Restriction map for
pF1KB7769
(Circular) (Six-base) MAPSORT of: pF1KB7769.seq Check: 605 from: 1 to: 4463
pF1KB7769 4463 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1065 1065
Size: 4463
AccI GT'mk_AC
Cuts at: 1501 1501
Size: 4463
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1302 2288 2720 3467 3735 487
Size: 815 986 432 747 268 1215
Fragments arranged by size:
1215 986 815 747 432 268
AflIII A'CryG_T
Cuts at: 1103 1815 2919 3896 1103
Size: 712 1104 977 1670
Fragments arranged by size:
1670 1104 977 712
AgeI A'CCGG_T
Cuts at: 755 2851 755
Size: 2096 2367
AhdI GACnn_n'nnGTC
Cuts at: 1137 1410 1137
Size: 273 4190
AleI CACnn'nnGTG
Cuts at: 1172 1172
Size: 4463
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 916 916
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 270 303 330 1388 1768 3335 270
Size: 33 27 1058 380 1567 1398
Fragments arranged by size:
1567 1398 1058 380 33 27
ApaI G_GGCC'C
Cuts at: 964 964
Size: 4463
ApaLI G'TGCA_C
Cuts at: 3233 3233
Size: 4463
ApoI r'AATT_y
Cuts at: 1467 3816 4072 1467
Size: 2349 256 1858
Fragments arranged by size:
2349 1858 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 227 346 906 1194 1483 227
Size: 119 560 288 289 3207
Fragments arranged by size:
3207 560 289 288 119
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 639 680 1479 1893 2141 2176 639
Size: 41 799 414 248 35 2926
Fragments arranged by size:
2926 799 414 248 41 35
BanII G_rGCy'C
Cuts at: 520 617 964 1477 2507 520
Size: 97 347 513 1030 2476
Fragments arranged by size:
2476 1030 513 347 97
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BbsI GAAGACnn'nnnn_
Cuts at: 502 502
Size: 4463
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 185 219 90
Size: 34 61 34 4334
Fragments arranged by size:
4334 61 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3122 4463 2361
Size: 761 1341 2361
Fragments arranged by size:
2361 1341 761
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BglI GCCn_nnn'nGGC
Cuts at: 1361 1361
Size: 4463
BglII A'GATC_T
Cuts at: 1978 4015 1978
Size: 2037 2426
BlpI GC'TnA_GC
Cuts at: 592 1575 592
Size: 983 3480
Bme1580I G_kGCm'C
Cuts at: 337 683 964 1043 2088 2181 3237 337
Size: 346 281 79 1045 93 1056 1563
Fragments arranged by size:
1563 1056 1045 346 281 93 79
BmrI ACTGGGnnnn_n'
Cuts at: 786 846 1675 1756 2091 4235 786
Size: 60 829 81 335 2144 1014
Fragments arranged by size:
2144 1014 829 335 81 60
BmtI G_CTAG'C
Cuts at: 1204 1204
Size: 4463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 303 2830 303
Size: 2527 1936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 340 630 811 1093 325
Size: 15 290 181 282 3695
Fragments arranged by size:
3695 290 282 181 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 1639 2518 3010 3308 184
Size: 1455 879 492 298 1339
Fragments arranged by size:
1455 1339 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 148 816 4167 148
Size: 668 3351 444
Fragments arranged by size:
3351 668 444
BsaAI yAC'GTr
Cuts at: 1106 1816 1106
Size: 710 3753
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 887 1371 1894 2142 2844 4340 887
Size: 484 523 248 702 1496 1010
Fragments arranged by size:
1496 1010 702 523 484 248
BsaWI w'CCGG_w
Cuts at: 755 1160 1652 2173 2851 3125 3272 3683 755
Size: 405 492 521 678 274 147 411 1535
Fragments arranged by size:
1535 678 521 492 411 405 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 327 357 973 1003 1294 1324 327
Size: 30 616 30 291 30 3466
Fragments arranged by size:
3466 616 291 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 406 625 751 1315 181
Size: 225 219 126 564 3329
Fragments arranged by size:
3329 564 225 219 126
BseYI C'CCAG_C
Cuts at: 861 947 1888 2646 3223 3719 861
Size: 86 941 758 577 496 1605
Fragments arranged by size:
1605 941 758 577 496 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 1332 1207
Size: 125 4338
BsiEI CG_ry'CG
Cuts at: 104 1447 2005 2051 3259 104
Size: 1343 558 46 1208 1308
Fragments arranged by size:
1343 1308 1208 558 46
BsiHKAI G_wGCw'C
Cuts at: 482 1477 2255 2445 3237 482
Size: 995 778 190 792 1708
Fragments arranged by size:
1708 995 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1057 1057
Size: 4463
Bsp1286I G_dGCh'C
Cuts at: 337 482 520 617 683 964 1043 1477 2088
Size: 145 38 97 66 281 79 434 611
Cuts at: 2088 2181 2255 2445 2507 3237 337
Size: 93 74 190 62 730 1563
Fragments arranged by size:
1563 730 611 434 281 190 145 97
93 79 74 66 62 38
BspEI T'CCGG_A
Cuts at: 3683 3683
Size: 4463
BspHI T'CATG_A
Cuts at: 3639 3639
Size: 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 211 1065 1513 1727 2029 2410 211
Size: 854 448 214 302 381 2264
Fragments arranged by size:
2264 854 448 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2755 2809 4293 4458 50
Size: 2705 54 1484 165 55
Fragments arranged by size:
2705 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2375 2375
Size: 4463
BsrFI r'CCGG_y
Cuts at: 755 969 1077 1442 2461 2642 2851 755
Size: 214 108 365 1019 181 209 2367
Fragments arranged by size:
2367 1019 365 214 209 181 108
BssSI C'ACGA_G
Cuts at: 1170 2734 3092 1170
Size: 1564 358 2541
Fragments arranged by size:
2541 1564 358
BstAPI GCAn_nnn'nTGC
Cuts at: 477 1840 477
Size: 1363 3100
BstBI TT'CG_AA
Cuts at: 2824 2824
Size: 4463
BstEII G'GTnAC_C
Cuts at: 821 821
Size: 4463
BstYI r'GATC_y
Cuts at: 1 350 1488 1955 1978 2313 2559 3571 3657
Size: 349 1138 467 23 335 246 1012 86
Cuts at: 3657 3669 4015 1
Size: 12 346 449
Fragments arranged by size:
1138 1012 467 449 349 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 278 389 528 674 278
Size: 111 139 146 4067
Fragments arranged by size:
4067 146 139 111
BtgI C'CryG_G
Cuts at: 684 1122 684
Size: 438 4025
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 799 2413 2594 2757 799
Size: 1614 181 163 2505
Fragments arranged by size:
2505 1614 181 163
BtsI GCAGTG_nn'
Cuts at: 337 606 1120 1389 1706 337
Size: 269 514 269 317 3094
Fragments arranged by size:
3094 514 317 269 269
DraI TTT'AAA
Cuts at: 1462 1462
Size: 4463
DrdI GACnn_nn'nnGTC
Cuts at: 540 2169 3027 540
Size: 1629 858 1976
Fragments arranged by size:
1976 1629 858
EaeI y'GGCC_r
Cuts at: 376 1119 1392 1444 2048 2222 2613 2640 3985
Size: 743 273 52 604 174 391 27 1345
Cuts at: 3985 376
Size: 854
Fragments arranged by size:
1345 854 743 604 391 273 174 52
27
EagI C'GGCC_G
Cuts at: 1444 2048 1444
Size: 604 3859
EarI CTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2671 2981 3127 4273 2671
Size: 310 146 1146 2861
Fragments arranged by size:
2861 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 340 487 630 811 1093 1302 2288 2720
Size: 15 147 143 181 282 209 986 432
Cuts at: 2720 3467 3735 325
Size: 747 268 1053
Fragments arranged by size:
1053 986 747 432 282 268 209 181
147 143 15
EcoNI CCTnn'n_nnAGG
Cuts at: 469 551 469
Size: 82 4381
EcoO109I rG'GnC_Cy
Cuts at: 124 223 556 960 1279 1602 124
Size: 99 333 404 319 323 2985
Fragments arranged by size:
2985 404 333 323 319 99
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4463
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 1897 2145 3167 4146 1897
Size: 248 1022 979 2214
Fragments arranged by size:
2214 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 909 941 973 1005 1123 1155 1256 1288 1396
Size: 32 32 32 118 32 101 32 108
Cuts at: 1396 1428 2305 2335 2337 2367 909
Size: 32 877 30 2 30 3005
Fragments arranged by size:
3005 877 118 108 101 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1502 1502
Size: 4463
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3134 3318 3134
Size: 184 4279
MscI TGG'CCA
Cuts at: 2224 2224
Size: 4463
MslI CAynn'nnrTG
Cuts at: 1172 2452 2579 1172
Size: 1280 127 3056
Fragments arranged by size:
3056 1280 127
MspA1I CmG'CkG
Cuts at: 375 400 596 1124 1322 1335 1368 1419 1574
Size: 25 196 528 198 13 33 51 155
Cuts at: 1574 1888 2248 3261 3506 375
Size: 314 360 1013 245 1332
Fragments arranged by size:
1332 1013 528 360 314 245 198 196
155 51 33 25 13
NaeI GCC'GGC
Cuts at: 971 1079 2644 971
Size: 108 1565 2790
Fragments arranged by size:
2790 1565 108
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NcoI C'CATG_G
Cuts at: 684 684
Size: 4463
NgoMIV G'CCGG_C
Cuts at: 969 1077 2642 969
Size: 108 1565 2790
Fragments arranged by size:
2790 1565 108
NheI G'CTAG_C
Cuts at: 1200 1200
Size: 4463
NspI r_CATG'y
Cuts at: 1516 2016 2923 1516
Size: 500 907 3056
Fragments arranged by size:
3056 907 500
PasI CC'CwG_GG
Cuts at: 430 547 816 957 964 1024 430
Size: 117 269 141 7 60 3869
Fragments arranged by size:
3869 269 141 117 60 7
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PflMI CCAn_nnn'nTGG
Cuts at: 216 216
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PmlI CAC'GTG
Cuts at: 1106 1106
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PpuMI rG'GwC_Cy
Cuts at: 124 223 556 124
Size: 99 333 4031
Fragments arranged by size:
4031 333 99
PshAI GACnn'nnGTC
Cuts at: 886 886
Size: 4463
PspOMI G'GGCC_C
Cuts at: 960 960
Size: 4463
PstI C_TGCA'G
Cuts at: 275 422 489 601 895 1062 1384 1510 2195
Size: 147 67 112 294 167 322 126 685
Cuts at: 2195 275
Size: 2543
Fragments arranged by size:
2543 685 322 294 167 147 126 112
67
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 596 1322 1335 1419 1888 2248 596
Size: 726 13 84 469 360 2811
Fragments arranged by size:
2811 726 469 360 84 13
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SacII CC_GC'GG
Cuts at: 1125 1125
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SanDI GG'GwC_CC
Cuts at: 124 124
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 2486 2696 2486
Size: 210 4253
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SfcI C'TryA_G
Cuts at: 33 271 418 485 597 891 1058 1380 1506
Size: 238 147 67 112 294 167 322 126
Cuts at: 1506 2191 3184 3375 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 322 294 238 191 167
147 126 112 67
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 1196 1485 1196
Size: 289 4174
SmlI C'TyrA_G
Cuts at: 163 1618 2533 3025 3287 163
Size: 1455 915 492 262 1339
Fragments arranged by size:
1455 1339 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
StuI AGG'CCT
Cuts at: 358 358
Size: 4463
StyI C'CwwG_G
Cuts at: 290 684 1597 1951 290
Size: 394 913 354 2802
Fragments arranged by size:
2802 913 394 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 351 734 738 2046 2179 2638 2847 351
Size: 383 4 1308 133 459 209 1967
Fragments arranged by size:
1967 1308 459 383 209 133 4
TatI w'GTAC_w
Cuts at: 773 3978 773
Size: 3205 1258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 327 359 620 652 1294 1326 3980 4012 327
Size: 32 261 32 642 32 2654 32 778
Fragments arranged by size:
2654 778 642 261 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1958 1958
Size: 4463
XmaI C'CCGG_G
Cuts at: 1194 1483 1194
Size: 289 4174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BbvCI
BfrBI BmgBI Bpu10I BsiWI BsmI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PfoI
PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI