Restriction map for
pF1KB7742
(Circular) (Six-base) MAPSORT of: pF1KB7742.seq Check: 9672 from: 1 to: 4571
pF1KB7742 4571 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 876 876
Size: 4571
AccI GT'mk_AC
Cuts at: 1609 1609
Size: 4571
Acc65I G'GTAC_C
Cuts at: 1587 1587
Size: 4571
AclI AA'CG_TT
Cuts at: 4411 4411
Size: 4571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1337 2396 2828 3575 3843 841
Size: 496 1059 432 747 268 1569
Fragments arranged by size:
1569 1059 747 496 432 268
AfeI AGC'GCT
Cuts at: 732 732
Size: 4571
AflIII A'CryG_T
Cuts at: 1923 3027 4004 1923
Size: 1104 977 2490
Fragments arranged by size:
2490 1104 977
AgeI A'CCGG_T
Cuts at: 2959 2959
Size: 4571
AlwNI CAG_nnn'CTG
Cuts at: 181 1048 1198 1876 3443 181
Size: 867 150 678 1567 1309
Fragments arranged by size:
1567 1309 867 678 150
ApaI G_GGCC'C
Cuts at: 338 338
Size: 4571
ApaLI G'TGCA_C
Cuts at: 3341 3341
Size: 4571
ApoI r'AATT_y
Cuts at: 829 1575 3924 4180 829
Size: 746 2349 256 1220
Fragments arranged by size:
2349 1220 746 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4571
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
AvaI C'yCGr_G
Cuts at: 1210 1222 1309 1591 1210
Size: 12 87 282 4190
Fragments arranged by size:
4190 282 87 12
AvrII C'CTAG_G
Cuts at: 1144 1144
Size: 4571
BamHI G'GATC_C
Cuts at: 1596 1596
Size: 4571
BanI G'GyrC_C
Cuts at: 808 1587 2001 2249 2284 808
Size: 779 414 248 35 3095
Fragments arranged by size:
3095 779 414 248 35
BanII G_rGCy'C
Cuts at: 338 934 1130 1211 1223 1585 2615 338
Size: 596 196 81 12 362 1030 2294
Fragments arranged by size:
2294 1030 596 362 196 81 12
BbeI G_GCGC'C
Cuts at: 2005 2253 2005
Size: 248 4323
BbsI GAAGACnn'nnnn_
Cuts at: 784 784
Size: 4571
BbvCI CC'TCA_GC
Cuts at: 277 845 277
Size: 568 4003
BciVI GTATCCnnnnn_n'
Cuts at: 2469 3230 4571 2469
Size: 761 1341 2469
Fragments arranged by size:
2469 1341 761
BclI T'GATC_A
Cuts at: 2091 2091
Size: 4571
BglI GCCn_nnn'nGGC
Cuts at: 1098 1098
Size: 4571
BglII A'GATC_T
Cuts at: 2086 4123 2086
Size: 2037 2534
BlpI GC'TnA_GC
Cuts at: 1683 1683
Size: 4571
Bme1580I G_kGCm'C
Cuts at: 338 813 1085 2196 2289 3345 338
Size: 475 272 1111 93 1056 1564
Fragments arranged by size:
1564 1111 1056 475 272 93
BmrI ACTGGGnnnn_n'
Cuts at: 1072 1783 1864 2199 4343 1072
Size: 711 81 335 2144 1300
Fragments arranged by size:
2144 1300 711 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 453 2938 453
Size: 2485 2086
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1147 1582 1004
Size: 143 435 3993
Fragments arranged by size:
3993 435 143
Bpu10I CC'TnA_GC
Cuts at: 277 845 1199 1205 1217 277
Size: 568 354 6 12 3631
Fragments arranged by size:
3631 568 354 12 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1747 2626 3118 3416 1747
Size: 879 492 298 2902
Fragments arranged by size:
2902 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 145 374 4275 145
Size: 229 3901 441
Fragments arranged by size:
3901 441 229
BsaAI yAC'GTr
Cuts at: 1924 1924
Size: 4571
BsaBI GATnn'nnATC
Cuts at: 6 2085 4122 6
Size: 2079 2037 455
Fragments arranged by size:
2079 2037 455
BsaHI Gr'CG_yC
Cuts at: 784 2002 2250 2952 4448 784
Size: 1218 248 702 1496 907
Fragments arranged by size:
1496 1218 907 702 248
BsaWI w'CCGG_w
Cuts at: 1458 1760 2281 2959 3233 3380 3791 1458
Size: 302 521 678 274 147 411 2238
Fragments arranged by size:
2238 678 521 411 302 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 310 862 886 942 993 1022 1138 1267 1315
Size: 552 24 56 51 29 116 129 48
Cuts at: 1315 310
Size: 3566
Fragments arranged by size:
3566 552 129 116 56 51 48 29
24
BseYI C'CCAG_C
Cuts at: 1131 1366 1372 1378 1996 2754 3331 3827 1131
Size: 235 6 6 618 758 577 496 1875
Fragments arranged by size:
1875 758 618 577 496 235 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 419
Size: 4571
BsiEI CG_ry'CG
Cuts at: 104 2113 2159 3367 104
Size: 2009 46 1208 1308
Fragments arranged by size:
2009 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1310 1585 2363 2553 3345 1310
Size: 275 778 190 792 2536
Fragments arranged by size:
2536 792 778 275 190
BsmBI CGTCTCn'nnnn_
Cuts at: 640 640
Size: 4571
Bsp1286I G_dGCh'C
Cuts at: 338 813 934 1085 1130 1211 1223 1310 1585
Size: 475 121 151 45 81 12 87 275
Cuts at: 1585 2196 2289 2363 2553 2615 3345 338
Size: 611 93 74 190 62 730 1564
Fragments arranged by size:
1564 730 611 475 275 190 151 121
93 87 81 74 62 45 12
BspEI T'CCGG_A
Cuts at: 1458 3791 1458
Size: 2333 2238
BspHI T'CATG_A
Cuts at: 3747 3747
Size: 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 876 1621 1835 2137 2518 876
Size: 745 214 302 381 2929
Fragments arranged by size:
2929 745 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2863 2917 4401 4566 50
Size: 2813 54 1484 165 55
Fragments arranged by size:
2813 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2483 2483
Size: 4571
BsrFI r'CCGG_y
Cuts at: 584 978 2569 2750 2959 584
Size: 394 1591 181 209 2196
Fragments arranged by size:
2196 1591 394 209 181
BssSI C'ACGA_G
Cuts at: 569 658 2842 3200 569
Size: 89 2184 358 1940
Fragments arranged by size:
2184 1940 358 89
BstAPI GCAn_nnn'nTGC
Cuts at: 1948 1948
Size: 4571
BstBI TT'CG_AA
Cuts at: 2932 2932
Size: 4571
BstXI CCAn_nnnn'nTGG
Cuts at: 355 355
Size: 4571
BstYI r'GATC_y
Cuts at: 1 177 1397 1596 2063 2086 2421 2667 3679
Size: 176 1220 199 467 23 335 246 1012
Cuts at: 3679 3765 3777 4123 1
Size: 86 12 346 449
Fragments arranged by size:
1220 1012 467 449 346 335 246 199
176 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 935 935
Size: 4571
BtgI C'CryG_G
Cuts at: 146 348 813 839 1518 146
Size: 202 465 26 679 3199
Fragments arranged by size:
3199 679 465 202 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2521 2702 2865 2521
Size: 181 163 4227
Fragments arranged by size:
4227 181 163
BtsI GCAGTG_nn'
Cuts at: 407 985 1814 407
Size: 578 829 3164
Fragments arranged by size:
3164 829 578
DraI TTT'AAA
Cuts at: 1570 1570
Size: 4571
DraIII CAC_nnn'GTG
Cuts at: 1080 1080
Size: 4571
DrdI GACnn_nn'nnGTC
Cuts at: 2277 3135 2277
Size: 858 3713
EaeI y'GGCC_r
Cuts at: 582 693 890 1521 2156 2330 2721 2748 4093
Size: 111 197 631 635 174 391 27 1345
Cuts at: 4093 582
Size: 1060
Fragments arranged by size:
1345 1060 635 631 391 197 174 111
27
EagI C'GGCC_G
Cuts at: 2156 2156
Size: 4571
EarI CTCTTCn'nnn_
Cuts at: 444 847 863 959 1169 1540 2594 2804 444
Size: 403 16 96 210 371 1054 210 2211
Fragments arranged by size:
2211 1054 403 371 210 210 96 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2779 3089 3235 4381 2779
Size: 310 146 1146 2969
Fragments arranged by size:
2969 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1583 1583
Size: 4571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1004 1147 1337 1582 2396 2828 3575 3843
Size: 163 143 190 245 814 432 747 268
Cuts at: 3843 841
Size: 1569
Fragments arranged by size:
1569 814 747 432 268 245 190 163
143
EcoO109I rG'GnC_Cy
Cuts at: 334 756 1710 334
Size: 422 954 3195
Fragments arranged by size:
3195 954 422
EcoRI G'AATT_C
Cuts at: 1575 1575
Size: 4571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4571
FspI TGC'GCA
Cuts at: 2352 2352
Size: 4571
HaeII r_GCGC'y
Cuts at: 734 2005 2253 3275 4254 734
Size: 1271 248 1022 979 1051
Fragments arranged by size:
1271 1051 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 2413 2443 2445 2475 313
Size: 32 2068 30 2 30 2409
Fragments arranged by size:
2409 2068 32 30 30 2
HincII GTy'rAC
Cuts at: 1610 1610
Size: 4571
HindIII A'AGCT_T
Cuts at: 1555 1555
Size: 4571
KasI G'GCGC_C
Cuts at: 2001 2249 2001
Size: 248 4323
KpnI G_GTAC'C
Cuts at: 1591 1591
Size: 4571
MluI A'CGCG_T
Cuts at: 4004 4004
Size: 4571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3242 3426 3242
Size: 184 4387
MscI TGG'CCA
Cuts at: 695 892 1523 2332 695
Size: 197 631 809 2934
Fragments arranged by size:
2934 809 631 197
MslI CAynn'nnrTG
Cuts at: 353 413 1483 2560 2687 353
Size: 60 1070 1077 127 2237
Fragments arranged by size:
2237 1077 1070 127 60
MspA1I CmG'CkG
Cuts at: 276 1405 1682 1996 2356 3369 3614 276
Size: 1129 277 314 360 1013 245 1233
Fragments arranged by size:
1233 1129 1013 360 314 277 245
NaeI GCC'GGC
Cuts at: 2752 2752
Size: 4571
NarI GG'CG_CC
Cuts at: 2002 2250 2002
Size: 248 4323
NcoI C'CATG_G
Cuts at: 348 839 1518 348
Size: 491 679 3401
Fragments arranged by size:
3401 679 491
NgoMIV G'CCGG_C
Cuts at: 2750 2750
Size: 4571
NspI r_CATG'y
Cuts at: 1624 2124 3031 1624
Size: 500 907 3164
Fragments arranged by size:
3164 907 500
PasI CC'CwG_GG
Cuts at: 156 156
Size: 4571
PciI A'CATG_T
Cuts at: 3027 3027
Size: 4571
PfoI T'CCnGG_A
Cuts at: 1109 1109
Size: 4571
PmeI GTTT'AAAC
Cuts at: 1570 1570
Size: 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4539
PsiI TTA'TAA
Cuts at: 527 773 527
Size: 246 4325
PspOMI G'GGCC_C
Cuts at: 334 334
Size: 4571
PspXI vC'TCGA_Gb
Cuts at: 1309 1309
Size: 4571
PstI C_TGCA'G
Cuts at: 1618 2303 1618
Size: 685 3886
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4571
PvuII CAG'CTG
Cuts at: 276 1405 1996 2356 276
Size: 1129 591 360 2491
Fragments arranged by size:
2491 1129 591 360
RsrII CG'GwC_CG
Cuts at: 2766 2766
Size: 4571
SacI G_AGCT'C
Cuts at: 1585 1585
Size: 4571
SalI G'TCGA_C
Cuts at: 1608 1608
Size: 4571
SapI GCTCTTCn'nnn_
Cuts at: 959 1169 2594 2804 959
Size: 210 1425 210 2726
Fragments arranged by size:
2726 1425 210 210
SbfI CC_TGCA'GG
Cuts at: 1618 1618
Size: 4571
ScaI AGT'ACT
Cuts at: 4088 4088
Size: 4571
SfcI C'TryA_G
Cuts at: 33 119 1614 2299 3292 3483 33
Size: 86 1495 685 993 191 1121
Fragments arranged by size:
1495 1121 993 685 191 86
SfoI GGC'GCC
Cuts at: 2003 2251 2003
Size: 248 4323
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
SmaI CCC'GGG
Cuts at: 1593 1593
Size: 4571
SmlI C'TyrA_G
Cuts at: 1309 1726 2641 3133 3395 1309
Size: 417 915 492 262 2485
Fragments arranged by size:
2485 915 492 417 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4571
SphI G_CATG'C
Cuts at: 1624 2124 1624
Size: 500 4071
StuI AGG'CCT
Cuts at: 1012 1160 1012
Size: 148 4423
StyI C'CwwG_G
Cuts at: 348 680 839 1144 1425 1518 1705 2059 348
Size: 332 159 305 281 93 187 354 2860
Fragments arranged by size:
2860 354 332 305 281 187 159 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 398 731 1091 2154 2287 2746 2955 398
Size: 333 360 1063 133 459 209 2014
Fragments arranged by size:
2014 1063 459 360 333 209 133
TatI w'GTAC_w
Cuts at: 4086 4086
Size: 4571
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4088 4120 4088
Size: 32 4539
Tth111I GACn'n_nGTC
Cuts at: 2368 2368
Size: 4571
XbaI T'CTAG_A
Cuts at: 1602 1602
Size: 4571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1525 2066 1525
Size: 541 4030
XhoI C'TCGA_G
Cuts at: 1309 1309
Size: 4571
XmaI C'CCGG_G
Cuts at: 1591 1591
Size: 4571
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PpuMI PshAI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI