(Circular) (Six-base) MAPSORT of: pF1KB7742.seq Check: 9672 from: 1 to: 4571 pF1KB7742 4571 bp With 182 enzymes: SgfI * June 26, 2008 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 876 876 Size: 4571 AccI GT'mk_AC Cuts at: 1609 1609 Size: 4571 Acc65I G'GTAC_C Cuts at: 1587 1587 Size: 4571 AclI AA'CG_TT Cuts at: 4411 4411 Size: 4571 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 841 1337 2396 2828 3575 3843 841 Size: 496 1059 432 747 268 1569 Fragments arranged by size: 1569 1059 747 496 432 268 AfeI AGC'GCT Cuts at: 732 732 Size: 4571 AflIII A'CryG_T Cuts at: 1923 3027 4004 1923 Size: 1104 977 2490 Fragments arranged by size: 2490 1104 977 AgeI A'CCGG_T Cuts at: 2959 2959 Size: 4571 AlwNI CAG_nnn'CTG Cuts at: 181 1048 1198 1876 3443 181 Size: 867 150 678 1567 1309 Fragments arranged by size: 1567 1309 867 678 150 ApaI G_GGCC'C Cuts at: 338 338 Size: 4571 ApaLI G'TGCA_C Cuts at: 3341 3341 Size: 4571 ApoI r'AATT_y Cuts at: 829 1575 3924 4180 829 Size: 746 2349 256 1220 Fragments arranged by size: 2349 1220 746 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4571 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4571 AvaI C'yCGr_G Cuts at: 1210 1222 1309 1591 1210 Size: 12 87 282 4190 Fragments arranged by size: 4190 282 87 12 AvrII C'CTAG_G Cuts at: 1144 1144 Size: 4571 BamHI G'GATC_C Cuts at: 1596 1596 Size: 4571 BanI G'GyrC_C Cuts at: 808 1587 2001 2249 2284 808 Size: 779 414 248 35 3095 Fragments arranged by size: 3095 779 414 248 35 BanII G_rGCy'C Cuts at: 338 934 1130 1211 1223 1585 2615 338 Size: 596 196 81 12 362 1030 2294 Fragments arranged by size: 2294 1030 596 362 196 81 12 BbeI G_GCGC'C Cuts at: 2005 2253 2005 Size: 248 4323 BbsI GAAGACnn'nnnn_ Cuts at: 784 784 Size: 4571 BbvCI CC'TCA_GC Cuts at: 277 845 277 Size: 568 4003 BciVI GTATCCnnnnn_n' Cuts at: 2469 3230 4571 2469 Size: 761 1341 2469 Fragments arranged by size: 2469 1341 761 BclI T'GATC_A Cuts at: 2091 2091 Size: 4571 BglI GCCn_nnn'nGGC Cuts at: 1098 1098 Size: 4571 BglII A'GATC_T Cuts at: 2086 4123 2086 Size: 2037 2534 BlpI GC'TnA_GC Cuts at: 1683 1683 Size: 4571 Bme1580I G_kGCm'C Cuts at: 338 813 1085 2196 2289 3345 338 Size: 475 272 1111 93 1056 1564 Fragments arranged by size: 1564 1111 1056 475 272 93 BmrI ACTGGGnnnn_n' Cuts at: 1072 1783 1864 2199 4343 1072 Size: 711 81 335 2144 1300 Fragments arranged by size: 2144 1300 711 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 453 2938 453 Size: 2485 2086 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1004 1147 1582 1004 Size: 143 435 3993 Fragments arranged by size: 3993 435 143 Bpu10I CC'TnA_GC Cuts at: 277 845 1199 1205 1217 277 Size: 568 354 6 12 3631 Fragments arranged by size: 3631 568 354 12 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1747 2626 3118 3416 1747 Size: 879 492 298 2902 Fragments arranged by size: 2902 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 145 374 4275 145 Size: 229 3901 441 Fragments arranged by size: 3901 441 229 BsaAI yAC'GTr Cuts at: 1924 1924 Size: 4571 BsaBI GATnn'nnATC Cuts at: 6 2085 4122 6 Size: 2079 2037 455 Fragments arranged by size: 2079 2037 455 BsaHI Gr'CG_yC Cuts at: 784 2002 2250 2952 4448 784 Size: 1218 248 702 1496 907 Fragments arranged by size: 1496 1218 907 702 248 BsaWI w'CCGG_w Cuts at: 1458 1760 2281 2959 3233 3380 3791 1458 Size: 302 521 678 274 147 411 2238 Fragments arranged by size: 2238 678 521 411 302 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 310 862 886 942 993 1022 1138 1267 1315 Size: 552 24 56 51 29 116 129 48 Cuts at: 1315 310 Size: 3566 Fragments arranged by size: 3566 552 129 116 56 51 48 29 24 BseYI C'CCAG_C Cuts at: 1131 1366 1372 1378 1996 2754 3331 3827 1131 Size: 235 6 6 618 758 577 496 1875 Fragments arranged by size: 1875 758 618 577 496 235 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 419 419 Size: 4571 BsiEI CG_ry'CG Cuts at: 104 2113 2159 3367 104 Size: 2009 46 1208 1308 Fragments arranged by size: 2009 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1310 1585 2363 2553 3345 1310 Size: 275 778 190 792 2536 Fragments arranged by size: 2536 792 778 275 190 BsmBI CGTCTCn'nnnn_ Cuts at: 640 640 Size: 4571 Bsp1286I G_dGCh'C Cuts at: 338 813 934 1085 1130 1211 1223 1310 1585 Size: 475 121 151 45 81 12 87 275 Cuts at: 1585 2196 2289 2363 2553 2615 3345 338 Size: 611 93 74 190 62 730 1564 Fragments arranged by size: 1564 730 611 475 275 190 151 121 93 87 81 74 62 45 12 BspEI T'CCGG_A Cuts at: 1458 3791 1458 Size: 2333 2238 BspHI T'CATG_A Cuts at: 3747 3747 Size: 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 876 1621 1835 2137 2518 876 Size: 745 214 302 381 2929 Fragments arranged by size: 2929 745 381 302 214 BsrBI CCG'CTC Cuts at: 50 2863 2917 4401 4566 50 Size: 2813 54 1484 165 55 Fragments arranged by size: 2813 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2483 2483 Size: 4571 BsrFI r'CCGG_y Cuts at: 584 978 2569 2750 2959 584 Size: 394 1591 181 209 2196 Fragments arranged by size: 2196 1591 394 209 181 BssSI C'ACGA_G Cuts at: 569 658 2842 3200 569 Size: 89 2184 358 1940 Fragments arranged by size: 2184 1940 358 89 BstAPI GCAn_nnn'nTGC Cuts at: 1948 1948 Size: 4571 BstBI TT'CG_AA Cuts at: 2932 2932 Size: 4571 BstXI CCAn_nnnn'nTGG Cuts at: 355 355 Size: 4571 BstYI r'GATC_y Cuts at: 1 177 1397 1596 2063 2086 2421 2667 3679 Size: 176 1220 199 467 23 335 246 1012 Cuts at: 3679 3765 3777 4123 1 Size: 86 12 346 449 Fragments arranged by size: 1220 1012 467 449 346 335 246 199 176 86 23 12 Bsu36I CC'TnA_GG Cuts at: 935 935 Size: 4571 BtgI C'CryG_G Cuts at: 146 348 813 839 1518 146 Size: 202 465 26 679 3199 Fragments arranged by size: 3199 679 465 202 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2521 2702 2865 2521 Size: 181 163 4227 Fragments arranged by size: 4227 181 163 BtsI GCAGTG_nn' Cuts at: 407 985 1814 407 Size: 578 829 3164 Fragments arranged by size: 3164 829 578 DraI TTT'AAA Cuts at: 1570 1570 Size: 4571 DraIII CAC_nnn'GTG Cuts at: 1080 1080 Size: 4571 DrdI GACnn_nn'nnGTC Cuts at: 2277 3135 2277 Size: 858 3713 EaeI y'GGCC_r Cuts at: 582 693 890 1521 2156 2330 2721 2748 4093 Size: 111 197 631 635 174 391 27 1345 Cuts at: 4093 582 Size: 1060 Fragments arranged by size: 1345 1060 635 631 391 197 174 111 27 EagI C'GGCC_G Cuts at: 2156 2156 Size: 4571 EarI CTCTTCn'nnn_ Cuts at: 444 847 863 959 1169 1540 2594 2804 444 Size: 403 16 96 210 371 1054 210 2211 Fragments arranged by size: 2211 1054 403 371 210 210 96 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2779 3089 3235 4381 2779 Size: 310 146 1146 2969 Fragments arranged by size: 2969 1146 310 146 EcoICRI GAG'CTC Cuts at: 1583 1583 Size: 4571 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 841 1004 1147 1337 1582 2396 2828 3575 3843 Size: 163 143 190 245 814 432 747 268 Cuts at: 3843 841 Size: 1569 Fragments arranged by size: 1569 814 747 432 268 245 190 163 143 EcoO109I rG'GnC_Cy Cuts at: 334 756 1710 334 Size: 422 954 3195 Fragments arranged by size: 3195 954 422 EcoRI G'AATT_C Cuts at: 1575 1575 Size: 4571 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2590 2590 Size: 4571 FspI TGC'GCA Cuts at: 2352 2352 Size: 4571 HaeII r_GCGC'y Cuts at: 734 2005 2253 3275 4254 734 Size: 1271 248 1022 979 1051 Fragments arranged by size: 1271 1051 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 313 345 2413 2443 2445 2475 313 Size: 32 2068 30 2 30 2409 Fragments arranged by size: 2409 2068 32 30 30 2 HincII GTy'rAC Cuts at: 1610 1610 Size: 4571 HindIII A'AGCT_T Cuts at: 1555 1555 Size: 4571 KasI G'GCGC_C Cuts at: 2001 2249 2001 Size: 248 4323 KpnI G_GTAC'C Cuts at: 1591 1591 Size: 4571 MluI A'CGCG_T Cuts at: 4004 4004 Size: 4571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3242 3426 3242 Size: 184 4387 MscI TGG'CCA Cuts at: 695 892 1523 2332 695 Size: 197 631 809 2934 Fragments arranged by size: 2934 809 631 197 MslI CAynn'nnrTG Cuts at: 353 413 1483 2560 2687 353 Size: 60 1070 1077 127 2237 Fragments arranged by size: 2237 1077 1070 127 60 MspA1I CmG'CkG Cuts at: 276 1405 1682 1996 2356 3369 3614 276 Size: 1129 277 314 360 1013 245 1233 Fragments arranged by size: 1233 1129 1013 360 314 277 245 NaeI GCC'GGC Cuts at: 2752 2752 Size: 4571 NarI GG'CG_CC Cuts at: 2002 2250 2002 Size: 248 4323 NcoI C'CATG_G Cuts at: 348 839 1518 348 Size: 491 679 3401 Fragments arranged by size: 3401 679 491 NgoMIV G'CCGG_C Cuts at: 2750 2750 Size: 4571 NspI r_CATG'y Cuts at: 1624 2124 3031 1624 Size: 500 907 3164 Fragments arranged by size: 3164 907 500 PasI CC'CwG_GG Cuts at: 156 156 Size: 4571 PciI A'CATG_T Cuts at: 3027 3027 Size: 4571 PfoI T'CCnGG_A Cuts at: 1109 1109 Size: 4571 PmeI GTTT'AAAC Cuts at: 1570 1570 Size: 4571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 3710 Size: 32 4539 PsiI TTA'TAA Cuts at: 527 773 527 Size: 246 4325 PspOMI G'GGCC_C Cuts at: 334 334 Size: 4571 PspXI vC'TCGA_Gb Cuts at: 1309 1309 Size: 4571 PstI C_TGCA'G Cuts at: 1618 2303 1618 Size: 685 3886 PvuI CG_AT'CG Cuts at: 104 104 Size: 4571 PvuII CAG'CTG Cuts at: 276 1405 1996 2356 276 Size: 1129 591 360 2491 Fragments arranged by size: 2491 1129 591 360 RsrII CG'GwC_CG Cuts at: 2766 2766 Size: 4571 SacI G_AGCT'C Cuts at: 1585 1585 Size: 4571 SalI G'TCGA_C Cuts at: 1608 1608 Size: 4571 SapI GCTCTTCn'nnn_ Cuts at: 959 1169 2594 2804 959 Size: 210 1425 210 2726 Fragments arranged by size: 2726 1425 210 210 SbfI CC_TGCA'GG Cuts at: 1618 1618 Size: 4571 ScaI AGT'ACT Cuts at: 4088 4088 Size: 4571 SfcI C'TryA_G Cuts at: 33 119 1614 2299 3292 3483 33 Size: 86 1495 685 993 191 1121 Fragments arranged by size: 1495 1121 993 685 191 86 SfoI GGC'GCC Cuts at: 2003 2251 2003 Size: 248 4323 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4571 SmaI CCC'GGG Cuts at: 1593 1593 Size: 4571 SmlI C'TyrA_G Cuts at: 1309 1726 2641 3133 3395 1309 Size: 417 915 492 262 2485 Fragments arranged by size: 2485 915 492 417 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4571 SphI G_CATG'C Cuts at: 1624 2124 1624 Size: 500 4071 StuI AGG'CCT Cuts at: 1012 1160 1012 Size: 148 4423 StyI C'CwwG_G Cuts at: 348 680 839 1144 1425 1518 1705 2059 348 Size: 332 159 305 281 93 187 354 2860 Fragments arranged by size: 2860 354 332 305 281 187 159 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 398 731 1091 2154 2287 2746 2955 398 Size: 333 360 1063 133 459 209 2014 Fragments arranged by size: 2014 1063 459 360 333 209 133 TatI w'GTAC_w Cuts at: 4086 4086 Size: 4571 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4088 4120 4088 Size: 32 4539 Tth111I GACn'n_nGTC Cuts at: 2368 2368 Size: 4571 XbaI T'CTAG_A Cuts at: 1602 1602 Size: 4571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1525 2066 1525 Size: 541 4030 XhoI C'TCGA_G Cuts at: 1309 1309 Size: 4571 XmaI C'CCGG_G Cuts at: 1591 1591 Size: 4571 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AflII AhdI AleI AlfI AloI AscI BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsrGI BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI ZraI