Restriction map for
pF1KB7786
(Circular) (Six-base) MAPSORT of: pF1KB7786.seq Check: 2837 from: 1 to: 4649
pF1KB7786 4649 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1687 1687
Size: 4649
Acc65I G'GTAC_C
Cuts at: 1665 1665
Size: 4649
AclI AA'CG_TT
Cuts at: 4489 4489
Size: 4649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1472 1580 2474 2906 3653 3921 1472
Size: 108 894 432 747 268 2200
Fragments arranged by size:
2200 894 747 432 268 108
AflIII A'CryG_T
Cuts at: 2001 3105 4082 2001
Size: 1104 977 2568
Fragments arranged by size:
2568 1104 977
AgeI A'CCGG_T
Cuts at: 3037 3037
Size: 4649
AleI CACnn'nnGTG
Cuts at: 1098 1098
Size: 4649
AlwNI CAG_nnn'CTG
Cuts at: 706 974 1633 1636 1954 3521 706
Size: 268 659 3 318 1567 1834
Fragments arranged by size:
1834 1567 659 318 268 3
ApaI G_GGCC'C
Cuts at: 387 387
Size: 4649
ApaLI G'TGCA_C
Cuts at: 524 1487 3419 524
Size: 963 1932 1754
Fragments arranged by size:
1932 1754 963
ApoI r'AATT_y
Cuts at: 295 448 1653 4002 4258 295
Size: 153 1205 2349 256 686
Fragments arranged by size:
2349 1205 686 256 153
AseI AT'TA_AT
Cuts at: 20 20
Size: 4649
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
AvaI C'yCGr_G
Cuts at: 1565 1669 1565
Size: 104 4545
BamHI G'GATC_C
Cuts at: 309 410 726 1272 1674 309
Size: 101 316 546 402 3284
Fragments arranged by size:
3284 546 402 316 101
BanI G'GyrC_C
Cuts at: 713 1366 1665 2079 2327 2362 713
Size: 653 299 414 248 35 3000
Fragments arranged by size:
3000 653 414 299 248 35
BanII G_rGCy'C
Cuts at: 387 958 1082 1484 1663 2693 387
Size: 571 124 402 179 1030 2343
Fragments arranged by size:
2343 1030 571 402 179 124
BbeI G_GCGC'C
Cuts at: 717 2083 2331 717
Size: 1366 248 3035
Fragments arranged by size:
3035 1366 248
BbsI GAAGACnn'nnnn_
Cuts at: 1460 1460
Size: 4649
BciVI GTATCCnnnnn_n'
Cuts at: 2547 3308 4649 2547
Size: 761 1341 2547
Fragments arranged by size:
2547 1341 761
BclI T'GATC_A
Cuts at: 2169 2169
Size: 4649
BfrBI ATG'CAT
Cuts at: 1110 1110
Size: 4649
BglI GCCn_nnn'nGGC
Cuts at: 335 335
Size: 4649
BglII A'GATC_T
Cuts at: 2164 4201 2164
Size: 2037 2612
BlpI GC'TnA_GC
Cuts at: 376 699 1573 1761 376
Size: 323 874 188 3264
Fragments arranged by size:
3264 874 323 188
Bme1580I G_kGCm'C
Cuts at: 387 528 1333 1415 1491 2274 2367 3423 387
Size: 141 805 82 76 783 93 1056 1613
Fragments arranged by size:
1613 1056 805 783 141 93 82 76
BmrI ACTGGGnnnn_n'
Cuts at: 180 1478 1861 1942 2277 4421 180
Size: 1298 383 81 335 2144 408
Fragments arranged by size:
2144 1298 408 383 335 81
BmtI G_CTAG'C
Cuts at: 623 623
Size: 4649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 714 3016 714
Size: 2302 2347
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 709 723 1462 253
Size: 456 14 739 3440
Fragments arranged by size:
3440 739 456 14
Bpu10I CC'TnA_GC
Cuts at: 952 952
Size: 4649
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1304 1825 2704 3196 3494 1004
Size: 300 521 879 492 298 2159
Fragments arranged by size:
2159 879 521 492 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 4353 4353
Size: 4649
BsaAI yAC'GTr
Cuts at: 2002 2002
Size: 4649
BsaBI GATnn'nnATC
Cuts at: 6 2163 4200 6
Size: 2157 2037 455
Fragments arranged by size:
2157 2037 455
BsaHI Gr'CG_yC
Cuts at: 714 2080 2328 3030 4526 714
Size: 1366 248 702 1496 837
Fragments arranged by size:
1496 1366 837 702 248
BsaWI w'CCGG_w
Cuts at: 1838 2359 3037 3311 3458 3869 1838
Size: 521 678 274 147 411 2618
Fragments arranged by size:
2618 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 567 597 567
Size: 30 4619
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 773 1132 1210 1324 773
Size: 359 78 114 4098
Fragments arranged by size:
4098 359 114 78
BseYI C'CCAG_C
Cuts at: 2074 2832 3409 3905 2074
Size: 758 577 496 2818
Fragments arranged by size:
2818 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1373 619
Size: 754 3895
BsiEI CG_ry'CG
Cuts at: 104 2191 2237 3445 104
Size: 2087 46 1208 1308
Fragments arranged by size:
2087 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 528 1316 1491 1663 2441 2631 3423 528
Size: 788 175 172 778 190 792 1754
Fragments arranged by size:
1754 792 788 778 190 175 172
BsmI GAATG_Cn'
Cuts at: 750 750
Size: 4649
Bsp1286I G_dGCh'C
Cuts at: 387 528 958 1082 1316 1333 1415 1484 1491
Size: 141 430 124 234 17 82 69 7
Cuts at: 1491 1663 2274 2367 2441 2631 2693 3423 387
Size: 172 611 93 74 190 62 730 1613
Fragments arranged by size:
1613 730 611 430 234 190 172 141
124 93 82 74 69 62 17 7
BspEI T'CCGG_A
Cuts at: 3869 3869
Size: 4649
BspHI T'CATG_A
Cuts at: 3825 3825
Size: 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1266 1699 1913 2215 2596 1266
Size: 433 214 302 381 3319
Fragments arranged by size:
3319 433 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2941 2995 4479 4644 50
Size: 2891 54 1484 165 55
Fragments arranged by size:
2891 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2561 2561
Size: 4649
BsrFI r'CCGG_y
Cuts at: 115 2647 2828 3037 115
Size: 2532 181 209 1727
Fragments arranged by size:
2532 1727 209 181
BssSI C'ACGA_G
Cuts at: 1298 2920 3278 1298
Size: 1622 358 2669
Fragments arranged by size:
2669 1622 358
BstAPI GCAn_nnn'nTGC
Cuts at: 347 974 2026 347
Size: 627 1052 2970
Fragments arranged by size:
2970 1052 627
BstBI TT'CG_AA
Cuts at: 3010 3010
Size: 4649
BstXI CCAn_nnnn'nTGG
Cuts at: 1400 1400
Size: 4649
BstYI r'GATC_y
Cuts at: 1 209 309 410 726 1272 1674 2141 2164
Size: 208 100 101 316 546 402 467 23
Cuts at: 2164 2499 2745 3757 3843 3855 4201 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 546 467 449 402 346 335 316
246 208 101 100 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 992 1115 1285 992
Size: 123 170 4356
Fragments arranged by size:
4356 170 123
BtgI C'CryG_G
Cuts at: 870 1223 870
Size: 353 4296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 252 2599 2780 2943 252
Size: 2347 181 163 1958
Fragments arranged by size:
2347 1958 181 163
BtsI GCAGTG_nn'
Cuts at: 1093 1105 1892 1093
Size: 12 787 3850
Fragments arranged by size:
3850 787 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 491 526 491
Size: 35 4614
DraI TTT'AAA
Cuts at: 1648 1648
Size: 4649
DrdI GACnn_nn'nnGTC
Cuts at: 2355 3213 2355
Size: 858 3791
EaeI y'GGCC_r
Cuts at: 639 768 867 2234 2408 2799 2826 4171 639
Size: 129 99 1367 174 391 27 1345 1117
Fragments arranged by size:
1367 1345 1117 391 174 129 99 27
EagI C'GGCC_G
Cuts at: 2234 2234
Size: 4649
EarI CTCTTCn'nnn_
Cuts at: 245 552 1319 1349 2672 2882 245
Size: 307 767 30 1323 210 2012
Fragments arranged by size:
2012 1323 767 307 210 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 247 1179 2857 3167 3313 4459 247
Size: 932 1678 310 146 1146 437
Fragments arranged by size:
1678 1146 932 437 310 146
EcoICRI GAG'CTC
Cuts at: 1661 1661
Size: 4649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 709 723 1462 1472 1580 2474 2906 3653
Size: 456 14 739 10 108 894 432 747
Cuts at: 3653 3921 253
Size: 268 981
Fragments arranged by size:
981 894 747 739 456 432 268 108
14 10
EcoO109I rG'GnC_Cy
Cuts at: 384 1609 1788 384
Size: 1225 179 3245
Fragments arranged by size:
3245 1225 179
EcoRI G'AATT_C
Cuts at: 1653 1653
Size: 4649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2668 2668
Size: 4649
FspI TGC'GCA
Cuts at: 2430 2430
Size: 4649
HaeII r_GCGC'y
Cuts at: 717 1545 2083 2331 3353 4332 717
Size: 828 538 248 1022 979 1034
Fragments arranged by size:
1034 1022 979 828 538 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 718 750 756 788 2491 2521 2523 2553 718
Size: 32 6 32 1703 30 2 30 2814
Fragments arranged by size:
2814 1703 32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 1124 1688 1124
Size: 564 4085
HindIII A'AGCT_T
Cuts at: 1022 1525 1022
Size: 503 4146
KasI G'GCGC_C
Cuts at: 713 2079 2327 713
Size: 1366 248 3035
Fragments arranged by size:
3035 1366 248
KpnI G_GTAC'C
Cuts at: 1669 1669
Size: 4649
MluI A'CGCG_T
Cuts at: 4082 4082
Size: 4649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 610 3320 3504 610
Size: 2710 184 1755
Fragments arranged by size:
2710 1755 184
MscI TGG'CCA
Cuts at: 641 770 2410 641
Size: 129 1640 2880
Fragments arranged by size:
2880 1640 129
MslI CAynn'nnrTG
Cuts at: 1098 2638 2765 1098
Size: 1540 127 2982
Fragments arranged by size:
2982 1540 127
MspA1I CmG'CkG
Cuts at: 216 703 872 974 1633 1760 2074 2434 3447
Size: 487 169 102 659 127 314 360 1013
Cuts at: 3447 3692 216
Size: 245 1173
Fragments arranged by size:
1173 1013 659 487 360 314 245 169
127 102
NaeI GCC'GGC
Cuts at: 117 2830 117
Size: 2713 1936
NarI GG'CG_CC
Cuts at: 714 2080 2328 714
Size: 1366 248 3035
Fragments arranged by size:
3035 1366 248
NcoI C'CATG_G
Cuts at: 1223 1223
Size: 4649
NgoMIV G'CCGG_C
Cuts at: 115 2828 115
Size: 2713 1936
NheI G'CTAG_C
Cuts at: 619 619
Size: 4649
NsiI A_TGCA'T
Cuts at: 1112 1112
Size: 4649
NspI r_CATG'y
Cuts at: 1702 2202 3109 1702
Size: 500 907 3242
Fragments arranged by size:
3242 907 500
PasI CC'CwG_GG
Cuts at: 1075 1614 1075
Size: 539 4110
PciI A'CATG_T
Cuts at: 3105 3105
Size: 4649
PflMI CCAn_nnn'nTGG
Cuts at: 865 1159 1620 865
Size: 294 461 3894
Fragments arranged by size:
3894 461 294
PfoI T'CCnGG_A
Cuts at: 230 739 230
Size: 509 4140
PmeI GTTT'AAAC
Cuts at: 1648 1648
Size: 4649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4617
PpuMI rG'GwC_Cy
Cuts at: 1609 1609
Size: 4649
PspOMI G'GGCC_C
Cuts at: 383 383
Size: 4649
PstI C_TGCA'G
Cuts at: 245 1100 1148 1696 2381 245
Size: 855 48 548 685 2513
Fragments arranged by size:
2513 855 685 548 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4649
PvuII CAG'CTG
Cuts at: 216 703 974 1633 2074 2434 216
Size: 487 271 659 441 360 2431
Fragments arranged by size:
2431 659 487 441 360 271
RsrII CG'GwC_CG
Cuts at: 2844 2844
Size: 4649
SacI G_AGCT'C
Cuts at: 1663 1663
Size: 4649
SacII CC_GC'GG
Cuts at: 873 873
Size: 4649
SalI G'TCGA_C
Cuts at: 1686 1686
Size: 4649
SapI GCTCTTCn'nnn_
Cuts at: 2672 2882 2672
Size: 210 4439
SbfI CC_TGCA'GG
Cuts at: 1696 1696
Size: 4649
ScaI AGT'ACT
Cuts at: 4166 4166
Size: 4649
SfcI C'TryA_G
Cuts at: 33 120 241 933 1096 1144 1692 2377 3370
Size: 87 121 692 163 48 548 685 993
Cuts at: 3370 3561 33
Size: 191 1121
Fragments arranged by size:
1121 993 692 685 548 191 163 121
87 48
SfoI GGC'GCC
Cuts at: 715 2081 2329 715
Size: 1366 248 3035
Fragments arranged by size:
3035 1366 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
SmaI CCC'GGG
Cuts at: 1671 1671
Size: 4649
SmlI C'TyrA_G
Cuts at: 1019 1319 1804 2719 3211 3473 1019
Size: 300 485 915 492 262 2195
Fragments arranged by size:
2195 915 492 485 300 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4649
SphI G_CATG'C
Cuts at: 1702 2202 1702
Size: 500 4149
StuI AGG'CCT
Cuts at: 950 1470 950
Size: 520 4129
StyI C'CwwG_G
Cuts at: 1223 1783 2137 1223
Size: 560 354 3735
Fragments arranged by size:
3735 560 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 488 592 1410 2232 2365 2824 3033 488
Size: 104 818 822 133 459 209 2104
Fragments arranged by size:
2104 822 818 459 209 133 104
TatI w'GTAC_w
Cuts at: 4164 4164
Size: 4649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 567 599 4166 4198 567
Size: 32 3567 32 1018
Fragments arranged by size:
3567 1018 32 32
Tth111I GACn'n_nGTC
Cuts at: 2446 2446
Size: 4649
XbaI T'CTAG_A
Cuts at: 1680 1680
Size: 4649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1221 2144 1221
Size: 923 3726
XmaI C'CCGG_G
Cuts at: 1669 1669
Size: 4649
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BmgBI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NotI NruI PacI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI