Restriction map for
pF1KB7792
(Circular) (Six-base) MAPSORT of: pF1KB7792.seq Check: 1072 from: 1 to: 4670
pF1KB7792 4670 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1708 1708
Size: 4670
Acc65I G'GTAC_C
Cuts at: 1686 1686
Size: 4670
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 2927 3674 3942 2495
Size: 432 747 268 3223
Fragments arranged by size:
3223 747 432 268
AfeI AGC'GCT
Cuts at: 772 772
Size: 4670
AflIII A'CryG_T
Cuts at: 131 1586 2022 3126 4103 131
Size: 1455 436 1104 977 698
Fragments arranged by size:
1455 1104 977 698 436
AgeI A'CCGG_T
Cuts at: 1610 3058 1610
Size: 1448 3222
AleI CACnn'nnGTG
Cuts at: 713 713
Size: 4670
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 524 556 524
Size: 32 4638
AlwNI CAG_nnn'CTG
Cuts at: 1975 3542 1975
Size: 1567 3103
ApaI G_GGCC'C
Cuts at: 494 494
Size: 4670
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 949 1674 4023 4279 949
Size: 725 2349 256 1340
Fragments arranged by size:
2349 1340 725 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 208 228 369 1110 1275 1690 208
Size: 20 141 741 165 415 3188
Fragments arranged by size:
3188 741 415 165 141 20
BamHI G'GATC_C
Cuts at: 1695 1695
Size: 4670
BanI G'GyrC_C
Cuts at: 1177 1251 1686 2100 2348 2383 1177
Size: 74 435 414 248 35 3464
Fragments arranged by size:
3464 435 414 248 74 35
BanII G_rGCy'C
Cuts at: 197 494 937 1684 2714 197
Size: 297 443 747 1030 2153
Fragments arranged by size:
2153 1030 747 443 297
BbeI G_GCGC'C
Cuts at: 2104 2352 2104
Size: 248 4422
BbsI GAAGACnn'nnnn_
Cuts at: 866 866
Size: 4670
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3329 4670 2568
Size: 761 1341 2568
Fragments arranged by size:
2568 1341 761
BclI T'GATC_A
Cuts at: 2190 2190
Size: 4670
BglI GCCn_nnn'nGGC
Cuts at: 806 806
Size: 4670
BglII A'GATC_T
Cuts at: 886 2185 4222 886
Size: 1299 2037 1334
Fragments arranged by size:
2037 1334 1299
BlpI GC'TnA_GC
Cuts at: 1782 1782
Size: 4670
Bme1580I G_kGCm'C
Cuts at: 494 811 1180 1256 2295 2388 3444 494
Size: 317 369 76 1039 93 1056 1720
Fragments arranged by size:
1720 1056 1039 369 317 93 76
BmgBI CAC'GTC
Cuts at: 1530 1530
Size: 4670
BmrI ACTGGGnnnn_n'
Cuts at: 814 1882 1963 2298 4442 814
Size: 1068 81 335 2144 1042
Fragments arranged by size:
2144 1068 1042 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 234 3037 234
Size: 2803 1867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 548 161
Size: 387 4283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 1846 2725 3217 3515 1375
Size: 471 879 492 298 2530
Fragments arranged by size:
2530 879 492 471 298
BsaI GGTCTCn'nnnn_
Cuts at: 1342 4374 1342
Size: 3032 1638
BsaAI yAC'GTr
Cuts at: 1035 2023 1035
Size: 988 3682
BsaBI GATnn'nnATC
Cuts at: 6 2184 4221 6
Size: 2178 2037 455
Fragments arranged by size:
2178 2037 455
BsaHI Gr'CG_yC
Cuts at: 665 1105 1270 2101 2349 3051 4547 665
Size: 440 165 831 248 702 1496 788
Fragments arranged by size:
1496 831 788 702 440 248 165
BsaWI w'CCGG_w
Cuts at: 1610 1859 2380 3058 3332 3479 3890 1610
Size: 249 521 678 274 147 411 2390
Fragments arranged by size:
2390 678 521 411 274 249 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1336 1411 1336
Size: 75 4595
BseYI C'CCAG_C
Cuts at: 2095 2853 3430 3926 2095
Size: 758 577 496 2839
Fragments arranged by size:
2839 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1141 1120
Size: 21 4649
BsiEI CG_ry'CG
Cuts at: 104 2212 2258 3466 104
Size: 2108 46 1208 1308
Fragments arranged by size:
2108 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 503 937 1403 1517 1649 1684 2462 2652 3444
Size: 434 466 114 132 35 778 190 792
Cuts at: 3444 503
Size: 1729
Fragments arranged by size:
1729 792 778 466 434 190 132 114
35
BsmI GAATG_Cn'
Cuts at: 362 434 606 362
Size: 72 172 4426
Fragments arranged by size:
4426 172 72
Bsp1286I G_dGCh'C
Cuts at: 197 494 503 811 937 1180 1256 1403 1517
Size: 297 9 308 126 243 76 147 114
Cuts at: 1517 1649 1684 2295 2388 2462 2652 2714 3444
Size: 132 35 611 93 74 190 62 730
Cuts at: 3444 197
Size: 1423
Fragments arranged by size:
1423 730 611 308 297 243 190 147
132 126 114 93 76 74 62 35
9
BspEI T'CCGG_A
Cuts at: 3890 3890
Size: 4670
BspHI T'CATG_A
Cuts at: 3846 3846
Size: 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 593 1232 1424 1720 1934 2236 2617 593
Size: 639 192 296 214 302 381 2646
Fragments arranged by size:
2646 639 381 302 296 214 192
BsrBI CCG'CTC
Cuts at: 50 1167 2962 3016 4500 4665 50
Size: 1117 1795 54 1484 165 55
Fragments arranged by size:
1795 1484 1117 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2582 2582
Size: 4670
BsrFI r'CCGG_y
Cuts at: 1610 2668 2849 3058 1610
Size: 1058 181 209 3222
Fragments arranged by size:
3222 1058 209 181
BsrGI T'GTAC_A
Cuts at: 1490 1535 1490
Size: 45 4625
BssHII G'CGCG_C
Cuts at: 1372 1422 1372
Size: 50 4620
BssSI C'ACGA_G
Cuts at: 892 2941 3299 892
Size: 2049 358 2263
Fragments arranged by size:
2263 2049 358
BstAPI GCAn_nnn'nTGC
Cuts at: 554 1286 1589 2047 554
Size: 732 303 458 3177
Fragments arranged by size:
3177 732 458 303
BstBI TT'CG_AA
Cuts at: 3031 3031
Size: 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 1548 1548
Size: 4670
BstYI r'GATC_y
Cuts at: 1 886 1695 2162 2185 2520 2766 3778 3864
Size: 885 809 467 23 335 246 1012 86
Cuts at: 3864 3876 4222 1
Size: 12 346 449
Fragments arranged by size:
1012 885 809 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 682 682
Size: 4670
BtgI C'CryG_G
Cuts at: 107 1214 1298 1557 107
Size: 1107 84 259 3220
Fragments arranged by size:
3220 1107 259 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 203 1344 2620 2801 2964 203
Size: 1141 1276 181 163 1909
Fragments arranged by size:
1909 1276 1141 181 163
BtsI GCAGTG_nn'
Cuts at: 1503 1913 1503
Size: 410 4260
DraI TTT'AAA
Cuts at: 1669 1669
Size: 4670
DrdI GACnn_nn'nnGTC
Cuts at: 2376 3234 2376
Size: 858 3812
EaeI y'GGCC_r
Cuts at: 266 675 726 2255 2429 2820 2847 4192 266
Size: 409 51 1529 174 391 27 1345 744
Fragments arranged by size:
1529 1345 744 409 391 174 51 27
EagI C'GGCC_G
Cuts at: 2255 2255
Size: 4670
EarI CTCTTCn'nnn_
Cuts at: 296 1522 2693 2903 296
Size: 1226 1171 210 2063
Fragments arranged by size:
2063 1226 1171 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2878 3188 3334 4480 2878
Size: 310 146 1146 3068
Fragments arranged by size:
3068 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 1682 935
Size: 747 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 548 2495 2927 3674 3942 161
Size: 387 1947 432 747 268 889
Fragments arranged by size:
1947 889 747 432 387 268
EcoO109I rG'GnC_Cy
Cuts at: 213 560 966 1809 213
Size: 347 406 843 3074
Fragments arranged by size:
3074 843 406 347
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
EcoRV GAT'ATC
Cuts at: 468 468
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4670
FspI TGC'GCA
Cuts at: 2451 2451
Size: 4670
HaeII r_GCGC'y
Cuts at: 774 998 1214 2104 2352 3374 4353 774
Size: 224 216 890 248 1022 979 1091
Fragments arranged by size:
1091 1022 979 890 248 224 216
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1609 1641 2512 2542 2544 2574 1609
Size: 32 871 30 2 30 3705
Fragments arranged by size:
3705 871 32 30 30 2
HincII GTy'rAC
Cuts at: 1709 1709
Size: 4670
KasI G'GCGC_C
Cuts at: 2100 2348 2100
Size: 248 4422
KpnI G_GTAC'C
Cuts at: 1690 1690
Size: 4670
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 408 998 1214 3341 3525 408
Size: 590 216 2127 184 1553
Fragments arranged by size:
2127 1553 590 216 184
MscI TGG'CCA
Cuts at: 268 677 2431 268
Size: 409 1754 2507
Fragments arranged by size:
2507 1754 409
MslI CAynn'nnrTG
Cuts at: 713 1546 2659 2786 713
Size: 833 1113 127 2597
Fragments arranged by size:
2597 1113 833 127
MspA1I CmG'CkG
Cuts at: 1095 1155 1334 1472 1781 2095 2455 3468 3713
Size: 60 179 138 309 314 360 1013 245
Cuts at: 3713 1095
Size: 2052
Fragments arranged by size:
2052 1013 360 314 309 245 179 138
60
NaeI GCC'GGC
Cuts at: 2851 2851
Size: 4670
NarI GG'CG_CC
Cuts at: 2101 2349 2101
Size: 248 4422
NcoI C'CATG_G
Cuts at: 107 107
Size: 4670
NgoMIV G'CCGG_C
Cuts at: 2849 2849
Size: 4670
NspI r_CATG'y
Cuts at: 135 1590 1723 2223 3130 135
Size: 1455 133 500 907 1675
Fragments arranged by size:
1675 1455 907 500 133
PciI A'CATG_T
Cuts at: 131 1586 3126 131
Size: 1455 1540 1675
Fragments arranged by size:
1675 1540 1455
PflMI CCAn_nnn'nTGG
Cuts at: 1038 1038
Size: 4670
PfoI T'CCnGG_A
Cuts at: 1520 1520
Size: 4670
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PmlI CAC'GTG
Cuts at: 1035 1035
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4638
PpuMI rG'GwC_Cy
Cuts at: 213 213
Size: 4670
PspOMI G'GGCC_C
Cuts at: 490 490
Size: 4670
PstI C_TGCA'G
Cuts at: 1717 2402 1717
Size: 685 3985
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 1155 2095 2455 1155
Size: 940 360 3370
Fragments arranged by size:
3370 940 360
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 937 1684 937
Size: 747 3923
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 1522 2693 2903 1522
Size: 1171 210 3289
Fragments arranged by size:
3289 1171 210
SbfI CC_TGCA'GG
Cuts at: 1717 1717
Size: 4670
ScaI AGT'ACT
Cuts at: 4187 4187
Size: 4670
SfcI C'TryA_G
Cuts at: 33 1713 2398 3391 3582 33
Size: 1680 685 993 191 1121
Fragments arranged by size:
1680 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2102 2350 2102
Size: 248 4422
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SmaI CCC'GGG
Cuts at: 1112 1692 1112
Size: 580 4090
SmlI C'TyrA_G
Cuts at: 1390 1825 2740 3232 3494 1390
Size: 435 915 492 262 2566
Fragments arranged by size:
2566 915 492 435 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4670
SphI G_CATG'C
Cuts at: 1723 2223 1723
Size: 500 4170
StyI C'CwwG_G
Cuts at: 107 402 1238 1804 2158 107
Size: 295 836 566 354 2619
Fragments arranged by size:
2619 836 566 354 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 586 2253 2386 2845 3054 586
Size: 1667 133 459 209 2202
Fragments arranged by size:
2202 1667 459 209 133
TatI w'GTAC_w
Cuts at: 1490 1535 4185 1490
Size: 45 2650 1975
Fragments arranged by size:
2650 1975 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4187 4219 4187
Size: 32 4638
Tth111I GACn'n_nGTC
Cuts at: 259 2467 259
Size: 2208 2462
XbaI T'CTAG_A
Cuts at: 1701 1701
Size: 4670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 501 1068 1245 2165 501
Size: 567 177 920 3006
Fragments arranged by size:
3006 920 567 177
XmaI C'CCGG_G
Cuts at: 1110 1690 1110
Size: 580 4090
XmnI GAAnn'nnTTC
Cuts at: 622 622
Size: 4670
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsiWI BsmBI
BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI