Restriction map for
pF1KB7782
(Circular) (Six-base) MAPSORT of: pF1KB7782.seq Check: 923 from: 1 to: 4643
pF1KB7782 4643 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1148 1148
Size: 4643
AccI GT'mk_AC
Cuts at: 1681 1681
Size: 4643
Acc65I G'GTAC_C
Cuts at: 1659 1659
Size: 4643
AclI AA'CG_TT
Cuts at: 4483 4483
Size: 4643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 229 565 568 991 2468 2900 3647 3915
Size: 78 336 3 423 1477 432 747 268
Cuts at: 3915 151
Size: 879
Fragments arranged by size:
1477 879 747 432 423 336 268 78
3
AflIII A'CryG_T
Cuts at: 1995 3099 4076 1995
Size: 1104 977 2562
Fragments arranged by size:
2562 1104 977
AgeI A'CCGG_T
Cuts at: 1060 3031 1060
Size: 1971 2672
AleI CACnn'nnGTG
Cuts at: 1063 1063
Size: 4643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1146 1146
Size: 4643
AlwNI CAG_nnn'CTG
Cuts at: 767 969 1948 3515 767
Size: 202 979 1567 1895
Fragments arranged by size:
1895 1567 979 202
ApaI G_GGCC'C
Cuts at: 234 1384 1589 234
Size: 1150 205 3288
Fragments arranged by size:
3288 1150 205
ApaLI G'TGCA_C
Cuts at: 3413 3413
Size: 4643
ApoI r'AATT_y
Cuts at: 1647 3996 4252 1647
Size: 2349 256 2038
Fragments arranged by size:
2349 2038 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4643
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
AvaI C'yCGr_G
Cuts at: 777 802 1663 777
Size: 25 861 3757
Fragments arranged by size:
3757 861 25
BamHI G'GATC_C
Cuts at: 1668 1668
Size: 4643
BanI G'GyrC_C
Cuts at: 1319 1363 1432 1492 1626 1659 2073 2321 2356
Size: 44 69 60 134 33 414 248 35
Cuts at: 2356 1319
Size: 3606
Fragments arranged by size:
3606 414 248 134 69 60 44 35
33
BanII G_rGCy'C
Cuts at: 221 234 595 815 1253 1384 1403 1589 1657
Size: 13 361 220 438 131 19 186 68
Cuts at: 1657 2687 221
Size: 1030 2177
Fragments arranged by size:
2177 1030 438 361 220 186 131 68
19 13
BbeI G_GCGC'C
Cuts at: 1367 1496 2077 2325 1367
Size: 129 581 248 3685
Fragments arranged by size:
3685 581 248 129
BbsI GAAGACnn'nnnn_
Cuts at: 979 1152 1574 979
Size: 173 422 4048
Fragments arranged by size:
4048 422 173
BciVI GTATCCnnnnn_n'
Cuts at: 2541 3302 4643 2541
Size: 761 1341 2541
Fragments arranged by size:
2541 1341 761
BclI T'GATC_A
Cuts at: 2163 2163
Size: 4643
BglI GCCn_nnn'nGGC
Cuts at: 112 342 819 1361 1466 112
Size: 230 477 542 105 3289
Fragments arranged by size:
3289 542 477 230 105
BglII A'GATC_T
Cuts at: 2158 4195 2158
Size: 2037 2606
BlpI GC'TnA_GC
Cuts at: 1755 1755
Size: 4643
Bme1580I G_kGCm'C
Cuts at: 234 398 1115 1204 1324 1384 1437 1589 2268
Size: 164 717 89 120 60 53 152 679
Cuts at: 2268 2361 3417 234
Size: 93 1056 1460
Fragments arranged by size:
1460 1056 717 679 164 152 120 93
89 60 53
BmrI ACTGGGnnnn_n'
Cuts at: 472 1855 1936 2271 4415 472
Size: 1383 81 335 2144 700
Fragments arranged by size:
2144 1383 700 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 4643
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 627 220
Size: 407 4236
Bpu10I CC'TnA_GC
Cuts at: 263 1267 263
Size: 1004 3639
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1819 2698 3190 3488 472
Size: 1347 879 492 298 1627
Fragments arranged by size:
1627 1347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 289 1120 4347 289
Size: 831 3227 585
Fragments arranged by size:
3227 831 585
BsaAI yAC'GTr
Cuts at: 1996 1996
Size: 4643
BsaBI GATnn'nnATC
Cuts at: 6 2157 4194 6
Size: 2151 2037 455
Fragments arranged by size:
2151 2037 455
BsaHI Gr'CG_yC
Cuts at: 613 1154 1283 1364 1391 1493 2074 2322 3024
Size: 541 129 81 27 102 581 248 702
Cuts at: 3024 4520 613
Size: 1496 736
Fragments arranged by size:
1496 736 702 581 541 248 129 102
81 27
BsaWI w'CCGG_w
Cuts at: 635 1060 1832 2353 3031 3305 3452 3863 635
Size: 425 772 521 678 274 147 411 1415
Fragments arranged by size:
1415 772 678 521 425 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 186 216 580 610 712 742 775 805 186
Size: 30 364 30 102 30 33 30 4024
Fragments arranged by size:
4024 364 102 33 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1243 1558 1561 1564 1573 1576 1243
Size: 315 3 3 9 3 4310
Fragments arranged by size:
4310 315 9 3 3 3
BseYI C'CCAG_C
Cuts at: 1377 1414 2068 2826 3403 3899 1377
Size: 37 654 758 577 496 2121
Fragments arranged by size:
2121 758 654 577 496 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 694 265
Size: 429 4214
BsiEI CG_ry'CG
Cuts at: 104 381 2185 2231 3439 104
Size: 277 1804 46 1208 1308
Fragments arranged by size:
1804 1308 1208 277 46
BsiHKAI G_wGCw'C
Cuts at: 221 440 1657 2435 2625 3417 221
Size: 219 1217 778 190 792 1447
Fragments arranged by size:
1447 1217 792 778 219 190
Bsp1286I G_dGCh'C
Cuts at: 221 234 398 440 595 815 1115 1204 1253
Size: 13 164 42 155 220 300 89 49
Cuts at: 1253 1324 1384 1403 1437 1589 1657 2268 2361
Size: 71 60 19 34 152 68 611 93
Cuts at: 2361 2435 2625 2687 3417 221
Size: 74 190 62 730 1447
Fragments arranged by size:
1447 730 611 300 220 190 164 155
152 93 89 74 71 68 62 60
49 42 34 19 13
BspEI T'CCGG_A
Cuts at: 635 3863 635
Size: 3228 1415
BspHI T'CATG_A
Cuts at: 3819 3819
Size: 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 666 1138 1148 1175 1693 1907 2209 2590 666
Size: 472 10 27 518 214 302 381 2719
Fragments arranged by size:
2719 518 472 381 302 214 27 10
BsrBI CCG'CTC
Cuts at: 50 1430 2935 2989 4473 4638 50
Size: 1380 1505 54 1484 165 55
Fragments arranged by size:
1505 1484 1380 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2555 2555
Size: 4643
BsrFI r'CCGG_y
Cuts at: 341 385 1060 1629 2641 2822 3031 341
Size: 44 675 569 1012 181 209 1953
Fragments arranged by size:
1953 1012 675 569 209 181 44
BssSI C'ACGA_G
Cuts at: 214 1314 1372 2914 3272 214
Size: 1100 58 1542 358 1585
Fragments arranged by size:
1585 1542 1100 358 58
BstAPI GCAn_nnn'nTGC
Cuts at: 2020 2020
Size: 4643
BstBI TT'CG_AA
Cuts at: 3004 3004
Size: 4643
BstXI CCAn_nnnn'nTGG
Cuts at: 138 138
Size: 4643
BstYI r'GATC_y
Cuts at: 1 1262 1668 2135 2158 2493 2739 3751 3837
Size: 1261 406 467 23 335 246 1012 86
Cuts at: 3837 3849 4195 1
Size: 12 346 449
Fragments arranged by size:
1261 1012 467 449 406 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 716 716
Size: 4643
BtgI C'CryG_G
Cuts at: 107 107
Size: 4643
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 726 2593 2774 2937 726
Size: 1867 181 163 2432
Fragments arranged by size:
2432 1867 181 163
BtsI GCAGTG_nn'
Cuts at: 1150 1886 1150
Size: 736 3907
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 605 640 605
Size: 35 4608
DraI TTT'AAA
Cuts at: 1642 1642
Size: 4643
DrdI GACnn_nn'nnGTC
Cuts at: 2349 3207 2349
Size: 858 3785
EaeI y'GGCC_r
Cuts at: 866 2228 2402 2793 2820 4165 866
Size: 1362 174 391 27 1345 1344
Fragments arranged by size:
1362 1345 1344 391 174 27
EagI C'GGCC_G
Cuts at: 2228 2228
Size: 4643
EarI CTCTTCn'nnn_
Cuts at: 1550 1574 2666 2876 1550
Size: 24 1092 210 3317
Fragments arranged by size:
3317 1092 210 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 490 2851 3161 3307 4453 130
Size: 360 2361 310 146 1146 320
Fragments arranged by size:
2361 1146 360 320 310 146
EcoICRI GAG'CTC
Cuts at: 219 1655 219
Size: 1436 3207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 220 229 565 568 627 991 2468 2900
Size: 69 9 336 3 59 364 1477 432
Cuts at: 2900 3647 3915 151
Size: 747 268 879
Fragments arranged by size:
1477 879 747 432 364 336 268 69
59 9 3
EcoO109I rG'GnC_Cy
Cuts at: 230 231 415 1381 1585 1586 1620 1782 230
Size: 1 184 966 204 1 34 162 3091
Fragments arranged by size:
3091 966 204 184 162 34 1 1
EcoRI G'AATT_C
Cuts at: 1647 1647
Size: 4643
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4643
FspI TGC'GCA
Cuts at: 2424 2424
Size: 4643
HaeII r_GCGC'y
Cuts at: 392 1367 1496 1602 2077 2325 3347 4326 392
Size: 975 129 106 475 248 1022 979 709
Fragments arranged by size:
1022 979 975 709 475 248 129 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1510 1542 2485 2515 2517 2547 1510
Size: 32 943 30 2 30 3606
Fragments arranged by size:
3606 943 32 30 30 2
HincII GTy'rAC
Cuts at: 756 1422 1682 756
Size: 666 260 3717
Fragments arranged by size:
3717 666 260
HindIII A'AGCT_T
Cuts at: 580 580
Size: 4643
KasI G'GCGC_C
Cuts at: 1363 1492 2073 2321 1363
Size: 129 581 248 3685
Fragments arranged by size:
3685 581 248 129
KpnI G_GTAC'C
Cuts at: 1663 1663
Size: 4643
MluI A'CGCG_T
Cuts at: 4076 4076
Size: 4643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 3314 3498 185
Size: 3129 184 1330
Fragments arranged by size:
3129 1330 184
MscI TGG'CCA
Cuts at: 2404 2404
Size: 4643
MslI CAynn'nnrTG
Cuts at: 142 745 1063 1195 1372 2632 2759 142
Size: 603 318 132 177 1260 127 2026
Fragments arranged by size:
2026 1260 603 318 177 132 127
MspA1I CmG'CkG
Cuts at: 240 408 710 942 1089 1349 1604 1754 2068
Size: 168 302 232 147 260 255 150 314
Cuts at: 2068 2428 3441 3686 240
Size: 360 1013 245 1197
Fragments arranged by size:
1197 1013 360 314 302 260 255 245
232 168 150 147
NaeI GCC'GGC
Cuts at: 387 1631 2824 387
Size: 1244 1193 2206
Fragments arranged by size:
2206 1244 1193
NarI GG'CG_CC
Cuts at: 1364 1493 2074 2322 1364
Size: 129 581 248 3685
Fragments arranged by size:
3685 581 248 129
NcoI C'CATG_G
Cuts at: 107 107
Size: 4643
NgoMIV G'CCGG_C
Cuts at: 385 1629 2822 385
Size: 1244 1193 2206
Fragments arranged by size:
2206 1244 1193
NspI r_CATG'y
Cuts at: 1696 2196 3103 1696
Size: 500 907 3236
Fragments arranged by size:
3236 907 500
PasI CC'CwG_GG
Cuts at: 1437 1437
Size: 4643
PciI A'CATG_T
Cuts at: 3099 3099
Size: 4643
PmeI GTTT'AAAC
Cuts at: 1642 1642
Size: 4643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 4611
PspOMI G'GGCC_C
Cuts at: 230 1380 1585 230
Size: 1150 205 3288
Fragments arranged by size:
3288 1150 205
PstI C_TGCA'G
Cuts at: 656 965 1145 1172 1354 1690 2375 656
Size: 309 180 27 182 336 685 2924
Fragments arranged by size:
2924 685 336 309 182 180 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4643
PvuII CAG'CTG
Cuts at: 240 710 942 1349 2068 2428 240
Size: 470 232 407 719 360 2455
Fragments arranged by size:
2455 719 470 407 360 232
RsrII CG'GwC_CG
Cuts at: 2838 2838
Size: 4643
SacI G_AGCT'C
Cuts at: 221 1657 221
Size: 1436 3207
SalI G'TCGA_C
Cuts at: 1680 1680
Size: 4643
SapI GCTCTTCn'nnn_
Cuts at: 2666 2876 2666
Size: 210 4433
SbfI CC_TGCA'GG
Cuts at: 1690 1690
Size: 4643
ScaI AGT'ACT
Cuts at: 451 4160 451
Size: 3709 934
SexAI A'CCwGG_T
Cuts at: 929 1301 929
Size: 372 4271
SfcI C'TryA_G
Cuts at: 33 255 652 961 1141 1168 1350 1686 2371
Size: 222 397 309 180 27 182 336 685
Cuts at: 2371 3364 3555 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 397 336 309 222 191
182 180 27
SfoI GGC'GCC
Cuts at: 1365 1494 2075 2323 1365
Size: 129 581 248 3685
Fragments arranged by size:
3685 581 248 129
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
SgrAI Cr'CCGG_yG
Cuts at: 1060 1060
Size: 4643
SmaI CCC'GGG
Cuts at: 779 804 1665 779
Size: 25 861 3757
Fragments arranged by size:
3757 861 25
SmlI C'TyrA_G
Cuts at: 487 1798 2713 3205 3467 487
Size: 1311 915 492 262 1663
Fragments arranged by size:
1663 1311 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4643
SphI G_CATG'C
Cuts at: 1696 2196 1696
Size: 500 4143
SspI AAT'ATT
Cuts at: 423 423
Size: 4643
StyI C'CwwG_G
Cuts at: 107 1777 2131 107
Size: 1670 354 2619
Fragments arranged by size:
2619 1670 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 859 2226 2359 2818 3027 859
Size: 1367 133 459 209 2475
Fragments arranged by size:
2475 1367 459 209 133
TatI w'GTAC_w
Cuts at: 449 4158 449
Size: 3709 934
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 186 218 580 612 1300 1332 4160 4192 186
Size: 32 362 32 688 32 2828 32 637
Fragments arranged by size:
2828 688 637 362 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1156 2440 1156
Size: 1284 3359
XbaI T'CTAG_A
Cuts at: 1674 1674
Size: 4643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 405 1374 2138 405
Size: 969 764 2910
Fragments arranged by size:
2910 969 764
XmaI C'CCGG_G
Cuts at: 777 802 1663 777
Size: 25 861 3757
Fragments arranged by size:
3757 861 25
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI