Restriction map for
pF1KB7809
(Circular) (Six-base) MAPSORT of: pF1KB7809.seq Check: 5321 from: 1 to: 4694
pF1KB7809 4694 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 363 363
Size: 4694
AccI GT'mk_AC
Cuts at: 1732 1732
Size: 4694
Acc65I G'GTAC_C
Cuts at: 1710 1710
Size: 4694
AclI AA'CG_TT
Cuts at: 4534 4534
Size: 4694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1371 1539 2519 2951 3698 3966 247
Size: 1124 168 980 432 747 268 975
Fragments arranged by size:
1124 980 975 747 432 268 168
AflIII A'CryG_T
Cuts at: 587 775 1445 1474 1531 2046 3150 4127 587
Size: 188 670 29 57 515 1104 977 1154
Fragments arranged by size:
1154 1104 977 670 515 188 57 29
AgeI A'CCGG_T
Cuts at: 3082 3082
Size: 4694
AlwNI CAG_nnn'CTG
Cuts at: 471 1999 3566 471
Size: 1528 1567 1599
Fragments arranged by size:
1599 1567 1528
ApaI G_GGCC'C
Cuts at: 154 249 154
Size: 95 4599
ApaLI G'TGCA_C
Cuts at: 220 1327 1579 3464 220
Size: 1107 252 1885 1450
Fragments arranged by size:
1885 1450 1107 252
ApoI r'AATT_y
Cuts at: 1337 1421 1505 1698 4047 4303 1337
Size: 84 84 193 2349 256 1728
Fragments arranged by size:
2349 1728 256 193 84 84
AseI AT'TA_AT
Cuts at: 20 20
Size: 4694
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
AvaI C'yCGr_G
Cuts at: 1714 1714
Size: 4694
BamHI G'GATC_C
Cuts at: 1169 1719 1169
Size: 550 4144
BanI G'GyrC_C
Cuts at: 351 1076 1710 2124 2372 2407 351
Size: 725 634 414 248 35 2638
Fragments arranged by size:
2638 725 634 414 248 35
BanII G_rGCy'C
Cuts at: 154 249 1147 1708 2738 154
Size: 95 898 561 1030 2110
Fragments arranged by size:
2110 1030 898 561 95
BbeI G_GCGC'C
Cuts at: 2128 2376 2128
Size: 248 4446
BbsI GAAGACnn'nnnn_
Cuts at: 253 253
Size: 4694
BciVI GTATCCnnnnn_n'
Cuts at: 2592 3353 4694 2592
Size: 761 1341 2592
Fragments arranged by size:
2592 1341 761
BclI T'GATC_A
Cuts at: 2214 2214
Size: 4694
BglII A'GATC_T
Cuts at: 1434 2209 4246 1434
Size: 775 2037 1882
Fragments arranged by size:
2037 1882 775
BlpI GC'TnA_GC
Cuts at: 1806 1806
Size: 4694
Bme1580I G_kGCm'C
Cuts at: 154 224 249 1331 1583 2319 2412 3468 154
Size: 70 25 1082 252 736 93 1056 1380
Fragments arranged by size:
1380 1082 1056 736 252 93 70 25
BmrI ACTGGGnnnn_n'
Cuts at: 148 244 1187 1607 1906 1987 2322 4466 148
Size: 96 943 420 299 81 335 2144 376
Fragments arranged by size:
2144 943 420 376 335 299 96 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 4694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 725 863 436
Size: 289 138 4267
Fragments arranged by size:
4267 289 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 533 1870 2749 3241 3539 533
Size: 1337 879 492 298 1688
Fragments arranged by size:
1688 1337 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4398 4398
Size: 4694
BsaAI yAC'GTr
Cuts at: 776 1446 2047 776
Size: 670 601 3423
Fragments arranged by size:
3423 670 601
BsaBI GATnn'nnATC
Cuts at: 6 2208 4245 6
Size: 2202 2037 455
Fragments arranged by size:
2202 2037 455
BsaHI Gr'CG_yC
Cuts at: 2125 2373 3075 4571 2125
Size: 248 702 1496 2248
Fragments arranged by size:
2248 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1883 2404 3082 3356 3503 3914 1883
Size: 521 678 274 147 411 2663
Fragments arranged by size:
2663 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 187
Size: 4694
BseYI C'CCAG_C
Cuts at: 752 2119 2877 3454 3950 752
Size: 1367 758 577 496 1496
Fragments arranged by size:
1496 1367 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1652 274
Size: 1378 3316
BsiEI CG_ry'CG
Cuts at: 104 2236 2282 3490 104
Size: 2132 46 1208 1308
Fragments arranged by size:
2132 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 224 1147 1331 1583 1708 2486 2676 3468 224
Size: 923 184 252 125 778 190 792 1450
Fragments arranged by size:
1450 923 792 778 252 190 184 125
Bsp1286I G_dGCh'C
Cuts at: 154 224 249 1147 1331 1583 1708 2319 2412
Size: 70 25 898 184 252 125 611 93
Cuts at: 2412 2486 2676 2738 3468 154
Size: 74 190 62 730 1380
Fragments arranged by size:
1380 898 730 611 252 190 184 125
93 74 70 62 25
BspEI T'CCGG_A
Cuts at: 3914 3914
Size: 4694
BspHI T'CATG_A
Cuts at: 864 3870 864
Size: 3006 1688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 363 1744 1958 2260 2641 363
Size: 1381 214 302 381 2416
Fragments arranged by size:
2416 1381 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2986 3040 4524 4689 50
Size: 2936 54 1484 165 55
Fragments arranged by size:
2936 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1038 2606 1038
Size: 1568 3126
BsrFI r'CCGG_y
Cuts at: 2692 2873 3082 2692
Size: 181 209 4304
Fragments arranged by size:
4304 209 181
BsrGI T'GTAC_A
Cuts at: 990 1242 990
Size: 252 4442
BssSI C'ACGA_G
Cuts at: 2965 3323 2965
Size: 358 4336
BstAPI GCAn_nnn'nTGC
Cuts at: 358 2071 358
Size: 1713 2981
BstBI TT'CG_AA
Cuts at: 3055 3055
Size: 4694
BstYI r'GATC_y
Cuts at: 1 1169 1434 1719 2186 2209 2544 2790 3802
Size: 1168 265 285 467 23 335 246 1012
Cuts at: 3802 3888 3900 4246 1
Size: 86 12 346 449
Fragments arranged by size:
1168 1012 467 449 346 335 285 265
246 86 23 12
BtgI C'CryG_G
Cuts at: 131 145 131
Size: 14 4680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2644 2825 2988 2644
Size: 181 163 4350
Fragments arranged by size:
4350 181 163
BtsI GCAGTG_nn'
Cuts at: 1343 1937 1343
Size: 594 4100
DraI TTT'AAA
Cuts at: 1693 1693
Size: 4694
DrdI GACnn_nn'nnGTC
Cuts at: 2400 3258 2400
Size: 858 3836
EaeI y'GGCC_r
Cuts at: 398 2279 2453 2844 2871 4216 398
Size: 1881 174 391 27 1345 876
Fragments arranged by size:
1881 1345 876 391 174 27
EagI C'GGCC_G
Cuts at: 2279 2279
Size: 4694
EarI CTCTTCn'nnn_
Cuts at: 281 671 2717 2927 281
Size: 390 2046 210 2048
Fragments arranged by size:
2048 2046 390 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2902 3212 3358 4504 2902
Size: 310 146 1146 3092
Fragments arranged by size:
3092 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1145 1706 1145
Size: 561 4133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 436 725 863 1371 1539 2519 2951 3698
Size: 189 289 138 508 168 980 432 747
Cuts at: 3698 3966 247
Size: 268 975
Fragments arranged by size:
980 975 747 508 432 289 268 189
168 138
EcoNI CCTnn'n_nnAGG
Cuts at: 560 560
Size: 4694
EcoO109I rG'GnC_Cy
Cuts at: 150 246 553 1833 150
Size: 96 307 1280 3011
Fragments arranged by size:
3011 1280 307 96
EcoRI G'AATT_C
Cuts at: 1337 1421 1505 1698 1337
Size: 84 84 193 4333
Fragments arranged by size:
4333 193 84 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 4694
FspI TGC'GCA
Cuts at: 2475 2475
Size: 4694
HaeII r_GCGC'y
Cuts at: 734 2128 2376 3398 4377 734
Size: 1394 248 1022 979 1051
Fragments arranged by size:
1394 1051 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 966 978 998 1010 1218 1250 2536 2566 2568
Size: 12 20 12 208 32 1286 30 2
Cuts at: 2568 2598 966
Size: 30 3062
Fragments arranged by size:
3062 1286 208 32 30 30 20 12
12 2
HincII GTy'rAC
Cuts at: 1733 1733
Size: 4694
HindIII A'AGCT_T
Cuts at: 569 666 569
Size: 97 4597
KasI G'GCGC_C
Cuts at: 2124 2372 2124
Size: 248 4446
KpnI G_GTAC'C
Cuts at: 1714 1714
Size: 4694
MluI A'CGCG_T
Cuts at: 4127 4127
Size: 4694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1670 3365 3549 1670
Size: 1695 184 2815
Fragments arranged by size:
2815 1695 184
MscI TGG'CCA
Cuts at: 400 2455 400
Size: 2055 2639
MslI CAynn'nnrTG
Cuts at: 869 1262 1278 2683 2810 869
Size: 393 16 1405 127 2753
Fragments arranged by size:
2753 1405 393 127 16
MspA1I CmG'CkG
Cuts at: 1805 2119 2479 3492 3737 1805
Size: 314 360 1013 245 2762
Fragments arranged by size:
2762 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2875 2875
Size: 4694
NarI GG'CG_CC
Cuts at: 2125 2373 2125
Size: 248 4446
NcoI C'CATG_G
Cuts at: 131 145 131
Size: 14 4680
NdeI CA'TA_TG
Cuts at: 1275 1275
Size: 4694
NgoMIV G'CCGG_C
Cuts at: 2873 2873
Size: 4694
NspI r_CATG'y
Cuts at: 591 1478 1535 1747 2247 3154 591
Size: 887 57 212 500 907 2131
Fragments arranged by size:
2131 907 887 500 212 57
PciI A'CATG_T
Cuts at: 587 1474 1531 3150 587
Size: 887 57 1619 2131
Fragments arranged by size:
2131 1619 887 57
PmeI GTTT'AAAC
Cuts at: 1693 1693
Size: 4694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 3833
Size: 32 4662
PpuMI rG'GwC_Cy
Cuts at: 553 553
Size: 4694
PshAI GACnn'nnGTC
Cuts at: 1563 1563
Size: 4694
PspOMI G'GGCC_C
Cuts at: 150 245 150
Size: 95 4599
PstI C_TGCA'G
Cuts at: 117 1350 1369 1741 2426 117
Size: 1233 19 372 685 2385
Fragments arranged by size:
2385 1233 685 372 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4694
PvuII CAG'CTG
Cuts at: 2119 2479 2119
Size: 360 4334
RsrII CG'GwC_CG
Cuts at: 2889 2889
Size: 4694
SacI G_AGCT'C
Cuts at: 1147 1708 1147
Size: 561 4133
SalI G'TCGA_C
Cuts at: 1731 1731
Size: 4694
SapI GCTCTTCn'nnn_
Cuts at: 2717 2927 2717
Size: 210 4484
SbfI CC_TGCA'GG
Cuts at: 1741 1741
Size: 4694
ScaI AGT'ACT
Cuts at: 4211 4211
Size: 4694
SfcI C'TryA_G
Cuts at: 33 113 463 1346 1365 1737 2422 3415 3606
Size: 80 350 883 19 372 685 993 191
Cuts at: 3606 33
Size: 1121
Fragments arranged by size:
1121 993 883 685 372 350 191 80
19
SfoI GGC'GCC
Cuts at: 2126 2374 2126
Size: 248 4446
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4694
SmaI CCC'GGG
Cuts at: 1716 1716
Size: 4694
SmlI C'TyrA_G
Cuts at: 548 1849 2764 3256 3518 548
Size: 1301 915 492 262 1724
Fragments arranged by size:
1724 1301 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4694
SphI G_CATG'C
Cuts at: 1747 2247 1747
Size: 500 4194
StuI AGG'CCT
Cuts at: 272 880 1468 1552 272
Size: 608 588 84 3414
Fragments arranged by size:
3414 608 588 84
StyI C'CwwG_G
Cuts at: 131 145 369 1828 2182 131
Size: 14 224 1459 354 2643
Fragments arranged by size:
2643 1459 354 224 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2277 2410 2869 3078 2277
Size: 133 459 209 3893
Fragments arranged by size:
3893 459 209 133
TatI w'GTAC_w
Cuts at: 990 1242 4209 990
Size: 252 2967 1475
Fragments arranged by size:
2967 1475 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4211 4243 4211
Size: 32 4662
Tth111I GACn'n_nGTC
Cuts at: 2491 2491
Size: 4694
XbaI T'CTAG_A
Cuts at: 1725 1725
Size: 4694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2189 2189
Size: 4694
XmaI C'CCGG_G
Cuts at: 1714 1714
Size: 4694
XmnI GAAnn'nnTTC
Cuts at: 625 880 964 1384 1468 1552 625
Size: 255 84 420 84 84 3767
Fragments arranged by size:
3767 420 255 84 84 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PmeI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI