Restriction map for
pF1KB7756
(Circular) (Six-base) MAPSORT of: pF1KB7756.seq Check: 9601 from: 1 to: 4607
pF1KB7756 4607 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 277 1645 277
Size: 1368 3239
Acc65I G'GTAC_C
Cuts at: 1623 1623
Size: 4607
AclI AA'CG_TT
Cuts at: 4447 4447
Size: 4607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 210 500 696 780 864 948 1116 1200 1284
Size: 290 196 84 84 84 168 84 84
Cuts at: 1284 1368 2432 2864 3611 3879 210
Size: 84 1064 432 747 268 938
Fragments arranged by size:
1064 938 747 432 290 268 196 168
84 84 84 84 84 84
AflIII A'CryG_T
Cuts at: 1959 3063 4040 1959
Size: 1104 977 2526
Fragments arranged by size:
2526 1104 977
AgeI A'CCGG_T
Cuts at: 2995 2995
Size: 4607
AleI CACnn'nnGTG
Cuts at: 1513 1513
Size: 4607
AlwNI CAG_nnn'CTG
Cuts at: 142 719 971 1034 1139 1912 3479 142
Size: 577 252 63 105 773 1567 1270
Fragments arranged by size:
1567 1270 773 577 252 105 63
ApaI G_GGCC'C
Cuts at: 209 209
Size: 4607
ApaLI G'TGCA_C
Cuts at: 3377 3377
Size: 4607
ApoI r'AATT_y
Cuts at: 998 1378 1611 3960 4216 998
Size: 380 233 2349 256 1389
Fragments arranged by size:
2349 1389 380 256 233
AseI AT'TA_AT
Cuts at: 20 20
Size: 4607
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
AvaI C'yCGr_G
Cuts at: 209 1627 209
Size: 1418 3189
BamHI G'GATC_C
Cuts at: 214 1632 214
Size: 1418 3189
BanI G'GyrC_C
Cuts at: 266 1623 2037 2285 2320 266
Size: 1357 414 248 35 2553
Fragments arranged by size:
2553 1357 414 248 35
BanII G_rGCy'C
Cuts at: 209 808 1396 1480 1621 2651 209
Size: 599 588 84 141 1030 2165
Fragments arranged by size:
2165 1030 599 588 141 84
BbeI G_GCGC'C
Cuts at: 2041 2289 2041
Size: 248 4359
BbsI GAAGACnn'nnnn_
Cuts at: 419 419
Size: 4607
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4573
BciVI GTATCCnnnnn_n'
Cuts at: 299 2505 3266 4607 299
Size: 2206 761 1341 299
Fragments arranged by size:
2206 1341 761 299
BclI T'GATC_A
Cuts at: 2127 2127
Size: 4607
BfrBI ATG'CAT
Cuts at: 688 688
Size: 4607
BglII A'GATC_T
Cuts at: 2122 4159 2122
Size: 2037 2570
BlpI GC'TnA_GC
Cuts at: 975 1395 1719 975
Size: 420 324 3863
Fragments arranged by size:
3863 420 324
Bme1580I G_kGCm'C
Cuts at: 209 599 2232 2325 3381 209
Size: 390 1633 93 1056 1435
Fragments arranged by size:
1633 1435 1056 390 93
BmrI ACTGGGnnnn_n'
Cuts at: 366 1268 1819 1900 2235 4379 366
Size: 902 551 81 335 2144 594
Fragments arranged by size:
2144 902 594 551 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 827 2974 827
Size: 2147 2460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 416 424 776 860 133
Size: 283 8 352 84 3880
Fragments arranged by size:
3880 352 283 84 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1783 2662 3154 3452 568
Size: 1215 879 492 298 1723
Fragments arranged by size:
1723 1215 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 196 561 603 4311 196
Size: 365 42 3708 492
Fragments arranged by size:
3708 492 365 42
BsaAI yAC'GTr
Cuts at: 1960 1960
Size: 4607
BsaBI GATnn'nnATC
Cuts at: 6 2121 4158 6
Size: 2115 2037 455
Fragments arranged by size:
2115 2037 455
BsaHI Gr'CG_yC
Cuts at: 2038 2286 2988 4484 2038
Size: 248 702 1496 2161
Fragments arranged by size:
2161 1496 702 248
BsaWI w'CCGG_w
Cuts at: 922 1006 1426 1553 1796 2317 2995 3269 3416
Size: 84 420 127 243 521 678 274 147
Cuts at: 3416 3827 922
Size: 411 1702
Fragments arranged by size:
1702 678 521 420 411 274 243 147
127 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 4607
BseYI C'CCAG_C
Cuts at: 202 353 2032 2790 3367 3863 202
Size: 151 1679 758 577 496 946
Fragments arranged by size:
1679 946 758 577 496 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 795 879 1131 1299 1578 795
Size: 84 252 168 279 3824
Fragments arranged by size:
3824 279 252 168 84
BsiEI CG_ry'CG
Cuts at: 104 2149 2195 3403 104
Size: 2045 46 1208 1308
Fragments arranged by size:
2045 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 808 1396 1480 1621 2399 2589 3381 808
Size: 588 84 141 778 190 792 2034
Fragments arranged by size:
2034 792 778 588 190 141 84
BsmI GAATG_Cn'
Cuts at: 894 1030 1534 894
Size: 136 504 3967
Fragments arranged by size:
3967 504 136
Bsp1286I G_dGCh'C
Cuts at: 209 599 808 1396 1480 1621 2232 2325 2399
Size: 390 209 588 84 141 611 93 74
Cuts at: 2399 2589 2651 3381 209
Size: 190 62 730 1435
Fragments arranged by size:
1435 730 611 588 390 209 190 141
93 84 74 62
BspEI T'CCGG_A
Cuts at: 3827 3827
Size: 4607
BspHI T'CATG_A
Cuts at: 3783 3783
Size: 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 425 1657 1871 2173 2554 425
Size: 1232 214 302 381 2478
Fragments arranged by size:
2478 1232 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2899 2953 4437 4602 50
Size: 2849 54 1484 165 55
Fragments arranged by size:
2849 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2519 2519
Size: 4607
BsrFI r'CCGG_y
Cuts at: 2605 2786 2995 2605
Size: 181 209 4217
Fragments arranged by size:
4217 209 181
BssSI C'ACGA_G
Cuts at: 271 2878 3236 271
Size: 2607 358 1642
Fragments arranged by size:
2607 1642 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1984 1984
Size: 4607
BstBI TT'CG_AA
Cuts at: 2968 2968
Size: 4607
BstYI r'GATC_y
Cuts at: 1 214 1632 2099 2122 2457 2703 3715 3801
Size: 213 1418 467 23 335 246 1012 86
Cuts at: 3801 3813 4159 1
Size: 12 346 449
Fragments arranged by size:
1418 1012 467 449 346 335 246 213
86 23 12
BtgI C'CryG_G
Cuts at: 283 531 599 283
Size: 248 68 4291
Fragments arranged by size:
4291 248 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2557 2738 2901 2557
Size: 181 163 4263
Fragments arranged by size:
4263 181 163
BtsI GCAGTG_nn'
Cuts at: 237 783 867 1850 237
Size: 546 84 983 2994
Fragments arranged by size:
2994 983 546 84
DraI TTT'AAA
Cuts at: 1606 1606
Size: 4607
DrdI GACnn_nn'nnGTC
Cuts at: 2313 3171 2313
Size: 858 3749
EaeI y'GGCC_r
Cuts at: 2192 2366 2757 2784 4129 2192
Size: 174 391 27 1345 2670
Fragments arranged by size:
2670 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2192 2192
Size: 4607
EarI CTCTTCn'nnn_
Cuts at: 547 739 867 2630 2840 547
Size: 192 128 1763 210 2314
Fragments arranged by size:
2314 1763 210 192 128
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2815 3125 3271 4417 2815
Size: 310 146 1146 3005
Fragments arranged by size:
3005 1146 310 146
EcoICRI GAG'CTC
Cuts at: 806 1394 1478 1619 806
Size: 588 84 141 3794
Fragments arranged by size:
3794 588 141 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 210 416 424 500 696 776 780 860
Size: 77 206 8 76 196 80 4 80
Cuts at: 860 864 948 1116 1200 1284 1368 2432 2864
Size: 4 84 168 84 84 84 1064 432
Cuts at: 2864 3611 3879 133
Size: 747 268 861
Fragments arranged by size:
1064 861 747 432 268 206 196 168
84 84 84 84 80 80 77 76
8 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 134 134
Size: 4607
EcoO109I rG'GnC_Cy
Cuts at: 139 206 1746 139
Size: 67 1540 3000
Fragments arranged by size:
3000 1540 67
EcoRI G'AATT_C
Cuts at: 998 1611 998
Size: 613 3994
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4607
FspI TGC'GCA
Cuts at: 1143 2388 1143
Size: 1245 3362
HaeII r_GCGC'y
Cuts at: 2041 2289 3311 4290 2041
Size: 248 1022 979 2358
Fragments arranged by size:
2358 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 560 578 592 1299 1331 2449 2479 2481
Size: 14 18 14 707 32 1118 30 2
Cuts at: 2481 2511 546
Size: 30 2642
Fragments arranged by size:
2642 1118 707 32 30 30 18 14
14 2
HincII GTy'rAC
Cuts at: 1646 1646
Size: 4607
HindIII A'AGCT_T
Cuts at: 482 482
Size: 4607
KasI G'GCGC_C
Cuts at: 2037 2285 2037
Size: 248 4359
KpnI G_GTAC'C
Cuts at: 1627 1627
Size: 4607
MluI A'CGCG_T
Cuts at: 4040 4040
Size: 4607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 3278 3462 356
Size: 2922 184 1501
Fragments arranged by size:
2922 1501 184
MscI TGG'CCA
Cuts at: 2368 2368
Size: 4607
MslI CAynn'nnrTG
Cuts at: 687 1359 1513 2596 2723 687
Size: 672 154 1083 127 2571
Fragments arranged by size:
2571 1083 672 154 127
MspA1I CmG'CkG
Cuts at: 1718 2032 2392 3405 3650 1718
Size: 314 360 1013 245 2675
Fragments arranged by size:
2675 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 4607
NarI GG'CG_CC
Cuts at: 2038 2286 2038
Size: 248 4359
NdeI CA'TA_TG
Cuts at: 684 684
Size: 4607
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 4607
NsiI A_TGCA'T
Cuts at: 690 690
Size: 4607
NspI r_CATG'y
Cuts at: 692 1660 2160 3067 692
Size: 968 500 907 2232
Fragments arranged by size:
2232 968 907 500
PasI CC'CwG_GG
Cuts at: 517 517
Size: 4607
PciI A'CATG_T
Cuts at: 3063 3063
Size: 4607
PfoI T'CCnGG_A
Cuts at: 319 319
Size: 4607
PmeI GTTT'AAAC
Cuts at: 1606 1606
Size: 4607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4575
PspOMI G'GGCC_C
Cuts at: 205 205
Size: 4607
PstI C_TGCA'G
Cuts at: 1654 2339 1654
Size: 685 3922
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4607
PvuII CAG'CTG
Cuts at: 2032 2392 2032
Size: 360 4247
RsrII CG'GwC_CG
Cuts at: 2802 2802
Size: 4607
SacI G_AGCT'C
Cuts at: 808 1396 1480 1621 808
Size: 588 84 141 3794
Fragments arranged by size:
3794 588 141 84
SalI G'TCGA_C
Cuts at: 1644 1644
Size: 4607
SapI GCTCTTCn'nnn_
Cuts at: 547 739 867 2630 2840 547
Size: 192 128 1763 210 2314
Fragments arranged by size:
2314 1763 210 192 128
SbfI CC_TGCA'GG
Cuts at: 1654 1654
Size: 4607
ScaI AGT'ACT
Cuts at: 125 4124 125
Size: 3999 608
SfcI C'TryA_G
Cuts at: 33 936 1188 1650 2335 3328 3519 33
Size: 903 252 462 685 993 191 1121
Fragments arranged by size:
1121 993 903 685 462 252 191
SfoI GGC'GCC
Cuts at: 2039 2287 2039
Size: 248 4359
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
SmaI CCC'GGG
Cuts at: 211 1629 211
Size: 1418 3189
SmlI C'TyrA_G
Cuts at: 547 1762 2677 3169 3431 547
Size: 1215 915 492 262 1723
Fragments arranged by size:
1723 1215 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4607
SphI G_CATG'C
Cuts at: 1660 2160 1660
Size: 500 4107
StuI AGG'CCT
Cuts at: 793 1045 1129 1213 1297 1549 793
Size: 252 84 84 84 252 3851
Fragments arranged by size:
3851 252 252 84 84 84
StyI C'CwwG_G
Cuts at: 1741 2095 1741
Size: 354 4253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2190 2323 2782 2991 2190
Size: 133 459 209 3806
Fragments arranged by size:
3806 459 209 133
TatI w'GTAC_w
Cuts at: 123 4122 123
Size: 3999 608
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4124 4156 4124
Size: 32 4575
Tth111I GACn'n_nGTC
Cuts at: 2404 2404
Size: 4607
XbaI T'CTAG_A
Cuts at: 1638 1638
Size: 4607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2102
Size: 4607
XmaI C'CCGG_G
Cuts at: 209 1627 209
Size: 1418 3189
XmnI GAAnn'nnTTC
Cuts at: 556 793 961 1045 1129 1213 1297 1381 1549
Size: 237 168 84 84 84 84 84 168
Cuts at: 1549 556
Size: 3614
Fragments arranged by size:
3614 237 168 168 84 84 84 84
84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NheI NotI NruI PacI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI