Restriction map for
pF1KB7717
(Circular) (Six-base) MAPSORT of: pF1KB7717.seq Check: 6471 from: 1 to: 4388
pF1KB7717 4388 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 515 720 2213 2645 3392 3660 398
Size: 117 205 1493 432 747 268 1126
Fragments arranged by size:
1493 1126 747 432 268 205 117
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AhdI GACnn_n'nnGTC
Cuts at: 831 831
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 638 1693 3260 638
Size: 1055 1567 1766
Fragments arranged by size:
1766 1567 1055
ApaLI G'TGCA_C
Cuts at: 3158 3158
Size: 4388
ApoI r'AATT_y
Cuts at: 478 602 854 938 1392 3741 3997 478
Size: 124 252 84 454 2349 256 869
Fragments arranged by size:
2349 869 454 256 252 124 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 560 1408 560
Size: 848 3540
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 1250 1404 1818 2066 2101 1250
Size: 154 414 248 35 3537
Fragments arranged by size:
3537 414 248 154 35
BanII G_rGCy'C
Cuts at: 790 1402 2432 790
Size: 612 1030 2746
Fragments arranged by size:
2746 1030 612
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 164 652 164
Size: 488 3900
BbvCI CC'TCA_GC
Cuts at: 754 754
Size: 4388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 145 179 145
Size: 34 4354
BciVI GTATCCnnnnn_n'
Cuts at: 2286 3047 4388 2286
Size: 761 1341 2286
Fragments arranged by size:
2286 1341 761
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglII A'GATC_T
Cuts at: 333 1903 3940 333
Size: 1570 2037 781
Fragments arranged by size:
2037 1570 781
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 2013 2106 3162 2013
Size: 93 1056 3239
Fragments arranged by size:
3239 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 393 704 788 1600 1681 2016 4160 393
Size: 311 84 812 81 335 2144 621
Fragments arranged by size:
2144 812 621 335 311 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 390 392 2755 390
Size: 2 2363 2023
Fragments arranged by size:
2363 2023 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 884 968 1220 259
Size: 625 84 252 3427
Fragments arranged by size:
3427 625 252 84
Bpu10I CC'TnA_GC
Cuts at: 754 754
Size: 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1564 2443 2935 3233 1564
Size: 879 492 298 2719
Fragments arranged by size:
2719 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4092 4092
Size: 4388
BsaAI yAC'GTr
Cuts at: 1741 1741
Size: 4388
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 364
Size: 4388
BseYI C'CCAG_C
Cuts at: 1114 1813 2571 3148 3644 1114
Size: 699 758 577 496 1858
Fragments arranged by size:
1858 758 699 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 819 819
Size: 4388
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 811 1402 2180 2370 3162 811
Size: 591 778 190 792 2037
Fragments arranged by size:
2037 792 778 591 190
BsmI GAATG_Cn'
Cuts at: 451 634 1222 1231 1306 451
Size: 183 588 9 75 3533
Fragments arranged by size:
3533 588 183 75 9
BsmBI CGTCTCn'nnnn_
Cuts at: 1091 1091
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 790 811 1402 2013 2106 2180 2370 2432 3162
Size: 21 591 611 93 74 190 62 730
Cuts at: 3162 790
Size: 2016
Fragments arranged by size:
2016 730 611 591 190 93 74 62
21
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1438 1652 1954 2335 1438
Size: 214 302 381 3491
Fragments arranged by size:
3491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1166 2680 2734 4218 4383 50
Size: 1116 1514 54 1484 165 55
Fragments arranged by size:
1514 1484 1116 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 1253 2386 2567 2776 1253
Size: 1133 181 209 2865
Fragments arranged by size:
2865 1133 209 181
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 806 1226 1765 806
Size: 420 539 3429
Fragments arranged by size:
3429 539 420
BstBI TT'CG_AA
Cuts at: 155 2749 155
Size: 2594 1794
BstYI r'GATC_y
Cuts at: 1 333 1190 1413 1880 1903 2238 2484 3496
Size: 332 857 223 467 23 335 246 1012
Cuts at: 3496 3582 3594 3940 1
Size: 86 12 346 449
Fragments arranged by size:
1012 857 467 449 346 335 332 246
223 86 23 12
BtgI C'CryG_G
Cuts at: 107 167 322 107
Size: 60 155 4173
Fragments arranged by size:
4173 155 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 639 807 1631 639
Size: 168 824 3396
Fragments arranged by size:
3396 824 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 873 908 873
Size: 35 4353
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DraIII CAC_nnn'GTG
Cuts at: 1310 1310
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 361 1973 2147 2538 2565 3910 361
Size: 1612 174 391 27 1345 839
Fragments arranged by size:
1612 1345 839 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 179 301 2411 2621 179
Size: 122 2110 210 1946
Fragments arranged by size:
2110 1946 210 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 2906 3052 4198 2596
Size: 310 146 1146 2786
Fragments arranged by size:
2786 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 398 515 720 884 968 1220 2213 2645
Size: 139 117 205 164 84 252 993 432
Cuts at: 2645 3392 3660 259
Size: 747 268 987
Fragments arranged by size:
993 987 747 432 268 252 205 164
139 117 84
EcoNI CCTnn'n_nnAGG
Cuts at: 223 223
Size: 4388
EcoO109I rG'GnC_Cy
Cuts at: 216 1119 1527 216
Size: 903 408 3077
Fragments arranged by size:
3077 903 408
EcoRI G'AATT_C
Cuts at: 854 938 1392 854
Size: 84 454 3850
Fragments arranged by size:
3850 454 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 1822 2070 3092 4071 1822
Size: 248 1022 979 2139
Fragments arranged by size:
2139 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 318 350 409 441 649 681 817 849 901
Size: 32 59 32 208 32 136 32 52
Cuts at: 901 933 1153 1185 2230 2260 2262 2292 318
Size: 32 220 32 1045 30 2 30 2414
Fragments arranged by size:
2414 1045 220 208 136 59 52 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
HindIII A'AGCT_T
Cuts at: 1067 1151 1067
Size: 84 4304
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MfeI C'AATT_G
Cuts at: 301 301
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 148 3059 3243 148
Size: 2911 184 1293
Fragments arranged by size:
2911 1293 184
MscI TGG'CCA
Cuts at: 363 2149 363
Size: 1786 2602
MslI CAynn'nnrTG
Cuts at: 974 1215 1271 2377 2504 974
Size: 241 56 1106 127 2858
Fragments arranged by size:
2858 1106 241 127 56
MspA1I CmG'CkG
Cuts at: 1248 1499 1813 2173 3186 3431 1248
Size: 251 314 360 1013 245 2205
Fragments arranged by size:
2205 1013 360 314 251 245
NaeI GCC'GGC
Cuts at: 1255 2569 1255
Size: 1314 3074
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NdeI CA'TA_TG
Cuts at: 1212 1212
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 1253 2567 1253
Size: 1314 3074
NspI r_CATG'y
Cuts at: 1441 1941 2848 1441
Size: 500 907 2981
Fragments arranged by size:
2981 907 500
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PpuMI rG'GwC_Cy
Cuts at: 216 1119 216
Size: 903 3485
PstI C_TGCA'G
Cuts at: 1435 2120 1435
Size: 685 3703
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 1813 2173 1813
Size: 360 4028
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SanDI GG'GwC_CC
Cuts at: 1119 1119
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 1431 2116 3109 3300 33
Size: 1398 685 993 191 1121
Fragments arranged by size:
1398 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 562 1410 562
Size: 848 3540
SmlI C'TyrA_G
Cuts at: 1543 2458 2950 3212 1543
Size: 915 492 262 2719
Fragments arranged by size:
2719 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
StyI C'CwwG_G
Cuts at: 107 450 1522 1876 107
Size: 343 1072 354 2619
Fragments arranged by size:
2619 1072 354 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 510 1971 2104 2563 2772 510
Size: 1461 133 459 209 2126
Fragments arranged by size:
2126 1461 459 209 133
TatI w'GTAC_w
Cuts at: 3903 3903
Size: 4388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 307 339 3905 3937 307
Size: 32 3566 32 758
Fragments arranged by size:
3566 758 32 32
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 378 1419 378
Size: 1041 3347
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 504 1883 504
Size: 1379 3009
XmaI C'CCGG_G
Cuts at: 560 1408 560
Size: 848 3540
XmnI GAAnn'nnTTC
Cuts at: 733 1069 1321 733
Size: 336 252 3800
Fragments arranged by size:
3800 336 252
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
EcoRV FseI FspAI HpaI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI