Restriction map for
pF1KB7714
(Circular) (Six-base) MAPSORT of: pF1KB7714.seq Check: 7038 from: 1 to: 4532
pF1KB7714 4532 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 165 1150 165
Size: 985 3547
AccI GT'mk_AC
Cuts at: 1570 1570
Size: 4532
Acc65I G'GTAC_C
Cuts at: 1548 1548
Size: 4532
AclI AA'CG_TT
Cuts at: 4372 4372
Size: 4532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 1072 1234 1363 1417 2357 2789 3536 3804
Size: 73 162 129 54 940 432 747 268
Cuts at: 3804 999
Size: 1727
Fragments arranged by size:
1727 940 747 432 268 162 129 73
54
AflIII A'CryG_T
Cuts at: 394 959 971 1884 2988 3965 394
Size: 565 12 913 1104 977 961
Fragments arranged by size:
1104 977 961 913 565 12
AgeI A'CCGG_T
Cuts at: 2920 2920
Size: 4532
AhdI GACnn_n'nnGTC
Cuts at: 285 285
Size: 4532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 194 194
Size: 4532
AlwNI CAG_nnn'CTG
Cuts at: 289 636 651 767 890 1455 1837 3404 289
Size: 347 15 116 123 565 382 1567 1417
Fragments arranged by size:
1567 1417 565 382 347 123 116 15
ApaLI G'TGCA_C
Cuts at: 3302 3302
Size: 4532
ApoI r'AATT_y
Cuts at: 735 1198 1536 3885 4141 735
Size: 463 338 2349 256 1126
Fragments arranged by size:
2349 1126 463 338 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4532
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
AvaI C'yCGr_G
Cuts at: 1552 1552
Size: 4532
BamHI G'GATC_C
Cuts at: 1557 1557
Size: 4532
BanI G'GyrC_C
Cuts at: 452 469 1548 1962 2210 2245 452
Size: 17 1079 414 248 35 2739
Fragments arranged by size:
2739 1079 414 248 35 17
BanII G_rGCy'C
Cuts at: 1190 1424 1546 2576 1190
Size: 234 122 1030 3146
Fragments arranged by size:
3146 1030 234 122
BbeI G_GCGC'C
Cuts at: 473 1966 2214 473
Size: 1493 248 2791
Fragments arranged by size:
2791 1493 248
BbsI GAAGACnn'nnnn_
Cuts at: 496 1157 496
Size: 661 3871
BbvCI CC'TCA_GC
Cuts at: 448 448
Size: 4532
BciVI GTATCCnnnnn_n'
Cuts at: 2430 3191 4532 2430
Size: 761 1341 2430
Fragments arranged by size:
2430 1341 761
BclI T'GATC_A
Cuts at: 557 596 674 2052 557
Size: 39 78 1378 3037
Fragments arranged by size:
3037 1378 78 39
BglII A'GATC_T
Cuts at: 837 929 1040 2047 4084 837
Size: 92 111 1007 2037 1285
Fragments arranged by size:
2037 1285 1007 111 92
BlpI GC'TnA_GC
Cuts at: 757 1439 1644 757
Size: 682 205 3645
Fragments arranged by size:
3645 682 205
Bme1580I G_kGCm'C
Cuts at: 457 2157 2250 3306 457
Size: 1700 93 1056 1683
Fragments arranged by size:
1700 1683 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 299 646 1744 1825 2160 4304 299
Size: 347 1098 81 335 2144 527
Fragments arranged by size:
2144 1098 527 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 951 2899 951
Size: 1948 2584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 553 1204 247
Size: 306 651 3575
Fragments arranged by size:
3575 651 306
Bpu10I CC'TnA_GC
Cuts at: 448 448
Size: 4532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1708 2587 3079 3377 1708
Size: 879 492 298 2863
Fragments arranged by size:
2863 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 228 861 4236 228
Size: 633 3375 524
Fragments arranged by size:
3375 633 524
BsaAI yAC'GTr
Cuts at: 1885 1885
Size: 4532
BsaBI GATnn'nnATC
Cuts at: 6 673 2046 4083 6
Size: 667 1373 2037 455
Fragments arranged by size:
2037 1373 667 455
BsaHI Gr'CG_yC
Cuts at: 470 1963 2211 2913 4409 470
Size: 1493 248 702 1496 593
Fragments arranged by size:
1496 1493 702 593 248
BsaWI w'CCGG_w
Cuts at: 461 830 1721 2242 2920 3194 3341 3752 461
Size: 369 891 521 678 274 147 411 1241
Fragments arranged by size:
1241 891 678 521 411 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1027 1057 1027
Size: 30 4502
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 595 673 1249 1252 595
Size: 78 576 3 3875
Fragments arranged by size:
3875 576 78 3
BseYI C'CCAG_C
Cuts at: 906 1370 1448 1957 2715 3292 3788 906
Size: 464 78 509 758 577 496 1650
Fragments arranged by size:
1650 758 577 509 496 464 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 664 154
Size: 510 4022
BsiEI CG_ry'CG
Cuts at: 104 2074 2120 3328 104
Size: 1970 46 1208 1308
Fragments arranged by size:
1970 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 156 847 1424 1546 2324 2514 3306 156
Size: 691 577 122 778 190 792 1382
Fragments arranged by size:
1382 792 778 691 577 190 122
BsmI GAATG_Cn'
Cuts at: 1363 1363
Size: 4532
Bsp1286I G_dGCh'C
Cuts at: 156 457 847 1190 1424 1546 2157 2250 2324
Size: 301 390 343 234 122 611 93 74
Cuts at: 2324 2514 2576 3306 156
Size: 190 62 730 1382
Fragments arranged by size:
1382 730 611 390 343 301 234 190
122 93 74 62
BspEI T'CCGG_A
Cuts at: 461 830 3752 461
Size: 369 2922 1241
Fragments arranged by size:
2922 1241 369
BspHI T'CATG_A
Cuts at: 389 3708 389
Size: 3319 1213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 165 1150 1582 1796 2098 2479 165
Size: 985 432 214 302 381 2218
Fragments arranged by size:
2218 985 432 381 302 214
BsrBI CCG'CTC
Cuts at: 50 539 2824 2878 4362 4527 50
Size: 489 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 489 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2444 2444
Size: 4532
BsrFI r'CCGG_y
Cuts at: 1130 2530 2711 2920 1130
Size: 1400 181 209 2742
Fragments arranged by size:
2742 1400 209 181
BsrGI T'GTAC_A
Cuts at: 267 267
Size: 4532
BssSI C'ACGA_G
Cuts at: 2803 3161 2803
Size: 358 4174
BstAPI GCAn_nnn'nTGC
Cuts at: 767 1909 767
Size: 1142 3390
BstBI TT'CG_AA
Cuts at: 2893 2893
Size: 4532
BstYI r'GATC_y
Cuts at: 1 837 929 1040 1557 2024 2047 2382 2628
Size: 836 92 111 517 467 23 335 246
Cuts at: 2628 3640 3726 3738 4084 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 836 517 467 449 346 335 246
111 92 86 23 12
BtgI C'CryG_G
Cuts at: 575 575
Size: 4532
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2482 2663 2826 2482
Size: 181 163 4188
Fragments arranged by size:
4188 181 163
BtsI GCAGTG_nn'
Cuts at: 768 891 1154 1775 768
Size: 123 263 621 3525
Fragments arranged by size:
3525 621 263 123
DraI TTT'AAA
Cuts at: 1531 1531
Size: 4532
DraIII CAC_nnn'GTG
Cuts at: 1305 1305
Size: 4532
DrdI GACnn_nn'nnGTC
Cuts at: 2238 3096 2238
Size: 858 3674
EaeI y'GGCC_r
Cuts at: 1128 2117 2291 2682 2709 4054 1128
Size: 989 174 391 27 1345 1606
Fragments arranged by size:
1606 1345 989 391 174 27
EagI C'GGCC_G
Cuts at: 2117 2117
Size: 4532
EarI CTCTTCn'nnn_
Cuts at: 1273 2555 2765 1273
Size: 1282 210 3040
Fragments arranged by size:
3040 1282 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2740 3050 3196 4342 2740
Size: 310 146 1146 2930
Fragments arranged by size:
2930 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1422 1544 1422
Size: 122 4410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 553 999 1072 1204 1234 1363 1417 2357
Size: 306 446 73 132 30 129 54 940
Cuts at: 2357 2789 3536 3804 247
Size: 432 747 268 975
Fragments arranged by size:
975 940 747 446 432 306 268 132
129 73 54 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1014 1051 1498 1014
Size: 37 447 4048
Fragments arranged by size:
4048 447 37
EcoO109I rG'GnC_Cy
Cuts at: 143 334 792 1671 143
Size: 191 458 879 3004
Fragments arranged by size:
3004 879 458 191
EcoRI G'AATT_C
Cuts at: 1536 1536
Size: 4532
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4532
FspI TGC'GCA
Cuts at: 523 1462 2313 523
Size: 939 851 2742
Fragments arranged by size:
2742 939 851
HaeII r_GCGC'y
Cuts at: 473 1966 2214 3236 4215 473
Size: 1493 248 1022 979 790
Fragments arranged by size:
1493 1022 979 790 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 612 644 678 710 829 861 1027
Size: 32 309 32 34 32 119 32 166
Cuts at: 1027 1059 2374 2404 2406 2436 271
Size: 32 1315 30 2 30 2367
Fragments arranged by size:
2367 1315 309 166 119 34 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 312 965 1078 1571 312
Size: 653 113 493 3273
Fragments arranged by size:
3273 653 493 113
KasI G'GCGC_C
Cuts at: 469 1962 2210 469
Size: 1493 248 2791
Fragments arranged by size:
2791 1493 248
KpnI G_GTAC'C
Cuts at: 1552 1552
Size: 4532
MluI A'CGCG_T
Cuts at: 3965 3965
Size: 4532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 309 590 3203 3387 309
Size: 281 2613 184 1454
Fragments arranged by size:
2613 1454 281 184
MscI TGG'CCA
Cuts at: 2293 2293
Size: 4532
MslI CAynn'nnrTG
Cuts at: 383 1408 2521 2648 383
Size: 1025 1113 127 2267
Fragments arranged by size:
2267 1113 1025 127
MspA1I CmG'CkG
Cuts at: 666 867 1227 1317 1452 1643 1957 2317 3330
Size: 201 360 90 135 191 314 360 1013
Cuts at: 3330 3575 666
Size: 245 1623
Fragments arranged by size:
1623 1013 360 360 314 245 201 191
135 90
NaeI GCC'GGC
Cuts at: 1132 2713 1132
Size: 1581 2951
NarI GG'CG_CC
Cuts at: 470 1963 2211 470
Size: 1493 248 2791
Fragments arranged by size:
2791 1493 248
NgoMIV G'CCGG_C
Cuts at: 1130 2711 1130
Size: 1581 2951
NspI r_CATG'y
Cuts at: 398 963 975 1212 1585 2085 2992 398
Size: 565 12 237 373 500 907 1938
Fragments arranged by size:
1938 907 565 500 373 237 12
PciI A'CATG_T
Cuts at: 394 959 971 2988 394
Size: 565 12 2017 1938
Fragments arranged by size:
2017 1938 565 12
PfoI T'CCnGG_A
Cuts at: 294 1466 294
Size: 1172 3360
PmeI GTTT'AAAC
Cuts at: 1531 1531
Size: 4532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3671 3703 3671
Size: 32 4500
PpuMI rG'GwC_Cy
Cuts at: 143 334 143
Size: 191 4341
PstI C_TGCA'G
Cuts at: 791 866 1161 1226 1298 1322 1579 2264 791
Size: 75 295 65 72 24 257 685 3059
Fragments arranged by size:
3059 685 295 257 75 72 65 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4532
PvuII CAG'CTG
Cuts at: 867 1227 1317 1957 2317 867
Size: 360 90 640 360 3082
Fragments arranged by size:
3082 640 360 360 90
RsrII CG'GwC_CG
Cuts at: 2727 2727
Size: 4532
SacI G_AGCT'C
Cuts at: 1424 1546 1424
Size: 122 4410
SalI G'TCGA_C
Cuts at: 1569 1569
Size: 4532
SanDI GG'GwC_CC
Cuts at: 143 143
Size: 4532
SapI GCTCTTCn'nnn_
Cuts at: 2555 2765 2555
Size: 210 4322
SbfI CC_TGCA'GG
Cuts at: 1579 1579
Size: 4532
ScaI AGT'ACT
Cuts at: 4049 4049
Size: 4532
SfcI C'TryA_G
Cuts at: 33 787 862 1157 1222 1294 1318 1575 2260
Size: 754 75 295 65 72 24 257 685
Cuts at: 2260 3253 3444 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 754 685 295 257 191 75
72 65 24
SfoI GGC'GCC
Cuts at: 471 1964 2212 471
Size: 1493 248 2791
Fragments arranged by size:
2791 1493 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4532
SmaI CCC'GGG
Cuts at: 1554 1554
Size: 4532
SmlI C'TyrA_G
Cuts at: 1687 2602 3094 3356 1687
Size: 915 492 262 2863
Fragments arranged by size:
2863 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4532
SphI G_CATG'C
Cuts at: 1585 2085 1585
Size: 500 4032
StuI AGG'CCT
Cuts at: 511 511
Size: 4532
StyI C'CwwG_G
Cuts at: 1666 2020 1666
Size: 354 4178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1462 2115 2248 2707 2916 1462
Size: 653 133 459 209 3078
Fragments arranged by size:
3078 653 459 209 133
TatI w'GTAC_w
Cuts at: 267 4047 267
Size: 3780 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 597 629 4049 4081 597
Size: 32 3420 32 1048
Fragments arranged by size:
3420 1048 32 32
Tth111I GACn'n_nGTC
Cuts at: 2329 2329
Size: 4532
XbaI T'CTAG_A
Cuts at: 1563 1563
Size: 4532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2027 2027
Size: 4532
XmaI C'CCGG_G
Cuts at: 1552 1552
Size: 4532
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI