Restriction map for
pF1KB7689
(Circular) (Six-base) MAPSORT of: pF1KB7689.seq Check: 5500 from: 1 to: 4160
pF1KB7689 4160 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 431 1198 431
Size: 767 3393
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 1985 2417 3164 3432 408
Size: 1577 432 747 268 1136
Fragments arranged by size:
1577 1136 747 432 268
AflIII A'CryG_T
Cuts at: 1512 2616 3593 1512
Size: 1104 977 2079
Fragments arranged by size:
2079 1104 977
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1018 1018
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 1019 1465 3032 1019
Size: 446 1567 2147
Fragments arranged by size:
2147 1567 446
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 345 363 1164 3513 3769 345
Size: 18 801 2349 256 736
Fragments arranged by size:
2349 801 736 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4160
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 171 911 1097 1180 171
Size: 740 186 83 3151
Fragments arranged by size:
3151 740 186 83
BamHI G'GATC_C
Cuts at: 1185 1185
Size: 4160
BanI G'GyrC_C
Cuts at: 1002 1176 1590 1838 1873 1002
Size: 174 414 248 35 3289
Fragments arranged by size:
3289 414 248 174 35
BanII G_rGCy'C
Cuts at: 179 764 1174 2204 179
Size: 585 410 1030 2135
Fragments arranged by size:
2135 1030 585 410
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 503 782 503
Size: 279 3881
BbvCI CC'TCA_GC
Cuts at: 895 895
Size: 4160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4126
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BfrBI ATG'CAT
Cuts at: 225 225
Size: 4160
BglI GCCn_nnn'nGGC
Cuts at: 861 861
Size: 4160
BglII A'GATC_T
Cuts at: 776 1675 3712 776
Size: 899 2037 1224
Fragments arranged by size:
2037 1224 899
BlpI GC'TnA_GC
Cuts at: 1272 1272
Size: 4160
Bme1580I G_kGCm'C
Cuts at: 827 1785 1878 2934 827
Size: 958 93 1056 2053
Fragments arranged by size:
2053 1056 958 93
BmrI ACTGGGnnnn_n'
Cuts at: 288 1372 1453 1788 3932 288
Size: 1084 81 335 2144 516
Fragments arranged by size:
2144 1084 516 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 955 163
Size: 792 3368
Bpu10I CC'TnA_GC
Cuts at: 895 895
Size: 4160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1336 2215 2707 3005 163
Size: 1173 879 492 298 1318
Fragments arranged by size:
1318 1173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1087 3864 1087
Size: 2777 1383
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 940 1591 1839 2541 4037 940
Size: 651 248 702 1496 1063
Fragments arranged by size:
1496 1063 702 651 248
BsaWI w'CCGG_w
Cuts at: 963 999 1349 1870 2548 2822 2969 3380 963
Size: 36 350 521 678 274 147 411 1743
Fragments arranged by size:
1743 678 521 411 350 274 147 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 953 983 1000 1030 953
Size: 30 17 30 4083
Fragments arranged by size:
4083 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 1003 160
Size: 843 3317
BseYI C'CCAG_C
Cuts at: 826 1062 1585 2343 2920 3416 826
Size: 236 523 758 577 496 1570
Fragments arranged by size:
1570 758 577 523 496 236
BsiEI CG_ry'CG
Cuts at: 104 1702 1748 2956 104
Size: 1598 46 1208 1308
Fragments arranged by size:
1598 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 764 1174 1952 2142 2934 764
Size: 410 778 190 792 1990
Fragments arranged by size:
1990 792 778 410 190
BsmI GAATG_Cn'
Cuts at: 443 443
Size: 4160
Bsp1286I G_dGCh'C
Cuts at: 179 764 827 1174 1785 1878 1952 2142 2204
Size: 585 63 347 611 93 74 190 62
Cuts at: 2204 2934 179
Size: 730 1405
Fragments arranged by size:
1405 730 611 585 347 190 93 74
63 62
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 3336 3336
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 932 1210 1424 1726 2107 932
Size: 278 214 302 381 2985
Fragments arranged by size:
2985 381 302 278 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 111 744 2158 2339 2548 111
Size: 633 1414 181 209 1723
Fragments arranged by size:
1723 1414 633 209 181
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 2521 2521
Size: 4160
BstEII G'GTnAC_C
Cuts at: 720 975 720
Size: 255 3905
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4160
BstYI r'GATC_y
Cuts at: 1 212 776 1185 1652 1675 2010 2256 3268
Size: 211 564 409 467 23 335 246 1012
Cuts at: 3268 3354 3366 3712 1
Size: 86 12 346 449
Fragments arranged by size:
1012 564 467 449 409 346 335 246
211 86 23 12
BtgI C'CryG_G
Cuts at: 136 136
Size: 4160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 845 2110 2291 2454 845
Size: 1265 181 163 2551
Fragments arranged by size:
2551 1265 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 973 1866 2724 973
Size: 893 858 2409
Fragments arranged by size:
2409 893 858
EaeI y'GGCC_r
Cuts at: 853 1081 1745 1919 2310 2337 3682 853
Size: 228 664 174 391 27 1345 1331
Fragments arranged by size:
1345 1331 664 391 228 174 27
EagI C'GGCC_G
Cuts at: 1745 1745
Size: 4160
EarI CTCTTCn'nnn_
Cuts at: 830 862 2183 2393 830
Size: 32 1321 210 2597
Fragments arranged by size:
2597 1321 210 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 683 879 2368 2678 2824 3970 683
Size: 196 1489 310 146 1146 873
Fragments arranged by size:
1489 1146 873 310 196 146
EcoICRI GAG'CTC
Cuts at: 762 1172 762
Size: 410 3750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 408 955 1985 2417 3164 3432 163
Size: 245 547 1030 432 747 268 891
Fragments arranged by size:
1030 891 747 547 432 268 245
EcoO109I rG'GnC_Cy
Cuts at: 1299 1299
Size: 4160
EcoRI G'AATT_C
Cuts at: 363 1164 363
Size: 801 3359
EcoRV GAT'ATC
Cuts at: 1128 1128
Size: 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 4160
FspI TGC'GCA
Cuts at: 127 1941 127
Size: 1814 2346
FspAI rTGC'GCAy
Cuts at: 127 127
Size: 4160
HaeII r_GCGC'y
Cuts at: 1594 1842 2864 3843 1594
Size: 248 1022 979 1911
Fragments arranged by size:
1911 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2002 2032 2034 2064 2002
Size: 30 2 30 4098
Fragments arranged by size:
4098 30 30 2
HincII GTy'rAC
Cuts at: 1199 1199
Size: 4160
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 980 2831 3015 185
Size: 795 1851 184 1330
Fragments arranged by size:
1851 1330 795 184
MscI TGG'CCA
Cuts at: 1921 1921
Size: 4160
MslI CAynn'nnrTG
Cuts at: 112 2149 2276 112
Size: 2037 127 1996
Fragments arranged by size:
2037 1996 127
MspA1I CmG'CkG
Cuts at: 1271 1585 1945 2958 3203 1271
Size: 314 360 1013 245 2228
Fragments arranged by size:
2228 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2341 2341
Size: 4160
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NgoMIV G'CCGG_C
Cuts at: 2339 2339
Size: 4160
NsiI A_TGCA'T
Cuts at: 227 227
Size: 4160
NspI r_CATG'y
Cuts at: 132 229 1213 1713 2620 132
Size: 97 984 500 907 1672
Fragments arranged by size:
1672 984 907 500 97
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PflMI CCAn_nnn'nTGG
Cuts at: 335 335
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PspXI vC'TCGA_Gb
Cuts at: 911 911
Size: 4160
PstI C_TGCA'G
Cuts at: 776 1207 1892 776
Size: 431 685 3044
Fragments arranged by size:
3044 685 431
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 1585 1945 1585
Size: 360 3800
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 764 1174 764
Size: 410 3750
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 772 1203 1888 2881 3072 33
Size: 739 431 685 993 191 1121
Fragments arranged by size:
1121 993 739 685 431 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 173 1099 1182 173
Size: 926 83 3151
Fragments arranged by size:
3151 926 83
SmlI C'TyrA_G
Cuts at: 178 911 1315 2230 2722 2984 178
Size: 733 404 915 492 262 1354
Fragments arranged by size:
1354 915 733 492 404 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 132 229 1213 1713 132
Size: 97 984 500 2579
Fragments arranged by size:
2579 984 500 97
SspI AAT'ATT
Cuts at: 876 876
Size: 4160
StyI C'CwwG_G
Cuts at: 1294 1648 1294
Size: 354 3806
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 230 1147 1743 1876 2335 2544 230
Size: 917 596 133 459 209 1846
Fragments arranged by size:
1846 917 596 459 209 133
TatI w'GTAC_w
Cuts at: 3675 3675
Size: 4160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4128
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1655 1655
Size: 4160
XhoI C'TCGA_G
Cuts at: 911 911
Size: 4160
XmaI C'CCGG_G
Cuts at: 171 1097 1180 171
Size: 926 83 3151
Fragments arranged by size:
3151 926 83
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BmgBI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XmnI ZraI