Restriction map for
pF1KB7811
(Circular) (Six-base) MAPSORT of: pF1KB7811.seq Check: 1262 from: 1 to: 4700
pF1KB7811 4700 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1738 1738
Size: 4700
Acc65I G'GTAC_C
Cuts at: 1716 1716
Size: 4700
AclI AA'CG_TT
Cuts at: 4540 4540
Size: 4700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 1311 2525 2957 3704 3972 502
Size: 809 1214 432 747 268 1230
Fragments arranged by size:
1230 1214 809 747 432 268
AflIII A'CryG_T
Cuts at: 242 329 2052 3156 4133 242
Size: 87 1723 1104 977 809
Fragments arranged by size:
1723 1104 977 809 87
AgeI A'CCGG_T
Cuts at: 3088 3088
Size: 4700
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 659 1585 659
Size: 926 3774
AlwNI CAG_nnn'CTG
Cuts at: 684 1524 2005 3572 684
Size: 840 481 1567 1812
Fragments arranged by size:
1812 1567 840 481
ApaI G_GGCC'C
Cuts at: 127 143 823 127
Size: 16 680 4004
Fragments arranged by size:
4004 680 16
ApaLI G'TGCA_C
Cuts at: 1504 3470 1504
Size: 1966 2734
ApoI r'AATT_y
Cuts at: 1704 4053 4309 1704
Size: 2349 256 2095
Fragments arranged by size:
2349 2095 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4700
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
AvaI C'yCGr_G
Cuts at: 222 816 1031 1051 1462 1720 222
Size: 594 215 20 411 258 3202
Fragments arranged by size:
3202 594 411 258 215 20
BamHI G'GATC_C
Cuts at: 710 1725 710
Size: 1015 3685
BanI G'GyrC_C
Cuts at: 1055 1716 2130 2378 2413 1055
Size: 661 414 248 35 3342
Fragments arranged by size:
3342 661 414 248 35
BanII G_rGCy'C
Cuts at: 127 143 223 823 1010 1030 1259 1433 1451
Size: 16 80 600 187 20 229 174 18
Cuts at: 1451 1600 1714 2744 127
Size: 149 114 1030 2083
Fragments arranged by size:
2083 1030 600 229 187 174 149 114
80 20 18 16
BbeI G_GCGC'C
Cuts at: 1059 2134 2382 1059
Size: 1075 248 3377
Fragments arranged by size:
3377 1075 248
BbsI GAAGACnn'nnnn_
Cuts at: 497 497
Size: 4700
BbvCI CC'TCA_GC
Cuts at: 1228 1228
Size: 4700
BciVI GTATCCnnnnn_n'
Cuts at: 1432 2598 3359 4700 1432
Size: 1166 761 1341 1432
Fragments arranged by size:
1432 1341 1166 761
BclI T'GATC_A
Cuts at: 2220 2220
Size: 4700
BglI GCCn_nnn'nGGC
Cuts at: 1455 1455
Size: 4700
BglII A'GATC_T
Cuts at: 2215 4252 2215
Size: 2037 2663
BlpI GC'TnA_GC
Cuts at: 759 1312 1812 759
Size: 553 500 3647
Fragments arranged by size:
3647 553 500
Bme1580I G_kGCm'C
Cuts at: 127 143 823 1347 1382 1508 2325 2418 3474
Size: 16 680 524 35 126 817 93 1056
Cuts at: 3474 127
Size: 1353
Fragments arranged by size:
1353 1056 817 680 524 126 93 35
16
BmgBI CAC'GTC
Cuts at: 537 807 537
Size: 270 4430
BmrI ACTGGGnnnn_n'
Cuts at: 1912 1993 2328 4472 1912
Size: 81 335 2144 2140
Fragments arranged by size:
2144 2140 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 4700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 820 840 237
Size: 583 20 4097
Fragments arranged by size:
4097 583 20
Bpu10I CC'TnA_GC
Cuts at: 1228 1228
Size: 4700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 1876 2755 3247 3545 463
Size: 1413 879 492 298 1618
Fragments arranged by size:
1618 1413 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4404 4404
Size: 4700
BsaAI yAC'GTr
Cuts at: 243 2053 243
Size: 1810 2890
BsaBI GATnn'nnATC
Cuts at: 6 2214 4251 6
Size: 2208 2037 455
Fragments arranged by size:
2208 2037 455
BsaHI Gr'CG_yC
Cuts at: 1056 1157 2131 2379 3081 4577 1056
Size: 101 974 248 702 1496 1179
Fragments arranged by size:
1496 1179 974 702 248 101
BsaWI w'CCGG_w
Cuts at: 341 707 1889 2410 3088 3362 3509 3920 341
Size: 366 1182 521 678 274 147 411 1121
Fragments arranged by size:
1182 1121 678 521 411 366 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 868 1528 1558 838
Size: 30 660 30 3980
Fragments arranged by size:
3980 660 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1018 1054 1018
Size: 36 4664
BseYI C'CCAG_C
Cuts at: 142 647 840 1012 1587 2125 2883 3460 3956
Size: 505 193 172 575 538 758 577 496
Cuts at: 3956 142
Size: 886
Fragments arranged by size:
886 758 577 575 538 505 496 193
172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 718 559
Size: 159 4541
BsiEI CG_ry'CG
Cuts at: 104 1091 2242 2288 3496 104
Size: 987 1151 46 1208 1308
Fragments arranged by size:
1308 1208 1151 987 46
BsiHKAI G_wGCw'C
Cuts at: 806 1259 1433 1508 1714 2492 2682 3474 806
Size: 453 174 75 206 778 190 792 2032
Fragments arranged by size:
2032 792 778 453 206 190 174 75
BsmBI CGTCTCn'nnnn_
Cuts at: 383 383
Size: 4700
Bsp1286I G_dGCh'C
Cuts at: 127 143 223 806 823 1010 1030 1259 1347
Size: 16 80 583 17 187 20 229 88
Cuts at: 1347 1382 1433 1451 1508 1600 1714 2325 2418
Size: 35 51 18 57 92 114 611 93
Cuts at: 2418 2492 2682 2744 3474 127
Size: 74 190 62 730 1353
Fragments arranged by size:
1353 730 611 583 229 190 187 114
93 92 88 80 74 62 57 51
35 20 18 17 16
BspEI T'CCGG_A
Cuts at: 341 3920 341
Size: 3579 1121
BspHI T'CATG_A
Cuts at: 3876 3876
Size: 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1134 1307 1577 1750 1964 2266 2647 1134
Size: 173 270 173 214 302 381 3187
Fragments arranged by size:
3187 381 302 270 214 173 173
BsrBI CCG'CTC
Cuts at: 50 227 2992 3046 4530 4695 50
Size: 177 2765 54 1484 165 55
Fragments arranged by size:
2765 1484 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2612 2612
Size: 4700
BsrFI r'CCGG_y
Cuts at: 785 1184 1572 2698 2879 3088 785
Size: 399 388 1126 181 209 2397
Fragments arranged by size:
2397 1126 399 388 209 181
BssSI C'ACGA_G
Cuts at: 181 2971 3329 181
Size: 2790 358 1552
Fragments arranged by size:
2790 1552 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1308 1503 2077 1308
Size: 195 574 3931
Fragments arranged by size:
3931 574 195
BstBI TT'CG_AA
Cuts at: 3061 3061
Size: 4700
BstEII G'GTnAC_C
Cuts at: 1371 1371
Size: 4700
BstXI CCAn_nnnn'nTGG
Cuts at: 582 936 582
Size: 354 4346
BstYI r'GATC_y
Cuts at: 1 111 500 710 731 1725 2192 2215 2550
Size: 110 389 210 21 994 467 23 335
Cuts at: 2550 2796 3808 3894 3906 4252 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 994 467 449 389 346 335 246
210 110 86 23 21 12
BtgI C'CryG_G
Cuts at: 107 119 810 935 1067 1320 1547 107
Size: 12 691 125 132 253 227 3260
Fragments arranged by size:
3260 691 253 227 132 125 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2650 2831 2994 2650
Size: 181 163 4356
Fragments arranged by size:
4356 181 163
BtsI GCAGTG_nn'
Cuts at: 1943 1943
Size: 4700
DraI TTT'AAA
Cuts at: 1699 1699
Size: 4700
DrdI GACnn_nn'nnGTC
Cuts at: 471 1687 2406 3264 471
Size: 1216 719 858 1907
Fragments arranged by size:
1907 1216 858 719
EaeI y'GGCC_r
Cuts at: 305 426 584 623 881 1415 2285 2459 2850
Size: 121 158 39 258 534 870 174 391
Cuts at: 2850 2877 4222 305
Size: 27 1345 783
Fragments arranged by size:
1345 870 783 534 391 258 174 158
121 39 27
EagI C'GGCC_G
Cuts at: 2285 2285
Size: 4700
EarI CTCTTCn'nnn_
Cuts at: 478 718 2723 2933 478
Size: 240 2005 210 2245
Fragments arranged by size:
2245 2005 240 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 493 2908 3218 3364 4510 493
Size: 2415 310 146 1146 683
Fragments arranged by size:
2415 1146 683 310 146
EcoICRI GAG'CTC
Cuts at: 1257 1431 1712 1257
Size: 174 281 4245
Fragments arranged by size:
4245 281 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 502 820 840 1311 2525 2957 3704 3972
Size: 265 318 20 471 1214 432 747 268
Cuts at: 3972 237
Size: 965
Fragments arranged by size:
1214 965 747 471 432 318 268 265
20
EcoO109I rG'GnC_Cy
Cuts at: 124 139 820 968 1632 1680 1839 124
Size: 15 681 148 664 48 159 2985
Fragments arranged by size:
2985 681 664 159 148 48 15
EcoRI G'AATT_C
Cuts at: 1704 1704
Size: 4700
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4700
FseI GG_CCGG'CC
Cuts at: 1188 1188
Size: 4700
FspI TGC'GCA
Cuts at: 2481 2481
Size: 4700
HaeII r_GCGC'y
Cuts at: 1059 2134 2382 3404 4383 1059
Size: 1075 248 1022 979 1376
Fragments arranged by size:
1376 1075 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 742 774 884 916 1420 1452 1528 1560 2542
Size: 32 110 32 504 32 76 32 982
Cuts at: 2542 2572 2574 2604 742
Size: 30 2 30 2838
Fragments arranged by size:
2838 982 504 110 76 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 666 1739 666
Size: 1073 3627
KasI G'GCGC_C
Cuts at: 1055 2130 2378 1055
Size: 1075 248 3377
Fragments arranged by size:
3377 1075 248
KpnI G_GTAC'C
Cuts at: 1720 1720
Size: 4700
MluI A'CGCG_T
Cuts at: 4133 4133
Size: 4700
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 929 1310 3371 3555 929
Size: 381 2061 184 2074
Fragments arranged by size:
2074 2061 381 184
MscI TGG'CCA
Cuts at: 307 428 586 625 883 1417 2461 307
Size: 121 158 39 258 534 1044 2546
Fragments arranged by size:
2546 1044 534 258 158 121 39
MslI CAynn'nnrTG
Cuts at: 599 1271 2689 2816 599
Size: 672 1418 127 2483
Fragments arranged by size:
2483 1418 672 127
MspA1I CmG'CkG
Cuts at: 543 1500 1524 1811 2125 2485 3498 3743 543
Size: 957 24 287 314 360 1013 245 1500
Fragments arranged by size:
1500 1013 957 360 314 287 245 24
NaeI GCC'GGC
Cuts at: 787 1186 2881 787
Size: 399 1695 2606
Fragments arranged by size:
2606 1695 399
NarI GG'CG_CC
Cuts at: 1056 2131 2379 1056
Size: 1075 248 3377
Fragments arranged by size:
3377 1075 248
NcoI C'CATG_G
Cuts at: 107 935 107
Size: 828 3872
NgoMIV G'CCGG_C
Cuts at: 785 1184 2879 785
Size: 399 1695 2606
Fragments arranged by size:
2606 1695 399
NspI r_CATG'y
Cuts at: 333 646 1753 2253 3160 333
Size: 313 1107 500 907 1873
Fragments arranged by size:
1873 1107 907 500 313
PciI A'CATG_T
Cuts at: 329 3156 329
Size: 2827 1873
PflMI CCAn_nnn'nTGG
Cuts at: 173 173
Size: 4700
PmeI GTTT'AAAC
Cuts at: 1699 1699
Size: 4700
PmlI CAC'GTG
Cuts at: 243 243
Size: 4700
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4668
PpuMI rG'GwC_Cy
Cuts at: 1632 1680 1632
Size: 48 4652
PspOMI G'GGCC_C
Cuts at: 123 139 819 123
Size: 16 680 4004
Fragments arranged by size:
4004 680 16
PstI C_TGCA'G
Cuts at: 1304 1747 2432 1304
Size: 443 685 3572
Fragments arranged by size:
3572 685 443
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4700
PvuII CAG'CTG
Cuts at: 543 1500 2125 2485 543
Size: 957 625 360 2758
Fragments arranged by size:
2758 957 625 360
RsrII CG'GwC_CG
Cuts at: 2895 2895
Size: 4700
SacI G_AGCT'C
Cuts at: 1259 1433 1714 1259
Size: 174 281 4245
Fragments arranged by size:
4245 281 174
SalI G'TCGA_C
Cuts at: 1737 1737
Size: 4700
SapI GCTCTTCn'nnn_
Cuts at: 2723 2933 2723
Size: 210 4490
SbfI CC_TGCA'GG
Cuts at: 1747 1747
Size: 4700
ScaI AGT'ACT
Cuts at: 286 4217 286
Size: 3931 769
SfcI C'TryA_G
Cuts at: 33 846 1300 1743 2428 3421 3612 33
Size: 813 454 443 685 993 191 1121
Fragments arranged by size:
1121 993 813 685 454 443 191
SfoI GGC'GCC
Cuts at: 1057 2132 2380 1057
Size: 1075 248 3377
Fragments arranged by size:
3377 1075 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4700
SmaI CCC'GGG
Cuts at: 1033 1053 1722 1033
Size: 20 669 4011
Fragments arranged by size:
4011 669 20
SmlI C'TyrA_G
Cuts at: 442 1855 2770 3262 3524 442
Size: 1413 915 492 262 1618
Fragments arranged by size:
1618 1413 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4700
SphI G_CATG'C
Cuts at: 646 1753 2253 646
Size: 1107 500 3093
Fragments arranged by size:
3093 1107 500
StuI AGG'CCT
Cuts at: 1458 1458
Size: 4700
StyI C'CwwG_G
Cuts at: 107 266 935 1676 1834 2188 107
Size: 159 669 741 158 354 2619
Fragments arranged by size:
2619 741 669 354 159 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 425 2283 2416 2875 3084 425
Size: 1858 133 459 209 2041
Fragments arranged by size:
2041 1858 459 209 133
TatI w'GTAC_w
Cuts at: 284 4215 284
Size: 3931 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4217 4249 4217
Size: 32 4668
Tth111I GACn'n_nGTC
Cuts at: 514 2497 514
Size: 1983 2717
XbaI T'CTAG_A
Cuts at: 1731 1731
Size: 4700
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 984 1347 2195 984
Size: 363 848 3489
Fragments arranged by size:
3489 848 363
XmaI C'CCGG_G
Cuts at: 1031 1051 1720 1031
Size: 20 669 4011
Fragments arranged by size:
4011 669 20
XmnI GAAnn'nnTTC
Cuts at: 235 235
Size: 4700
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI