(Circular) (Six-base) MAPSORT of: pF1KB7713.seq Check: 5854 from: 1 to: 4382 pF1KB7713 4382 bp With 182 enzymes: SgfI * June 26, 2008 10:49 .. AccI GT'mk_AC Cuts at: 1420 1420 Size: 4382 Acc65I G'GTAC_C Cuts at: 1398 1398 Size: 4382 AclI AA'CG_TT Cuts at: 4222 4222 Size: 4382 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 2639 3386 3654 2207 Size: 432 747 268 2935 Fragments arranged by size: 2935 747 432 268 AfeI AGC'GCT Cuts at: 934 934 Size: 4382 AflIII A'CryG_T Cuts at: 1734 2838 3815 1734 Size: 1104 977 2301 Fragments arranged by size: 2301 1104 977 AgeI A'CCGG_T Cuts at: 2770 2770 Size: 4382 AhdI GACnn_n'nnGTC Cuts at: 879 879 Size: 4382 AleI CACnn'nnGTG Cuts at: 380 380 Size: 4382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1267 1267 Size: 4382 AlwNI CAG_nnn'CTG Cuts at: 658 760 1062 1123 1687 3254 658 Size: 102 302 61 564 1567 1786 Fragments arranged by size: 1786 1567 564 302 102 61 ApaI G_GGCC'C Cuts at: 631 647 631 Size: 16 4366 ApaLI G'TGCA_C Cuts at: 1126 3152 1126 Size: 2026 2356 ApoI r'AATT_y Cuts at: 1386 3735 3991 1386 Size: 2349 256 1777 Fragments arranged by size: 2349 1777 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4382 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4382 AvaI C'yCGr_G Cuts at: 624 1360 1402 624 Size: 736 42 3604 Fragments arranged by size: 3604 736 42 BamHI G'GATC_C Cuts at: 1407 1407 Size: 4382 BanI G'GyrC_C Cuts at: 767 1329 1398 1812 2060 2095 767 Size: 562 69 414 248 35 3054 Fragments arranged by size: 3054 562 414 248 69 35 BanII G_rGCy'C Cuts at: 631 647 1396 2426 631 Size: 16 749 1030 2587 Fragments arranged by size: 2587 1030 749 16 BbeI G_GCGC'C Cuts at: 1816 2064 1816 Size: 248 4134 BbsI GAAGACnn'nnnn_ Cuts at: 679 679 Size: 4382 BbvCI CC'TCA_GC Cuts at: 968 968 Size: 4382 BciVI GTATCCnnnnn_n' Cuts at: 2280 3041 4382 2280 Size: 761 1341 2280 Fragments arranged by size: 2280 1341 761 BclI T'GATC_A Cuts at: 1902 1902 Size: 4382 BglI GCCn_nnn'nGGC Cuts at: 251 502 1204 1208 251 Size: 251 702 4 3425 Fragments arranged by size: 3425 702 251 4 BglII A'GATC_T Cuts at: 1897 3934 1897 Size: 2037 2345 BlpI GC'TnA_GC Cuts at: 319 1226 1494 319 Size: 907 268 3207 Fragments arranged by size: 3207 907 268 Bme1580I G_kGCm'C Cuts at: 631 647 770 999 1130 1334 2007 2100 3156 Size: 16 123 229 131 204 673 93 1056 Cuts at: 3156 631 Size: 1857 Fragments arranged by size: 1857 1056 673 229 204 131 123 93 16 BmgBI CAC'GTC Cuts at: 360 360 Size: 4382 BmrI ACTGGGnnnn_n' Cuts at: 679 1030 1594 1675 2010 4154 679 Size: 351 564 81 335 2144 907 Fragments arranged by size: 2144 907 564 351 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2749 2749 Size: 4382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 680 1090 1207 1339 680 Size: 410 117 132 3723 Fragments arranged by size: 3723 410 132 117 Bpu10I CC'TnA_GC Cuts at: 968 968 Size: 4382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 526 1558 2437 2929 3227 526 Size: 1032 879 492 298 1681 Fragments arranged by size: 1681 1032 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 190 4086 190 Size: 3896 486 BsaAI yAC'GTr Cuts at: 1735 1735 Size: 4382 BsaBI GATnn'nnATC Cuts at: 6 1896 3933 6 Size: 1890 2037 455 Fragments arranged by size: 2037 1890 455 BsaHI Gr'CG_yC Cuts at: 1813 2061 2763 4259 1813 Size: 248 702 1496 1936 Fragments arranged by size: 1936 1496 702 248 BsaWI w'CCGG_w Cuts at: 1571 2092 2770 3044 3191 3602 1571 Size: 521 678 274 147 411 2351 Fragments arranged by size: 2351 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 189 219 349 379 1253 1283 189 Size: 30 130 30 874 30 3288 Fragments arranged by size: 3288 874 130 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 280 1093 1258 280 Size: 813 165 3404 Fragments arranged by size: 3404 813 165 BseYI C'CCAG_C Cuts at: 283 640 774 830 918 929 1807 2565 3142 Size: 357 134 56 88 11 878 758 577 Cuts at: 3142 3638 283 Size: 496 1027 Fragments arranged by size: 1027 878 758 577 496 357 134 88 56 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1156 1156 Size: 4382 BsiEI CG_ry'CG Cuts at: 104 601 1203 1924 1970 3178 104 Size: 497 602 721 46 1208 1308 Fragments arranged by size: 1308 1208 721 602 497 46 BsiHKAI G_wGCw'C Cuts at: 320 1130 1396 2174 2364 3156 320 Size: 810 266 778 190 792 1546 Fragments arranged by size: 1546 810 792 778 266 190 BsmI GAATG_Cn' Cuts at: 696 696 Size: 4382 BsmBI CGTCTCn'nnnn_ Cuts at: 480 480 Size: 4382 Bsp1286I G_dGCh'C Cuts at: 320 631 647 770 999 1130 1334 1396 2007 Size: 311 16 123 229 131 204 62 611 Cuts at: 2007 2100 2174 2364 2426 3156 320 Size: 93 74 190 62 730 1546 Fragments arranged by size: 1546 730 611 311 229 204 190 131 123 93 74 62 62 16 BspEI T'CCGG_A Cuts at: 3602 3602 Size: 4382 BspHI T'CATG_A Cuts at: 3558 3558 Size: 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1432 1646 1948 2329 1432 Size: 214 302 381 3485 Fragments arranged by size: 3485 381 302 214 BsrBI CCG'CTC Cuts at: 50 1230 1266 2674 2728 4212 4377 50 Size: 1180 36 1408 54 1484 165 55 Fragments arranged by size: 1484 1408 1180 165 55 54 36 BsrDI GCAATG_nn' Cuts at: 2294 2294 Size: 4382 BsrFI r'CCGG_y Cuts at: 156 1198 2380 2561 2770 156 Size: 1042 1182 181 209 1768 Fragments arranged by size: 1768 1182 1042 209 181 BssSI C'ACGA_G Cuts at: 1174 2653 3011 1174 Size: 1479 358 2545 Fragments arranged by size: 2545 1479 358 BstAPI GCAn_nnn'nTGC Cuts at: 497 1044 1062 1759 497 Size: 547 18 697 3120 Fragments arranged by size: 3120 697 547 18 BstBI TT'CG_AA Cuts at: 2743 2743 Size: 4382 BstEII G'GTnAC_C Cuts at: 819 819 Size: 4382 BstXI CCAn_nnnn'nTGG Cuts at: 120 1306 120 Size: 1186 3196 BstYI r'GATC_y Cuts at: 1 1407 1874 1897 2232 2478 3490 3576 3588 Size: 1406 467 23 335 246 1012 86 12 Cuts at: 3588 3934 1 Size: 346 449 Fragments arranged by size: 1406 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 779 779 Size: 4382 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 842 2332 2513 2676 842 Size: 1490 181 163 2548 Fragments arranged by size: 2548 1490 181 163 BtsI GCAGTG_nn' Cuts at: 613 937 943 1625 613 Size: 324 6 682 3370 Fragments arranged by size: 3370 682 324 6 DraI TTT'AAA Cuts at: 1381 1381 Size: 4382 DraIII CAC_nnn'GTG Cuts at: 521 521 Size: 4382 DrdI GACnn_nn'nnGTC Cuts at: 2088 2946 2088 Size: 858 3524 EaeI y'GGCC_r Cuts at: 158 598 1200 1967 2141 2532 2559 3904 158 Size: 440 602 767 174 391 27 1345 636 Fragments arranged by size: 1345 767 636 602 440 391 174 27 EagI C'GGCC_G Cuts at: 598 1200 1967 598 Size: 602 767 3013 Fragments arranged by size: 3013 767 602 EarI CTCTTCn'nnn_ Cuts at: 257 983 2405 2615 257 Size: 726 1422 210 2024 Fragments arranged by size: 2024 1422 726 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1261 2590 2900 3046 4192 1261 Size: 1329 310 146 1146 1451 Fragments arranged by size: 1451 1329 1146 310 146 EcoICRI GAG'CTC Cuts at: 1394 1394 Size: 4382 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 680 1090 1207 1339 2207 2639 3386 3654 680 Size: 410 117 132 868 432 747 268 1408 Fragments arranged by size: 1408 868 747 432 410 268 132 117 EcoO109I rG'GnC_Cy Cuts at: 628 644 664 757 1059 1179 1521 628 Size: 16 20 93 302 120 342 3489 Fragments arranged by size: 3489 342 302 120 93 20 16 EcoRI G'AATT_C Cuts at: 1386 1386 Size: 4382 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1365 2401 1365 Size: 1036 3346 FspI TGC'GCA Cuts at: 2163 2163 Size: 4382 HaeII r_GCGC'y Cuts at: 339 936 1816 2064 3086 4065 339 Size: 597 880 248 1022 979 656 Fragments arranged by size: 1022 979 880 656 597 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 189 221 349 381 865 897 2224 2254 2256 Size: 32 128 32 484 32 1327 30 2 Cuts at: 2256 2286 189 Size: 30 2285 Fragments arranged by size: 2285 1327 484 128 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1421 1421 Size: 4382 KasI G'GCGC_C Cuts at: 1812 2060 1812 Size: 248 4134 KpnI G_GTAC'C Cuts at: 1402 1402 Size: 4382 MluI A'CGCG_T Cuts at: 3815 3815 Size: 4382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 485 3053 3237 485 Size: 2568 184 1630 Fragments arranged by size: 2568 1630 184 MscI TGG'CCA Cuts at: 2143 2143 Size: 4382 MslI CAynn'nnrTG Cuts at: 380 1304 2371 2498 380 Size: 924 1067 127 2264 Fragments arranged by size: 2264 1067 924 127 MspA1I CmG'CkG Cuts at: 449 830 1006 1158 1282 1493 1807 2167 3180 Size: 381 176 152 124 211 314 360 1013 Cuts at: 3180 3425 449 Size: 245 1406 Fragments arranged by size: 1406 1013 381 360 314 245 211 176 152 124 NaeI GCC'GGC Cuts at: 158 1200 2563 158 Size: 1042 1363 1977 Fragments arranged by size: 1977 1363 1042 NarI GG'CG_CC Cuts at: 1813 2061 1813 Size: 248 4134 NgoMIV G'CCGG_C Cuts at: 156 1198 2561 156 Size: 1042 1363 1977 Fragments arranged by size: 1977 1363 1042 NspI r_CATG'y Cuts at: 540 1435 1935 2842 540 Size: 895 500 907 2080 Fragments arranged by size: 2080 907 895 500 PciI A'CATG_T Cuts at: 2838 2838 Size: 4382 PflMI CCAn_nnn'nTGG Cuts at: 123 830 123 Size: 707 3675 PfoI T'CCnGG_A Cuts at: 980 980 Size: 4382 PmeI GTTT'AAAC Cuts at: 1381 1381 Size: 4382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3521 3553 3521 Size: 32 4350 PspOMI G'GGCC_C Cuts at: 627 643 627 Size: 16 4366 PspXI vC'TCGA_Gb Cuts at: 1360 1360 Size: 4382 PstI C_TGCA'G Cuts at: 893 950 1058 1429 2114 893 Size: 57 108 371 685 3161 Fragments arranged by size: 3161 685 371 108 57 PvuI CG_AT'CG Cuts at: 104 104 Size: 4382 PvuII CAG'CTG Cuts at: 449 830 1006 1158 1807 2167 449 Size: 381 176 152 649 360 2664 Fragments arranged by size: 2664 649 381 360 176 152 RsrII CG'GwC_CG Cuts at: 2577 2577 Size: 4382 SacI G_AGCT'C Cuts at: 1396 1396 Size: 4382 SalI G'TCGA_C Cuts at: 1419 1419 Size: 4382 SapI GCTCTTCn'nnn_ Cuts at: 2405 2615 2405 Size: 210 4172 SbfI CC_TGCA'GG Cuts at: 1429 1429 Size: 4382 ScaI AGT'ACT Cuts at: 887 3899 887 Size: 3012 1370 SfcI C'TryA_G Cuts at: 33 889 946 1054 1425 2110 3103 3294 33 Size: 856 57 108 371 685 993 191 1121 Fragments arranged by size: 1121 993 856 685 371 191 108 57 SfiI GGCCn_nnn'nGGCC Cuts at: 502 1208 502 Size: 706 3676 SfoI GGC'GCC Cuts at: 1814 2062 1814 Size: 248 4134 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4382 SmaI CCC'GGG Cuts at: 626 1404 626 Size: 778 3604 SmlI C'TyrA_G Cuts at: 541 1360 1537 2452 2944 3206 541 Size: 819 177 915 492 262 1717 Fragments arranged by size: 1717 915 819 492 262 177 SpeI A'CTAG_T Cuts at: 67 1041 67 Size: 974 3408 SphI G_CATG'C Cuts at: 540 1435 1935 540 Size: 895 500 2987 Fragments arranged by size: 2987 895 500 StuI AGG'CCT Cuts at: 400 496 505 1099 400 Size: 96 9 594 3683 Fragments arranged by size: 3683 594 96 9 StyI C'CwwG_G Cuts at: 1100 1516 1870 1100 Size: 416 354 3612 Fragments arranged by size: 3612 416 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 214 821 910 1965 2098 2557 2766 214 Size: 607 89 1055 133 459 209 1830 Fragments arranged by size: 1830 1055 607 459 209 133 89 TatI w'GTAC_w Cuts at: 443 587 885 3897 443 Size: 144 298 3012 928 Fragments arranged by size: 3012 928 298 144 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 605 637 3899 3931 605 Size: 32 3262 32 1056 Fragments arranged by size: 3262 1056 32 32 Tth111I GACn'n_nGTC Cuts at: 565 976 2179 565 Size: 411 1203 2768 Fragments arranged by size: 2768 1203 411 XbaI T'CTAG_A Cuts at: 1413 1413 Size: 4382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 120 1877 120 Size: 1757 2625 XhoI C'TCGA_G Cuts at: 1360 1360 Size: 4382 XmaI C'CCGG_G Cuts at: 624 1402 624 Size: 778 3604 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AflII AloI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI