Restriction map for
pF1KB7772
(Circular) (Six-base) MAPSORT of: pF1KB7772.seq Check: 3693 from: 1 to: 4628
pF1KB7772 4628 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 1250 1250
Size: 4628
AccI GT'mk_AC
Cuts at: 1666 1666
Size: 4628
Acc65I G'GTAC_C
Cuts at: 1644 1644
Size: 4628
AclI AA'CG_TT
Cuts at: 4468 4468
Size: 4628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 1502 2453 2885 3632 3900 953
Size: 549 951 432 747 268 1681
Fragments arranged by size:
1681 951 747 549 432 268
AflIII A'CryG_T
Cuts at: 1980 3084 4061 1980
Size: 1104 977 2547
Fragments arranged by size:
2547 1104 977
AgeI A'CCGG_T
Cuts at: 3016 3016
Size: 4628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 4628
AlwNI CAG_nnn'CTG
Cuts at: 142 940 1203 1933 3500 142
Size: 798 263 730 1567 1270
Fragments arranged by size:
1567 1270 798 730 263
ApaI G_GGCC'C
Cuts at: 346 1163 346
Size: 817 3811
ApaLI G'TGCA_C
Cuts at: 3398 3398
Size: 4628
ApoI r'AATT_y
Cuts at: 126 1632 3981 4237 126
Size: 1506 2349 256 517
Fragments arranged by size:
2349 1506 517 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4628
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
AvaI C'yCGr_G
Cuts at: 250 1648 250
Size: 1398 3230
BamHI G'GATC_C
Cuts at: 111 1653 111
Size: 1542 3086
BanI G'GyrC_C
Cuts at: 781 1644 2058 2306 2341 781
Size: 863 414 248 35 3068
Fragments arranged by size:
3068 863 414 248 35
BanII G_rGCy'C
Cuts at: 346 799 1037 1163 1642 2672 346
Size: 453 238 126 479 1030 2302
Fragments arranged by size:
2302 1030 479 453 238 126
BbeI G_GCGC'C
Cuts at: 2062 2310 2062
Size: 248 4380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 294 328 294
Size: 34 4594
BciVI GTATCCnnnnn_n'
Cuts at: 2526 3287 4628 2526
Size: 761 1341 2526
Fragments arranged by size:
2526 1341 761
BclI T'GATC_A
Cuts at: 2148 2148
Size: 4628
BglI GCCn_nnn'nGGC
Cuts at: 139 321 803 139
Size: 182 482 3964
Fragments arranged by size:
3964 482 182
BglII A'GATC_T
Cuts at: 264 485 1518 2143 4180 264
Size: 221 1033 625 2037 712
Fragments arranged by size:
2037 1033 712 625 221
BlpI GC'TnA_GC
Cuts at: 546 1740 546
Size: 1194 3434
Bme1580I G_kGCm'C
Cuts at: 346 1163 1437 2253 2346 3402 346
Size: 817 274 816 93 1056 1572
Fragments arranged by size:
1572 1056 817 816 274 93
BmrI ACTGGGnnnn_n'
Cuts at: 1840 1921 2256 4400 1840
Size: 81 335 2144 2068
Fragments arranged by size:
2144 2068 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 2995 312
Size: 2683 1945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1173 1225 1305 1355 1403 1564 1173
Size: 52 80 50 48 161 4237
Fragments arranged by size:
4237 161 80 52 50 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1804 2683 3175 3473 948
Size: 856 879 492 298 2103
Fragments arranged by size:
2103 879 856 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1081 4332 1081
Size: 3251 1377
BsaAI yAC'GTr
Cuts at: 1981 1981
Size: 4628
BsaBI GATnn'nnATC
Cuts at: 6 1517 2142 4179 6
Size: 1511 625 2037 455
Fragments arranged by size:
2037 1511 625 455
BsaHI Gr'CG_yC
Cuts at: 1247 1340 2059 2307 3009 4505 1247
Size: 93 719 248 702 1496 1370
Fragments arranged by size:
1496 1370 719 702 248 93
BsaWI w'CCGG_w
Cuts at: 1817 2338 3016 3290 3437 3848 1817
Size: 521 678 274 147 411 2597
Fragments arranged by size:
2597 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1120 1150 1120
Size: 30 4598
BseYI C'CCAG_C
Cuts at: 347 1149 1302 1378 2053 2811 3388 3884 347
Size: 802 153 76 675 758 577 496 1091
Fragments arranged by size:
1091 802 758 675 577 496 153 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 571 183
Size: 388 4240
BsiEI CG_ry'CG
Cuts at: 104 338 2170 2216 3424 104
Size: 234 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 234 46
BsiHKAI G_wGCw'C
Cuts at: 1469 1642 2420 2610 3402 1469
Size: 173 778 190 792 2695
Fragments arranged by size:
2695 792 778 190 173
BsmI GAATG_Cn'
Cuts at: 408 1508 408
Size: 1100 3528
Bsp1286I G_dGCh'C
Cuts at: 346 799 1037 1163 1437 1469 1642 2253 2346
Size: 453 238 126 274 32 173 611 93
Cuts at: 2346 2420 2610 2672 3402 346
Size: 74 190 62 730 1572
Fragments arranged by size:
1572 730 611 453 274 238 190 173
126 93 74 62 32
BspEI T'CCGG_A
Cuts at: 3848 3848
Size: 4628
BspHI T'CATG_A
Cuts at: 1253 3804 1253
Size: 2551 2077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1678 1892 2194 2575 1678
Size: 214 302 381 3731
Fragments arranged by size:
3731 381 302 214
BsrBI CCG'CTC
Cuts at: 50 194 668 2920 2974 4458 4623 50
Size: 144 474 2252 54 1484 165 55
Fragments arranged by size:
2252 1484 474 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 2540 2540
Size: 4628
BsrFI r'CCGG_y
Cuts at: 333 622 1111 2626 2807 3016 333
Size: 289 489 1515 181 209 1945
Fragments arranged by size:
1945 1515 489 289 209 181
BssSI C'ACGA_G
Cuts at: 2899 3257 2899
Size: 358 4270
BstAPI GCAn_nnn'nTGC
Cuts at: 833 2005 833
Size: 1172 3456
BstBI TT'CG_AA
Cuts at: 173 2989 173
Size: 2816 1812
BstEII G'GTnAC_C
Cuts at: 1107 1107
Size: 4628
BstXI CCAn_nnnn'nTGG
Cuts at: 1329 1386 1452 1329
Size: 57 66 4505
Fragments arranged by size:
4505 66 57
BstYI r'GATC_y
Cuts at: 1 111 162 264 485 1266 1512 1518 1617
Size: 110 51 102 221 781 246 6 99
Cuts at: 1617 1653 2120 2143 2478 2724 3736 3822 3834
Size: 36 467 23 335 246 1012 86 12
Cuts at: 3834 4180 1
Size: 346 449
Fragments arranged by size:
1012 781 467 449 346 335 246 246
221 110 102 99 86 51 36 23
12 6
Bsu36I CC'TnA_GG
Cuts at: 653 1508 653
Size: 855 3773
BtgI C'CryG_G
Cuts at: 107 698 1334 107
Size: 591 636 3401
Fragments arranged by size:
3401 636 591
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2578 2759 2922 2578
Size: 181 163 4284
Fragments arranged by size:
4284 181 163
BtsI GCAGTG_nn'
Cuts at: 909 1871 909
Size: 962 3666
DraI TTT'AAA
Cuts at: 1627 1627
Size: 4628
DrdI GACnn_nn'nnGTC
Cuts at: 2334 3192 2334
Size: 858 3770
EaeI y'GGCC_r
Cuts at: 335 804 1331 2213 2387 2778 2805 4150 335
Size: 469 527 882 174 391 27 1345 813
Fragments arranged by size:
1345 882 813 527 469 391 174 27
EagI C'GGCC_G
Cuts at: 335 2213 335
Size: 1878 2750
EarI CTCTTCn'nnn_
Cuts at: 1053 1298 2651 2861 1053
Size: 245 1353 210 2820
Fragments arranged by size:
2820 1353 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 559 967 2836 3146 3292 4438 559
Size: 408 1869 310 146 1146 749
Fragments arranged by size:
1869 1146 749 408 310 146
EcoICRI GAG'CTC
Cuts at: 1640 1640
Size: 4628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 953 1173 1225 1305 1355 1403 1502 1564 2453
Size: 220 52 80 50 48 99 62 889
Cuts at: 2453 2885 3632 3900 953
Size: 432 747 268 1681
Fragments arranged by size:
1681 889 747 432 268 220 99 80
62 52 50 48
EcoNI CCTnn'n_nnAGG
Cuts at: 656 656
Size: 4628
EcoO109I rG'GnC_Cy
Cuts at: 342 343 376 1159 1160 1209 1375 1767 342
Size: 1 33 783 1 49 166 392 3203
Fragments arranged by size:
3203 783 392 166 49 33 1 1
EcoRI G'AATT_C
Cuts at: 126 1632 126
Size: 1506 3122
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 4628
FspI TGC'GCA
Cuts at: 2409 2409
Size: 4628
HaeII r_GCGC'y
Cuts at: 297 458 2062 2310 3332 4311 297
Size: 161 1604 248 1022 979 614
Fragments arranged by size:
1604 1022 979 614 248 161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 209 241 2470 2500 2502 2532 209
Size: 32 2229 30 2 30 2305
Fragments arranged by size:
2305 2229 32 30 30 2
HincII GTy'rAC
Cuts at: 1667 1667
Size: 4628
KasI G'GCGC_C
Cuts at: 2058 2306 2058
Size: 248 4380
KpnI G_GTAC'C
Cuts at: 1648 1648
Size: 4628
MluI A'CGCG_T
Cuts at: 4061 4061
Size: 4628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 814 1312 3299 3483 814
Size: 498 1987 184 1959
Fragments arranged by size:
1987 1959 498 184
MscI TGG'CCA
Cuts at: 1333 2389 1333
Size: 1056 3572
MslI CAynn'nnrTG
Cuts at: 2617 2744 2617
Size: 127 4501
MspA1I CmG'CkG
Cuts at: 1739 2053 2413 3426 3671 1739
Size: 314 360 1013 245 2696
Fragments arranged by size:
2696 1013 360 314 245
NaeI GCC'GGC
Cuts at: 335 2809 335
Size: 2474 2154
NarI GG'CG_CC
Cuts at: 2059 2307 2059
Size: 248 4380
NcoI C'CATG_G
Cuts at: 107 698 107
Size: 591 4037
NdeI CA'TA_TG
Cuts at: 423 423
Size: 4628
NgoMIV G'CCGG_C
Cuts at: 333 2807 333
Size: 2474 2154
NspI r_CATG'y
Cuts at: 1681 2181 3088 1681
Size: 500 907 3221
Fragments arranged by size:
3221 907 500
PciI A'CATG_T
Cuts at: 3084 3084
Size: 4628
PflMI CCAn_nnn'nTGG
Cuts at: 1203 1203
Size: 4628
PmeI GTTT'AAAC
Cuts at: 1627 1627
Size: 4628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 4596
PpuMI rG'GwC_Cy
Cuts at: 376 376
Size: 4628
PspOMI G'GGCC_C
Cuts at: 342 1159 342
Size: 817 3811
PstI C_TGCA'G
Cuts at: 1675 2360 1675
Size: 685 3943
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4628
PvuII CAG'CTG
Cuts at: 2053 2413 2053
Size: 360 4268
RsrII CG'GwC_CG
Cuts at: 2823 2823
Size: 4628
SacI G_AGCT'C
Cuts at: 1642 1642
Size: 4628
SalI G'TCGA_C
Cuts at: 1665 1665
Size: 4628
SapI GCTCTTCn'nnn_
Cuts at: 2651 2861 2651
Size: 210 4418
SbfI CC_TGCA'GG
Cuts at: 1675 1675
Size: 4628
ScaI AGT'ACT
Cuts at: 4145 4145
Size: 4628
SfcI C'TryA_G
Cuts at: 33 1128 1671 2356 3349 3540 33
Size: 1095 543 685 993 191 1121
Fragments arranged by size:
1121 1095 993 685 543 191
SfoI GGC'GCC
Cuts at: 2060 2308 2060
Size: 248 4380
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4628
SmaI CCC'GGG
Cuts at: 1650 1650
Size: 4628
SmlI C'TyrA_G
Cuts at: 927 1783 2698 3190 3452 927
Size: 856 915 492 262 2103
Fragments arranged by size:
2103 915 856 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4628
SphI G_CATG'C
Cuts at: 1681 2181 1681
Size: 500 4128
StyI C'CwwG_G
Cuts at: 107 698 1445 1762 2116 107
Size: 591 747 317 354 2619
Fragments arranged by size:
2619 747 591 354 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2211 2344 2803 3012 2211
Size: 133 459 209 3827
Fragments arranged by size:
3827 459 209 133
TatI w'GTAC_w
Cuts at: 4143 4143
Size: 4628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 565 597 861 893 4145 4177 565
Size: 32 264 32 3252 32 1016
Fragments arranged by size:
3252 1016 264 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2425 2425
Size: 4628
XbaI T'CTAG_A
Cuts at: 1017 1515 1659 1017
Size: 498 144 3986
Fragments arranged by size:
3986 498 144
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2123 2123
Size: 4628
XmaI C'CCGG_G
Cuts at: 1648 1648
Size: 4628
XmnI GAAnn'nnTTC
Cuts at: 888 888
Size: 4628
ZraI GAC'GTC
Cuts at: 1248 1248
Size: 4628
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BseRI
BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI