Restriction map for
pF1KB7754
(Circular) (Six-base) MAPSORT of: pF1KB7754.seq Check: 5804 from: 1 to: 4601
pF1KB7754 4601 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 1355 1355
Size: 4601
AccI GT'mk_AC
Cuts at: 1639 1639
Size: 4601
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 572 802 1492 1521 2426 2858 3605 3873
Size: 341 230 690 29 905 432 747 268
Cuts at: 3873 231
Size: 959
Fragments arranged by size:
959 905 747 690 432 341 268 230
29
AfeI AGC'GCT
Cuts at: 1282 1282
Size: 4601
AflIII A'CryG_T
Cuts at: 587 1184 1204 1343 1345 1953 3057 4034 587
Size: 597 20 139 2 608 1104 977 1154
Fragments arranged by size:
1154 1104 977 608 597 139 20 2
AgeI A'CCGG_T
Cuts at: 2989 2989
Size: 4601
AhdI GACnn_n'nnGTC
Cuts at: 744 744
Size: 4601
AleI CACnn'nnGTG
Cuts at: 1060 1060
Size: 4601
AlwNI CAG_nnn'CTG
Cuts at: 291 357 663 1120 1906 3473 291
Size: 66 306 457 786 1567 1419
Fragments arranged by size:
1567 1419 786 457 306 66
ApaLI G'TGCA_C
Cuts at: 3371 3371
Size: 4601
ApoI r'AATT_y
Cuts at: 1098 1605 3954 4210 1098
Size: 507 2349 256 1489
Fragments arranged by size:
2349 1489 507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 1080 1621 1080
Size: 541 4060
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 1617 2031 2279 2314 1617
Size: 414 248 35 3904
Fragments arranged by size:
3904 414 248 35
BanII G_rGCy'C
Cuts at: 407 1129 1615 2645 407
Size: 722 486 1030 2363
Fragments arranged by size:
2363 1030 722 486
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 483 483
Size: 4601
BbvCI CC'TCA_GC
Cuts at: 325 827 325
Size: 502 4099
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3260 4601 2499
Size: 761 1341 2499
Fragments arranged by size:
2499 1341 761
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BglII A'GATC_T
Cuts at: 849 2116 4153 849
Size: 1267 2037 1297
Fragments arranged by size:
2037 1297 1267
BlpI GC'TnA_GC
Cuts at: 1713 1713
Size: 4601
Bme1580I G_kGCm'C
Cuts at: 2226 2319 3375 2226
Size: 93 1056 3452
Fragments arranged by size:
3452 1056 93
BmgBI CAC'GTC
Cuts at: 219 588 1185 219
Size: 369 597 3635
Fragments arranged by size:
3635 597 369
BmrI ACTGGGnnnn_n'
Cuts at: 1123 1813 1894 2229 4373 1123
Size: 690 81 335 2144 1351
Fragments arranged by size:
2144 1351 690 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 472 546 391
Size: 81 74 4446
Fragments arranged by size:
4446 81 74
Bpu10I CC'TnA_GC
Cuts at: 325 827 325
Size: 502 4099
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1028 1777 2656 3148 3446 1028
Size: 749 879 492 298 2183
Fragments arranged by size:
2183 879 749 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 747 4305 747
Size: 3558 1043
BsaAI yAC'GTr
Cuts at: 240 1205 1346 1954 240
Size: 965 141 608 2887
Fragments arranged by size:
2887 965 608 141
BsaBI GATnn'nnATC
Cuts at: 6 2115 4152 6
Size: 2109 2037 455
Fragments arranged by size:
2109 2037 455
BsaHI Gr'CG_yC
Cuts at: 1352 1560 2032 2280 2982 4478 1352
Size: 208 472 248 702 1496 1475
Fragments arranged by size:
1496 1475 702 472 248 208
BsaWI w'CCGG_w
Cuts at: 1495 1790 2311 2989 3263 3410 3821 1495
Size: 295 521 678 274 147 411 2275
Fragments arranged by size:
2275 678 521 411 295 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 597 986 1011 597
Size: 389 25 4187
Fragments arranged by size:
4187 389 25
BseYI C'CCAG_C
Cuts at: 1090 2026 2784 3361 3857 1090
Size: 936 758 577 496 1834
Fragments arranged by size:
1834 936 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 547 454
Size: 93 4508
BsiEI CG_ry'CG
Cuts at: 104 2143 2189 3397 104
Size: 2039 46 1208 1308
Fragments arranged by size:
2039 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 407 1481 1615 2393 2583 3375 407
Size: 1074 134 778 190 792 1633
Fragments arranged by size:
1633 1074 792 778 190 134
BsmBI CGTCTCn'nnnn_
Cuts at: 968 968
Size: 4601
Bsp1286I G_dGCh'C
Cuts at: 407 1129 1481 1615 2226 2319 2393 2583 2645
Size: 722 352 134 611 93 74 190 62
Cuts at: 2645 3375 407
Size: 730 1633
Fragments arranged by size:
1633 730 722 611 352 190 134 93
74 62
BspEI T'CCGG_A
Cuts at: 1495 3821 1495
Size: 2326 2275
BspHI T'CATG_A
Cuts at: 383 419 3777 383
Size: 36 3358 1207
Fragments arranged by size:
3358 1207 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 467 1651 1865 2167 2548 467
Size: 1184 214 302 381 2520
Fragments arranged by size:
2520 1184 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2893 2947 4431 4596 50
Size: 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 2599 2780 2989 2599
Size: 181 209 4211
Fragments arranged by size:
4211 209 181
BssSI C'ACGA_G
Cuts at: 2872 3230 2872
Size: 358 4243
BstAPI GCAn_nnn'nTGC
Cuts at: 663 1978 663
Size: 1315 3286
BstBI TT'CG_AA
Cuts at: 1427 2962 1427
Size: 1535 3066
BstEII G'GTnAC_C
Cuts at: 498 736 498
Size: 238 4363
BstXI CCAn_nnnn'nTGG
Cuts at: 852 1062 852
Size: 210 4391
BstYI r'GATC_y
Cuts at: 1 353 659 777 849 1626 2093 2116 2451
Size: 352 306 118 72 777 467 23 335
Cuts at: 2451 2697 3709 3795 3807 4153 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 777 467 449 352 346 335 306
246 118 86 72 23 12
BtgI C'CryG_G
Cuts at: 107 175 507 107
Size: 68 332 4201
Fragments arranged by size:
4201 332 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2551 2732 2895 2551
Size: 181 163 4257
Fragments arranged by size:
4257 181 163
BtsI GCAGTG_nn'
Cuts at: 1162 1844 1162
Size: 682 3919
DraI TTT'AAA
Cuts at: 1600 1600
Size: 4601
DraIII CAC_nnn'GTG
Cuts at: 219 219
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3165 2307
Size: 858 3743
EaeI y'GGCC_r
Cuts at: 1146 2186 2360 2751 2778 4123 1146
Size: 1040 174 391 27 1345 1624
Fragments arranged by size:
1624 1345 1040 391 174 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 791 1031 1189 2624 2834 791
Size: 240 158 1435 210 2558
Fragments arranged by size:
2558 1435 240 210 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2809 3119 3265 4411 2809
Size: 310 146 1146 2999
Fragments arranged by size:
2999 1146 310 146
EcoICRI GAG'CTC
Cuts at: 405 1613 405
Size: 1208 3393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 391 472 546 572 802 1492 1521 2426
Size: 160 81 74 26 230 690 29 905
Cuts at: 2426 2858 3605 3873 231
Size: 432 747 268 959
Fragments arranged by size:
959 905 747 690 432 268 230 160
81 74 29 26
EcoO109I rG'GnC_Cy
Cuts at: 456 672 876 1117 1337 1740 456
Size: 216 204 241 220 403 3317
Fragments arranged by size:
3317 403 241 220 216 204
EcoRI G'AATT_C
Cuts at: 1098 1605 1098
Size: 507 4094
EcoRV GAT'ATC
Cuts at: 540 540
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 783 1401 2620 783
Size: 618 1219 2764
Fragments arranged by size:
2764 1219 618
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 1284 2035 2283 3305 4284 1284
Size: 751 248 1022 979 1601
Fragments arranged by size:
1601 1022 979 751 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 730 762 1006 1038 2443 2473 2475 2505 730
Size: 32 244 32 1405 30 2 30 2826
Fragments arranged by size:
2826 1405 244 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1640 1640
Size: 4601
HindIII A'AGCT_T
Cuts at: 947 947
Size: 4601
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 146 254 3272 3456 146
Size: 108 3018 184 1291
Fragments arranged by size:
3018 1291 184 108
MscI TGG'CCA
Cuts at: 1148 2362 1148
Size: 1214 3387
MslI CAynn'nnrTG
Cuts at: 129 367 1060 1142 2590 2717 129
Size: 238 693 82 1448 127 2013
Fragments arranged by size:
2013 1448 693 238 127 82
MspA1I CmG'CkG
Cuts at: 260 962 1712 2026 2386 3399 3644 260
Size: 702 750 314 360 1013 245 1217
Fragments arranged by size:
1217 1013 750 702 360 314 245
NaeI GCC'GGC
Cuts at: 2782 2782
Size: 4601
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NcoI C'CATG_G
Cuts at: 107 107
Size: 4601
NgoMIV G'CCGG_C
Cuts at: 2780 2780
Size: 4601
NspI r_CATG'y
Cuts at: 366 1181 1654 2154 3061 366
Size: 815 473 500 907 1906
Fragments arranged by size:
1906 907 815 500 473
PasI CC'CwG_GG
Cuts at: 668 668
Size: 4601
PciI A'CATG_T
Cuts at: 3057 3057
Size: 4601
PflMI CCAn_nnn'nTGG
Cuts at: 488 488
Size: 4601
PfoI T'CCnGG_A
Cuts at: 356 685 356
Size: 329 4272
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PmlI CAC'GTG
Cuts at: 240 1346 240
Size: 1106 3495
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 456 672 876 456
Size: 216 204 4181
Fragments arranged by size:
4181 216 204
PstI C_TGCA'G
Cuts at: 659 1648 2333 659
Size: 989 685 2927
Fragments arranged by size:
2927 989 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 260 2026 2386 260
Size: 1766 360 2475
Fragments arranged by size:
2475 1766 360
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 407 1615 407
Size: 1208 3393
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SanDI GG'GwC_CC
Cuts at: 672 672
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 1031 2624 2834 1031
Size: 1593 210 2798
Fragments arranged by size:
2798 1593 210
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 4118 4118
Size: 4601
SfcI C'TryA_G
Cuts at: 33 655 1458 1644 2329 3322 3513 33
Size: 622 803 186 685 993 191 1121
Fragments arranged by size:
1121 993 803 685 622 191 186
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1082 1623 1082
Size: 541 4060
SmlI C'TyrA_G
Cuts at: 1043 1756 2671 3163 3425 1043
Size: 713 915 492 262 2219
Fragments arranged by size:
2219 915 713 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
StyI C'CwwG_G
Cuts at: 107 767 1157 1735 2089 107
Size: 660 390 578 354 2619
Fragments arranged by size:
2619 660 578 390 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1387 2184 2317 2776 2985 1387
Size: 797 133 459 209 3003
Fragments arranged by size:
3003 797 459 209 133
TatI w'GTAC_w
Cuts at: 4116 4116
Size: 4601
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 1632 1632
Size: 4601
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 765 2096 765
Size: 1331 3270
XmaI C'CCGG_G
Cuts at: 1080 1621 1080
Size: 541 4060
XmnI GAAnn'nnTTC
Cuts at: 1282 1366 1423 1450 1282
Size: 84 57 27 4433
Fragments arranged by size:
4433 84 57 27
ZraI GAC'GTC
Cuts at: 1353 1353
Size: 4601
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AlfI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmtI BsaXI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI