Restriction map for
pF1KB7740
(Circular) (Six-base) MAPSORT of: pF1KB7740.seq Check: 827 from: 1 to: 4559
pF1KB7740 4559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 684 1597 684
Size: 913 3646
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 4399 4399
Size: 4559
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 573 1342 2384 2816 3563 3831 389
Size: 184 769 1042 432 747 268 1117
Fragments arranged by size:
1117 1042 769 747 432 268 184
AflIII A'CryG_T
Cuts at: 1911 3015 3992 1911
Size: 1104 977 2478
Fragments arranged by size:
2478 1104 977
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 258 1864 3431 258
Size: 1606 1567 1386
Fragments arranged by size:
1606 1567 1386
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 737 953 967 984 1036 1190 1563 3912 4168
Size: 216 14 17 52 154 373 2349 256
Cuts at: 4168 737
Size: 1128
Fragments arranged by size:
2349 1128 373 256 216 154 52 17
14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 1575 1989 2237 2272 1575
Size: 414 248 35 3862
Fragments arranged by size:
3862 414 248 35
BanII G_rGCy'C
Cuts at: 1573 2603 1573
Size: 1030 3529
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BbsI GAAGACnn'nnnn_
Cuts at: 809 1085 809
Size: 276 4283
BciVI GTATCCnnnnn_n'
Cuts at: 538 2457 3218 4559 538
Size: 1919 761 1341 538
Fragments arranged by size:
1919 1341 761 538
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 1671 1671
Size: 4559
Bme1580I G_kGCm'C
Cuts at: 2184 2277 3333 2184
Size: 93 1056 3410
Fragments arranged by size:
3410 1056 93
BmgBI CAC'GTC
Cuts at: 1548 1548
Size: 4559
BmrI ACTGGGnnnn_n'
Cuts at: 1771 1852 2187 4331 1771
Size: 81 335 2144 1999
Fragments arranged by size:
2144 1999 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 396 950 2926 396
Size: 554 1976 2029
Fragments arranged by size:
2029 1976 554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1298 1298
Size: 4559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1735 2614 3106 3404 1256
Size: 479 879 492 298 2411
Fragments arranged by size:
2411 879 492 479 298
BsaI GGTCTCn'nnnn_
Cuts at: 4263 4263
Size: 4559
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 2073 4110 6
Size: 2067 2037 455
Fragments arranged by size:
2067 2037 455
BsaHI Gr'CG_yC
Cuts at: 1990 2238 2940 4436 1990
Size: 248 702 1496 2113
Fragments arranged by size:
2113 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1291 1291
Size: 4559
BseYI C'CCAG_C
Cuts at: 359 1984 2742 3319 3815 359
Size: 1625 758 577 496 1103
Fragments arranged by size:
1625 1103 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 995 1570 995
Size: 575 3984
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1573 2351 2541 3333 1573
Size: 778 190 792 2799
Fragments arranged by size:
2799 792 778 190
BsmI GAATG_Cn'
Cuts at: 727 1167 727
Size: 440 4119
Bsp1286I G_dGCh'C
Cuts at: 1573 2184 2277 2351 2541 2603 3333 1573
Size: 611 93 74 190 62 730 2799
Fragments arranged by size:
2799 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 3735 3735
Size: 4559
BspMI ACCTGCnnnn'nnnn_
Cuts at: 229 1542 1609 1823 2125 2506 229
Size: 1313 67 214 302 381 2282
Fragments arranged by size:
2282 1313 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2851 2905 4389 4554 50
Size: 2801 54 1484 165 55
Fragments arranged by size:
2801 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 294 2471 294
Size: 2177 2382
BsrFI r'CCGG_y
Cuts at: 2557 2738 2947 2557
Size: 181 209 4169
Fragments arranged by size:
4169 209 181
BsrGI T'GTAC_A
Cuts at: 617 656 1020 617
Size: 39 364 4156
Fragments arranged by size:
4156 364 39
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 1194 2920 1194
Size: 1726 2833
BstYI r'GATC_y
Cuts at: 1 1584 2051 2074 2409 2655 3667 3753 3765
Size: 1583 467 23 335 246 1012 86 12
Cuts at: 3765 4111 1
Size: 346 449
Fragments arranged by size:
1583 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 935 935
Size: 4559
BtgI C'CryG_G
Cuts at: 107 107
Size: 4559
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2509 2690 2853 2509
Size: 181 163 4215
Fragments arranged by size:
4215 181 163
BtsI GCAGTG_nn'
Cuts at: 156 1802 156
Size: 1646 2913
DraI TTT'AAA
Cuts at: 959 1558 959
Size: 599 3960
DrdI GACnn_nn'nnGTC
Cuts at: 846 2265 3123 846
Size: 1419 858 2282
Fragments arranged by size:
2282 1419 858
EaeI y'GGCC_r
Cuts at: 2144 2318 2709 2736 4081 2144
Size: 174 391 27 1345 2622
Fragments arranged by size:
2622 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 365 896 2582 2792 365
Size: 531 1686 210 2132
Fragments arranged by size:
2132 1686 531 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2767 3077 3223 4369 2767
Size: 310 146 1146 2957
Fragments arranged by size:
2957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 573 1298 1342 2384 2816 3563 3831 389
Size: 184 725 44 1042 432 747 268 1117
Fragments arranged by size:
1117 1042 747 725 432 268 184 44
EcoO109I rG'GnC_Cy
Cuts at: 241 1698 241
Size: 1457 3102
EcoRI G'AATT_C
Cuts at: 967 1036 1190 1563 967
Size: 69 154 373 3963
Fragments arranged by size:
3963 373 154 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4559
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 1993 2241 3263 4242 1993
Size: 248 1022 979 2310
Fragments arranged by size:
2310 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 1452 1484 2401 2431 2433 2463 523
Size: 32 897 32 917 30 2 30 2619
Fragments arranged by size:
2619 917 897 32 32 30 30 2
HincII GTy'rAC
Cuts at: 685 731 1598 685
Size: 46 867 3646
Fragments arranged by size:
3646 867 46
HindIII A'AGCT_T
Cuts at: 1354 1354
Size: 4559
HpaI GTT'AAC
Cuts at: 731 731
Size: 4559
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MfeI C'AATT_G
Cuts at: 1298 1298
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 365 3230 3414 365
Size: 2865 184 1510
Fragments arranged by size:
2865 1510 184
MscI TGG'CCA
Cuts at: 2320 2320
Size: 4559
MslI CAynn'nnrTG
Cuts at: 112 190 554 2548 2675 112
Size: 78 364 1994 127 1996
Fragments arranged by size:
1996 1994 364 127 78
MspA1I CmG'CkG
Cuts at: 164 415 1135 1670 1984 2344 3357 3602 164
Size: 251 720 535 314 360 1013 245 1121
Fragments arranged by size:
1121 1013 720 535 360 314 251 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NcoI C'CATG_G
Cuts at: 107 107
Size: 4559
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NspI r_CATG'y
Cuts at: 663 784 1612 2112 3019 663
Size: 121 828 500 907 2203
Fragments arranged by size:
2203 907 828 500 121
PciI A'CATG_T
Cuts at: 3015 3015
Size: 4559
PfoI T'CCnGG_A
Cuts at: 384 384
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3698 3730 3698
Size: 32 4527
PpuMI rG'GwC_Cy
Cuts at: 241 241
Size: 4559
PsiI TTA'TAA
Cuts at: 1244 1244
Size: 4559
PstI C_TGCA'G
Cuts at: 163 1606 2291 163
Size: 1443 685 2431
Fragments arranged by size:
2431 1443 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 164 415 1135 1984 2344 164
Size: 251 720 849 360 2379
Fragments arranged by size:
2379 849 720 360 251
RsrII CG'GwC_CG
Cuts at: 2754 2754
Size: 4559
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SalI G'TCGA_C
Cuts at: 683 1596 683
Size: 913 3646
SapI GCTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 4076 4076
Size: 4559
SfcI C'TryA_G
Cuts at: 33 159 1602 2287 3280 3471 33
Size: 126 1443 685 993 191 1121
Fragments arranged by size:
1443 1121 993 685 191 126
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 1271 1714 2629 3121 3383 1271
Size: 443 915 492 262 2447
Fragments arranged by size:
2447 915 492 443 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 784 1612 2112 784
Size: 828 500 3231
Fragments arranged by size:
3231 828 500
StyI C'CwwG_G
Cuts at: 107 1693 2047 107
Size: 1586 354 2619
Fragments arranged by size:
2619 1586 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2142 2275 2734 2943 2142
Size: 133 459 209 3758
Fragments arranged by size:
3758 459 209 133
TatI w'GTAC_w
Cuts at: 617 656 1020 4074 617
Size: 39 364 3054 1102
Fragments arranged by size:
3054 1102 364 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 499 531 4076 4108 499
Size: 32 3545 32 950
Fragments arranged by size:
3545 950 32 32
Tth111I GACn'n_nGTC
Cuts at: 2356 2356
Size: 4559
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2054
Size: 4559
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
XmnI GAAnn'nnTTC
Cuts at: 986 1190 986
Size: 204 4355
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsaXI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI