Restriction map for
pF1KB7738
(Circular) (Six-base) MAPSORT of: pF1KB7738.seq Check: 672 from: 1 to: 4565
pF1KB7738 4565 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1603 1603
Size: 4565
Acc65I G'GTAC_C
Cuts at: 315 635 860 1581 315
Size: 320 225 721 3299
Fragments arranged by size:
3299 721 320 225
AclI AA'CG_TT
Cuts at: 4405 4405
Size: 4565
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 278 326 2390 2822 3569 3837 144
Size: 134 48 2064 432 747 268 872
Fragments arranged by size:
2064 872 747 432 268 134 48
AfeI AGC'GCT
Cuts at: 200 200
Size: 4565
AflIII A'CryG_T
Cuts at: 1355 1917 3021 3998 1355
Size: 562 1104 977 1922
Fragments arranged by size:
1922 1104 977 562
AgeI A'CCGG_T
Cuts at: 2953 2953
Size: 4565
AlwNI CAG_nnn'CTG
Cuts at: 672 1247 1870 3437 672
Size: 575 623 1567 1800
Fragments arranged by size:
1800 1567 623 575
ApaI G_GGCC'C
Cuts at: 971 971
Size: 4565
ApaLI G'TGCA_C
Cuts at: 3335 3335
Size: 4565
ApoI r'AATT_y
Cuts at: 588 1054 1569 3918 4174 588
Size: 466 515 2349 256 979
Fragments arranged by size:
2349 979 515 466 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4565
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4565
AvaI C'yCGr_G
Cuts at: 231 572 640 1378 1585 231
Size: 341 68 738 207 3211
Fragments arranged by size:
3211 738 341 207 68
BamHI G'GATC_C
Cuts at: 1590 1590
Size: 4565
BanI G'GyrC_C
Cuts at: 315 538 607 635 860 1123 1581 1995 2243
Size: 223 69 28 225 263 458 414 248
Cuts at: 2243 2278 315
Size: 35 2602
Fragments arranged by size:
2602 458 414 263 248 225 223 69
35 28
BanII G_rGCy'C
Cuts at: 238 404 755 971 1379 1495 1579 2609 238
Size: 166 351 216 408 116 84 1030 2194
Fragments arranged by size:
2194 1030 408 351 216 166 116 84
BbeI G_GCGC'C
Cuts at: 1999 2247 1999
Size: 248 4317
BbsI GAAGACnn'nnnn_
Cuts at: 651 651
Size: 4565
BciVI GTATCCnnnnn_n'
Cuts at: 124 2463 3224 4565 124
Size: 2339 761 1341 124
Fragments arranged by size:
2339 1341 761 124
BclI T'GATC_A
Cuts at: 881 2085 881
Size: 1204 3361
BglII A'GATC_T
Cuts at: 261 2080 4117 261
Size: 1819 2037 709
Fragments arranged by size:
2037 1819 709
BlpI GC'TnA_GC
Cuts at: 1291 1677 1291
Size: 386 4179
Bme1580I G_kGCm'C
Cuts at: 152 543 857 971 1128 2190 2283 3339 152
Size: 391 314 114 157 1062 93 1056 1378
Fragments arranged by size:
1378 1062 1056 391 314 157 114 93
BmgBI CAC'GTC
Cuts at: 296 296
Size: 4565
BmrI ACTGGGnnnn_n'
Cuts at: 1777 1858 2193 4337 1777
Size: 81 335 2144 2005
Fragments arranged by size:
2144 2005 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 978 2932 978
Size: 1954 2611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 959 471
Size: 488 4077
Bpu10I CC'TnA_GC
Cuts at: 311 311
Size: 4565
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 1741 2620 3112 3410 1411
Size: 330 879 492 298 2566
Fragments arranged by size:
2566 879 492 330 298
BsaI GGTCTCn'nnnn_
Cuts at: 4269 4269
Size: 4565
BsaAI yAC'GTr
Cuts at: 1356 1918 1356
Size: 562 4003
BsaBI GATnn'nnATC
Cuts at: 6 2079 4116 6
Size: 2073 2037 455
Fragments arranged by size:
2073 2037 455
BsaHI Gr'CG_yC
Cuts at: 1996 2244 2946 4442 1996
Size: 248 702 1496 2119
Fragments arranged by size:
2119 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1754 2275 2953 3227 3374 3785 1754
Size: 521 678 274 147 411 2534
Fragments arranged by size:
2534 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 220 250 220
Size: 30 4535
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1246 1471 1246
Size: 225 4340
BseYI C'CCAG_C
Cuts at: 213 614 723 770 1526 1990 2748 3325 3821
Size: 401 109 47 756 464 758 577 496
Cuts at: 3821 213
Size: 957
Fragments arranged by size:
957 758 756 577 496 464 401 109
47
BsiEI CG_ry'CG
Cuts at: 104 2107 2153 3361 104
Size: 2003 46 1208 1308
Fragments arranged by size:
2003 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1579 2357 2547 3339 1579
Size: 778 190 792 2805
Fragments arranged by size:
2805 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 286 286
Size: 4565
Bsp1286I G_dGCh'C
Cuts at: 152 238 404 543 755 857 971 1128 1379
Size: 86 166 139 212 102 114 157 251
Cuts at: 1379 1495 1579 2190 2283 2357 2547 2609 3339
Size: 116 84 611 93 74 190 62 730
Cuts at: 3339 152
Size: 1378
Fragments arranged by size:
1378 730 611 251 212 190 166 157
139 116 114 102 93 86 84 74
62
BspEI T'CCGG_A
Cuts at: 3785 3785
Size: 4565
BspHI T'CATG_A
Cuts at: 3741 3741
Size: 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1615 1829 2131 2512 1615
Size: 214 302 381 3668
Fragments arranged by size:
3668 381 302 214
BsrBI CCG'CTC
Cuts at: 50 114 1298 2857 2911 4395 4560 50
Size: 64 1184 1559 54 1484 165 55
Fragments arranged by size:
1559 1484 1184 165 64 55 54
BsrDI GCAATG_nn'
Cuts at: 1184 2477 1184
Size: 1293 3272
BsrFI r'CCGG_y
Cuts at: 1265 1442 2563 2744 2953 1265
Size: 177 1121 181 209 2877
Fragments arranged by size:
2877 1121 209 181 177
BsrGI T'GTAC_A
Cuts at: 1358 1358
Size: 4565
BssSI C'ACGA_G
Cuts at: 2836 3194 2836
Size: 358 4207
BstAPI GCAn_nnn'nTGC
Cuts at: 1942 1942
Size: 4565
BstBI TT'CG_AA
Cuts at: 2926 2926
Size: 4565
BstXI CCAn_nnnn'nTGG
Cuts at: 1534 1534
Size: 4565
BstYI r'GATC_y
Cuts at: 1 261 273 598 1590 2057 2080 2415 2661
Size: 260 12 325 992 467 23 335 246
Cuts at: 2661 3673 3759 3771 4117 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 992 467 449 346 335 325 260
246 86 23 12 12
Bsu36I CC'TnA_GG
Cuts at: 1229 1229
Size: 4565
BtgI C'CryG_G
Cuts at: 107 465 630 830 989 107
Size: 358 165 200 159 3683
Fragments arranged by size:
3683 358 200 165 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2515 2696 2859 2515
Size: 181 163 4221
Fragments arranged by size:
4221 181 163
BtsI GCAGTG_nn'
Cuts at: 481 1808 481
Size: 1327 3238
DraI TTT'AAA
Cuts at: 1564 1564
Size: 4565
DraIII CAC_nnn'GTG
Cuts at: 929 929
Size: 4565
DrdI GACnn_nn'nnGTC
Cuts at: 919 1447 2271 3129 919
Size: 528 824 858 2355
Fragments arranged by size:
2355 858 824 528
EaeI y'GGCC_r
Cuts at: 627 1267 2150 2324 2715 2742 4087 627
Size: 640 883 174 391 27 1345 1105
Fragments arranged by size:
1345 1105 883 640 391 174 27
EagI C'GGCC_G
Cuts at: 2150 2150
Size: 4565
EarI CTCTTCn'nnn_
Cuts at: 271 1393 2588 2798 271
Size: 1122 1195 210 2038
Fragments arranged by size:
2038 1195 1122 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2773 3083 3229 4375 2773
Size: 310 146 1146 2963
Fragments arranged by size:
2963 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1577 1577
Size: 4565
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 278 326 471 959 2390 2822 3569 3837
Size: 134 48 145 488 1431 432 747 268
Cuts at: 3837 144
Size: 872
Fragments arranged by size:
1431 872 747 488 432 268 145 134
48
EcoNI CCTnn'n_nnAGG
Cuts at: 438 438
Size: 4565
EcoO109I rG'GnC_Cy
Cuts at: 968 1383 1704 968
Size: 415 321 3829
Fragments arranged by size:
3829 415 321
EcoRI G'AATT_C
Cuts at: 1569 1569
Size: 4565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4565
FspI TGC'GCA
Cuts at: 933 2346 933
Size: 1413 3152
FspAI rTGC'GCAy
Cuts at: 933 933
Size: 4565
HaeII r_GCGC'y
Cuts at: 202 1119 1999 2247 3269 4248 202
Size: 917 880 248 1022 979 519
Fragments arranged by size:
1022 979 917 880 519 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 2407 2437 2439 2469 220
Size: 32 2155 30 2 30 2316
Fragments arranged by size:
2316 2155 32 30 30 2
HincII GTy'rAC
Cuts at: 1604 1604
Size: 4565
KasI G'GCGC_C
Cuts at: 1995 2243 1995
Size: 248 4317
KpnI G_GTAC'C
Cuts at: 319 639 864 1585 319
Size: 320 225 721 3299
Fragments arranged by size:
3299 721 320 225
MluI A'CGCG_T
Cuts at: 3998 3998
Size: 4565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 728 3236 3420 728
Size: 2508 184 1873
Fragments arranged by size:
2508 1873 184
MscI TGG'CCA
Cuts at: 629 2326 629
Size: 1697 2868
MslI CAynn'nnrTG
Cuts at: 1484 2554 2681 1484
Size: 1070 127 3368
Fragments arranged by size:
3368 1070 127
MspA1I CmG'CkG
Cuts at: 213 744 1134 1676 1990 2350 3363 3608 213
Size: 531 390 542 314 360 1013 245 1170
Fragments arranged by size:
1170 1013 542 531 390 360 314 245
NaeI GCC'GGC
Cuts at: 2746 2746
Size: 4565
NarI GG'CG_CC
Cuts at: 1996 2244 1996
Size: 248 4317
NcoI C'CATG_G
Cuts at: 107 465 630 989 107
Size: 358 165 359 3683
Fragments arranged by size:
3683 359 358 165
NgoMIV G'CCGG_C
Cuts at: 2744 2744
Size: 4565
NspI r_CATG'y
Cuts at: 1618 2118 3025 1618
Size: 500 907 3158
Fragments arranged by size:
3158 907 500
PasI CC'CwG_GG
Cuts at: 865 865
Size: 4565
PciI A'CATG_T
Cuts at: 3021 3021
Size: 4565
PflMI CCAn_nnn'nTGG
Cuts at: 1247 1247
Size: 4565
PfoI T'CCnGG_A
Cuts at: 269 287 1403 269
Size: 18 1116 3431
Fragments arranged by size:
3431 1116 18
PmeI GTTT'AAAC
Cuts at: 1564 1564
Size: 4565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3704 3736 3704
Size: 32 4533
PspOMI G'GGCC_C
Cuts at: 967 967
Size: 4565
PstI C_TGCA'G
Cuts at: 439 1133 1612 2297 439
Size: 694 479 685 2707
Fragments arranged by size:
2707 694 685 479
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4565
PvuII CAG'CTG
Cuts at: 744 1134 1990 2350 744
Size: 390 856 360 2959
Fragments arranged by size:
2959 856 390 360
RsrII CG'GwC_CG
Cuts at: 2760 2760
Size: 4565
SacI G_AGCT'C
Cuts at: 1579 1579
Size: 4565
SalI G'TCGA_C
Cuts at: 1602 1602
Size: 4565
SapI GCTCTTCn'nnn_
Cuts at: 2588 2798 2588
Size: 210 4355
SbfI CC_TGCA'GG
Cuts at: 1612 1612
Size: 4565
ScaI AGT'ACT
Cuts at: 4082 4082
Size: 4565
SfcI C'TryA_G
Cuts at: 33 435 559 1129 1608 2293 3286 3477 33
Size: 402 124 570 479 685 993 191 1121
Fragments arranged by size:
1121 993 685 570 479 402 191 124
SfoI GGC'GCC
Cuts at: 1997 2245 1997
Size: 248 4317
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4565
SmaI CCC'GGG
Cuts at: 233 574 1587 233
Size: 341 1013 3211
Fragments arranged by size:
3211 1013 341
SmlI C'TyrA_G
Cuts at: 1426 1720 2635 3127 3389 1426
Size: 294 915 492 262 2602
Fragments arranged by size:
2602 915 492 294 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4565
SphI G_CATG'C
Cuts at: 1618 2118 1618
Size: 500 4065
StyI C'CwwG_G
Cuts at: 107 465 543 630 989 1541 1699 2053 107
Size: 358 78 87 359 552 158 354 2619
Fragments arranged by size:
2619 552 359 358 354 158 87 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2148 2281 2740 2949 2148
Size: 133 459 209 3764
Fragments arranged by size:
3764 459 209 133
TatI w'GTAC_w
Cuts at: 1358 4080 1358
Size: 2722 1843
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4082 4114 4082
Size: 32 4533
Tth111I GACn'n_nGTC
Cuts at: 1465 2362 1465
Size: 897 3668
XbaI T'CTAG_A
Cuts at: 1596 1596
Size: 4565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 622 628 2060 622
Size: 6 1432 3127
Fragments arranged by size:
3127 1432 6
XmaI C'CCGG_G
Cuts at: 231 572 1585 231
Size: 341 1013 3211
Fragments arranged by size:
3211 1013 341
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsgI
BsiWI BsmI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI