Restriction map for
pF1KB7805
(Circular) (Six-base) MAPSORT of: pF1KB7805.seq Check: 6925 from: 1 to: 4679
pF1KB7805 4679 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 131 131
Size: 4679
AccI GT'mk_AC
Cuts at: 1717 1717
Size: 4679
Acc65I G'GTAC_C
Cuts at: 1695 1695
Size: 4679
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1123 1185 1437 2504 2936 3683 3951 431
Size: 692 62 252 1067 432 747 268 1159
Fragments arranged by size:
1159 1067 747 692 432 268 252 62
AflIII A'CryG_T
Cuts at: 1429 2031 3135 4112 1429
Size: 602 1104 977 1996
Fragments arranged by size:
1996 1104 977 602
AgeI A'CCGG_T
Cuts at: 3067 3067
Size: 4679
AlwNI CAG_nnn'CTG
Cuts at: 1984 3551 1984
Size: 1567 3112
ApaI G_GGCC'C
Cuts at: 219 219
Size: 4679
ApaLI G'TGCA_C
Cuts at: 3449 3449
Size: 4679
ApoI r'AATT_y
Cuts at: 701 731 815 899 1151 1235 1319 1403 1487
Size: 30 84 84 252 84 84 84 84
Cuts at: 1487 1683 4032 4288 701
Size: 196 2349 256 1092
Fragments arranged by size:
2349 1092 256 252 196 84 84 84
84 84 84 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 1699 1699
Size: 4679
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 871 904 871
Size: 33 4646
BamHI G'GATC_C
Cuts at: 1704 1704
Size: 4679
BanI G'GyrC_C
Cuts at: 1695 2109 2357 2392 1695
Size: 414 248 35 3982
Fragments arranged by size:
3982 414 248 35
BanII G_rGCy'C
Cuts at: 205 219 589 877 1693 2723 205
Size: 14 370 288 816 1030 2161
Fragments arranged by size:
2161 1030 816 370 288 14
BbeI G_GCGC'C
Cuts at: 2113 2361 2113
Size: 248 4431
BbsI GAAGACnn'nnnn_
Cuts at: 657 657
Size: 4679
BciVI GTATCCnnnnn_n'
Cuts at: 2577 3338 4679 2577
Size: 761 1341 2577
Fragments arranged by size:
2577 1341 761
BclI T'GATC_A
Cuts at: 603 2199 603
Size: 1596 3083
BglI GCCn_nnn'nGGC
Cuts at: 214 214
Size: 4679
BglII A'GATC_T
Cuts at: 2194 4231 2194
Size: 2037 2642
BlpI GC'TnA_GC
Cuts at: 199 1791 199
Size: 1592 3087
Bme1580I G_kGCm'C
Cuts at: 219 1047 2304 2397 3453 219
Size: 828 1257 93 1056 1445
Fragments arranged by size:
1445 1257 1056 828 93
BmgBI CAC'GTC
Cuts at: 231 231
Size: 4679
BmrI ACTGGGnnnn_n'
Cuts at: 274 1421 1891 1972 2307 4451 274
Size: 1147 470 81 335 2144 502
Fragments arranged by size:
2144 1147 502 470 335 81
BmtI G_CTAG'C
Cuts at: 479 479
Size: 4679
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 547 608 698 3046 547
Size: 61 90 2348 2180
Fragments arranged by size:
2348 2180 90 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 362 505 929 1013 1181 1265 1349 259
Size: 103 143 424 84 168 84 84 3589
Fragments arranged by size:
3589 424 168 143 103 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1855 2734 3226 3524 1855
Size: 879 492 298 3010
Fragments arranged by size:
3010 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 4383 264
Size: 4119 560
BsaAI yAC'GTr
Cuts at: 887 2032 887
Size: 1145 3534
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 2110 2358 3060 4556 2110
Size: 248 702 1496 2233
Fragments arranged by size:
2233 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3341 3488 3899 1868
Size: 521 678 274 147 411 2648
Fragments arranged by size:
2648 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1658 1688 1658
Size: 30 4649
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 867 867
Size: 4679
BseYI C'CCAG_C
Cuts at: 299 2104 2862 3439 3935 299
Size: 1805 758 577 496 1043
Fragments arranged by size:
1805 1043 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 1059
Size: 4679
BsiEI CG_ry'CG
Cuts at: 104 2221 2267 3475 104
Size: 2117 46 1208 1308
Fragments arranged by size:
2117 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 580 589 877 1693 2471 2661 3453 580
Size: 9 288 816 778 190 792 1806
Fragments arranged by size:
1806 816 792 778 288 190 9
BsmI GAATG_Cn'
Cuts at: 931 931
Size: 4679
Bsp1286I G_dGCh'C
Cuts at: 205 219 580 589 877 1047 1693 2304 2397
Size: 14 361 9 288 170 646 611 93
Cuts at: 2397 2471 2661 2723 3453 205
Size: 74 190 62 730 1431
Fragments arranged by size:
1431 730 646 611 361 288 190 170
93 74 62 14 9
BspEI T'CCGG_A
Cuts at: 3899 3899
Size: 4679
BspHI T'CATG_A
Cuts at: 3855 3855
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 131 1729 1943 2245 2626 131
Size: 1598 214 302 381 2184
Fragments arranged by size:
2184 1598 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 499 1104 2591 499
Size: 605 1487 2587
Fragments arranged by size:
2587 1487 605
BsrFI r'CCGG_y
Cuts at: 2677 2858 3067 2677
Size: 181 209 4289
Fragments arranged by size:
4289 209 181
BsrGI T'GTAC_A
Cuts at: 221 221
Size: 4679
BssSI C'ACGA_G
Cuts at: 2950 3308 2950
Size: 358 4321
BstAPI GCAn_nnn'nTGC
Cuts at: 2056 2056
Size: 4679
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstEII G'GTnAC_C
Cuts at: 147 147
Size: 4679
BstYI r'GATC_y
Cuts at: 1 1704 2171 2194 2529 2775 3787 3873 3885
Size: 1703 467 23 335 246 1012 86 12
Cuts at: 3885 4231 1
Size: 346 449
Fragments arranged by size:
1703 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 131 107
Size: 24 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 1922 1922
Size: 4679
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 918 953 1086 1121 918
Size: 35 133 35 4476
Fragments arranged by size:
4476 133 35 35
DraI TTT'AAA
Cuts at: 1008 1678 1008
Size: 670 4009
DrdI GACnn_nn'nnGTC
Cuts at: 2385 3243 2385
Size: 858 3821
EaeI y'GGCC_r
Cuts at: 2264 2438 2829 2856 4201 2264
Size: 174 391 27 1345 2742
Fragments arranged by size:
2742 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2264 2264
Size: 4679
EarI CTCTTCn'nnn_
Cuts at: 179 599 1218 2702 2912 179
Size: 420 619 1484 210 1946
Fragments arranged by size:
1946 1484 619 420 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2887 3197 3343 4489 2887
Size: 310 146 1146 3077
Fragments arranged by size:
3077 1146 310 146
EcoICRI GAG'CTC
Cuts at: 587 875 1691 587
Size: 288 816 3575
Fragments arranged by size:
3575 816 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 362 431 505 929 1013 1123 1181 1185
Size: 103 69 74 424 84 110 58 4
Cuts at: 1185 1265 1349 1437 2504 2936 3683 3951 259
Size: 80 84 88 1067 432 747 268 987
Fragments arranged by size:
1067 987 747 432 424 268 110 103
88 84 84 80 74 69 58 4
EcoO109I rG'GnC_Cy
Cuts at: 215 216 277 1818 215
Size: 1 61 1541 3076
Fragments arranged by size:
3076 1541 61 1
EcoRI G'AATT_C
Cuts at: 815 1151 1235 1403 1487 1683 815
Size: 336 84 168 84 196 3811
Fragments arranged by size:
3811 336 196 168 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4679
FspI TGC'GCA
Cuts at: 2460 2460
Size: 4679
HaeII r_GCGC'y
Cuts at: 2113 2361 3383 4362 2113
Size: 248 1022 979 2430
Fragments arranged by size:
2430 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1282 1314 1548 1580 2521 2551 2553 2583 1282
Size: 32 234 32 941 30 2 30 3378
Fragments arranged by size:
3378 941 234 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1718 1718
Size: 4679
HindIII A'AGCT_T
Cuts at: 944 1280 1616 944
Size: 336 336 4007
Fragments arranged by size:
4007 336 336
KasI G'GCGC_C
Cuts at: 2109 2357 2109
Size: 248 4431
KpnI G_GTAC'C
Cuts at: 1699 1699
Size: 4679
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3350 3534 3350
Size: 184 4495
MscI TGG'CCA
Cuts at: 2440 2440
Size: 4679
MslI CAynn'nnrTG
Cuts at: 2668 2795 2668
Size: 127 4552
MspA1I CmG'CkG
Cuts at: 198 1790 2104 2464 3477 3722 198
Size: 1592 314 360 1013 245 1155
Fragments arranged by size:
1592 1155 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2860 2860
Size: 4679
NarI GG'CG_CC
Cuts at: 2110 2358 2110
Size: 248 4431
NcoI C'CATG_G
Cuts at: 107 131 107
Size: 24 4655
NdeI CA'TA_TG
Cuts at: 1438 1438
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 2858 2858
Size: 4679
NheI G'CTAG_C
Cuts at: 475 475
Size: 4679
NspI r_CATG'y
Cuts at: 1433 1732 2232 3139 1433
Size: 299 500 907 2973
Fragments arranged by size:
2973 907 500 299
PciI A'CATG_T
Cuts at: 1429 3135 1429
Size: 1706 2973
PfoI T'CCnGG_A
Cuts at: 137 571 137
Size: 434 4245
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4647
PpuMI rG'GwC_Cy
Cuts at: 277 277
Size: 4679
PsiI TTA'TAA
Cuts at: 1259 1259
Size: 4679
PspOMI G'GGCC_C
Cuts at: 215 215
Size: 4679
PstI C_TGCA'G
Cuts at: 1726 2411 1726
Size: 685 3994
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 198 2104 2464 198
Size: 1906 360 2413
Fragments arranged by size:
2413 1906 360
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 589 877 1693 589
Size: 288 816 3575
Fragments arranged by size:
3575 816 288
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 2702 2912 2702
Size: 210 4469
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 4196 4196
Size: 4679
SexAI A'CCwGG_T
Cuts at: 254 254
Size: 4679
SfcI C'TryA_G
Cuts at: 33 246 1722 2407 3400 3591 33
Size: 213 1476 685 993 191 1121
Fragments arranged by size:
1476 1121 993 685 213 191
SfoI GGC'GCC
Cuts at: 2111 2359 2111
Size: 248 4431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 1834 2749 3241 3503 1834
Size: 915 492 262 3010
Fragments arranged by size:
3010 915 492 262
SnaBI TAC'GTA
Cuts at: 887 887
Size: 4679
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
StuI AGG'CCT
Cuts at: 862 1198 862
Size: 336 4343
StyI C'CwwG_G
Cuts at: 107 131 1813 2167 107
Size: 24 1682 354 2619
Fragments arranged by size:
2619 1682 354 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 2262 2395 2854 3063 193
Size: 2069 133 459 209 1809
Fragments arranged by size:
2069 1809 459 209 133
TatI w'GTAC_w
Cuts at: 221 1126 4194 221
Size: 905 3068 706
Fragments arranged by size:
3068 905 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 4647
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 1710 1710
Size: 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2174
Size: 4679
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
XmnI GAAnn'nnTTC
Cuts at: 1198 1282 1198
Size: 84 4595
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbvCI BcgI BfrBI Bpu10I BsiWI BsmBI BssHII
BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI SwaI XhoI ZraI