Restriction map for
pF1KB7713
(Circular) (Six-base) MAPSORT of: pF1KB7713.seq Check: 5854 from: 1 to: 4382
pF1KB7713 4382 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 2639 3386 3654 2207
Size: 432 747 268 2935
Fragments arranged by size:
2935 747 432 268
AfeI AGC'GCT
Cuts at: 934 934
Size: 4382
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AhdI GACnn_n'nnGTC
Cuts at: 879 879
Size: 4382
AleI CACnn'nnGTG
Cuts at: 380 380
Size: 4382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1267 1267
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 658 760 1062 1123 1687 3254 658
Size: 102 302 61 564 1567 1786
Fragments arranged by size:
1786 1567 564 302 102 61
ApaI G_GGCC'C
Cuts at: 631 647 631
Size: 16 4366
ApaLI G'TGCA_C
Cuts at: 1126 3152 1126
Size: 2026 2356
ApoI r'AATT_y
Cuts at: 1386 3735 3991 1386
Size: 2349 256 1777
Fragments arranged by size:
2349 1777 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 624 1360 1402 624
Size: 736 42 3604
Fragments arranged by size:
3604 736 42
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 767 1329 1398 1812 2060 2095 767
Size: 562 69 414 248 35 3054
Fragments arranged by size:
3054 562 414 248 69 35
BanII G_rGCy'C
Cuts at: 631 647 1396 2426 631
Size: 16 749 1030 2587
Fragments arranged by size:
2587 1030 749 16
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 679 679
Size: 4382
BbvCI CC'TCA_GC
Cuts at: 968 968
Size: 4382
BciVI GTATCCnnnnn_n'
Cuts at: 2280 3041 4382 2280
Size: 761 1341 2280
Fragments arranged by size:
2280 1341 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BglI GCCn_nnn'nGGC
Cuts at: 251 502 1204 1208 251
Size: 251 702 4 3425
Fragments arranged by size:
3425 702 251 4
BglII A'GATC_T
Cuts at: 1897 3934 1897
Size: 2037 2345
BlpI GC'TnA_GC
Cuts at: 319 1226 1494 319
Size: 907 268 3207
Fragments arranged by size:
3207 907 268
Bme1580I G_kGCm'C
Cuts at: 631 647 770 999 1130 1334 2007 2100 3156
Size: 16 123 229 131 204 673 93 1056
Cuts at: 3156 631
Size: 1857
Fragments arranged by size:
1857 1056 673 229 204 131 123 93
16
BmgBI CAC'GTC
Cuts at: 360 360
Size: 4382
BmrI ACTGGGnnnn_n'
Cuts at: 679 1030 1594 1675 2010 4154 679
Size: 351 564 81 335 2144 907
Fragments arranged by size:
2144 907 564 351 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2749 2749
Size: 4382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 1090 1207 1339 680
Size: 410 117 132 3723
Fragments arranged by size:
3723 410 132 117
Bpu10I CC'TnA_GC
Cuts at: 968 968
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 1558 2437 2929 3227 526
Size: 1032 879 492 298 1681
Fragments arranged by size:
1681 1032 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 190 4086 190
Size: 3896 486
BsaAI yAC'GTr
Cuts at: 1735 1735
Size: 4382
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1813 2061 2763 4259 1813
Size: 248 702 1496 1936
Fragments arranged by size:
1936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 189 219 349 379 1253 1283 189
Size: 30 130 30 874 30 3288
Fragments arranged by size:
3288 874 130 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 1093 1258 280
Size: 813 165 3404
Fragments arranged by size:
3404 813 165
BseYI C'CCAG_C
Cuts at: 283 640 774 830 918 929 1807 2565 3142
Size: 357 134 56 88 11 878 758 577
Cuts at: 3142 3638 283
Size: 496 1027
Fragments arranged by size:
1027 878 758 577 496 357 134 88
56 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1156
Size: 4382
BsiEI CG_ry'CG
Cuts at: 104 601 1203 1924 1970 3178 104
Size: 497 602 721 46 1208 1308
Fragments arranged by size:
1308 1208 721 602 497 46
BsiHKAI G_wGCw'C
Cuts at: 320 1130 1396 2174 2364 3156 320
Size: 810 266 778 190 792 1546
Fragments arranged by size:
1546 810 792 778 266 190
BsmI GAATG_Cn'
Cuts at: 696 696
Size: 4382
BsmBI CGTCTCn'nnnn_
Cuts at: 480 480
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 320 631 647 770 999 1130 1334 1396 2007
Size: 311 16 123 229 131 204 62 611
Cuts at: 2007 2100 2174 2364 2426 3156 320
Size: 93 74 190 62 730 1546
Fragments arranged by size:
1546 730 611 311 229 204 190 131
123 93 74 62 62 16
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1230 1266 2674 2728 4212 4377 50
Size: 1180 36 1408 54 1484 165 55
Fragments arranged by size:
1484 1408 1180 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 2294 2294
Size: 4382
BsrFI r'CCGG_y
Cuts at: 156 1198 2380 2561 2770 156
Size: 1042 1182 181 209 1768
Fragments arranged by size:
1768 1182 1042 209 181
BssSI C'ACGA_G
Cuts at: 1174 2653 3011 1174
Size: 1479 358 2545
Fragments arranged by size:
2545 1479 358
BstAPI GCAn_nnn'nTGC
Cuts at: 497 1044 1062 1759 497
Size: 547 18 697 3120
Fragments arranged by size:
3120 697 547 18
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstEII G'GTnAC_C
Cuts at: 819 819
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 120 1306 120
Size: 1186 3196
BstYI r'GATC_y
Cuts at: 1 1407 1874 1897 2232 2478 3490 3576 3588
Size: 1406 467 23 335 246 1012 86 12
Cuts at: 3588 3934 1
Size: 346 449
Fragments arranged by size:
1406 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 779 779
Size: 4382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 842 2332 2513 2676 842
Size: 1490 181 163 2548
Fragments arranged by size:
2548 1490 181 163
BtsI GCAGTG_nn'
Cuts at: 613 937 943 1625 613
Size: 324 6 682 3370
Fragments arranged by size:
3370 682 324 6
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DraIII CAC_nnn'GTG
Cuts at: 521 521
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 158 598 1200 1967 2141 2532 2559 3904 158
Size: 440 602 767 174 391 27 1345 636
Fragments arranged by size:
1345 767 636 602 440 391 174 27
EagI C'GGCC_G
Cuts at: 598 1200 1967 598
Size: 602 767 3013
Fragments arranged by size:
3013 767 602
EarI CTCTTCn'nnn_
Cuts at: 257 983 2405 2615 257
Size: 726 1422 210 2024
Fragments arranged by size:
2024 1422 726 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1261 2590 2900 3046 4192 1261
Size: 1329 310 146 1146 1451
Fragments arranged by size:
1451 1329 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 1090 1207 1339 2207 2639 3386 3654 680
Size: 410 117 132 868 432 747 268 1408
Fragments arranged by size:
1408 868 747 432 410 268 132 117
EcoO109I rG'GnC_Cy
Cuts at: 628 644 664 757 1059 1179 1521 628
Size: 16 20 93 302 120 342 3489
Fragments arranged by size:
3489 342 302 120 93 20 16
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1365 2401 1365
Size: 1036 3346
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 339 936 1816 2064 3086 4065 339
Size: 597 880 248 1022 979 656
Fragments arranged by size:
1022 979 880 656 597 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 189 221 349 381 865 897 2224 2254 2256
Size: 32 128 32 484 32 1327 30 2
Cuts at: 2256 2286 189
Size: 30 2285
Fragments arranged by size:
2285 1327 484 128 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1421 1421
Size: 4382
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 485 3053 3237 485
Size: 2568 184 1630
Fragments arranged by size:
2568 1630 184
MscI TGG'CCA
Cuts at: 2143 2143
Size: 4382
MslI CAynn'nnrTG
Cuts at: 380 1304 2371 2498 380
Size: 924 1067 127 2264
Fragments arranged by size:
2264 1067 924 127
MspA1I CmG'CkG
Cuts at: 449 830 1006 1158 1282 1493 1807 2167 3180
Size: 381 176 152 124 211 314 360 1013
Cuts at: 3180 3425 449
Size: 245 1406
Fragments arranged by size:
1406 1013 381 360 314 245 211 176
152 124
NaeI GCC'GGC
Cuts at: 158 1200 2563 158
Size: 1042 1363 1977
Fragments arranged by size:
1977 1363 1042
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NgoMIV G'CCGG_C
Cuts at: 156 1198 2561 156
Size: 1042 1363 1977
Fragments arranged by size:
1977 1363 1042
NspI r_CATG'y
Cuts at: 540 1435 1935 2842 540
Size: 895 500 907 2080
Fragments arranged by size:
2080 907 895 500
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 123 830 123
Size: 707 3675
PfoI T'CCnGG_A
Cuts at: 980 980
Size: 4382
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PspOMI G'GGCC_C
Cuts at: 627 643 627
Size: 16 4366
PspXI vC'TCGA_Gb
Cuts at: 1360 1360
Size: 4382
PstI C_TGCA'G
Cuts at: 893 950 1058 1429 2114 893
Size: 57 108 371 685 3161
Fragments arranged by size:
3161 685 371 108 57
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 449 830 1006 1158 1807 2167 449
Size: 381 176 152 649 360 2664
Fragments arranged by size:
2664 649 381 360 176 152
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 887 3899 887
Size: 3012 1370
SfcI C'TryA_G
Cuts at: 33 889 946 1054 1425 2110 3103 3294 33
Size: 856 57 108 371 685 993 191 1121
Fragments arranged by size:
1121 993 856 685 371 191 108 57
SfiI GGCCn_nnn'nGGCC
Cuts at: 502 1208 502
Size: 706 3676
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 626 1404 626
Size: 778 3604
SmlI C'TyrA_G
Cuts at: 541 1360 1537 2452 2944 3206 541
Size: 819 177 915 492 262 1717
Fragments arranged by size:
1717 915 819 492 262 177
SpeI A'CTAG_T
Cuts at: 67 1041 67
Size: 974 3408
SphI G_CATG'C
Cuts at: 540 1435 1935 540
Size: 895 500 2987
Fragments arranged by size:
2987 895 500
StuI AGG'CCT
Cuts at: 400 496 505 1099 400
Size: 96 9 594 3683
Fragments arranged by size:
3683 594 96 9
StyI C'CwwG_G
Cuts at: 1100 1516 1870 1100
Size: 416 354 3612
Fragments arranged by size:
3612 416 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 821 910 1965 2098 2557 2766 214
Size: 607 89 1055 133 459 209 1830
Fragments arranged by size:
1830 1055 607 459 209 133 89
TatI w'GTAC_w
Cuts at: 443 587 885 3897 443
Size: 144 298 3012 928
Fragments arranged by size:
3012 928 298 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 605 637 3899 3931 605
Size: 32 3262 32 1056
Fragments arranged by size:
3262 1056 32 32
Tth111I GACn'n_nGTC
Cuts at: 565 976 2179 565
Size: 411 1203 2768
Fragments arranged by size:
2768 1203 411
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 1877 120
Size: 1757 2625
XhoI C'TCGA_G
Cuts at: 1360 1360
Size: 4382
XmaI C'CCGG_G
Cuts at: 624 1402 624
Size: 778 3604
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsrGI BssHII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsiI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI