Restriction map for
pF1KB7778
(Circular) (Six-base) MAPSORT of: pF1KB7778.seq Check: 9716 from: 1 to: 4637
pF1KB7778 4637 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1422 1422
Size: 4637
AatII G_ACGT'C
Cuts at: 1297 1297
Size: 4637
AccI GT'mk_AC
Cuts at: 691 1675 691
Size: 984 3653
Acc65I G'GTAC_C
Cuts at: 1653 1653
Size: 4637
AclI AA'CG_TT
Cuts at: 4477 4477
Size: 4637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 573 916 1096 2462 2894 3641 3909 421
Size: 152 343 180 1366 432 747 268 1149
Fragments arranged by size:
1366 1149 747 432 343 268 180 152
AflIII A'CryG_T
Cuts at: 1989 3093 4070 1989
Size: 1104 977 2556
Fragments arranged by size:
2556 1104 977
AgeI A'CCGG_T
Cuts at: 3025 3025
Size: 4637
AhdI GACnn_n'nnGTC
Cuts at: 357 357
Size: 4637
AleI CACnn'nnGTG
Cuts at: 1312 1312
Size: 4637
AlwNI CAG_nnn'CTG
Cuts at: 657 1027 1942 3509 657
Size: 370 915 1567 1785
Fragments arranged by size:
1785 1567 915 370
ApaI G_GGCC'C
Cuts at: 266 330 449 266
Size: 64 119 4454
Fragments arranged by size:
4454 119 64
ApaLI G'TGCA_C
Cuts at: 1192 3407 1192
Size: 2215 2422
ApoI r'AATT_y
Cuts at: 1641 3990 4246 1641
Size: 2349 256 2032
Fragments arranged by size:
2349 2032 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4637
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
AvaI C'yCGr_G
Cuts at: 241 259 448 527 1368 1657 241
Size: 18 189 79 841 289 3221
Fragments arranged by size:
3221 841 289 189 79 18
BamHI G'GATC_C
Cuts at: 1662 1662
Size: 4637
BanI G'GyrC_C
Cuts at: 312 382 1017 1416 1653 2067 2315 2350 312
Size: 70 635 399 237 414 248 35 2599
Fragments arranged by size:
2599 635 414 399 248 237 70 35
BanII G_rGCy'C
Cuts at: 154 260 266 330 449 1651 2681 154
Size: 106 6 64 119 1202 1030 2110
Fragments arranged by size:
2110 1202 1030 119 106 64 6
BbeI G_GCGC'C
Cuts at: 316 386 2071 2319 316
Size: 70 1685 248 2634
Fragments arranged by size:
2634 1685 248 70
BbsI GAAGACnn'nnnn_
Cuts at: 529 607 529
Size: 78 4559
BciVI GTATCCnnnnn_n'
Cuts at: 2535 3296 4637 2535
Size: 761 1341 2535
Fragments arranged by size:
2535 1341 761
BclI T'GATC_A
Cuts at: 722 2157 722
Size: 1435 3202
BglI GCCn_nnn'nGGC
Cuts at: 136 136
Size: 4637
BglII A'GATC_T
Cuts at: 840 2152 4189 840
Size: 1312 2037 1288
Fragments arranged by size:
2037 1312 1288
BlpI GC'TnA_GC
Cuts at: 1749 1749
Size: 4637
Bme1580I G_kGCm'C
Cuts at: 266 330 449 1196 1307 2262 2355 3411 266
Size: 64 119 747 111 955 93 1056 1492
Fragments arranged by size:
1492 1056 955 747 119 111 93 64
BmrI ACTGGGnnnn_n'
Cuts at: 1037 1168 1849 1930 2265 4409 1037
Size: 131 681 81 335 2144 1265
Fragments arranged by size:
2144 1265 681 335 131 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 369 3004 369
Size: 2635 2002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1242 295
Size: 947 3690
Bpu10I CC'TnA_GC
Cuts at: 412 412
Size: 4637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1813 2692 3184 3482 1813
Size: 879 492 298 2968
Fragments arranged by size:
2968 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 727 874 4341 727
Size: 147 3467 1023
Fragments arranged by size:
3467 1023 147
BsaAI yAC'GTr
Cuts at: 1990 1990
Size: 4637
BsaBI GATnn'nnATC
Cuts at: 6 424 2151 4188 6
Size: 418 1727 2037 455
Fragments arranged by size:
2037 1727 455 418
BsaHI Gr'CG_yC
Cuts at: 313 383 454 1294 1466 2068 2316 3018 4514
Size: 70 71 840 172 602 248 702 1496
Cuts at: 4514 313
Size: 436
Fragments arranged by size:
1496 840 702 602 436 248 172 71
70
BsaWI w'CCGG_w
Cuts at: 176 1826 2347 3025 3299 3446 3857 176
Size: 1650 521 678 274 147 411 956
Fragments arranged by size:
1650 956 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 385 388 1092 1245 382
Size: 3 3 704 153 3774
Fragments arranged by size:
3774 704 153 3 3
BseYI C'CCAG_C
Cuts at: 124 378 1163 2062 2820 3397 3893 124
Size: 254 785 899 758 577 496 868
Fragments arranged by size:
899 868 785 758 577 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 1525 1198
Size: 327 4310
BsiEI CG_ry'CG
Cuts at: 104 196 270 2179 2225 3433 104
Size: 92 74 1909 46 1208 1308
Fragments arranged by size:
1909 1308 1208 92 74 46
BsiHKAI G_wGCw'C
Cuts at: 1196 1651 2429 2619 3411 1196
Size: 455 778 190 792 2422
Fragments arranged by size:
2422 792 778 455 190
BsmI GAATG_Cn'
Cuts at: 1407 1407
Size: 4637
BsmBI CGTCTCn'nnnn_
Cuts at: 1220 1220
Size: 4637
Bsp1286I G_dGCh'C
Cuts at: 154 260 266 330 449 1196 1307 1651 2262
Size: 106 6 64 119 747 111 344 611
Cuts at: 2262 2355 2429 2619 2681 3411 154
Size: 93 74 190 62 730 1380
Fragments arranged by size:
1380 747 730 611 344 190 119 111
106 93 74 64 62 6
BspEI T'CCGG_A
Cuts at: 3857 3857
Size: 4637
BspHI T'CATG_A
Cuts at: 611 1289 3813 611
Size: 678 2524 1435
Fragments arranged by size:
2524 1435 678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 766 929 1158 1422 1687 1901 2203 2584 766
Size: 163 229 264 265 214 302 381 2819
Fragments arranged by size:
2819 381 302 265 264 229 214 163
BsrBI CCG'CTC
Cuts at: 50 279 390 491 503 2929 2983 4467 4632
Size: 229 111 101 12 2426 54 1484 165
Cuts at: 4632 50
Size: 55
Fragments arranged by size:
2426 1484 229 165 111 101 55 54
12
BsrDI GCAATG_nn'
Cuts at: 2549 2549
Size: 4637
BsrFI r'CCGG_y
Cuts at: 483 736 900 2635 2816 3025 483
Size: 253 164 1735 181 209 2095
Fragments arranged by size:
2095 1735 253 209 181 164
BssSI C'ACGA_G
Cuts at: 2908 3266 2908
Size: 358 4279
BstAPI GCAn_nnn'nTGC
Cuts at: 2014 2014
Size: 4637
BstBI TT'CG_AA
Cuts at: 2998 2998
Size: 4637
BstXI CCAn_nnnn'nTGG
Cuts at: 1014 1314 1014
Size: 300 4337
BstYI r'GATC_y
Cuts at: 1 840 1508 1662 2129 2152 2487 2733 3745
Size: 839 668 154 467 23 335 246 1012
Cuts at: 3745 3831 3843 4189 1
Size: 86 12 346 449
Fragments arranged by size:
1012 839 668 467 449 346 335 246
154 86 23 12
BstZ17I GTA'TAC
Cuts at: 692 692
Size: 4637
BtgI C'CryG_G
Cuts at: 131 320 131
Size: 189 4448
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2587 2768 2931 2587
Size: 181 163 4293
Fragments arranged by size:
4293 181 163
BtsI GCAGTG_nn'
Cuts at: 879 1062 1163 1880 879
Size: 183 101 717 3636
Fragments arranged by size:
3636 717 183 101
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 810 845 810
Size: 35 4602
DraI TTT'AAA
Cuts at: 1636 1636
Size: 4637
DrdI GACnn_nn'nnGTC
Cuts at: 2343 3201 2343
Size: 858 3779
EaeI y'GGCC_r
Cuts at: 193 267 464 668 1320 2222 2396 2787 2814
Size: 74 197 204 652 902 174 391 27
Cuts at: 2814 4159 193
Size: 1345 671
Fragments arranged by size:
1345 902 671 652 391 204 197 174
74 27
EagI C'GGCC_G
Cuts at: 193 267 2222 193
Size: 74 1955 2608
Fragments arranged by size:
2608 1955 74
EarI CTCTTCn'nnn_
Cuts at: 827 1072 2660 2870 827
Size: 245 1588 210 2594
Fragments arranged by size:
2594 1588 245 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2845 3155 3301 4447 2845
Size: 310 146 1146 3035
Fragments arranged by size:
3035 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1649 1649
Size: 4637
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 421 573 916 1096 1242 2462 2894 3641
Size: 126 152 343 180 146 1220 432 747
Cuts at: 3641 3909 295
Size: 268 1023
Fragments arranged by size:
1220 1023 747 432 343 268 180 152
146 126
EcoO109I rG'GnC_Cy
Cuts at: 237 326 327 1110 1170 1200 1776 237
Size: 89 1 783 60 30 576 3098
Fragments arranged by size:
3098 783 576 89 60 30 1
EcoRI G'AATT_C
Cuts at: 1641 1641
Size: 4637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2656 2656
Size: 4637
FspI TGC'GCA
Cuts at: 2418 2418
Size: 4637
HaeII r_GCGC'y
Cuts at: 316 386 579 2071 2319 3341 4320 316
Size: 70 193 1492 248 1022 979 633
Fragments arranged by size:
1492 1022 979 633 248 193 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 2479 2509 2511 2541 343
Size: 32 2104 30 2 30 2439
Fragments arranged by size:
2439 2104 32 30 30 2
HincII GTy'rAC
Cuts at: 1676 1676
Size: 4637
KasI G'GCGC_C
Cuts at: 312 382 2067 2315 312
Size: 70 1685 248 2634
Fragments arranged by size:
2634 1685 248 70
KpnI G_GTAC'C
Cuts at: 1657 1657
Size: 4637
MluI A'CGCG_T
Cuts at: 4070 4070
Size: 4637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 839 3308 3492 839
Size: 2469 184 1984
Fragments arranged by size:
2469 1984 184
MscI TGG'CCA
Cuts at: 670 1322 2398 670
Size: 652 1076 2909
Fragments arranged by size:
2909 1076 652
MslI CAynn'nnrTG
Cuts at: 1141 1189 1288 1312 1432 2626 2753 1141
Size: 48 99 24 120 1194 127 3025
Fragments arranged by size:
3025 1194 127 120 99 48 24
MspA1I CmG'CkG
Cuts at: 133 295 322 338 463 986 1091 1122 1262
Size: 162 27 16 125 523 105 31 140
Cuts at: 1262 1748 2062 2422 3435 3680 133
Size: 486 314 360 1013 245 1090
Fragments arranged by size:
1090 1013 523 486 360 314 245 162
140 125 105 31 27 16
NaeI GCC'GGC
Cuts at: 902 2818 902
Size: 1916 2721
NarI GG'CG_CC
Cuts at: 313 383 2068 2316 313
Size: 70 1685 248 2634
Fragments arranged by size:
2634 1685 248 70
NgoMIV G'CCGG_C
Cuts at: 900 2816 900
Size: 1916 2721
NspI r_CATG'y
Cuts at: 1348 1690 2190 3097 1348
Size: 342 500 907 2888
Fragments arranged by size:
2888 907 500 342
PasI CC'CwG_GG
Cuts at: 159 159
Size: 4637
PciI A'CATG_T
Cuts at: 3093 3093
Size: 4637
PflMI CCAn_nnn'nTGG
Cuts at: 1539 1539
Size: 4637
PfoI T'CCnGG_A
Cuts at: 1451 1451
Size: 4637
PmeI GTTT'AAAC
Cuts at: 1636 1636
Size: 4637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 4605
PpuMI rG'GwC_Cy
Cuts at: 237 1170 237
Size: 933 3704
PspOMI G'GGCC_C
Cuts at: 262 326 445 262
Size: 64 119 4454
Fragments arranged by size:
4454 119 64
PstI C_TGCA'G
Cuts at: 716 1684 2369 716
Size: 968 685 2984
Fragments arranged by size:
2984 968 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4637
PvuII CAG'CTG
Cuts at: 1122 2062 2422 1122
Size: 940 360 3337
Fragments arranged by size:
3337 940 360
RsrII CG'GwC_CG
Cuts at: 2832 2832
Size: 4637
SacI G_AGCT'C
Cuts at: 1651 1651
Size: 4637
SacII CC_GC'GG
Cuts at: 134 323 134
Size: 189 4448
SalI G'TCGA_C
Cuts at: 1674 1674
Size: 4637
SanDI GG'GwC_CC
Cuts at: 237 237
Size: 4637
SapI GCTCTTCn'nnn_
Cuts at: 2660 2870 2660
Size: 210 4427
SbfI CC_TGCA'GG
Cuts at: 1684 1684
Size: 4637
ScaI AGT'ACT
Cuts at: 4154 4154
Size: 4637
SexAI A'CCwGG_T
Cuts at: 851 851
Size: 4637
SfcI C'TryA_G
Cuts at: 33 333 712 1588 1680 2365 3358 3549 33
Size: 300 379 876 92 685 993 191 1121
Fragments arranged by size:
1121 993 876 685 379 300 191 92
SfoI GGC'GCC
Cuts at: 314 384 2069 2317 314
Size: 70 1685 248 2634
Fragments arranged by size:
2634 1685 248 70
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4637
SmaI CCC'GGG
Cuts at: 243 529 1659 243
Size: 286 1130 3221
Fragments arranged by size:
3221 1130 286
SmlI C'TyrA_G
Cuts at: 1792 2707 3199 3461 1792
Size: 915 492 262 2968
Fragments arranged by size:
2968 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4637
SphI G_CATG'C
Cuts at: 1348 1690 2190 1348
Size: 342 500 3795
Fragments arranged by size:
3795 500 342
StuI AGG'CCT
Cuts at: 622 622
Size: 4637
StyI C'CwwG_G
Cuts at: 884 1771 2125 884
Size: 887 354 3396
Fragments arranged by size:
3396 887 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1306 1366 2220 2353 2812 3021 1306
Size: 60 854 133 459 209 2922
Fragments arranged by size:
2922 854 459 209 133 60
TatI w'GTAC_w
Cuts at: 1081 4152 1081
Size: 3071 1566
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4154 4186 4154
Size: 32 4605
Tth111I GACn'n_nGTC
Cuts at: 2434 2434
Size: 4637
XbaI T'CTAG_A
Cuts at: 1668 1668
Size: 4637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1371 1459 2132 1371
Size: 88 673 3876
Fragments arranged by size:
3876 673 88
XmaI C'CCGG_G
Cuts at: 241 527 1657 241
Size: 286 1130 3221
Fragments arranged by size:
3221 1130 286
XmnI GAAnn'nnTTC
Cuts at: 1405 1405
Size: 4637
ZraI GAC'GTC
Cuts at: 1295 1295
Size: 4637
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsrGI BssHII
BstEII Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI