Restriction map for
pF1KB7694
(Circular) (Six-base) MAPSORT of: pF1KB7694.seq Check: 5293 from: 1 to: 4352
pF1KB7694 4352 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1390 1390
Size: 4352
Acc65I G'GTAC_C
Cuts at: 1368 1368
Size: 4352
AclI AA'CG_TT
Cuts at: 4192 4192
Size: 4352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1142 2177 2609 3356 3624 1142
Size: 1035 432 747 268 1870
Fragments arranged by size:
1870 1035 747 432 268
AflIII A'CryG_T
Cuts at: 1704 2808 3785 1704
Size: 1104 977 2271
Fragments arranged by size:
2271 1104 977
AgeI A'CCGG_T
Cuts at: 2740 2740
Size: 4352
AleI CACnn'nnGTG
Cuts at: 1324 1324
Size: 4352
AlwNI CAG_nnn'CTG
Cuts at: 1186 1231 1657 3224 1186
Size: 45 426 1567 2314
Fragments arranged by size:
2314 1567 426 45
ApaI G_GGCC'C
Cuts at: 604 1177 1213 604
Size: 573 36 3743
Fragments arranged by size:
3743 573 36
ApaLI G'TGCA_C
Cuts at: 879 3122 879
Size: 2243 2109
ApoI r'AATT_y
Cuts at: 1356 3705 3961 1356
Size: 2349 256 1747
Fragments arranged by size:
2349 1747 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4352
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
AvaI C'yCGr_G
Cuts at: 255 1372 255
Size: 1117 3235
BamHI G'GATC_C
Cuts at: 165 365 933 1377 165
Size: 200 568 444 3140
Fragments arranged by size:
3140 568 444 200
BanI G'GyrC_C
Cuts at: 1368 1782 2030 2065 1368
Size: 414 248 35 3655
Fragments arranged by size:
3655 414 248 35
BanII G_rGCy'C
Cuts at: 149 379 416 535 604 643 1063 1177 1213
Size: 230 37 119 69 39 420 114 36
Cuts at: 1213 1273 1285 1366 2396 149
Size: 60 12 81 1030 2105
Fragments arranged by size:
2105 1030 420 230 119 114 81 69
60 39 37 36 12
BbeI G_GCGC'C
Cuts at: 1786 2034 1786
Size: 248 4104
BbsI GAAGACnn'nnnn_
Cuts at: 523 523
Size: 4352
BbvCI CC'TCA_GC
Cuts at: 305 305
Size: 4352
BciVI GTATCCnnnnn_n'
Cuts at: 2250 3011 4352 2250
Size: 761 1341 2250
Fragments arranged by size:
2250 1341 761
BclI T'GATC_A
Cuts at: 1872 1872
Size: 4352
BglI GCCn_nnn'nGGC
Cuts at: 394 394
Size: 4352
BglII A'GATC_T
Cuts at: 474 1867 3904 474
Size: 1393 2037 922
Fragments arranged by size:
2037 1393 922
BlpI GC'TnA_GC
Cuts at: 529 1339 1464 529
Size: 810 125 3417
Fragments arranged by size:
3417 810 125
Bme1580I G_kGCm'C
Cuts at: 604 883 1177 1213 1977 2070 3126 604
Size: 279 294 36 764 93 1056 1830
Fragments arranged by size:
1830 1056 764 294 279 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 150 319 1180 1276 1564 1645 1980 4124 150
Size: 169 861 96 288 81 335 2144 378
Fragments arranged by size:
2144 861 378 335 288 169 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1047 526
Size: 521 3831
Bpu10I CC'TnA_GC
Cuts at: 305 305
Size: 4352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 2407 2899 3197 1528
Size: 879 492 298 2683
Fragments arranged by size:
2683 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4056 4056
Size: 4352
BsaAI yAC'GTr
Cuts at: 1705 1705
Size: 4352
BsaBI GATnn'nnATC
Cuts at: 6 1866 3903 6
Size: 1860 2037 455
Fragments arranged by size:
2037 1860 455
BsaHI Gr'CG_yC
Cuts at: 1783 2031 2733 4229 1783
Size: 248 702 1496 1906
Fragments arranged by size:
1906 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1541 2062 2740 3014 3161 3572 1541
Size: 521 678 274 147 411 2321
Fragments arranged by size:
2321 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 726 756 304
Size: 30 392 30 3900
Fragments arranged by size:
3900 392 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 446 446
Size: 4352
BseYI C'CCAG_C
Cuts at: 392 1120 1777 2535 3112 3608 392
Size: 728 657 758 577 496 1136
Fragments arranged by size:
1136 758 728 657 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 4352
BsiEI CG_ry'CG
Cuts at: 104 1894 1940 3148 104
Size: 1790 46 1208 1308
Fragments arranged by size:
1790 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 262 883 1366 2144 2334 3126 262
Size: 621 483 778 190 792 1488
Fragments arranged by size:
1488 792 778 621 483 190
Bsp1286I G_dGCh'C
Cuts at: 149 262 379 416 535 604 643 883 1063
Size: 113 117 37 119 69 39 240 180
Cuts at: 1063 1177 1213 1273 1285 1366 1977 2070 2144
Size: 114 36 60 12 81 611 93 74
Cuts at: 2144 2334 2396 3126 149
Size: 190 62 730 1375
Fragments arranged by size:
1375 730 611 240 190 180 119 117
114 113 93 81 74 69 62 60
39 37 36 12
BspEI T'CCGG_A
Cuts at: 3572 3572
Size: 4352
BspHI T'CATG_A
Cuts at: 3528 3528
Size: 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 564 859 1402 1616 1918 2299 564
Size: 295 543 214 302 381 2617
Fragments arranged by size:
2617 543 381 302 295 214
BsrBI CCG'CTC
Cuts at: 50 525 1338 2644 2698 4182 4347 50
Size: 475 813 1306 54 1484 165 55
Fragments arranged by size:
1484 1306 813 475 165 55 54
BsrDI GCAATG_nn'
Cuts at: 343 951 2264 343
Size: 608 1313 2431
Fragments arranged by size:
2431 1313 608
BsrFI r'CCGG_y
Cuts at: 388 983 2350 2531 2740 388
Size: 595 1367 181 209 2000
Fragments arranged by size:
2000 1367 595 209 181
BssSI C'ACGA_G
Cuts at: 2623 2981 2623
Size: 358 3994
BstAPI GCAn_nnn'nTGC
Cuts at: 788 887 950 1729 788
Size: 99 63 779 3411
Fragments arranged by size:
3411 779 99 63
BstBI TT'CG_AA
Cuts at: 2713 2713
Size: 4352
BstXI CCAn_nnnn'nTGG
Cuts at: 504 504
Size: 4352
BstYI r'GATC_y
Cuts at: 1 165 365 474 933 1377 1844 1867 2202
Size: 164 200 109 459 444 467 23 335
Cuts at: 2202 2448 3460 3546 3558 3904 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 459 449 444 346 335 246
200 164 109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 221 221
Size: 4352
BtgI C'CryG_G
Cuts at: 107 467 1277 107
Size: 360 810 3182
Fragments arranged by size:
3182 810 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2302 2483 2646 2302
Size: 181 163 4008
Fragments arranged by size:
4008 181 163
BtsI GCAGTG_nn'
Cuts at: 248 559 1595 248
Size: 311 1036 3005
Fragments arranged by size:
3005 1036 311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 660 695 660
Size: 35 4317
DraI TTT'AAA
Cuts at: 1351 1351
Size: 4352
DraIII CAC_nnn'GTG
Cuts at: 978 978
Size: 4352
DrdI GACnn_nn'nnGTC
Cuts at: 2058 2916 2058
Size: 858 3494
EaeI y'GGCC_r
Cuts at: 985 1937 2111 2502 2529 3874 985
Size: 952 174 391 27 1345 1463
Fragments arranged by size:
1463 1345 952 391 174 27
EagI C'GGCC_G
Cuts at: 1937 1937
Size: 4352
EarI CTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 768 930 2560 2870 3016 4162 768
Size: 162 1630 310 146 1146 958
Fragments arranged by size:
1630 1146 958 310 162 146
EcoICRI GAG'CTC
Cuts at: 1364 1364
Size: 4352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1047 1142 2177 2609 3356 3624 526
Size: 521 95 1035 432 747 268 1254
Fragments arranged by size:
1254 1035 747 521 432 268 95
EcoNI CCTnn'n_nnAGG
Cuts at: 294 294
Size: 4352
EcoO109I rG'GnC_Cy
Cuts at: 128 137 553 600 601 824 1174 1210 1228
Size: 9 416 47 1 223 350 36 18
Cuts at: 1228 1297 1491 128
Size: 69 194 2989
Fragments arranged by size:
2989 416 350 223 194 69 47 36
18 9 1
EcoRI G'AATT_C
Cuts at: 1356 1356
Size: 4352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4352
FspI TGC'GCA
Cuts at: 2133 2133
Size: 4352
HaeII r_GCGC'y
Cuts at: 849 1786 2034 3056 4035 849
Size: 937 248 1022 979 1166
Fragments arranged by size:
1166 1022 979 937 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2194 2224 2226 2256 2194
Size: 30 2 30 4290
Fragments arranged by size:
4290 30 30 2
HincII GTy'rAC
Cuts at: 1391 1391
Size: 4352
HindIII A'AGCT_T
Cuts at: 673 673
Size: 4352
KasI G'GCGC_C
Cuts at: 1782 2030 1782
Size: 248 4104
KpnI G_GTAC'C
Cuts at: 1372 1372
Size: 4352
MluI A'CGCG_T
Cuts at: 3785 3785
Size: 4352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3023 3207 3023
Size: 184 4168
MscI TGG'CCA
Cuts at: 2113 2113
Size: 4352
MslI CAynn'nnrTG
Cuts at: 1324 2341 2468 1324
Size: 1017 127 3208
Fragments arranged by size:
3208 1017 127
MspA1I CmG'CkG
Cuts at: 271 277 1099 1130 1463 1777 2137 3150 3395
Size: 6 822 31 333 314 360 1013 245
Cuts at: 3395 271
Size: 1228
Fragments arranged by size:
1228 1013 822 360 333 314 245 31
6
NaeI GCC'GGC
Cuts at: 390 2533 390
Size: 2143 2209
NarI GG'CG_CC
Cuts at: 1783 2031 1783
Size: 248 4104
NcoI C'CATG_G
Cuts at: 107 107
Size: 4352
NdeI CA'TA_TG
Cuts at: 384 384
Size: 4352
NgoMIV G'CCGG_C
Cuts at: 388 2531 388
Size: 2143 2209
NspI r_CATG'y
Cuts at: 1405 1905 2812 1405
Size: 500 907 2945
Fragments arranged by size:
2945 907 500
PasI CC'CwG_GG
Cuts at: 132 370 549 132
Size: 238 179 3935
Fragments arranged by size:
3935 238 179
PciI A'CATG_T
Cuts at: 2808 2808
Size: 4352
PflMI CCAn_nnn'nTGG
Cuts at: 162 468 1102 1206 162
Size: 306 634 104 3308
Fragments arranged by size:
3308 634 306 104
PfoI T'CCnGG_A
Cuts at: 503 503
Size: 4352
PmeI GTTT'AAAC
Cuts at: 1351 1351
Size: 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 3491
Size: 32 4320
PpuMI rG'GwC_Cy
Cuts at: 128 137 553 128
Size: 9 416 3927
Fragments arranged by size:
3927 416 9
PspOMI G'GGCC_C
Cuts at: 600 1173 1209 600
Size: 573 36 3743
Fragments arranged by size:
3743 573 36
PstI C_TGCA'G
Cuts at: 276 1399 2084 276
Size: 1123 685 2544
Fragments arranged by size:
2544 1123 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4352
PvuII CAG'CTG
Cuts at: 277 1099 1777 2137 277
Size: 822 678 360 2492
Fragments arranged by size:
2492 822 678 360
RsrII CG'GwC_CG
Cuts at: 2547 2547
Size: 4352
SacI G_AGCT'C
Cuts at: 1366 1366
Size: 4352
SalI G'TCGA_C
Cuts at: 1389 1389
Size: 4352
SanDI GG'GwC_CC
Cuts at: 128 128
Size: 4352
SapI GCTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
SbfI CC_TGCA'GG
Cuts at: 1399 1399
Size: 4352
ScaI AGT'ACT
Cuts at: 3869 3869
Size: 4352
SexAI A'CCwGG_T
Cuts at: 974 974
Size: 4352
SfcI C'TryA_G
Cuts at: 33 272 294 312 1395 2080 3073 3264 33
Size: 239 22 18 1083 685 993 191 1121
Fragments arranged by size:
1121 1083 993 685 239 191 22 18
SfoI GGC'GCC
Cuts at: 1784 2032 1784
Size: 248 4104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
SmaI CCC'GGG
Cuts at: 1374 1374
Size: 4352
SmlI C'TyrA_G
Cuts at: 1507 2422 2914 3176 1507
Size: 915 492 262 2683
Fragments arranged by size:
2683 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4352
SphI G_CATG'C
Cuts at: 1405 1905 1405
Size: 500 3852
StuI AGG'CCT
Cuts at: 310 1163 1205 310
Size: 853 42 3457
Fragments arranged by size:
3457 853 42
StyI C'CwwG_G
Cuts at: 107 1200 1486 1840 107
Size: 1093 286 354 2619
Fragments arranged by size:
2619 1093 354 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1290 1935 2068 2527 2736 1290
Size: 645 133 459 209 2906
Fragments arranged by size:
2906 645 459 209 133
TatI w'GTAC_w
Cuts at: 3867 3867
Size: 4352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 563 595 925 957 3869 3901 563
Size: 32 330 32 2912 32 1014
Fragments arranged by size:
2912 1014 330 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2149 2149
Size: 4352
XbaI T'CTAG_A
Cuts at: 1383 1383
Size: 4352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 389 1323 1847 389
Size: 934 524 2894
Fragments arranged by size:
2894 934 524
XmaI C'CCGG_G
Cuts at: 1372 1372
Size: 4352
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI