Restriction map for
pF1KB7774
(Circular) (Six-base) MAPSORT of: pF1KB7774.seq Check: 6210 from: 1 to: 4631
pF1KB7774 4631 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1669 1669
Size: 4631
Acc65I G'GTAC_C
Cuts at: 1647 1647
Size: 4631
AclI AA'CG_TT
Cuts at: 4471 4471
Size: 4631
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1359 2456 2888 3635 3903 687
Size: 672 1097 432 747 268 1415
Fragments arranged by size:
1415 1097 747 672 432 268
AflIII A'CryG_T
Cuts at: 1983 3087 4064 1983
Size: 1104 977 2550
Fragments arranged by size:
2550 1104 977
AgeI A'CCGG_T
Cuts at: 3019 3019
Size: 4631
AleI CACnn'nnGTG
Cuts at: 417 1082 1166 417
Size: 665 84 3882
Fragments arranged by size:
3882 665 84
AlwNI CAG_nnn'CTG
Cuts at: 1936 3503 1936
Size: 1567 3064
ApaLI G'TGCA_C
Cuts at: 1213 3401 1213
Size: 2188 2443
ApoI r'AATT_y
Cuts at: 1073 1117 1241 1493 1635 3984 4240 1073
Size: 44 124 252 142 2349 256 1464
Fragments arranged by size:
2349 1464 256 252 142 124 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4631
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4631
AvaI C'yCGr_G
Cuts at: 1651 1651
Size: 4631
BamHI G'GATC_C
Cuts at: 1229 1656 1229
Size: 427 4204
BanI G'GyrC_C
Cuts at: 1647 2061 2309 2344 1647
Size: 414 248 35 3934
Fragments arranged by size:
3934 414 248 35
BanII G_rGCy'C
Cuts at: 1645 2675 1645
Size: 1030 3601
BbeI G_GCGC'C
Cuts at: 2065 2313 2065
Size: 248 4383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4597
BciVI GTATCCnnnnn_n'
Cuts at: 2529 3290 4631 2529
Size: 761 1341 2529
Fragments arranged by size:
2529 1341 761
BclI T'GATC_A
Cuts at: 2151 2151
Size: 4631
BfrBI ATG'CAT
Cuts at: 1329 1329
Size: 4631
BglII A'GATC_T
Cuts at: 169 2146 4183 169
Size: 1977 2037 617
Fragments arranged by size:
2037 1977 617
BlpI GC'TnA_GC
Cuts at: 1743 1743
Size: 4631
Bme1580I G_kGCm'C
Cuts at: 1217 2256 2349 3405 1217
Size: 1039 93 1056 2443
Fragments arranged by size:
2443 1056 1039 93
BmrI ACTGGGnnnn_n'
Cuts at: 1843 1924 2259 4403 1843
Size: 81 335 2144 2071
Fragments arranged by size:
2144 2071 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 399 2998 399
Size: 2599 2032
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 851 298
Size: 553 4078
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1807 2686 3178 3476 1807
Size: 879 492 298 2962
Fragments arranged by size:
2962 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 274 663 831 1503 4335 274
Size: 389 168 672 2832 570
Fragments arranged by size:
2832 672 570 389 168
BsaAI yAC'GTr
Cuts at: 1984 1984
Size: 4631
BsaBI GATnn'nnATC
Cuts at: 6 2145 4182 6
Size: 2139 2037 455
Fragments arranged by size:
2139 2037 455
BsaHI Gr'CG_yC
Cuts at: 2062 2310 3012 4508 2062
Size: 248 702 1496 2185
Fragments arranged by size:
2185 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1403 1820 2341 3019 3293 3440 3851 1403
Size: 417 521 678 274 147 411 2183
Fragments arranged by size:
2183 678 521 417 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 365 388 593 365
Size: 23 205 4403
Fragments arranged by size:
4403 205 23
BseYI C'CCAG_C
Cuts at: 2056 2814 3391 3887 2056
Size: 758 577 496 2800
Fragments arranged by size:
2800 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2173 2219 3427 104
Size: 2069 46 1208 1308
Fragments arranged by size:
2069 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1217 1645 2423 2613 3405 1217
Size: 428 778 190 792 2443
Fragments arranged by size:
2443 792 778 428 190
BsmI GAATG_Cn'
Cuts at: 685 1329 685
Size: 644 3987
Bsp1286I G_dGCh'C
Cuts at: 1217 1645 2256 2349 2423 2613 2675 3405 1217
Size: 428 611 93 74 190 62 730 2443
Fragments arranged by size:
2443 730 611 428 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3851 3851
Size: 4631
BspHI T'CATG_A
Cuts at: 600 3807 600
Size: 3207 1424
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1681 1895 2197 2578 1681
Size: 214 302 381 3734
Fragments arranged by size:
3734 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2923 2977 4461 4626 50
Size: 2873 54 1484 165 55
Fragments arranged by size:
2873 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 432 722 2543 432
Size: 290 1821 2520
Fragments arranged by size:
2520 1821 290
BsrFI r'CCGG_y
Cuts at: 2629 2810 3019 2629
Size: 181 209 4241
Fragments arranged by size:
4241 209 181
BsrGI T'GTAC_A
Cuts at: 1603 1603
Size: 4631
BssSI C'ACGA_G
Cuts at: 2902 3260 2902
Size: 358 4273
BstAPI GCAn_nnn'nTGC
Cuts at: 2008 2008
Size: 4631
BstBI TT'CG_AA
Cuts at: 2992 2992
Size: 4631
BstYI r'GATC_y
Cuts at: 1 169 403 1229 1656 2123 2146 2481 2727
Size: 168 234 826 427 467 23 335 246
Cuts at: 2727 3739 3825 3837 4183 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 826 467 449 427 346 335 246
234 168 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 384 451 384
Size: 67 4564
BtgI C'CryG_G
Cuts at: 390 390
Size: 4631
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2581 2762 2925 2581
Size: 181 163 4287
Fragments arranged by size:
4287 181 163
BtsI GCAGTG_nn'
Cuts at: 412 1874 412
Size: 1462 3169
DraI TTT'AAA
Cuts at: 1630 1630
Size: 4631
DrdI GACnn_nn'nnGTC
Cuts at: 2337 3195 2337
Size: 858 3773
EaeI y'GGCC_r
Cuts at: 317 2216 2390 2781 2808 4153 317
Size: 1899 174 391 27 1345 795
Fragments arranged by size:
1899 1345 795 391 174 27
EagI C'GGCC_G
Cuts at: 2216 2216
Size: 4631
EarI CTCTTCn'nnn_
Cuts at: 350 2654 2864 350
Size: 2304 210 2117
Fragments arranged by size:
2304 2117 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2839 3149 3295 4441 2839
Size: 310 146 1146 3029
Fragments arranged by size:
3029 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1643 1643
Size: 4631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 687 851 1359 2456 2888 3635 3903 298
Size: 389 164 508 1097 432 747 268 1026
Fragments arranged by size:
1097 1026 747 508 432 389 268 164
EcoO109I rG'GnC_Cy
Cuts at: 755 1770 755
Size: 1015 3616
EcoRI G'AATT_C
Cuts at: 1073 1241 1493 1635 1073
Size: 168 252 142 4069
Fragments arranged by size:
4069 252 168 142
EcoRV GAT'ATC
Cuts at: 968 968
Size: 4631
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4631
FspI TGC'GCA
Cuts at: 2412 2412
Size: 4631
HaeII r_GCGC'y
Cuts at: 2065 2313 3335 4314 2065
Size: 248 1022 979 2382
Fragments arranged by size:
2382 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 704 736 2473 2503 2505 2535 364
Size: 32 308 32 1737 30 2 30 2460
Fragments arranged by size:
2460 1737 308 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1399 1670 1399
Size: 271 4360
HindIII A'AGCT_T
Cuts at: 614 1538 614
Size: 924 3707
KasI G'GCGC_C
Cuts at: 2061 2309 2061
Size: 248 4383
KpnI G_GTAC'C
Cuts at: 1651 1651
Size: 4631
MluI A'CGCG_T
Cuts at: 4064 4064
Size: 4631
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 3302 3486 323
Size: 2979 184 1468
Fragments arranged by size:
2979 1468 184
MscI TGG'CCA
Cuts at: 319 2392 319
Size: 2073 2558
MslI CAynn'nnrTG
Cuts at: 266 417 461 605 1082 1166 2620 2747 266
Size: 151 44 144 477 84 1454 127 2150
Fragments arranged by size:
2150 1454 477 151 144 127 84 44
MspA1I CmG'CkG
Cuts at: 1742 2056 2416 3429 3674 1742
Size: 314 360 1013 245 2699
Fragments arranged by size:
2699 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2812 2812
Size: 4631
NarI GG'CG_CC
Cuts at: 2062 2310 2062
Size: 248 4383
NcoI C'CATG_G
Cuts at: 390 390
Size: 4631
NgoMIV G'CCGG_C
Cuts at: 2810 2810
Size: 4631
NsiI A_TGCA'T
Cuts at: 1331 1331
Size: 4631
NspI r_CATG'y
Cuts at: 1684 2184 3091 1684
Size: 500 907 3224
Fragments arranged by size:
3224 907 500
PciI A'CATG_T
Cuts at: 3087 3087
Size: 4631
PflMI CCAn_nnn'nTGG
Cuts at: 118 118
Size: 4631
PfoI T'CCnGG_A
Cuts at: 153 153
Size: 4631
PmeI GTTT'AAAC
Cuts at: 1630 1630
Size: 4631
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 3770
Size: 32 4599
PstI C_TGCA'G
Cuts at: 1678 2363 1678
Size: 685 3946
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4631
PvuII CAG'CTG
Cuts at: 2056 2416 2056
Size: 360 4271
RsrII CG'GwC_CG
Cuts at: 2826 2826
Size: 4631
SacI G_AGCT'C
Cuts at: 1645 1645
Size: 4631
SalI G'TCGA_C
Cuts at: 1668 1668
Size: 4631
SapI GCTCTTCn'nnn_
Cuts at: 350 2654 2864 350
Size: 2304 210 2117
Fragments arranged by size:
2304 2117 210
SbfI CC_TGCA'GG
Cuts at: 1678 1678
Size: 4631
ScaI AGT'ACT
Cuts at: 4148 4148
Size: 4631
SfcI C'TryA_G
Cuts at: 33 285 621 1674 2359 3352 3543 33
Size: 252 336 1053 685 993 191 1121
Fragments arranged by size:
1121 1053 993 685 336 252 191
SfoI GGC'GCC
Cuts at: 2063 2311 2063
Size: 248 4383
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4631
SmaI CCC'GGG
Cuts at: 1653 1653
Size: 4631
SmlI C'TyrA_G
Cuts at: 1786 2701 3193 3455 1786
Size: 915 492 262 2962
Fragments arranged by size:
2962 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4631
SphI G_CATG'C
Cuts at: 1684 2184 1684
Size: 500 4131
StyI C'CwwG_G
Cuts at: 390 1765 2119 390
Size: 1375 354 2902
Fragments arranged by size:
2902 1375 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 588 2214 2347 2806 3015 588
Size: 1626 133 459 209 2204
Fragments arranged by size:
2204 1626 459 209 133
TatI w'GTAC_w
Cuts at: 850 1603 4146 850
Size: 753 2543 1335
Fragments arranged by size:
2543 1335 753
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 791 823 4148 4180 791
Size: 32 3325 32 1242
Fragments arranged by size:
3325 1242 32 32
Tth111I GACn'n_nGTC
Cuts at: 2428 2428
Size: 4631
XbaI T'CTAG_A
Cuts at: 1662 1662
Size: 4631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2126 2126
Size: 4631
XmaI C'CCGG_G
Cuts at: 1651 1651
Size: 4631
XmnI GAAnn'nnTTC
Cuts at: 532 1036 1120 1204 1372 1540 532
Size: 504 84 84 168 168 3623
Fragments arranged by size:
3623 504 168 168 84 84
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI