Restriction map for
pF1KB7771
(Circular) (Six-base) MAPSORT of: pF1KB7771.seq Check: 8820 from: 1 to: 4463
pF1KB7771 4463 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1501 1501
Size: 4463
Acc65I G'GTAC_C
Cuts at: 1479 1479
Size: 4463
AclI AA'CG_TT
Cuts at: 4303 4303
Size: 4463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1049 2288 2720 3467 3735 911
Size: 138 1239 432 747 268 1639
Fragments arranged by size:
1639 1239 747 432 268 138
AflIII A'CryG_T
Cuts at: 294 1815 2919 3896 294
Size: 1521 1104 977 861
Fragments arranged by size:
1521 1104 977 861
AgeI A'CCGG_T
Cuts at: 2851 2851
Size: 4463
AhdI GACnn_n'nnGTC
Cuts at: 1179 1179
Size: 4463
AlwNI CAG_nnn'CTG
Cuts at: 1768 3335 1768
Size: 1567 2896
ApaLI G'TGCA_C
Cuts at: 3233 3233
Size: 4463
ApoI r'AATT_y
Cuts at: 391 739 1467 3816 4072 391
Size: 348 728 2349 256 782
Fragments arranged by size:
2349 782 728 348 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4463
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
AvaI C'yCGr_G
Cuts at: 1483 1483
Size: 4463
BamHI G'GATC_C
Cuts at: 1488 1488
Size: 4463
BanI G'GyrC_C
Cuts at: 1479 1893 2141 2176 1479
Size: 414 248 35 3766
Fragments arranged by size:
3766 414 248 35
BanII G_rGCy'C
Cuts at: 414 1477 2507 414
Size: 1063 1030 2370
Fragments arranged by size:
2370 1063 1030
BbeI G_GCGC'C
Cuts at: 1897 2145 1897
Size: 248 4215
BciVI GTATCCnnnnn_n'
Cuts at: 2361 3122 4463 2361
Size: 761 1341 2361
Fragments arranged by size:
2361 1341 761
BclI T'GATC_A
Cuts at: 1983 1983
Size: 4463
BglII A'GATC_T
Cuts at: 1978 4015 1978
Size: 2037 2426
BlpI GC'TnA_GC
Cuts at: 1575 1575
Size: 4463
Bme1580I G_kGCm'C
Cuts at: 1329 2088 2181 3237 1329
Size: 759 93 1056 2555
Fragments arranged by size:
2555 1056 759 93
BmrI ACTGGGnnnn_n'
Cuts at: 673 1675 1756 2091 4235 673
Size: 1002 81 335 2144 901
Fragments arranged by size:
2144 1002 901 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 4463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 820
Size: 4463
Bpu10I CC'TnA_GC
Cuts at: 588 588
Size: 4463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1639 2518 3010 3308 1639
Size: 879 492 298 2794
Fragments arranged by size:
2794 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 373 4167 373
Size: 3794 669
BsaAI yAC'GTr
Cuts at: 1816 1816
Size: 4463
BsaBI GATnn'nnATC
Cuts at: 6 1977 4014 6
Size: 1971 2037 455
Fragments arranged by size:
2037 1971 455
BsaHI Gr'CG_yC
Cuts at: 1894 2142 2844 4340 1894
Size: 248 702 1496 2017
Fragments arranged by size:
2017 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1652 2173 2851 3125 3272 3683 1652
Size: 521 678 274 147 411 2432
Fragments arranged by size:
2432 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1272 1302 1272
Size: 30 4433
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 888 891 894 897 1412 888
Size: 3 3 3 515 3939
Fragments arranged by size:
3939 515 3 3 3
BseYI C'CCAG_C
Cuts at: 1888 2646 3223 3719 1888
Size: 758 577 496 2632
Fragments arranged by size:
2632 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2005 2051 3259 104
Size: 1901 46 1208 1308
Fragments arranged by size:
1901 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1421 1477 2255 2445 3237 1421
Size: 56 778 190 792 2647
Fragments arranged by size:
2647 792 778 190 56
Bsp1286I G_dGCh'C
Cuts at: 414 1329 1421 1477 2088 2181 2255 2445 2507
Size: 915 92 56 611 93 74 190 62
Cuts at: 2507 3237 414
Size: 730 1640
Fragments arranged by size:
1640 915 730 611 190 93 92 74
62 56
BspEI T'CCGG_A
Cuts at: 3683 3683
Size: 4463
BspHI T'CATG_A
Cuts at: 1062 3639 1062
Size: 2577 1886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1513 1727 2029 2410 1513
Size: 214 302 381 3566
Fragments arranged by size:
3566 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2755 2809 4293 4458 50
Size: 2705 54 1484 165 55
Fragments arranged by size:
2705 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2375 2375
Size: 4463
BsrFI r'CCGG_y
Cuts at: 2461 2642 2851 2461
Size: 181 209 4073
Fragments arranged by size:
4073 209 181
BsrGI T'GTAC_A
Cuts at: 1409 1409
Size: 4463
BssSI C'ACGA_G
Cuts at: 416 1129 2734 3092 416
Size: 713 1605 358 1787
Fragments arranged by size:
1787 1605 713 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1840 1840
Size: 4463
BstBI TT'CG_AA
Cuts at: 477 2824 477
Size: 2347 2116
BstYI r'GATC_y
Cuts at: 1 726 1488 1955 1978 2313 2559 3571 3657
Size: 725 762 467 23 335 246 1012 86
Cuts at: 3657 3669 4015 1
Size: 12 346 449
Fragments arranged by size:
1012 762 725 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1248 1377 107
Size: 1141 129 3193
Fragments arranged by size:
3193 1141 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2413 2594 2757 2413
Size: 181 163 4119
Fragments arranged by size:
4119 181 163
BtsI GCAGTG_nn'
Cuts at: 1706 1706
Size: 4463
ClaI AT'CG_AT
Cuts at: 598 598
Size: 4463
DraI TTT'AAA
Cuts at: 746 1462 746
Size: 716 3747
DrdI GACnn_nn'nnGTC
Cuts at: 2169 3027 2169
Size: 858 3605
EaeI y'GGCC_r
Cuts at: 2048 2222 2613 2640 3985 2048
Size: 174 391 27 1345 2526
Fragments arranged by size:
2526 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2048 2048
Size: 4463
EarI CTCTTCn'nnn_
Cuts at: 841 885 915 1025 1045 1204 1340 2486 2696
Size: 44 30 110 20 159 136 1146 210
Cuts at: 2696 841
Size: 2608
Fragments arranged by size:
2608 1146 210 159 136 110 44 30
20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2671 2981 3127 4273 2671
Size: 310 146 1146 2861
Fragments arranged by size:
2861 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1475 1475
Size: 4463
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 911 1049 2288 2720 3467 3735 820
Size: 91 138 1239 432 747 268 1548
Fragments arranged by size:
1548 1239 747 432 268 138 91
EcoO109I rG'GnC_Cy
Cuts at: 1602 1602
Size: 4463
EcoRI G'AATT_C
Cuts at: 1467 1467
Size: 4463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 162 2482 162
Size: 2320 2143
FspI TGC'GCA
Cuts at: 2244 2244
Size: 4463
HaeII r_GCGC'y
Cuts at: 1897 2145 3167 4146 1897
Size: 248 1022 979 2214
Fragments arranged by size:
2214 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1165 1197 1272 1304 2305 2335 2337 2367 1165
Size: 32 75 32 1001 30 2 30 3261
Fragments arranged by size:
3261 1001 75 32 32 30 30 2
HincII GTy'rAC
Cuts at: 519 1502 519
Size: 983 3480
HindIII A'AGCT_T
Cuts at: 1057 1057
Size: 4463
KasI G'GCGC_C
Cuts at: 1893 2141 1893
Size: 248 4215
KpnI G_GTAC'C
Cuts at: 1483 1483
Size: 4463
MluI A'CGCG_T
Cuts at: 3896 3896
Size: 4463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3134 3318 3134
Size: 184 4279
MscI TGG'CCA
Cuts at: 2224 2224
Size: 4463
MslI CAynn'nnrTG
Cuts at: 2452 2579 2452
Size: 127 4336
MspA1I CmG'CkG
Cuts at: 1574 1888 2248 3261 3506 1574
Size: 314 360 1013 245 2531
Fragments arranged by size:
2531 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2644 2644
Size: 4463
NarI GG'CG_CC
Cuts at: 1894 2142 1894
Size: 248 4215
NcoI C'CATG_G
Cuts at: 107 1377 107
Size: 1270 3193
NdeI CA'TA_TG
Cuts at: 207 264 207
Size: 57 4406
NgoMIV G'CCGG_C
Cuts at: 2642 2642
Size: 4463
NspI r_CATG'y
Cuts at: 1516 2016 2923 1516
Size: 500 907 3056
Fragments arranged by size:
3056 907 500
PciI A'CATG_T
Cuts at: 2919 2919
Size: 4463
PmeI GTTT'AAAC
Cuts at: 1462 1462
Size: 4463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4431
PstI C_TGCA'G
Cuts at: 1510 2195 1510
Size: 685 3778
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4463
PvuII CAG'CTG
Cuts at: 1888 2248 1888
Size: 360 4103
RsrII CG'GwC_CG
Cuts at: 2658 2658
Size: 4463
SacI G_AGCT'C
Cuts at: 1477 1477
Size: 4463
SalI G'TCGA_C
Cuts at: 1500 1500
Size: 4463
SapI GCTCTTCn'nnn_
Cuts at: 841 1340 2486 2696 841
Size: 499 1146 210 2608
Fragments arranged by size:
2608 1146 499 210
SbfI CC_TGCA'GG
Cuts at: 1510 1510
Size: 4463
ScaI AGT'ACT
Cuts at: 3980 3980
Size: 4463
SfcI C'TryA_G
Cuts at: 33 1506 2191 3184 3375 33
Size: 1473 685 993 191 1121
Fragments arranged by size:
1473 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1895 2143 1895
Size: 248 4215
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4463
SmaI CCC'GGG
Cuts at: 1485 1485
Size: 4463
SmlI C'TyrA_G
Cuts at: 1618 2533 3025 3287 1618
Size: 915 492 262 2794
Fragments arranged by size:
2794 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4463
SphI G_CATG'C
Cuts at: 1516 2016 1516
Size: 500 3963
StyI C'CwwG_G
Cuts at: 107 1103 1377 1597 1951 107
Size: 996 274 220 354 2619
Fragments arranged by size:
2619 996 354 274 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 397 1436 2046 2179 2638 2847 397
Size: 1039 610 133 459 209 2013
Fragments arranged by size:
2013 1039 610 459 209 133
TatI w'GTAC_w
Cuts at: 660 1409 3978 660
Size: 749 2569 1145
Fragments arranged by size:
2569 1145 749
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3980 4012 3980
Size: 32 4431
Tth111I GACn'n_nGTC
Cuts at: 2260 2260
Size: 4463
XbaI T'CTAG_A
Cuts at: 1494 1494
Size: 4463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1958 1958
Size: 4463
XmaI C'CCGG_G
Cuts at: 1483 1483
Size: 4463
XmnI GAAnn'nnTTC
Cuts at: 118 118
Size: 4463
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI