Restriction map for
pF1KB7728
(Circular) (Six-base) MAPSORT of: pF1KB7728.seq Check: 5095 from: 1 to: 4550
pF1KB7728 4550 bp
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 737 1028 737
Size: 291 4259
AccI GT'mk_AC
Cuts at: 439 1403 1588 439
Size: 964 185 3401
Fragments arranged by size:
3401 964 185
Acc65I G'GTAC_C
Cuts at: 1566 1566
Size: 4550
AclI AA'CG_TT
Cuts at: 4390 4390
Size: 4550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 2375 2807 3554 3822 1144
Size: 1231 432 747 268 1872
Fragments arranged by size:
1872 1231 747 432 268
AflIII A'CryG_T
Cuts at: 689 1902 3006 3983 689
Size: 1213 1104 977 1256
Fragments arranged by size:
1256 1213 1104 977
AgeI A'CCGG_T
Cuts at: 2938 2938
Size: 4550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 4550
AlwNI CAG_nnn'CTG
Cuts at: 456 1114 1242 1855 3422 456
Size: 658 128 613 1567 1584
Fragments arranged by size:
1584 1567 658 613 128
ApaI G_GGCC'C
Cuts at: 1417 1417
Size: 4550
ApaLI G'TGCA_C
Cuts at: 3320 3320
Size: 4550
ApoI r'AATT_y
Cuts at: 631 1554 3903 4159 631
Size: 923 2349 256 1022
Fragments arranged by size:
2349 1022 923 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4550
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
AvaI C'yCGr_G
Cuts at: 130 220 1133 1570 130
Size: 90 913 437 3110
Fragments arranged by size:
3110 913 437 90
BamHI G'GATC_C
Cuts at: 491 1172 1575 491
Size: 681 403 3466
Fragments arranged by size:
3466 681 403
BanI G'GyrC_C
Cuts at: 1566 1980 2228 2263 1566
Size: 414 248 35 3853
Fragments arranged by size:
3853 414 248 35
BanII G_rGCy'C
Cuts at: 1417 1564 2594 1417
Size: 147 1030 3373
Fragments arranged by size:
3373 1030 147
BbeI G_GCGC'C
Cuts at: 1984 2232 1984
Size: 248 4302
BbsI GAAGACnn'nnnn_
Cuts at: 408 796 408
Size: 388 4162
BbvCI CC'TCA_GC
Cuts at: 1486 1486
Size: 4550
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4516
BciVI GTATCCnnnnn_n'
Cuts at: 2448 3209 4550 2448
Size: 761 1341 2448
Fragments arranged by size:
2448 1341 761
BclI T'GATC_A
Cuts at: 2070 2070
Size: 4550
BglI GCCn_nnn'nGGC
Cuts at: 1421 1421
Size: 4550
BglII A'GATC_T
Cuts at: 375 1151 2065 4102 375
Size: 776 914 2037 823
Fragments arranged by size:
2037 914 823 776
BlpI GC'TnA_GC
Cuts at: 1662 1662
Size: 4550
Bme1580I G_kGCm'C
Cuts at: 671 1262 1417 2175 2268 3324 671
Size: 591 155 758 93 1056 1897
Fragments arranged by size:
1897 1056 758 591 155 93
BmgBI CAC'GTC
Cuts at: 272 894 272
Size: 622 3928
BmrI ACTGGGnnnn_n'
Cuts at: 1762 1843 2178 4322 1762
Size: 81 335 2144 1990
Fragments arranged by size:
2144 1990 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4550
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 478 1264 367
Size: 111 786 3653
Fragments arranged by size:
3653 786 111
Bpu10I CC'TnA_GC
Cuts at: 1486 1486
Size: 4550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1726 2605 3097 3395 1726
Size: 879 492 298 2881
Fragments arranged by size:
2881 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4254 4254
Size: 4550
BsaAI yAC'GTr
Cuts at: 1903 1903
Size: 4550
BsaBI GATnn'nnATC
Cuts at: 6 2064 4101 6
Size: 2058 2037 455
Fragments arranged by size:
2058 2037 455
BsaHI Gr'CG_yC
Cuts at: 1981 2229 2931 4427 1981
Size: 248 702 1496 2104
Fragments arranged by size:
2104 1496 702 248
BsaWI w'CCGG_w
Cuts at: 488 1739 2260 2938 3212 3359 3770 488
Size: 1251 521 678 274 147 411 1268
Fragments arranged by size:
1268 1251 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1363 1393 1363
Size: 30 4520
BseYI C'CCAG_C
Cuts at: 209 276 360 551 1049 1385 1975 2733 3310
Size: 67 84 191 498 336 590 758 577
Cuts at: 3310 3806 209
Size: 496 953
Fragments arranged by size:
953 758 590 577 498 496 336 191
84 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 715 1474 715
Size: 759 3791
BsiEI CG_ry'CG
Cuts at: 104 780 2092 2138 3346 104
Size: 676 1312 46 1208 1308
Fragments arranged by size:
1312 1308 1208 676 46
BsiHKAI G_wGCw'C
Cuts at: 137 179 449 728 880 1564 2342 2532 3324
Size: 42 270 279 152 684 778 190 792
Cuts at: 3324 137
Size: 1363
Fragments arranged by size:
1363 792 778 684 279 270 190 152
42
BsmI GAATG_Cn'
Cuts at: 564 601 564
Size: 37 4513
BsmBI CGTCTCn'nnnn_
Cuts at: 292 292
Size: 4550
Bsp1286I G_dGCh'C
Cuts at: 137 179 449 671 728 880 1262 1417 1564
Size: 42 270 222 57 152 382 155 147
Cuts at: 1564 2175 2268 2342 2532 2594 3324 137
Size: 611 93 74 190 62 730 1363
Fragments arranged by size:
1363 730 611 382 270 222 190 155
152 147 93 74 62 57 42
BspEI T'CCGG_A
Cuts at: 3770 3770
Size: 4550
BspHI T'CATG_A
Cuts at: 3726 3726
Size: 4550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 173 737 1028 1600 1814 2116 2497 173
Size: 564 291 572 214 302 381 2226
Fragments arranged by size:
2226 572 564 381 302 291 214
BsrBI CCG'CTC
Cuts at: 50 2842 2896 4380 4545 50
Size: 2792 54 1484 165 55
Fragments arranged by size:
2792 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1515 2462 1515
Size: 947 3603
BsrFI r'CCGG_y
Cuts at: 428 1088 1406 2548 2729 2938 428
Size: 660 318 1142 181 209 2040
Fragments arranged by size:
2040 1142 660 318 209 181
BsrGI T'GTAC_A
Cuts at: 1370 1370
Size: 4550
BssSI C'ACGA_G
Cuts at: 2821 3179 2821
Size: 358 4192
BstAPI GCAn_nnn'nTGC
Cuts at: 1927 1927
Size: 4550
BstBI TT'CG_AA
Cuts at: 2911 2911
Size: 4550
BstXI CCAn_nnnn'nTGG
Cuts at: 1156 1323 1156
Size: 167 4383
BstYI r'GATC_y
Cuts at: 1 375 491 1151 1172 1575 2042 2065 2400
Size: 374 116 660 21 403 467 23 335
Cuts at: 2400 2646 3658 3744 3756 4102 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 660 467 449 403 374 346 335
246 116 86 23 21 12
BstZ17I GTA'TAC
Cuts at: 440 440
Size: 4550
BtgI C'CryG_G
Cuts at: 883 1004 1418 883
Size: 121 414 4015
Fragments arranged by size:
4015 414 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2500 2681 2844 2500
Size: 181 163 4206
Fragments arranged by size:
4206 181 163
BtsI GCAGTG_nn'
Cuts at: 615 1017 1060 1290 1793 615
Size: 402 43 230 503 3372
Fragments arranged by size:
3372 503 402 230 43
ClaI AT'CG_AT
Cuts at: 503 503
Size: 4550
DraI TTT'AAA
Cuts at: 1549 1549
Size: 4550
DraIII CAC_nnn'GTG
Cuts at: 875 990 875
Size: 115 4435
DrdI GACnn_nn'nnGTC
Cuts at: 2256 3114 2256
Size: 858 3692
EaeI y'GGCC_r
Cuts at: 478 2135 2309 2700 2727 4072 478
Size: 1657 174 391 27 1345 956
Fragments arranged by size:
1657 1345 956 391 174 27
EagI C'GGCC_G
Cuts at: 2135 2135
Size: 4550
EarI CTCTTCn'nnn_
Cuts at: 118 290 583 1186 1322 2573 2783 118
Size: 172 293 603 136 1251 210 1885
Fragments arranged by size:
1885 1251 603 293 210 172 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2758 3068 3214 4360 2758
Size: 310 146 1146 2948
Fragments arranged by size:
2948 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1562 1562
Size: 4550
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 478 1144 1264 2375 2807 3554 3822 367
Size: 111 666 120 1111 432 747 268 1095
Fragments arranged by size:
1111 1095 747 666 432 268 120 111
EcoO109I rG'GnC_Cy
Cuts at: 579 996 1413 1414 1689 579
Size: 417 417 1 275 3440
Fragments arranged by size:
3440 417 417 275 1
EcoRI G'AATT_C
Cuts at: 631 1554 631
Size: 923 3627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2569 2569
Size: 4550
FspI TGC'GCA
Cuts at: 2331 2331
Size: 4550
HaeII r_GCGC'y
Cuts at: 917 1984 2232 3254 4233 917
Size: 1067 248 1022 979 1234
Fragments arranged by size:
1234 1067 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1143 1175 2392 2422 2424 2454 1143
Size: 32 1217 30 2 30 3239
Fragments arranged by size:
3239 1217 32 30 30 2
HincII GTy'rAC
Cuts at: 1589 1589
Size: 4550
KasI G'GCGC_C
Cuts at: 1980 2228 1980
Size: 248 4302
KpnI G_GTAC'C
Cuts at: 1570 1570
Size: 4550
MluI A'CGCG_T
Cuts at: 3983 3983
Size: 4550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 363 1003 3221 3405 363
Size: 640 2218 184 1508
Fragments arranged by size:
2218 1508 640 184
MscI TGG'CCA
Cuts at: 2311 2311
Size: 4550
MslI CAynn'nnrTG
Cuts at: 409 1429 2539 2666 409
Size: 1020 1110 127 2293
Fragments arranged by size:
2293 1110 1020 127
MspA1I CmG'CkG
Cuts at: 213 456 555 743 1661 1975 2335 3348 3593
Size: 243 99 188 918 314 360 1013 245
Cuts at: 3593 213
Size: 1170
Fragments arranged by size:
1170 1013 918 360 314 245 243 188
99
NaeI GCC'GGC
Cuts at: 430 1090 2731 430
Size: 660 1641 2249
Fragments arranged by size:
2249 1641 660
NarI GG'CG_CC
Cuts at: 1981 2229 1981
Size: 248 4302
NcoI C'CATG_G
Cuts at: 883 883
Size: 4550
NgoMIV G'CCGG_C
Cuts at: 428 1088 2729 428
Size: 660 1641 2249
Fragments arranged by size:
2249 1641 660
NspI r_CATG'y
Cuts at: 1603 2103 3010 1603
Size: 500 907 3143
Fragments arranged by size:
3143 907 500
PasI CC'CwG_GG
Cuts at: 536 536
Size: 4550
PciI A'CATG_T
Cuts at: 3006 3006
Size: 4550
PflMI CCAn_nnn'nTGG
Cuts at: 456 990 1434 456
Size: 534 444 3572
Fragments arranged by size:
3572 534 444
PfoI T'CCnGG_A
Cuts at: 494 1040 1322 494
Size: 546 282 3722
Fragments arranged by size:
3722 546 282
PmeI GTTT'AAAC
Cuts at: 1549 1549
Size: 4550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4518
PpuMI rG'GwC_Cy
Cuts at: 579 579
Size: 4550
PspOMI G'GGCC_C
Cuts at: 1413 1413
Size: 4550
PstI C_TGCA'G
Cuts at: 437 1268 1597 2282 437
Size: 831 329 685 2705
Fragments arranged by size:
2705 831 685 329
PvuI CG_AT'CG
Cuts at: 104 780 104
Size: 676 3874
PvuII CAG'CTG
Cuts at: 213 456 555 743 1975 2335 213
Size: 243 99 188 1232 360 2428
Fragments arranged by size:
2428 1232 360 243 188 99
RsrII CG'GwC_CG
Cuts at: 2745 2745
Size: 4550
SacI G_AGCT'C
Cuts at: 1564 1564
Size: 4550
SalI G'TCGA_C
Cuts at: 1587 1587
Size: 4550
SanDI GG'GwC_CC
Cuts at: 579 579
Size: 4550
SapI GCTCTTCn'nnn_
Cuts at: 118 1186 1322 2573 2783 118
Size: 1068 136 1251 210 1885
Fragments arranged by size:
1885 1251 1068 210 136
SbfI CC_TGCA'GG
Cuts at: 1597 1597
Size: 4550
ScaI AGT'ACT
Cuts at: 4067 4067
Size: 4550
SexAI A'CCwGG_T
Cuts at: 902 902
Size: 4550
SfcI C'TryA_G
Cuts at: 33 433 1264 1593 2278 3271 3462 33
Size: 400 831 329 685 993 191 1121
Fragments arranged by size:
1121 993 831 685 400 329 191
SfoI GGC'GCC
Cuts at: 1982 2230 1982
Size: 248 4302
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4550
SmaI CCC'GGG
Cuts at: 1572 1572
Size: 4550
SmlI C'TyrA_G
Cuts at: 1705 2620 3112 3374 1705
Size: 915 492 262 2881
Fragments arranged by size:
2881 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4550
SphI G_CATG'C
Cuts at: 1603 2103 1603
Size: 500 4050
StyI C'CwwG_G
Cuts at: 318 883 1340 1684 2038 318
Size: 565 457 344 354 2830
Fragments arranged by size:
2830 565 457 354 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2133 2266 2725 2934 2133
Size: 133 459 209 3749
Fragments arranged by size:
3749 459 209 133
TatI w'GTAC_w
Cuts at: 716 1370 4065 716
Size: 654 2695 1201
Fragments arranged by size:
2695 1201 654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4067 4099 4067
Size: 32 4518
Tth111I GACn'n_nGTC
Cuts at: 2347 2347
Size: 4550
XbaI T'CTAG_A
Cuts at: 1581 1581
Size: 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2045 2045
Size: 4550
XmaI C'CCGG_G
Cuts at: 1570 1570
Size: 4550
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmtI BseRI BsiWI BssHII BstEII Bsu36I
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI