Restriction map for
pF1KB7757
(Circular) (Six-base) MAPSORT of: pF1KB7757.seq Check: 232 from: 1 to: 4454
pF1KB7757 4454 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1492 1492
Size: 4454
Acc65I G'GTAC_C
Cuts at: 1470 1470
Size: 4454
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2711 3458 3726 2279
Size: 432 747 268 3007
Fragments arranged by size:
3007 747 432 268
AflIII A'CryG_T
Cuts at: 566 617 1806 2910 3887 566
Size: 51 1189 1104 977 1133
Fragments arranged by size:
1189 1133 1104 977 51
AgeI A'CCGG_T
Cuts at: 2842 2842
Size: 4454
AleI CACnn'nnGTG
Cuts at: 890 1333 890
Size: 443 4011
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 692 692
Size: 4454
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 394 426 394
Size: 32 4422
AlwNI CAG_nnn'CTG
Cuts at: 1759 3326 1759
Size: 1567 2887
ApaI G_GGCC'C
Cuts at: 499 704 499
Size: 205 4249
ApaLI G'TGCA_C
Cuts at: 991 1171 3224 991
Size: 180 2053 2221
Fragments arranged by size:
2221 2053 180
ApoI r'AATT_y
Cuts at: 1059 1458 3807 4063 1059
Size: 399 2349 256 1450
Fragments arranged by size:
2349 1450 399 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 354 538 609 802 1474 354
Size: 184 71 193 672 3334
Fragments arranged by size:
3334 672 193 184 71
BamHI G'GATC_C
Cuts at: 1479 1479
Size: 4454
BanI G'GyrC_C
Cuts at: 241 309 733 739 1470 1884 2132 2167 241
Size: 68 424 6 731 414 248 35 2528
Fragments arranged by size:
2528 731 424 414 248 68 35 6
BanII G_rGCy'C
Cuts at: 499 704 782 803 1279 1468 2498 499
Size: 205 78 21 476 189 1030 2455
Fragments arranged by size:
2455 1030 476 205 189 78 21
BbeI G_GCGC'C
Cuts at: 245 313 737 743 1888 2136 245
Size: 68 424 6 1145 248 2563
Fragments arranged by size:
2563 1145 424 248 68 6
BbsI GAAGACnn'nnnn_
Cuts at: 1035 1035
Size: 4454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 538 572 777 811 1026 1060 90
Size: 34 414 34 205 34 215 34 3484
Fragments arranged by size:
3484 414 215 205 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3113 4454 2352
Size: 761 1341 2352
Fragments arranged by size:
2352 1341 761
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4454
BglI GCCn_nnn'nGGC
Cuts at: 365 503 365
Size: 138 4316
BglII A'GATC_T
Cuts at: 1969 4006 1969
Size: 2037 2417
BlpI GC'TnA_GC
Cuts at: 1566 1566
Size: 4454
Bme1580I G_kGCm'C
Cuts at: 499 704 995 1175 2079 2172 3228 499
Size: 205 291 180 904 93 1056 1725
Fragments arranged by size:
1725 1056 904 291 205 180 93
BmgBI CAC'GTC
Cuts at: 189 204 302 363 873 189
Size: 15 98 61 510 3770
Fragments arranged by size:
3770 510 98 61 15
BmrI ACTGGGnnnn_n'
Cuts at: 123 254 1223 1666 1747 2082 4226 123
Size: 131 969 443 81 335 2144 351
Fragments arranged by size:
2144 969 443 351 335 131 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1630 2509 3001 3299 1630
Size: 879 492 298 2785
Fragments arranged by size:
2785 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4158 4158
Size: 4454
BsaAI yAC'GTr
Cuts at: 888 1170 1807 888
Size: 282 637 3535
Fragments arranged by size:
3535 637 282
BsaBI GATnn'nnATC
Cuts at: 6 1968 4005 6
Size: 1962 2037 455
Fragments arranged by size:
2037 1962 455
BsaHI Gr'CG_yC
Cuts at: 119 242 310 515 734 740 1885 2133 2835
Size: 123 68 205 219 6 1145 248 702
Cuts at: 2835 4331 119
Size: 1496 242
Fragments arranged by size:
1496 1145 702 248 242 219 205 123
68 6
BsaWI w'CCGG_w
Cuts at: 630 1643 2164 2842 3116 3263 3674 630
Size: 1013 521 678 274 147 411 1410
Fragments arranged by size:
1410 1013 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 352 382 1249 1279 1300 1330 352
Size: 30 867 30 21 30 3476
Fragments arranged by size:
3476 867 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 520 943 1257 1371 1374 520
Size: 423 314 114 3 3600
Fragments arranged by size:
3600 423 314 114 3
BseYI C'CCAG_C
Cuts at: 135 1879 2637 3214 3710 135
Size: 1744 758 577 496 879
Fragments arranged by size:
1744 879 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 673
Size: 4454
BsiEI CG_ry'CG
Cuts at: 104 143 284 482 631 1152 1295 1996 2042
Size: 39 141 198 149 521 143 701 46
Cuts at: 2042 3250 104
Size: 1208 1308
Fragments arranged by size:
1308 1208 701 521 198 149 143 141
46 39
BsiHKAI G_wGCw'C
Cuts at: 671 782 887 995 1175 1468 2246 2436 3228
Size: 111 105 108 180 293 778 190 792
Cuts at: 3228 671
Size: 1897
Fragments arranged by size:
1897 792 778 293 190 180 111 108
105
BsmBI CGTCTCn'nnnn_
Cuts at: 194 194
Size: 4454
Bsp1286I G_dGCh'C
Cuts at: 499 671 704 782 803 887 995 1175 1279
Size: 172 33 78 21 84 108 180 104
Cuts at: 1279 1468 2079 2172 2246 2436 2498 3228 499
Size: 189 611 93 74 190 62 730 1725
Fragments arranged by size:
1725 730 611 190 189 180 172 108
104 93 84 78 74 62 33 21
BspEI T'CCGG_A
Cuts at: 3674 3674
Size: 4454
BspHI T'CATG_A
Cuts at: 3630 3630
Size: 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1504 1718 2020 2401 1504
Size: 214 302 381 3557
Fragments arranged by size:
3557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2746 2800 4284 4449 50
Size: 2696 54 1484 165 55
Fragments arranged by size:
2696 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2366 2366
Size: 4454
BsrFI r'CCGG_y
Cuts at: 121 283 559 736 1127 1290 1405 2452 2633
Size: 162 276 177 391 163 115 1047 181
Cuts at: 2633 2842 121
Size: 209 1733
Fragments arranged by size:
1733 1047 391 276 209 181 177 163
162 115
BssHII G'CGCG_C
Cuts at: 1048 1048
Size: 4454
BssSI C'ACGA_G
Cuts at: 268 547 856 1176 2725 3083 268
Size: 279 309 320 1549 358 1639
Fragments arranged by size:
1639 1549 358 320 309 279
BstAPI GCAn_nnn'nTGC
Cuts at: 1205 1831 1205
Size: 626 3828
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstEII G'GTnAC_C
Cuts at: 648 648
Size: 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4454
BstYI r'GATC_y
Cuts at: 1 1067 1479 1946 1969 2304 2550 3562 3648
Size: 1066 412 467 23 335 246 1012 86
Cuts at: 3648 3660 4006 1
Size: 12 346 449
Fragments arranged by size:
1066 1012 467 449 412 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 179 518 878 179
Size: 339 360 3755
Fragments arranged by size:
3755 360 339
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2404 2585 2748 2404
Size: 181 163 4110
Fragments arranged by size:
4110 181 163
BtsI GCAGTG_nn'
Cuts at: 1697 1697
Size: 4454
DraI TTT'AAA
Cuts at: 1064 1453 1064
Size: 389 4065
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3018 2160
Size: 858 3596
EaeI y'GGCC_r
Cuts at: 191 281 285 479 504 561 642 719 812
Size: 90 4 194 25 57 81 77 93
Cuts at: 812 1292 1407 2039 2213 2604 2631 3976 191
Size: 480 115 632 174 391 27 1345 669
Fragments arranged by size:
1345 669 632 480 391 194 174 115
93 90 81 77 57 27 25 4
EagI C'GGCC_G
Cuts at: 281 479 1292 2039 281
Size: 198 813 747 2696
Fragments arranged by size:
2696 813 747 198
EarI CTCTTCn'nnn_
Cuts at: 538 1428 2477 2687 538
Size: 890 1049 210 2305
Fragments arranged by size:
2305 1049 890 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2662 2972 3118 4264 2662
Size: 310 146 1146 2852
Fragments arranged by size:
2852 1146 310 146
EcoICRI GAG'CTC
Cuts at: 780 1466 780
Size: 686 3768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2711 3458 3726 2279
Size: 432 747 268 3007
Fragments arranged by size:
3007 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 495 1593 495
Size: 1098 3356
EcoRI G'AATT_C
Cuts at: 1458 1458
Size: 4454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4454
FseI GG_CCGG'CC
Cuts at: 287 287
Size: 4454
FspI TGC'GCA
Cuts at: 1240 2235 1240
Size: 995 3459
HaeII r_GCGC'y
Cuts at: 245 313 737 743 1134 1888 2136 3158 4137
Size: 68 424 6 391 754 248 1022 979
Cuts at: 4137 245
Size: 562
Fragments arranged by size:
1022 979 754 562 424 391 248 68
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2296 2326 2328 2358 2296
Size: 30 2 30 4392
Fragments arranged by size:
4392 30 30 2
HincII GTy'rAC
Cuts at: 585 978 1493 585
Size: 393 515 3546
Fragments arranged by size:
3546 515 393
HpaI GTT'AAC
Cuts at: 978 978
Size: 4454
KasI G'GCGC_C
Cuts at: 241 309 733 739 1884 2132 241
Size: 68 424 6 1145 248 2563
Fragments arranged by size:
2563 1145 424 248 68 6
KpnI G_GTAC'C
Cuts at: 1474 1474
Size: 4454
MluI A'CGCG_T
Cuts at: 566 3887 566
Size: 3321 1133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3125 3309 3125
Size: 184 4270
MscI TGG'CCA
Cuts at: 814 2215 814
Size: 1401 3053
MslI CAynn'nnrTG
Cuts at: 709 890 988 1168 1333 2443 2570 709
Size: 181 98 180 165 1110 127 2593
Fragments arranged by size:
2593 1110 181 180 165 127 98
MspA1I CmG'CkG
Cuts at: 181 520 687 810 1565 1879 2239 3252 3497
Size: 339 167 123 755 314 360 1013 245
Cuts at: 3497 181
Size: 1138
Fragments arranged by size:
1138 1013 755 360 339 314 245 167
123
NaeI GCC'GGC
Cuts at: 123 285 561 738 1292 1407 2635 123
Size: 162 276 177 554 115 1228 1942
Fragments arranged by size:
1942 1228 554 276 177 162 115
NarI GG'CG_CC
Cuts at: 242 310 734 740 1885 2133 242
Size: 68 424 6 1145 248 2563
Fragments arranged by size:
2563 1145 424 248 68 6
NgoMIV G'CCGG_C
Cuts at: 121 283 559 736 1290 1405 2633 121
Size: 162 276 177 554 115 1228 1942
Fragments arranged by size:
1942 1228 554 276 177 162 115
NspI r_CATG'y
Cuts at: 621 759 1167 1507 2007 2914 621
Size: 138 408 340 500 907 2161
Fragments arranged by size:
2161 907 500 408 340 138
PasI CC'CwG_GG
Cuts at: 336 336
Size: 4454
PciI A'CATG_T
Cuts at: 617 2910 617
Size: 2293 2161
PfoI T'CCnGG_A
Cuts at: 113 113
Size: 4454
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PmlI CAC'GTG
Cuts at: 1170 1170
Size: 4454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4422
PspOMI G'GGCC_C
Cuts at: 495 700 495
Size: 205 4249
PstI C_TGCA'G
Cuts at: 1501 2186 1501
Size: 685 3769
PvuI CG_AT'CG
Cuts at: 104 143 104
Size: 39 4415
PvuII CAG'CTG
Cuts at: 687 810 1879 2239 687
Size: 123 1069 360 2902
Fragments arranged by size:
2902 1069 360 123
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4454
SacI G_AGCT'C
Cuts at: 782 1468 782
Size: 686 3768
SacII CC_GC'GG
Cuts at: 182 521 182
Size: 339 4115
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SfcI C'TryA_G
Cuts at: 33 1497 2182 3175 3366 33
Size: 1464 685 993 191 1121
Fragments arranged by size:
1464 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 503 503
Size: 4454
SfoI GGC'GCC
Cuts at: 243 311 735 741 1886 2134 243
Size: 68 424 6 1145 248 2563
Fragments arranged by size:
2563 1145 424 248 68 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SgrAI Cr'CCGG_yG
Cuts at: 736 736
Size: 4454
SmaI CCC'GGG
Cuts at: 356 540 1476 356
Size: 184 936 3334
Fragments arranged by size:
3334 936 184
SmlI C'TyrA_G
Cuts at: 1609 2524 3016 3278 1609
Size: 915 492 262 2785
Fragments arranged by size:
2785 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 1507 2007 1507
Size: 500 3954
StyI C'CwwG_G
Cuts at: 1588 1942 1588
Size: 354 4100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2037 2170 2629 2838 2037
Size: 133 459 209 3653
Fragments arranged by size:
3653 459 209 133
TatI w'GTAC_w
Cuts at: 3969 3969
Size: 4454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 352 384 1249 1281 3971 4003 352
Size: 32 865 32 2690 32 803
Fragments arranged by size:
2690 865 803 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2251 2251
Size: 4454
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 969 1949 969
Size: 980 3474
XmaI C'CCGG_G
Cuts at: 354 538 1474 354
Size: 184 936 3334
Fragments arranged by size:
3334 936 184
XmnI GAAnn'nnTTC
Cuts at: 1099 1099
Size: 4454
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BbvCI BfrBI BmtI BpmI Bpu10I BsiWI BsmI BsrGI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FspAI
HindIII MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PflMI PpuMI PshAI PsiI PspXI PsrI SanDI
SexAI SnaBI SrfI SspI StuI SwaI XhoI ZraI