Restriction map for
pF1KB7777
(Circular) (Six-base) MAPSORT of: pF1KB7777.seq Check: 1248 from: 1 to: 4472
pF1KB7777 4472 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 1053 1053
Size: 4472
AccI GT'mk_AC
Cuts at: 663 1173 1510 663
Size: 510 337 3625
Fragments arranged by size:
3625 510 337
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 2729 3476 3744 2297
Size: 432 747 268 3025
Fragments arranged by size:
3025 747 432 268
AflIII A'CryG_T
Cuts at: 1157 1824 2928 3905 1157
Size: 667 1104 977 1724
Fragments arranged by size:
1724 1104 977 667
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AhdI GACnn_n'nnGTC
Cuts at: 1202 1202
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 346 1346 1777 3344 346
Size: 1000 431 1567 1474
Fragments arranged by size:
1567 1474 1000 431
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 317 979 1476 3825 4081 317
Size: 662 497 2349 256 708
Fragments arranged by size:
2349 708 662 497 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 713 1492 713
Size: 779 3693
BamHI G'GATC_C
Cuts at: 1497 1497
Size: 4472
BanI G'GyrC_C
Cuts at: 1488 1902 2150 2185 1488
Size: 414 248 35 3775
Fragments arranged by size:
3775 414 248 35
BanII G_rGCy'C
Cuts at: 1486 2516 1486
Size: 1030 3442
BbeI G_GCGC'C
Cuts at: 1906 2154 1906
Size: 248 4224
BbsI GAAGACnn'nnnn_
Cuts at: 520 871 1021 1466 520
Size: 351 150 445 3526
Fragments arranged by size:
3526 445 351 150
BbvCI CC'TCA_GC
Cuts at: 849 849
Size: 4472
BciVI GTATCCnnnnn_n'
Cuts at: 2370 3131 4472 2370
Size: 761 1341 2370
Fragments arranged by size:
2370 1341 761
BclI T'GATC_A
Cuts at: 1992 1992
Size: 4472
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 2097 2190 3246 2097
Size: 93 1056 3323
Fragments arranged by size:
3323 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 161 786 826 1684 1765 2100 4244 161
Size: 625 40 858 81 335 2144 389
Fragments arranged by size:
2144 858 625 389 335 81 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 726 2839 726
Size: 2113 2359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 1453
Size: 4472
Bpu10I CC'TnA_GC
Cuts at: 849 849
Size: 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1648 2527 3019 3317 1648
Size: 879 492 298 2803
Fragments arranged by size:
2803 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4176 4176
Size: 4472
BsaAI yAC'GTr
Cuts at: 1160 1825 1160
Size: 665 3807
BsaBI GATnn'nnATC
Cuts at: 6 1986 4023 6
Size: 1980 2037 455
Fragments arranged by size:
2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 1050 1903 2151 2853 4349 1050
Size: 853 248 702 1496 1173
Fragments arranged by size:
1496 1173 853 702 248
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 244 244
Size: 4472
BseYI C'CCAG_C
Cuts at: 1897 2655 3232 3728 1897
Size: 758 577 496 2641
Fragments arranged by size:
2641 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1365 2014 2060 3268 104
Size: 1261 649 46 1208 1308
Fragments arranged by size:
1308 1261 1208 649 46
BsiHKAI G_wGCw'C
Cuts at: 1486 2264 2454 3246 1486
Size: 778 190 792 2712
Fragments arranged by size:
2712 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1486 2097 2190 2264 2454 2516 3246 1486
Size: 611 93 74 190 62 730 2712
Fragments arranged by size:
2712 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 494 1522 1736 2038 2419 494
Size: 1028 214 302 381 2547
Fragments arranged by size:
2547 1028 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1288 2384 1288
Size: 1096 3376
BsrFI r'CCGG_y
Cuts at: 2470 2651 2860 2470
Size: 181 209 4082
Fragments arranged by size:
4082 209 181
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 1849
Size: 4472
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstXI CCAn_nnnn'nTGG
Cuts at: 114 561 114
Size: 447 4025
BstYI r'GATC_y
Cuts at: 1 131 1328 1497 1964 1987 2322 2568 3580
Size: 130 1197 169 467 23 335 246 1012
Cuts at: 3580 3666 3678 4024 1
Size: 86 12 346 449
Fragments arranged by size:
1197 1012 467 449 346 335 246 169
130 86 23 12
BtgI C'CryG_G
Cuts at: 107 209 107
Size: 102 4370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2422 2603 2766 2422
Size: 181 163 4128
Fragments arranged by size:
4128 181 163
BtsI GCAGTG_nn'
Cuts at: 1715 1715
Size: 4472
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3036 2178
Size: 858 3614
EaeI y'GGCC_r
Cuts at: 563 1072 1362 1394 2057 2231 2622 2649 3994
Size: 509 290 32 663 174 391 27 1345
Cuts at: 3994 563
Size: 1041
Fragments arranged by size:
1345 1041 663 509 391 290 174 32
27
EagI C'GGCC_G
Cuts at: 1362 2057 1362
Size: 695 3777
EarI CTCTTCn'nnn_
Cuts at: 173 299 455 983 1088 2495 2705 173
Size: 126 156 528 105 1407 210 1940
Fragments arranged by size:
1940 1407 528 210 156 126 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 817 2680 2990 3136 4282 817
Size: 1863 310 146 1146 1007
Fragments arranged by size:
1863 1146 1007 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 2297 2729 3476 3744 1453
Size: 844 432 747 268 2181
Fragments arranged by size:
2181 844 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1611 1611
Size: 4472
EcoRI G'AATT_C
Cuts at: 1476 1476
Size: 4472
EcoRV GAT'ATC
Cuts at: 1086 1086
Size: 4472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 802 2491 802
Size: 1689 2783
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 1906 2154 3176 4155 1906
Size: 248 1022 979 2223
Fragments arranged by size:
2223 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 322 354 676 708 1118 1150 1188 1220 1267
Size: 32 322 32 410 32 38 32 47
Cuts at: 1267 1299 2314 2344 2346 2376 322
Size: 32 1015 30 2 30 2418
Fragments arranged by size:
2418 1015 410 322 47 38 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1174 1511 1174
Size: 337 4135
KasI G'GCGC_C
Cuts at: 1902 2150 1902
Size: 248 4224
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MfeI C'AATT_G
Cuts at: 1019 1335 1019
Size: 316 4156
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1186 1330 3143 3327 1186
Size: 144 1813 184 2331
Fragments arranged by size:
2331 1813 184 144
MscI TGG'CCA
Cuts at: 565 1396 2233 565
Size: 831 837 2804
Fragments arranged by size:
2804 837 831
MslI CAynn'nnrTG
Cuts at: 2461 2588 2461
Size: 127 4345
MspA1I CmG'CkG
Cuts at: 1367 1583 1897 2257 3270 3515 1367
Size: 216 314 360 1013 245 2324
Fragments arranged by size:
2324 1013 360 314 245 216
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 1903 2151 1903
Size: 248 4224
NcoI C'CATG_G
Cuts at: 107 209 107
Size: 102 4370
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NspI r_CATG'y
Cuts at: 1525 2025 2932 1525
Size: 500 907 3065
Fragments arranged by size:
3065 907 500
PciI A'CATG_T
Cuts at: 2928 2928
Size: 4472
PflMI CCAn_nnn'nTGG
Cuts at: 798 798
Size: 4472
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PmlI CAC'GTG
Cuts at: 1160 1160
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 188 220 3611 3643 188
Size: 32 3391 32 1017
Fragments arranged by size:
3391 1017 32 32
PstI C_TGCA'G
Cuts at: 1519 2204 1519
Size: 685 3787
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 1897 2257 1897
Size: 360 4112
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1172 1509 1172
Size: 337 4135
SapI GCTCTTCn'nnn_
Cuts at: 299 2495 2705 299
Size: 2196 210 2066
Fragments arranged by size:
2196 2066 210
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 3989 3989
Size: 4472
SfcI C'TryA_G
Cuts at: 33 660 1515 2200 3193 3384 33
Size: 627 855 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 627 191
SfoI GGC'GCC
Cuts at: 1904 2152 1904
Size: 248 4224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 1627 2542 3034 3296 1627
Size: 915 492 262 2803
Fragments arranged by size:
2803 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 1525 2025 1525
Size: 500 3972
StyI C'CwwG_G
Cuts at: 107 209 1606 1960 107
Size: 102 1397 354 2619
Fragments arranged by size:
2619 1397 354 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1296 2055 2188 2647 2856 1296
Size: 759 133 459 209 2912
Fragments arranged by size:
2912 759 459 209 133
TatI w'GTAC_w
Cuts at: 1040 3987 1040
Size: 2947 1525
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1294 1326 3989 4021 1294
Size: 32 2663 32 1745
Fragments arranged by size:
2663 1745 32 32
Tth111I GACn'n_nGTC
Cuts at: 1049 2269 1049
Size: 1220 3252
XbaI T'CTAG_A
Cuts at: 508 951 1503 508
Size: 443 552 3477
Fragments arranged by size:
3477 552 443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 558 1219 1967 558
Size: 661 748 3063
Fragments arranged by size:
3063 748 661
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
ZraI GAC'GTC
Cuts at: 1051 1051
Size: 4472
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI