Restriction map for
pF1KB7804
(Circular) (Six-base) MAPSORT of: pF1KB7804.seq Check: 9379 from: 1 to: 4886
pF1KB7804 4886 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1828 1828
Size: 4886
AccI GT'mk_AC
Cuts at: 754 1924 754
Size: 1170 3716
Acc65I G'GTAC_C
Cuts at: 994 1902 994
Size: 908 3978
AclI AA'CG_TT
Cuts at: 4726 4726
Size: 4886
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1330 2711 3143 3890 4158 427
Size: 903 1381 432 747 268 1155
Fragments arranged by size:
1381 1155 903 747 432 268
AfeI AGC'GCT
Cuts at: 1145 1145
Size: 4886
AflIII A'CryG_T
Cuts at: 800 2238 3342 4319 800
Size: 1438 1104 977 1367
Fragments arranged by size:
1438 1367 1104 977
AgeI A'CCGG_T
Cuts at: 3274 3274
Size: 4886
AleI CACnn'nnGTG
Cuts at: 1208 1208
Size: 4886
AlwNI CAG_nnn'CTG
Cuts at: 596 793 2191 3758 596
Size: 197 1398 1567 1724
Fragments arranged by size:
1724 1567 1398 197
ApaLI G'TGCA_C
Cuts at: 3656 3656
Size: 4886
ApoI r'AATT_y
Cuts at: 428 673 1416 1890 4239 4495 428
Size: 245 743 474 2349 256 819
Fragments arranged by size:
2349 819 743 474 256 245
AseI AT'TA_AT
Cuts at: 20 20
Size: 4886
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4886
AvaI C'yCGr_G
Cuts at: 257 912 1719 1906 257
Size: 655 807 187 3237
Fragments arranged by size:
3237 807 655 187
BamHI G'GATC_C
Cuts at: 1911 1911
Size: 4886
BanI G'GyrC_C
Cuts at: 205 994 1202 1902 2316 2564 2599 205
Size: 789 208 700 414 248 35 2492
Fragments arranged by size:
2492 789 700 414 248 208 35
BanII G_rGCy'C
Cuts at: 264 840 1900 2930 264
Size: 576 1060 1030 2220
Fragments arranged by size:
2220 1060 1030 576
BbeI G_GCGC'C
Cuts at: 209 2320 2568 209
Size: 2111 248 2527
Fragments arranged by size:
2527 2111 248
BciVI GTATCCnnnnn_n'
Cuts at: 1570 2784 3545 4886 1570
Size: 1214 761 1341 1570
Fragments arranged by size:
1570 1341 1214 761
BclI T'GATC_A
Cuts at: 947 1019 2406 947
Size: 72 1387 3427
Fragments arranged by size:
3427 1387 72
BglI GCCn_nnn'nGGC
Cuts at: 229 229
Size: 4886
BglII A'GATC_T
Cuts at: 2401 4438 2401
Size: 2037 2849
BlpI GC'TnA_GC
Cuts at: 1632 1998 1632
Size: 366 4520
Bme1580I G_kGCm'C
Cuts at: 2511 2604 3660 2511
Size: 93 1056 3737
Fragments arranged by size:
3737 1056 93
BmgBI CAC'GTC
Cuts at: 194 194
Size: 4886
BmrI ACTGGGnnnn_n'
Cuts at: 2098 2179 2514 4658 2098
Size: 81 335 2144 2326
Fragments arranged by size:
2326 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 586 654 1547 3253 586
Size: 68 893 1706 2219
Fragments arranged by size:
2219 1706 893 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1029
Size: 4886
Bpu10I CC'TnA_GC
Cuts at: 1217 1217
Size: 4886
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1476 2062 2941 3433 3731 1476
Size: 586 879 492 298 2631
Fragments arranged by size:
2631 879 586 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1758 4590 1758
Size: 2832 2054
BsaAI yAC'GTr
Cuts at: 489 724 1518 2239 489
Size: 235 794 721 3136
Fragments arranged by size:
3136 794 721 235
BsaBI GATnn'nnATC
Cuts at: 6 2400 4437 6
Size: 2394 2037 455
Fragments arranged by size:
2394 2037 455
BsaHI Gr'CG_yC
Cuts at: 206 221 2317 2565 3267 4763 206
Size: 15 2096 248 702 1496 329
Fragments arranged by size:
2096 1496 702 329 248 15
BsaWI w'CCGG_w
Cuts at: 2075 2596 3274 3548 3695 4106 2075
Size: 521 678 274 147 411 2855
Fragments arranged by size:
2855 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1537 1567 1537
Size: 30 4856
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1016 1043 1674 1707 1016
Size: 27 631 33 4195
Fragments arranged by size:
4195 631 33 27
BseYI C'CCAG_C
Cuts at: 165 1535 2311 3069 3646 4142 165
Size: 1370 776 758 577 496 909
Fragments arranged by size:
1370 909 776 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 1063 1806 609
Size: 454 743 3689
Fragments arranged by size:
3689 743 454
BsiEI CG_ry'CG
Cuts at: 104 1322 2428 2474 3682 104
Size: 1218 1106 46 1208 1308
Fragments arranged by size:
1308 1218 1208 1106 46
BsiHKAI G_wGCw'C
Cuts at: 1528 1900 2678 2868 3660 1528
Size: 372 778 190 792 2754
Fragments arranged by size:
2754 792 778 372 190
BsmI GAATG_Cn'
Cuts at: 714 714
Size: 4886
Bsp1286I G_dGCh'C
Cuts at: 264 840 1528 1900 2511 2604 2678 2868 2930
Size: 576 688 372 611 93 74 190 62
Cuts at: 2930 3660 264
Size: 730 1490
Fragments arranged by size:
1490 730 688 611 576 372 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4106 4106
Size: 4886
BspHI T'CATG_A
Cuts at: 4062 4062
Size: 4886
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1828 1936 2150 2452 2833 1828
Size: 108 214 302 381 3881
Fragments arranged by size:
3881 381 302 214 108
BsrBI CCG'CTC
Cuts at: 50 242 1318 3178 3232 4716 4881 50
Size: 192 1076 1860 54 1484 165 55
Fragments arranged by size:
1860 1484 1076 192 165 55 54
BsrDI GCAATG_nn'
Cuts at: 465 1325 1598 2798 465
Size: 860 273 1200 2553
Fragments arranged by size:
2553 1200 860 273
BsrFI r'CCGG_y
Cuts at: 437 2884 3065 3274 437
Size: 2447 181 209 2049
Fragments arranged by size:
2447 2049 209 181
BsrGI T'GTAC_A
Cuts at: 1340 1340
Size: 4886
BssSI C'ACGA_G
Cuts at: 1563 3157 3515 1563
Size: 1594 358 2934
Fragments arranged by size:
2934 1594 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2263 2263
Size: 4886
BstBI TT'CG_AA
Cuts at: 3247 3247
Size: 4886
BstEII G'GTnAC_C
Cuts at: 1210 1210
Size: 4886
BstXI CCAn_nnnn'nTGG
Cuts at: 139 139
Size: 4886
BstYI r'GATC_y
Cuts at: 1 642 1257 1911 2378 2401 2736 2982 3994
Size: 641 615 654 467 23 335 246 1012
Cuts at: 3994 4080 4092 4438 1
Size: 86 12 346 449
Fragments arranged by size:
1012 654 641 615 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 350 1676 350
Size: 1326 3560
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2836 3017 3180 2836
Size: 181 163 4542
Fragments arranged by size:
4542 181 163
BtsI GCAGTG_nn'
Cuts at: 2129 2129
Size: 4886
ClaI AT'CG_AT
Cuts at: 1163 1163
Size: 4886
DraI TTT'AAA
Cuts at: 1885 1885
Size: 4886
DrdI GACnn_nn'nnGTC
Cuts at: 2592 3450 2592
Size: 858 4028
EaeI y'GGCC_r
Cuts at: 774 1319 1436 2471 2645 3036 3063 4408 774
Size: 545 117 1035 174 391 27 1345 1252
Fragments arranged by size:
1345 1252 1035 545 391 174 117 27
EagI C'GGCC_G
Cuts at: 1319 2471 1319
Size: 1152 3734
EarI CTCTTCn'nnn_
Cuts at: 152 1306 1765 2909 3119 152
Size: 1154 459 1144 210 1919
Fragments arranged by size:
1919 1154 1144 459 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 846 3094 3404 3550 4696 846
Size: 2248 310 146 1146 1036
Fragments arranged by size:
2248 1146 1036 310 146
EcoICRI GAG'CTC
Cuts at: 1898 1898
Size: 4886
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1029 1330 2711 3143 3890 4158 427
Size: 602 301 1381 432 747 268 1155
Fragments arranged by size:
1381 1155 747 602 432 301 268
EcoO109I rG'GnC_Cy
Cuts at: 1335 2025 1335
Size: 690 4196
EcoRI G'AATT_C
Cuts at: 428 1416 1890 428
Size: 988 474 3424
Fragments arranged by size:
3424 988 474
EcoRV GAT'ATC
Cuts at: 1856 1856
Size: 4886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 4886
FspI TGC'GCA
Cuts at: 2667 2667
Size: 4886
HaeII r_GCGC'y
Cuts at: 209 1147 2320 2568 3590 4569 209
Size: 938 1173 248 1022 979 526
Fragments arranged by size:
1173 1022 979 938 526 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 728 760 1011 1038 1043 1070 1857 1889 2728
Size: 32 251 27 5 27 787 32 839
Cuts at: 2728 2758 2760 2790 728
Size: 30 2 30 2824
Fragments arranged by size:
2824 839 787 251 32 32 30 30
27 27 5 2
HincII GTy'rAC
Cuts at: 1925 1925
Size: 4886
KasI G'GCGC_C
Cuts at: 205 2316 2564 205
Size: 2111 248 2527
Fragments arranged by size:
2527 2111 248
KpnI G_GTAC'C
Cuts at: 998 1906 998
Size: 908 3978
MluI A'CGCG_T
Cuts at: 4319 4319
Size: 4886
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 156 326 1727 3557 3741 156
Size: 170 1401 1830 184 1301
Fragments arranged by size:
1830 1401 1301 184 170
MscI TGG'CCA
Cuts at: 776 2647 776
Size: 1871 3015
MslI CAynn'nnrTG
Cuts at: 1039 1208 1567 2875 3002 1039
Size: 169 359 1308 127 2923
Fragments arranged by size:
2923 1308 359 169 127
MspA1I CmG'CkG
Cuts at: 165 173 596 1636 1997 2311 2671 3684 3929
Size: 8 423 1040 361 314 360 1013 245
Cuts at: 3929 165
Size: 1122
Fragments arranged by size:
1122 1040 1013 423 361 360 314 245
8
NaeI GCC'GGC
Cuts at: 3067 3067
Size: 4886
NarI GG'CG_CC
Cuts at: 206 2317 2565 206
Size: 2111 248 2527
Fragments arranged by size:
2527 2111 248
NcoI C'CATG_G
Cuts at: 1676 1676
Size: 4886
NgoMIV G'CCGG_C
Cuts at: 3065 3065
Size: 4886
NspI r_CATG'y
Cuts at: 732 804 886 1939 2439 3346 732
Size: 72 82 1053 500 907 2272
Fragments arranged by size:
2272 1053 907 500 82 72
PciI A'CATG_T
Cuts at: 800 3342 800
Size: 2542 2344
PflMI CCAn_nnn'nTGG
Cuts at: 351 747 783 351
Size: 396 36 4454
Fragments arranged by size:
4454 396 36
PmeI GTTT'AAAC
Cuts at: 1885 1885
Size: 4886
PmlI CAC'GTG
Cuts at: 724 724
Size: 4886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 4025
Size: 32 4854
PstI C_TGCA'G
Cuts at: 1711 1933 2618 1711
Size: 222 685 3979
Fragments arranged by size:
3979 685 222
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4886
PvuII CAG'CTG
Cuts at: 1636 2311 2671 1636
Size: 675 360 3851
Fragments arranged by size:
3851 675 360
RsrII CG'GwC_CG
Cuts at: 3081 3081
Size: 4886
SacI G_AGCT'C
Cuts at: 1900 1900
Size: 4886
SalI G'TCGA_C
Cuts at: 1923 1923
Size: 4886
SapI GCTCTTCn'nnn_
Cuts at: 152 2909 3119 152
Size: 2757 210 1919
Fragments arranged by size:
2757 1919 210
SbfI CC_TGCA'GG
Cuts at: 1933 1933
Size: 4886
ScaI AGT'ACT
Cuts at: 4403 4403
Size: 4886
SfcI C'TryA_G
Cuts at: 33 453 1707 1929 2614 3607 3798 33
Size: 420 1254 222 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 420 222 191
SfoI GGC'GCC
Cuts at: 207 2318 2566 207
Size: 2111 248 2527
Fragments arranged by size:
2527 2111 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4886
SmaI CCC'GGG
Cuts at: 259 1908 259
Size: 1649 3237
SmlI C'TyrA_G
Cuts at: 1491 2041 2956 3448 3710 1491
Size: 550 915 492 262 2667
Fragments arranged by size:
2667 915 550 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4886
SphI G_CATG'C
Cuts at: 1939 2439 1939
Size: 500 4386
SrfI GCCC'GGGC
Cuts at: 259 259
Size: 4886
StyI C'CwwG_G
Cuts at: 1676 2020 2374 1676
Size: 344 354 4188
Fragments arranged by size:
4188 354 344
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 2469 2602 3061 3270 1380
Size: 1089 133 459 209 2996
Fragments arranged by size:
2996 1089 459 209 133
TatI w'GTAC_w
Cuts at: 1340 4401 1340
Size: 3061 1825
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4403 4435 4403
Size: 32 4854
Tth111I GACn'n_nGTC
Cuts at: 2683 2683
Size: 4886
XbaI T'CTAG_A
Cuts at: 1917 1917
Size: 4886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1674 2381 1674
Size: 707 4179
XmaI C'CCGG_G
Cuts at: 257 1906 257
Size: 1649 3237
XmnI GAAnn'nnTTC
Cuts at: 676 1304 1420 676
Size: 628 116 4142
Fragments arranged by size:
4142 628 116
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI BsiWI BsmBI
BssHII BstZ17I Bsu36I CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI ZraI