Restriction map for
pF1KB7793
(Circular) (Six-base) MAPSORT of: pF1KB7793.seq Check: 9725 from: 1 to: 4493
pF1KB7793 4493 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 644 644
Size: 4493
AccI GT'mk_AC
Cuts at: 1531 1531
Size: 4493
Acc65I G'GTAC_C
Cuts at: 1509 1509
Size: 4493
AclI AA'CG_TT
Cuts at: 4333 4333
Size: 4493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1284 2318 2750 3497 3765 1284
Size: 1034 432 747 268 2012
Fragments arranged by size:
2012 1034 747 432 268
AflIII A'CryG_T
Cuts at: 1430 1845 2949 3926 1430
Size: 415 1104 977 1997
Fragments arranged by size:
1997 1104 977 415
AgeI A'CCGG_T
Cuts at: 2881 2881
Size: 4493
AhdI GACnn_n'nnGTC
Cuts at: 645 645
Size: 4493
AleI CACnn'nnGTG
Cuts at: 200 200
Size: 4493
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1040 1072 1384 1416 1040
Size: 32 312 32 4117
Fragments arranged by size:
4117 312 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1798 3365 1798
Size: 1567 2926
ApaLI G'TGCA_C
Cuts at: 3263 3263
Size: 4493
ApoI r'AATT_y
Cuts at: 987 1204 1497 3846 4102 987
Size: 217 293 2349 256 1378
Fragments arranged by size:
2349 1378 293 256 217
AseI AT'TA_AT
Cuts at: 20 20
Size: 4493
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
AvaI C'yCGr_G
Cuts at: 207 261 482 504 1146 1513 207
Size: 54 221 22 642 367 3187
Fragments arranged by size:
3187 642 367 221 54 22
BamHI G'GATC_C
Cuts at: 1277 1518 1277
Size: 241 4252
BanI G'GyrC_C
Cuts at: 1324 1509 1923 2171 2206 1324
Size: 185 414 248 35 3611
Fragments arranged by size:
3611 414 248 185 35
BanII G_rGCy'C
Cuts at: 241 1507 2537 241
Size: 1266 1030 2197
Fragments arranged by size:
2197 1266 1030
BbeI G_GCGC'C
Cuts at: 1328 1927 2175 1328
Size: 599 248 3646
Fragments arranged by size:
3646 599 248
BbsI GAAGACnn'nnnn_
Cuts at: 644 644
Size: 4493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1137 1171 90
Size: 34 1013 34 3412
Fragments arranged by size:
3412 1013 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2391 3152 4493 2391
Size: 761 1341 2391
Fragments arranged by size:
2391 1341 761
BclI T'GATC_A
Cuts at: 2013 2013
Size: 4493
BfrBI ATG'CAT
Cuts at: 901 901
Size: 4493
BglII A'GATC_T
Cuts at: 952 2008 4045 952
Size: 1056 2037 1400
Fragments arranged by size:
2037 1400 1056
BlpI GC'TnA_GC
Cuts at: 1605 1605
Size: 4493
Bme1580I G_kGCm'C
Cuts at: 2118 2211 3267 2118
Size: 93 1056 3344
Fragments arranged by size:
3344 1056 93
BmgBI CAC'GTC
Cuts at: 372 552 1433 372
Size: 180 881 3432
Fragments arranged by size:
3432 881 180
BmrI ACTGGGnnnn_n'
Cuts at: 1705 1786 2121 4265 1705
Size: 81 335 2144 1933
Fragments arranged by size:
2144 1933 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 4493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1288 1288
Size: 4493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1669 2548 3040 3338 1669
Size: 879 492 298 2824
Fragments arranged by size:
2824 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 119 4197 119
Size: 4078 415
BsaAI yAC'GTr
Cuts at: 1846 1846
Size: 4493
BsaBI GATnn'nnATC
Cuts at: 6 2007 4044 6
Size: 2001 2037 455
Fragments arranged by size:
2037 2001 455
BsaHI Gr'CG_yC
Cuts at: 641 1325 1924 2172 2874 4370 641
Size: 684 599 248 702 1496 764
Fragments arranged by size:
1496 764 702 684 599 248
BsaWI w'CCGG_w
Cuts at: 1229 1682 2203 2881 3155 3302 3713 1229
Size: 453 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 453 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 370 379 400 1040 1070 1144 1174 349
Size: 21 9 21 640 30 74 30 3668
Fragments arranged by size:
3668 640 74 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 898 1486 898
Size: 588 3905
BseYI C'CCAG_C
Cuts at: 230 234 410 476 497 1255 1918 2676 3253
Size: 4 176 66 21 758 663 758 577
Cuts at: 3253 3749 230
Size: 496 974
Fragments arranged by size:
974 758 758 663 577 496 176 66
21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 595 1141 385
Size: 210 546 3737
Fragments arranged by size:
3737 546 210
BsiEI CG_ry'CG
Cuts at: 104 547 2035 2081 3289 104
Size: 443 1488 46 1208 1308
Fragments arranged by size:
1488 1308 1208 443 46
BsiHKAI G_wGCw'C
Cuts at: 1507 2285 2475 3267 1507
Size: 778 190 792 2733
Fragments arranged by size:
2733 792 778 190
BsmI GAATG_Cn'
Cuts at: 561 895 561
Size: 334 4159
BsmBI CGTCTCn'nnnn_
Cuts at: 864 1119 864
Size: 255 4238
Bsp1286I G_dGCh'C
Cuts at: 241 1507 2118 2211 2285 2475 2537 3267 241
Size: 1266 611 93 74 190 62 730 1467
Fragments arranged by size:
1467 1266 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3713 3713
Size: 4493
BspHI T'CATG_A
Cuts at: 3669 3669
Size: 4493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1163 1543 1757 2059 2440 1163
Size: 380 214 302 381 3216
Fragments arranged by size:
3216 381 380 302 214
BsrBI CCG'CTC
Cuts at: 50 336 2785 2839 4323 4488 50
Size: 286 2449 54 1484 165 55
Fragments arranged by size:
2449 1484 286 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2405 2405
Size: 4493
BsrFI r'CCGG_y
Cuts at: 191 460 542 784 1130 2491 2672 2881 191
Size: 269 82 242 346 1361 181 209 1803
Fragments arranged by size:
1803 1361 346 269 242 209 181 82
BssSI C'ACGA_G
Cuts at: 2764 3122 2764
Size: 358 4135
BstAPI GCAn_nnn'nTGC
Cuts at: 1870 1870
Size: 4493
BstBI TT'CG_AA
Cuts at: 838 2854 838
Size: 2016 2477
BstYI r'GATC_y
Cuts at: 1 952 1212 1277 1518 1985 2008 2343 2589
Size: 951 260 65 241 467 23 335 246
Cuts at: 2589 3601 3687 3699 4045 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 951 467 449 346 335 260 246
241 86 65 23 12
BtgI C'CryG_G
Cuts at: 573 995 573
Size: 422 4071
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2443 2624 2787 2443
Size: 181 163 4149
Fragments arranged by size:
4149 181 163
BtsI GCAGTG_nn'
Cuts at: 397 1736 397
Size: 1339 3154
DraI TTT'AAA
Cuts at: 1492 1492
Size: 4493
DrdI GACnn_nn'nnGTC
Cuts at: 694 2199 3057 694
Size: 1505 858 2130
Fragments arranged by size:
2130 1505 858
EaeI y'GGCC_r
Cuts at: 544 621 1181 1331 2078 2252 2643 2670 4015
Size: 77 560 150 747 174 391 27 1345
Cuts at: 4015 544
Size: 1022
Fragments arranged by size:
1345 1022 747 560 391 174 150 77
27
EagI C'GGCC_G
Cuts at: 544 2078 544
Size: 1534 2959
EarI CTCTTCn'nnn_
Cuts at: 921 2516 2726 921
Size: 1595 210 2688
Fragments arranged by size:
2688 1595 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 529 929 2701 3011 3157 4303 529
Size: 400 1772 310 146 1146 719
Fragments arranged by size:
1772 1146 719 400 310 146
EcoICRI GAG'CTC
Cuts at: 1505 1505
Size: 4493
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1284 1288 2318 2750 3497 3765 1284
Size: 4 1030 432 747 268 2012
Fragments arranged by size:
2012 1030 747 432 268 4
EcoO109I rG'GnC_Cy
Cuts at: 253 357 1632 253
Size: 104 1275 3114
Fragments arranged by size:
3114 1275 104
EcoRI G'AATT_C
Cuts at: 1497 1497
Size: 4493
EcoRV GAT'ATC
Cuts at: 180 180
Size: 4493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1419 2512 1419
Size: 1093 3400
FspI TGC'GCA
Cuts at: 2274 2274
Size: 4493
HaeII r_GCGC'y
Cuts at: 269 1328 1381 1927 2175 3197 4176 269
Size: 1059 53 546 248 1022 979 586
Fragments arranged by size:
1059 1022 979 586 546 248 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 370 381 402 517 549 589 621 631
Size: 21 11 21 115 32 40 32 10
Cuts at: 631 663 1144 1176 2335 2365 2367 2397 349
Size: 32 481 32 1159 30 2 30 2445
Fragments arranged by size:
2445 1159 481 115 40 32 32 32
32 30 30 21 21 11 10 2
HincII GTy'rAC
Cuts at: 375 780 1532 375
Size: 405 752 3336
Fragments arranged by size:
3336 752 405
HindIII A'AGCT_T
Cuts at: 151 151
Size: 4493
KasI G'GCGC_C
Cuts at: 1324 1923 2171 1324
Size: 599 248 3646
Fragments arranged by size:
3646 599 248
KpnI G_GTAC'C
Cuts at: 1513 1513
Size: 4493
MluI A'CGCG_T
Cuts at: 3926 3926
Size: 4493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 275 3164 3348 275
Size: 2889 184 1420
Fragments arranged by size:
2889 1420 184
MscI TGG'CCA
Cuts at: 1183 2254 1183
Size: 1071 3422
MslI CAynn'nnrTG
Cuts at: 200 2482 2609 200
Size: 2282 127 2084
Fragments arranged by size:
2282 2084 127
MspA1I CmG'CkG
Cuts at: 234 276 438 671 997 1604 1918 2278 3291
Size: 42 162 233 326 607 314 360 1013
Cuts at: 3291 3536 234
Size: 245 1191
Fragments arranged by size:
1191 1013 607 360 326 314 245 233
162 42
NaeI GCC'GGC
Cuts at: 462 544 2674 462
Size: 82 2130 2281
Fragments arranged by size:
2281 2130 82
NarI GG'CG_CC
Cuts at: 1325 1924 2172 1325
Size: 599 248 3646
Fragments arranged by size:
3646 599 248
NgoMIV G'CCGG_C
Cuts at: 460 542 2672 460
Size: 82 2130 2281
Fragments arranged by size:
2281 2130 82
NsiI A_TGCA'T
Cuts at: 903 903
Size: 4493
NspI r_CATG'y
Cuts at: 774 1546 2046 2953 774
Size: 772 500 907 2314
Fragments arranged by size:
2314 907 772 500
PasI CC'CwG_GG
Cuts at: 422 422
Size: 4493
PciI A'CATG_T
Cuts at: 2949 2949
Size: 4493
PflMI CCAn_nnn'nTGG
Cuts at: 579 579
Size: 4493
PfoI T'CCnGG_A
Cuts at: 627 1265 627
Size: 638 3855
PmeI GTTT'AAAC
Cuts at: 1492 1492
Size: 4493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1040 1072 3632 3664 1040
Size: 32 2560 32 1869
Fragments arranged by size:
2560 1869 32 32
PpuMI rG'GwC_Cy
Cuts at: 253 357 253
Size: 104 4389
PshAI GACnn'nnGTC
Cuts at: 876 876
Size: 4493
PspXI vC'TCGA_Gb
Cuts at: 261 261
Size: 4493
PstI C_TGCA'G
Cuts at: 1361 1540 2225 1361
Size: 179 685 3629
Fragments arranged by size:
3629 685 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4493
PvuII CAG'CTG
Cuts at: 234 1918 2278 234
Size: 1684 360 2449
Fragments arranged by size:
2449 1684 360
RsrII CG'GwC_CG
Cuts at: 537 2688 537
Size: 2151 2342
SacI G_AGCT'C
Cuts at: 1507 1507
Size: 4493
SacII CC_GC'GG
Cuts at: 998 998
Size: 4493
SalI G'TCGA_C
Cuts at: 1530 1530
Size: 4493
SapI GCTCTTCn'nnn_
Cuts at: 921 2516 2726 921
Size: 1595 210 2688
Fragments arranged by size:
2688 1595 210
SbfI CC_TGCA'GG
Cuts at: 1540 1540
Size: 4493
ScaI AGT'ACT
Cuts at: 4010 4010
Size: 4493
SfcI C'TryA_G
Cuts at: 33 1357 1536 2221 3214 3405 33
Size: 1324 179 685 993 191 1121
Fragments arranged by size:
1324 1121 993 685 191 179
SfoI GGC'GCC
Cuts at: 1326 1925 2173 1326
Size: 599 248 3646
Fragments arranged by size:
3646 599 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4493
SgrAI Cr'CCGG_yG
Cuts at: 784 784
Size: 4493
SmaI CCC'GGG
Cuts at: 1515 1515
Size: 4493
SmlI C'TyrA_G
Cuts at: 261 1648 2563 3055 3317 261
Size: 1387 915 492 262 1437
Fragments arranged by size:
1437 1387 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4493
SphI G_CATG'C
Cuts at: 1546 2046 1546
Size: 500 3993
StyI C'CwwG_G
Cuts at: 249 1627 1981 249
Size: 1378 354 2761
Fragments arranged by size:
2761 1378 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 525 583 2076 2209 2668 2877 525
Size: 58 1493 133 459 209 2141
Fragments arranged by size:
2141 1493 459 209 133 58
TatI w'GTAC_w
Cuts at: 842 4008 842
Size: 3166 1327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 4461
Tth111I GACn'n_nGTC
Cuts at: 2290 2290
Size: 4493
XbaI T'CTAG_A
Cuts at: 1524 1524
Size: 4493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1263 1988 1263
Size: 725 3768
XhoI C'TCGA_G
Cuts at: 261 261
Size: 4493
XmaI C'CCGG_G
Cuts at: 1513 1513
Size: 4493
XmnI GAAnn'nnTTC
Cuts at: 1055 1055
Size: 4493
ZraI GAC'GTC
Cuts at: 642 642
Size: 4493
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI ApaI AscI AvrII BaeI
BbvCI BglI BmtI Bpu10I BsiWI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NcoI NdeI NheI NotI NruI
PacI PmlI PsiI PspOMI PsrI SanDI SexAI SfiI
SnaBI SrfI SspI StuI SwaI