Restriction map for
pF1KB7791
(Circular) (Six-base) MAPSORT of: pF1KB7791.seq Check: 6613 from: 1 to: 4490
pF1KB7791 4490 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 691 691
Size: 4490
AccI GT'mk_AC
Cuts at: 1528 1528
Size: 4490
Acc65I G'GTAC_C
Cuts at: 1506 1506
Size: 4490
AclI AA'CG_TT
Cuts at: 4330 4330
Size: 4490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 1236 2315 2747 3494 3762 279
Size: 957 1079 432 747 268 1007
Fragments arranged by size:
1079 1007 957 747 432 268
AfeI AGC'GCT
Cuts at: 247 1081 247
Size: 834 3656
AflIII A'CryG_T
Cuts at: 1842 2946 3923 1842
Size: 1104 977 2409
Fragments arranged by size:
2409 1104 977
AgeI A'CCGG_T
Cuts at: 1378 2878 1378
Size: 1500 2990
AleI CACnn'nnGTG
Cuts at: 1143 1379 1143
Size: 236 4254
AlwNI CAG_nnn'CTG
Cuts at: 360 387 1322 1455 1795 3362 360
Size: 27 935 133 340 1567 1488
Fragments arranged by size:
1567 1488 935 340 133 27
ApaI G_GGCC'C
Cuts at: 982 982
Size: 4490
ApaLI G'TGCA_C
Cuts at: 1204 1372 3260 1204
Size: 168 1888 2434
Fragments arranged by size:
2434 1888 168
ApoI r'AATT_y
Cuts at: 1494 3843 4099 1494
Size: 2349 256 1885
Fragments arranged by size:
2349 1885 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4490
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
AvaI C'yCGr_G
Cuts at: 334 435 532 743 928 1510 334
Size: 101 97 211 185 582 3314
Fragments arranged by size:
3314 582 211 185 101 97
BamHI G'GATC_C
Cuts at: 550 1515 550
Size: 965 3525
BanI G'GyrC_C
Cuts at: 757 876 885 1022 1109 1506 1920 2168 2203
Size: 119 9 137 87 397 414 248 35
Cuts at: 2203 757
Size: 3044
Fragments arranged by size:
3044 414 397 248 137 119 87 35
9
BanII G_rGCy'C
Cuts at: 314 839 928 982 1096 1504 2534 314
Size: 525 89 54 114 408 1030 2270
Fragments arranged by size:
2270 1030 525 408 114 89 54
BbeI G_GCGC'C
Cuts at: 1026 1924 2172 1026
Size: 898 248 3344
Fragments arranged by size:
3344 898 248
BbvCI CC'TCA_GC
Cuts at: 938 938
Size: 4490
BciVI GTATCCnnnnn_n'
Cuts at: 2388 3149 4490 2388
Size: 761 1341 2388
Fragments arranged by size:
2388 1341 761
BclI T'GATC_A
Cuts at: 2010 2010
Size: 4490
BglI GCCn_nnn'nGGC
Cuts at: 899 1199 1270 1468 899
Size: 300 71 198 3921
Fragments arranged by size:
3921 300 198 71
BglII A'GATC_T
Cuts at: 2005 4042 2005
Size: 2037 2453
BlpI GC'TnA_GC
Cuts at: 773 1602 773
Size: 829 3661
Bme1580I G_kGCm'C
Cuts at: 515 588 760 982 1150 1208 1376 2115 2208
Size: 73 172 222 168 58 168 739 93
Cuts at: 2208 3264 515
Size: 1056 1741
Fragments arranged by size:
1741 1056 739 222 172 168 168 93
73 58
BmgBI CAC'GTC
Cuts at: 909 909
Size: 4490
BmrI ACTGGGnnnn_n'
Cuts at: 976 1702 1783 2118 4262 976
Size: 726 81 335 2144 1204
Fragments arranged by size:
2144 1204 726 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2857 2857
Size: 4490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 397 942 382
Size: 15 545 3930
Fragments arranged by size:
3930 545 15
Bpu10I CC'TnA_GC
Cuts at: 938 938
Size: 4490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1666 2545 3037 3335 1666
Size: 879 492 298 2821
Fragments arranged by size:
2821 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4194 4194
Size: 4490
BsaAI yAC'GTr
Cuts at: 1843 1843
Size: 4490
BsaBI GATnn'nnATC
Cuts at: 6 2004 4041 6
Size: 1998 2037 455
Fragments arranged by size:
2037 1998 455
BsaHI Gr'CG_yC
Cuts at: 1023 1921 2169 2871 4367 1023
Size: 898 248 702 1496 1146
Fragments arranged by size:
1496 1146 898 702 248
BsaWI w'CCGG_w
Cuts at: 1378 1679 2200 2878 3152 3299 3710 1378
Size: 301 521 678 274 147 411 2158
Fragments arranged by size:
2158 678 521 411 301 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 552 582 552
Size: 30 4460
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 463 535 946 1167 463
Size: 72 411 221 3786
Fragments arranged by size:
3786 411 221 72
BseYI C'CCAG_C
Cuts at: 206 416 632 1088 1472 1915 2673 3250 3746
Size: 210 216 456 384 443 758 577 496
Cuts at: 3746 206
Size: 950
Fragments arranged by size:
950 758 577 496 456 443 384 216
210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1441 1441
Size: 4490
BsiEI CG_ry'CG
Cuts at: 104 598 964 2032 2078 3286 104
Size: 494 366 1068 46 1208 1308
Fragments arranged by size:
1308 1208 1068 494 366 46
BsiHKAI G_wGCw'C
Cuts at: 314 1208 1376 1504 2282 2472 3264 314
Size: 894 168 128 778 190 792 1540
Fragments arranged by size:
1540 894 792 778 190 168 128
BsmBI CGTCTCn'nnnn_
Cuts at: 664 664
Size: 4490
Bsp1286I G_dGCh'C
Cuts at: 314 515 588 760 839 928 982 1096 1150
Size: 201 73 172 79 89 54 114 54
Cuts at: 1150 1208 1376 1504 2115 2208 2282 2472 2534
Size: 58 168 128 611 93 74 190 62
Cuts at: 2534 3264 314
Size: 730 1540
Fragments arranged by size:
1540 730 611 201 190 172 168 128
114 93 89 79 74 73 62 58
54 54
BspEI T'CCGG_A
Cuts at: 3710 3710
Size: 4490
BspHI T'CATG_A
Cuts at: 285 3666 285
Size: 3381 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 691 1361 1540 1754 2056 2437 691
Size: 670 179 214 302 381 2744
Fragments arranged by size:
2744 670 381 302 214 179
BsrBI CCG'CTC
Cuts at: 50 1386 2782 2836 4320 4485 50
Size: 1336 1396 54 1484 165 55
Fragments arranged by size:
1484 1396 1336 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2402 2402
Size: 4490
BsrFI r'CCGG_y
Cuts at: 879 959 1019 1378 2488 2669 2878 879
Size: 80 60 359 1110 181 209 2491
Fragments arranged by size:
2491 1110 359 209 181 80 60
BssHII G'CGCG_C
Cuts at: 992 1428 1430 992
Size: 436 2 4052
Fragments arranged by size:
4052 436 2
BssSI C'ACGA_G
Cuts at: 2761 3119 2761
Size: 358 4132
BstAPI GCAn_nnn'nTGC
Cuts at: 1322 1867 1322
Size: 545 3945
BstBI TT'CG_AA
Cuts at: 2851 2851
Size: 4490
BstXI CCAn_nnnn'nTGG
Cuts at: 142 142
Size: 4490
BstYI r'GATC_y
Cuts at: 1 356 407 550 780 1515 1982 2005 2340
Size: 355 51 143 230 735 467 23 335
Cuts at: 2340 2586 3598 3684 3696 4042 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 735 467 449 355 346 335 246
230 143 86 51 23 12
BtgI C'CryG_G
Cuts at: 107 560 702 1478 107
Size: 453 142 776 3119
Fragments arranged by size:
3119 776 453 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1425 2440 2621 2784 1425
Size: 1015 181 163 3131
Fragments arranged by size:
3131 1015 181 163
BtsI GCAGTG_nn'
Cuts at: 1075 1733 1075
Size: 658 3832
DraI TTT'AAA
Cuts at: 1489 1489
Size: 4490
DrdI GACnn_nn'nnGTC
Cuts at: 2196 3054 2196
Size: 858 3632
EaeI y'GGCC_r
Cuts at: 595 961 1006 1271 2075 2249 2640 2667 4012
Size: 366 45 265 804 174 391 27 1345
Cuts at: 4012 595
Size: 1073
Fragments arranged by size:
1345 1073 804 391 366 265 174 45
27
EagI C'GGCC_G
Cuts at: 595 961 2075 595
Size: 366 1114 3010
Fragments arranged by size:
3010 1114 366
EarI CTCTTCn'nnn_
Cuts at: 650 790 2513 2723 650
Size: 140 1723 210 2417
Fragments arranged by size:
2417 1723 210 140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 541 2698 3008 3154 4300 541
Size: 2157 310 146 1146 731
Fragments arranged by size:
2157 1146 731 310 146
EcoICRI GAG'CTC
Cuts at: 312 1502 312
Size: 1190 3300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 382 397 942 1236 2315 2747 3494 3762
Size: 103 15 545 294 1079 432 747 268
Cuts at: 3762 279
Size: 1007
Fragments arranged by size:
1079 1007 747 545 432 294 268 103
15
EcoNI CCTnn'n_nnAGG
Cuts at: 865 865
Size: 4490
EcoO109I rG'GnC_Cy
Cuts at: 138 471 565 1035 1629 138
Size: 333 94 470 594 2999
Fragments arranged by size:
2999 594 470 333 94
EcoRI G'AATT_C
Cuts at: 1494 1494
Size: 4490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 4490
FspI TGC'GCA
Cuts at: 2271 2271
Size: 4490
HaeII r_GCGC'y
Cuts at: 249 632 1026 1083 1265 1924 2172 3194 4173
Size: 383 394 57 182 659 248 1022 979
Cuts at: 4173 249
Size: 566
Fragments arranged by size:
1022 979 659 566 394 383 248 182
57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 499 531 2332 2362 2364 2394 499
Size: 32 1801 30 2 30 2595
Fragments arranged by size:
2595 1801 32 30 30 2
HincII GTy'rAC
Cuts at: 1529 1529
Size: 4490
KasI G'GCGC_C
Cuts at: 1022 1920 2168 1022
Size: 898 248 3344
Fragments arranged by size:
3344 898 248
KpnI G_GTAC'C
Cuts at: 1510 1510
Size: 4490
MluI A'CGCG_T
Cuts at: 3923 3923
Size: 4490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3161 3345 3161
Size: 184 4306
MscI TGG'CCA
Cuts at: 2251 2251
Size: 4490
MslI CAynn'nnrTG
Cuts at: 215 1143 1367 1379 2479 2606 215
Size: 928 224 12 1100 127 2099
Fragments arranged by size:
2099 1100 928 224 127 12
MspA1I CmG'CkG
Cuts at: 323 777 1061 1088 1601 1915 2275 3288 3533
Size: 454 284 27 513 314 360 1013 245
Cuts at: 3533 323
Size: 1280
Fragments arranged by size:
1280 1013 513 454 360 314 284 245
27
NaeI GCC'GGC
Cuts at: 1021 2671 1021
Size: 1650 2840
NarI GG'CG_CC
Cuts at: 1023 1921 2169 1023
Size: 898 248 3344
Fragments arranged by size:
3344 898 248
NcoI C'CATG_G
Cuts at: 107 702 107
Size: 595 3895
NgoMIV G'CCGG_C
Cuts at: 1019 2669 1019
Size: 1650 2840
NspI r_CATG'y
Cuts at: 1543 2043 2950 1543
Size: 500 907 3083
Fragments arranged by size:
3083 907 500
PasI CC'CwG_GG
Cuts at: 142 825 975 142
Size: 683 150 3657
Fragments arranged by size:
3657 683 150
PciI A'CATG_T
Cuts at: 2946 2946
Size: 4490
PflMI CCAn_nnn'nTGG
Cuts at: 1479 1479
Size: 4490
PfoI T'CCnGG_A
Cuts at: 359 663 811 919 1031 359
Size: 304 148 108 112 3818
Fragments arranged by size:
3818 304 148 112 108
PmeI GTTT'AAAC
Cuts at: 1489 1489
Size: 4490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4458
PpuMI rG'GwC_Cy
Cuts at: 471 1035 471
Size: 564 3926
PspOMI G'GGCC_C
Cuts at: 978 978
Size: 4490
PstI C_TGCA'G
Cuts at: 543 869 1318 1537 2222 543
Size: 326 449 219 685 2811
Fragments arranged by size:
2811 685 449 326 219
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4490
PvuII CAG'CTG
Cuts at: 1061 1915 2275 1061
Size: 854 360 3276
Fragments arranged by size:
3276 854 360
RsrII CG'GwC_CG
Cuts at: 2685 2685
Size: 4490
SacI G_AGCT'C
Cuts at: 314 1504 314
Size: 1190 3300
SalI G'TCGA_C
Cuts at: 1527 1527
Size: 4490
SapI GCTCTTCn'nnn_
Cuts at: 650 2513 2723 650
Size: 1863 210 2417
Fragments arranged by size:
2417 1863 210
SbfI CC_TGCA'GG
Cuts at: 1537 1537
Size: 4490
ScaI AGT'ACT
Cuts at: 4007 4007
Size: 4490
SexAI A'CCwGG_T
Cuts at: 1184 1184
Size: 4490
SfcI C'TryA_G
Cuts at: 33 475 539 865 1314 1533 2218 3211 3402
Size: 442 64 326 449 219 685 993 191
Cuts at: 3402 33
Size: 1121
Fragments arranged by size:
1121 993 685 449 442 326 219 191
64
SfoI GGC'GCC
Cuts at: 1024 1922 2170 1024
Size: 898 248 3344
Fragments arranged by size:
3344 898 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4490
SgrAI Cr'CCGG_yG
Cuts at: 1019 1378 1019
Size: 359 4131
SmaI CCC'GGG
Cuts at: 437 745 1512 437
Size: 308 767 3415
Fragments arranged by size:
3415 767 308
SmlI C'TyrA_G
Cuts at: 1645 2560 3052 3314 1645
Size: 915 492 262 2821
Fragments arranged by size:
2821 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4490
SphI G_CATG'C
Cuts at: 1543 2043 1543
Size: 500 3990
StuI AGG'CCT
Cuts at: 292 943 292
Size: 651 3839
StyI C'CwwG_G
Cuts at: 107 119 347 461 702 1197 1624 1978 107
Size: 12 228 114 241 495 427 354 2619
Fragments arranged by size:
2619 495 427 354 241 228 114 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 408 1026 2073 2206 2665 2874 408
Size: 618 1047 133 459 209 2024
Fragments arranged by size:
2024 1047 618 459 209 133
TatI w'GTAC_w
Cuts at: 4005 4005
Size: 4490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 552 584 4007 4039 552
Size: 32 3423 32 1003
Fragments arranged by size:
3423 1003 32 32
Tth111I GACn'n_nGTC
Cuts at: 915 2287 915
Size: 1372 3118
XbaI T'CTAG_A
Cuts at: 1521 1521
Size: 4490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 494 1985 214
Size: 280 1491 2719
Fragments arranged by size:
2719 1491 280
XmaI C'CCGG_G
Cuts at: 435 743 1510 435
Size: 308 767 3415
Fragments arranged by size:
3415 767 308
XmnI GAAnn'nnTTC
Cuts at: 158 1222 1306 158
Size: 1064 84 3342
Fragments arranged by size:
3342 1064 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BmtI BsiWI BsmI BsrGI BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SnaBI SrfI SspI SwaI XhoI ZraI