925 794
Size: 371 778 190 792 2020
Fragments arranged by size:
2020 792 778 371 190
BsmBI CGTCTCn'nnnn_
Cuts at: 145 145
Size: 4151
Bsp1286I G_dGCh'C
Cuts at: 298 794 899 1165 1776 1869 1943 2133 2195
Size: 496 105 266 611 93 74 190 62
Cuts at: 2195 2925 298
Size: 730 1524
Fragments arranged by size:
1524 730 611 496 266 190 105 93
74 62
BspEI T'CCGG_A
Cuts at: 118 3371 118
Size: 3253 898
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1201 1415 1717 2098 1201
Size: 214 302 381 3254
Fragments arranged by size:
3254 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2443 2497 3981 4146 50
Size: 2393 54 1484 165 55
Fragments arranged by size:
2393 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 726 2063 726
Size: 1337 2814
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BsrGI T'GTAC_A
Cuts at: 513 513
Size: 4151
BssSI C'ACGA_G
Cuts at: 2422 2780 2422
Size: 358 3793
BstAPI GCAn_nnn'nTGC
Cuts at: 1528 1528
Size: 4151
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstYI r'GATC_y
Cuts at: 1 319 1176 1643 1666 2001 2247 3259 3345
Size: 318 857 467 23 335 246 1012 86
Cuts at: 3345 3357 3703 1
Size: 12 346 449
Fragments arranged by size:
1012 857 467 449 346 335 318 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1026 1026
Size: 4151
BtgI C'CryG_G
Cuts at: 107 107
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 1394
Size: 4151
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 1736 1910 2301 2328 3673 1736
Size: 174 391 27 1345 2214
Fragments arranged by size:
2214 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1736 1736
Size: 4151
EarI CTCTTCn'nnn_
Cuts at: 980 1112 2174 2384 980
Size: 132 1062 210 2747
Fragments arranged by size:
2747 1062 210 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2359 2669 2815 3961 2359
Size: 310 146 1146 2549
Fragments arranged by size:
2549 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 935 1976 2408 3155 3423 935
Size: 1041 432 747 268 1663
Fragments arranged by size:
1663 1041 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 302 302
Size: 4151
EcoO109I rG'GnC_Cy
Cuts at: 295 1290 295
Size: 995 3156
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2170 2170
Size: 4151
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 927 1585 1833 2855 3834 927
Size: 658 248 1022 979 1244
Fragments arranged by size:
1244 1022 979 658 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1993 2023 2025 2055 1993
Size: 30 2 30 4089
Fragments arranged by size:
4089 30 30 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
HindIII A'AGCT_T
Cuts at: 752 752
Size: 4151
KasI G'GCGC_C
Cuts at: 1581 1829 1581
Size: 248 3903
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3006 2822
Size: 184 3967
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 437 2140 2267 437
Size: 1703 127 2321
Fragments arranged by size:
2321 1703 127
MspA1I CmG'CkG
Cuts at: 1262 1576 1936 2949 3194 1262
Size: 314 360 1013 245 2219
Fragments arranged by size:
2219 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 1582 1830 1582
Size: 248 3903
NcoI C'CATG_G
Cuts at: 107 107
Size: 4151
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 1204 1704 2611 1204
Size: 500 907 2744
Fragments arranged by size:
2744 907 500
PasI CC'CwG_GG
Cuts at: 298 491 298
Size: 193 3958
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PfoI T'CCnGG_A
Cuts at: 221 381 744 221
Size: 160 363 3628
Fragments arranged by size:
3628 363 160
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PspOMI G'GGCC_C
Cuts at: 294 294
Size: 4151
PstI C_TGCA'G
Cuts at: 1198 1883 1198
Size: 685 3466
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 1576 1936 1576
Size: 360 3791
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SfcI C'TryA_G
Cuts at: 33 585 1194 1879 2872 3063 33
Size: 552 609 685 993 191 1121
Fragments arranged by size:
1121 993 685 609 552 191
SfoI GGC'GCC
Cuts at: 1583 1831 1583
Size: 248 3903
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 1173 1173
Size: 4151
SmlI C'TyrA_G
Cuts at: 228 1084 1306 2221 2713 2975 228
Size: 856 222 915 492 262 1404
Fragments arranged by size:
1404 915 856 492 262 222
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 1204 1704 1204
Size: 500 3651
SspI AAT'ATT
Cuts at: 579 579
Size: 4151
StyI C'CwwG_G
Cuts at: 107 1285 1639 107
Size: 1178 354 2619
Fragments arranged by size:
2619 1178 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1734 1867 2326 2535 1734
Size: 133 459 209 3350
Fragments arranged by size:
3350 459 209 133
TatI w'GTAC_w
Cuts at: 513 3666 513
Size: 3153 998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 200 232 3668 3700 200
Size: 32 3436 32 651
Fragments arranged by size:
3436 651 32 32
Tth111I GACn'n_nGTC
Cuts at: 1948 1948
Size: 4151
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 771 1646 771
Size: 875 3276
XmaI C'CCGG_G
Cuts at: 1171 1171
Size: 4151
ZraI GAC'GTC
Cuts at: 339 339
Size: 4151
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmtI BpmI Bpu10I
BsaXI BseRI BsgI BsiWI BsmI BssHII BstEII BstXI
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI