Restriction map for
pF1KB7685
(Circular) (Six-base) MAPSORT of: pF1KB7685.seq Check: 4294 from: 1 to: 4157
pF1KB7685 4157 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1195 1195
Size: 4157
Acc65I G'GTAC_C
Cuts at: 1173 1173
Size: 4157
AclI AA'CG_TT
Cuts at: 3997 3997
Size: 4157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 993 1982 2414 3161 3429 222
Size: 771 989 432 747 268 950
Fragments arranged by size:
989 950 771 747 432 268
AfeI AGC'GCT
Cuts at: 934 934
Size: 4157
AflIII A'CryG_T
Cuts at: 1509 2613 3590 1509
Size: 1104 977 2076
Fragments arranged by size:
2076 1104 977
AgeI A'CCGG_T
Cuts at: 2545 2545
Size: 4157
AleI CACnn'nnGTG
Cuts at: 198 1052 198
Size: 854 3303
AlwNI CAG_nnn'CTG
Cuts at: 315 375 430 1462 3029 315
Size: 60 55 1032 1567 1443
Fragments arranged by size:
1567 1443 1032 60 55
ApaLI G'TGCA_C
Cuts at: 413 2927 413
Size: 2514 1643
ApoI r'AATT_y
Cuts at: 697 1127 1161 3510 3766 697
Size: 430 34 2349 256 1088
Fragments arranged by size:
2349 1088 430 256 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 4157
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
AvaI C'yCGr_G
Cuts at: 1177 1177
Size: 4157
BamHI G'GATC_C
Cuts at: 1182 1182
Size: 4157
BanI G'GyrC_C
Cuts at: 1173 1587 1835 1870 1173
Size: 414 248 35 3460
Fragments arranged by size:
3460 414 248 35
BanII G_rGCy'C
Cuts at: 302 1171 2201 302
Size: 869 1030 2258
Fragments arranged by size:
2258 1030 869
BbeI G_GCGC'C
Cuts at: 1591 1839 1591
Size: 248 3909
BbsI GAAGACnn'nnnn_
Cuts at: 813 813
Size: 4157
BbvCI CC'TCA_GC
Cuts at: 316 740 316
Size: 424 3733
BciVI GTATCCnnnnn_n'
Cuts at: 2055 2816 4157 2055
Size: 761 1341 2055
Fragments arranged by size:
2055 1341 761
BclI T'GATC_A
Cuts at: 1677 1677
Size: 4157
BglII A'GATC_T
Cuts at: 1672 3709 1672
Size: 2037 2120
BlpI GC'TnA_GC
Cuts at: 1269 1269
Size: 4157
Bme1580I G_kGCm'C
Cuts at: 417 1782 1875 2931 417
Size: 1365 93 1056 1643
Fragments arranged by size:
1643 1365 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 183 1369 1450 1785 3929 183
Size: 1186 81 335 2144 411
Fragments arranged by size:
2144 1186 411 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 4157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 452 475 484 622 452
Size: 23 9 138 3987
Fragments arranged by size:
3987 138 23 9
Bpu10I CC'TnA_GC
Cuts at: 316 376 740 316
Size: 60 364 3733
Fragments arranged by size:
3733 364 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1333 2212 2704 3002 1333
Size: 879 492 298 2488
Fragments arranged by size:
2488 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3861 3861
Size: 4157
BsaAI yAC'GTr
Cuts at: 1510 1510
Size: 4157
BsaBI GATnn'nnATC
Cuts at: 6 1671 3708 6
Size: 1665 2037 455
Fragments arranged by size:
2037 1665 455
BsaHI Gr'CG_yC
Cuts at: 1588 1836 2538 4034 1588
Size: 248 702 1496 1711
Fragments arranged by size:
1711 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1346 1867 2545 2819 2966 3377 1346
Size: 521 678 274 147 411 2126
Fragments arranged by size:
2126 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 728 451
Size: 277 3880
BseYI C'CCAG_C
Cuts at: 287 596 613 1582 2340 2917 3413 287
Size: 309 17 969 758 577 496 1031
Fragments arranged by size:
1031 969 758 577 496 309 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 222
Size: 4157
BsiEI CG_ry'CG
Cuts at: 104 1699 1745 2953 104
Size: 1595 46 1208 1308
Fragments arranged by size:
1595 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 302 417 1171 1949 2139 2931 302
Size: 115 754 778 190 792 1528
Fragments arranged by size:
1528 792 778 754 190 115
BsmI GAATG_Cn'
Cuts at: 331 380 331
Size: 49 4108
Bsp1286I G_dGCh'C
Cuts at: 302 417 1171 1782 1875 1949 2139 2201 2931
Size: 115 754 611 93 74 190 62 730
Cuts at: 2931 302
Size: 1528
Fragments arranged by size:
1528 754 730 611 190 115 93 74
62
BspEI T'CCGG_A
Cuts at: 3377 3377
Size: 4157
BspHI T'CATG_A
Cuts at: 3333 3333
Size: 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 404 483 1207 1421 1723 2104 362
Size: 42 79 724 214 302 381 2415
Fragments arranged by size:
2415 724 381 302 214 79 42
BsrBI CCG'CTC
Cuts at: 50 2449 2503 3987 4152 50
Size: 2399 54 1484 165 55
Fragments arranged by size:
2399 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2069 2069
Size: 4157
BsrFI r'CCGG_y
Cuts at: 2155 2336 2545 2155
Size: 181 209 3767
Fragments arranged by size:
3767 209 181
BssSI C'ACGA_G
Cuts at: 2428 2786 2428
Size: 358 3799
BstAPI GCAn_nnn'nTGC
Cuts at: 1534 1534
Size: 4157
BstBI TT'CG_AA
Cuts at: 2518 2518
Size: 4157
BstXI CCAn_nnnn'nTGG
Cuts at: 120 120
Size: 4157
BstYI r'GATC_y
Cuts at: 1 459 1182 1649 1672 2007 2253 3265 3351
Size: 458 723 467 23 335 246 1012 86
Cuts at: 3351 3363 3709 1
Size: 12 346 449
Fragments arranged by size:
1012 723 467 458 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 128 128
Size: 4157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2107 2288 2451 2107
Size: 181 163 3813
Fragments arranged by size:
3813 181 163
BtsI GCAGTG_nn'
Cuts at: 449 1400 449
Size: 951 3206
DraI TTT'AAA
Cuts at: 1156 1156
Size: 4157
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2721 1863
Size: 858 3299
EaeI y'GGCC_r
Cuts at: 1742 1916 2307 2334 3679 1742
Size: 174 391 27 1345 2220
Fragments arranged by size:
2220 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1742 1742
Size: 4157
EarI CTCTTCn'nnn_
Cuts at: 653 2180 2390 653
Size: 1527 210 2420
Fragments arranged by size:
2420 1527 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2365 2675 2821 3967 2365
Size: 310 146 1146 2555
Fragments arranged by size:
2555 1146 310 146
EcoICRI GAG'CTC
Cuts at: 300 1169 300
Size: 869 3288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 452 475 484 622 993 1982 2414 3161
Size: 230 23 9 138 371 989 432 747
Cuts at: 3161 3429 222
Size: 268 950
Fragments arranged by size:
989 950 747 432 371 268 230 138
23 9
EcoNI CCTnn'n_nnAGG
Cuts at: 549 549
Size: 4157
EcoO109I rG'GnC_Cy
Cuts at: 495 1296 495
Size: 801 3356
EcoRI G'AATT_C
Cuts at: 697 1127 1161 697
Size: 430 34 3693
Fragments arranged by size:
3693 430 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 4157
FspI TGC'GCA
Cuts at: 1938 1938
Size: 4157
HaeII r_GCGC'y
Cuts at: 936 1591 1839 2861 3840 936
Size: 655 248 1022 979 1253
Fragments arranged by size:
1253 1022 979 655 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 175 207 766 798 1999 2029 2031 2061 175
Size: 32 559 32 1201 30 2 30 2271
Fragments arranged by size:
2271 1201 559 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1196 1196
Size: 4157
KasI G'GCGC_C
Cuts at: 1587 1835 1587
Size: 248 3909
KpnI G_GTAC'C
Cuts at: 1177 1177
Size: 4157
MluI A'CGCG_T
Cuts at: 3590 3590
Size: 4157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2828 3012 2828
Size: 184 3973
MscI TGG'CCA
Cuts at: 1918 1918
Size: 4157
MslI CAynn'nnrTG
Cuts at: 198 526 1052 2146 2273 198
Size: 328 526 1094 127 2082
Fragments arranged by size:
2082 1094 526 328 127
MspA1I CmG'CkG
Cuts at: 291 600 1268 1582 1942 2955 3200 291
Size: 309 668 314 360 1013 245 1248
Fragments arranged by size:
1248 1013 668 360 314 309 245
NaeI GCC'GGC
Cuts at: 2338 2338
Size: 4157
NarI GG'CG_CC
Cuts at: 1588 1836 1588
Size: 248 3909
NdeI CA'TA_TG
Cuts at: 558 558
Size: 4157
NgoMIV G'CCGG_C
Cuts at: 2336 2336
Size: 4157
NspI r_CATG'y
Cuts at: 1210 1710 2617 1210
Size: 500 907 2750
Fragments arranged by size:
2750 907 500
PciI A'CATG_T
Cuts at: 2613 2613
Size: 4157
PflMI CCAn_nnn'nTGG
Cuts at: 430 430
Size: 4157
PmeI GTTT'AAAC
Cuts at: 1156 1156
Size: 4157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 4125
PpuMI rG'GwC_Cy
Cuts at: 495 495
Size: 4157
PstI C_TGCA'G
Cuts at: 1204 1889 1204
Size: 685 3472
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4157
PvuII CAG'CTG
Cuts at: 291 600 1582 1942 291
Size: 309 982 360 2506
Fragments arranged by size:
2506 982 360 309
RsrII CG'GwC_CG
Cuts at: 2352 2352
Size: 4157
SacI G_AGCT'C
Cuts at: 302 1171 302
Size: 869 3288
SalI G'TCGA_C
Cuts at: 1194 1194
Size: 4157
SapI GCTCTTCn'nnn_
Cuts at: 2180 2390 2180
Size: 210 3947
SbfI CC_TGCA'GG
Cuts at: 1204 1204
Size: 4157
ScaI AGT'ACT
Cuts at: 3674 3674
Size: 4157
SfcI C'TryA_G
Cuts at: 33 225 1200 1885 2878 3069 33
Size: 192 975 685 993 191 1121
Fragments arranged by size:
1121 993 975 685 192 191
SfoI GGC'GCC
Cuts at: 1589 1837 1589
Size: 248 3909
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4157
SmaI CCC'GGG
Cuts at: 1179 1179
Size: 4157
SmlI C'TyrA_G
Cuts at: 1312 2227 2719 2981 1312
Size: 915 492 262 2488
Fragments arranged by size:
2488 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4157
SphI G_CATG'C
Cuts at: 1210 1710 1210
Size: 500 3657
StuI AGG'CCT
Cuts at: 1089 1089
Size: 4157
StyI C'CwwG_G
Cuts at: 335 545 702 1291 1645 335
Size: 210 157 589 354 2847
Fragments arranged by size:
2847 589 354 210 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 396 1740 1873 2332 2541 396
Size: 1344 133 459 209 2012
Fragments arranged by size:
2012 1344 459 209 133
TatI w'GTAC_w
Cuts at: 960 3672 960
Size: 2712 1445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 557 589 3674 3706 557
Size: 32 3085 32 1008
Fragments arranged by size:
3085 1008 32 32
Tth111I GACn'n_nGTC
Cuts at: 1954 1954
Size: 4157
XbaI T'CTAG_A
Cuts at: 1188 1188
Size: 4157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 427 1652 427
Size: 1225 2932
XmaI C'CCGG_G
Cuts at: 1177 1177
Size: 4157
XmnI GAAnn'nnTTC
Cuts at: 838 1006 838
Size: 168 3989
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI