Restriction map for
pF1KB7813
(Circular) (Six-base) MAPSORT of: pF1KB7813.seq Check: 5604 from: 1 to: 4709
pF1KB7813 4709 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AatII G_ACGT'C
Cuts at: 473 473
Size: 4709
AccI GT'mk_AC
Cuts at: 1299 1747 1299
Size: 448 4261
Acc65I G'GTAC_C
Cuts at: 1725 1725
Size: 4709
AclI AA'CG_TT
Cuts at: 4549 4549
Size: 4709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1181 1507 2534 2966 3713 3981 1181
Size: 326 1027 432 747 268 1909
Fragments arranged by size:
1909 1027 747 432 326 268
AflIII A'CryG_T
Cuts at: 157 2061 3165 4142 157
Size: 1904 1104 977 724
Fragments arranged by size:
1904 1104 977 724
AgeI A'CCGG_T
Cuts at: 3097 3097
Size: 4709
AhdI GACnn_n'nnGTC
Cuts at: 916 1377 916
Size: 461 4248
AleI CACnn'nnGTG
Cuts at: 530 530
Size: 4709
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 527 559 527
Size: 32 4677
AlwNI CAG_nnn'CTG
Cuts at: 2014 3581 2014
Size: 1567 3142
ApaI G_GGCC'C
Cuts at: 497 497
Size: 4709
ApaLI G'TGCA_C
Cuts at: 3479 3479
Size: 4709
ApoI r'AATT_y
Cuts at: 267 1713 4062 4318 267
Size: 1446 2349 256 658
Fragments arranged by size:
2349 1446 658 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4709
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
AvaI C'yCGr_G
Cuts at: 1729 1729
Size: 4709
BamHI G'GATC_C
Cuts at: 1734 1734
Size: 4709
BanI G'GyrC_C
Cuts at: 897 1725 2139 2387 2422 897
Size: 828 414 248 35 3184
Fragments arranged by size:
3184 828 414 248 35
BanII G_rGCy'C
Cuts at: 497 1483 1723 2753 497
Size: 986 240 1030 2453
Fragments arranged by size:
2453 1030 986 240
BbeI G_GCGC'C
Cuts at: 2143 2391 2143
Size: 248 4461
BbsI GAAGACnn'nnnn_
Cuts at: 1497 1497
Size: 4709
BbvCI CC'TCA_GC
Cuts at: 1115 1115
Size: 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4675
BciVI GTATCCnnnnn_n'
Cuts at: 1452 2607 3368 4709 1452
Size: 1155 761 1341 1452
Fragments arranged by size:
1452 1341 1155 761
BclI T'GATC_A
Cuts at: 2229 2229
Size: 4709
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4709
BglII A'GATC_T
Cuts at: 2224 4261 2224
Size: 2037 2672
BlpI GC'TnA_GC
Cuts at: 1052 1182 1821 1052
Size: 130 639 3940
Fragments arranged by size:
3940 639 130
Bme1580I G_kGCm'C
Cuts at: 497 2334 2427 3483 497
Size: 1837 93 1056 1723
Fragments arranged by size:
1837 1723 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1165 1921 2002 2337 4481 1165
Size: 756 81 335 2144 1393
Fragments arranged by size:
2144 1393 756 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 4709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 775 778 635
Size: 140 3 4566
Fragments arranged by size:
4566 140 3
Bpu10I CC'TnA_GC
Cuts at: 319 1115 319
Size: 796 3913
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 201 1885 2764 3256 3554 201
Size: 1684 879 492 298 1356
Fragments arranged by size:
1684 1356 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 903 4413 903
Size: 3510 1199
BsaAI yAC'GTr
Cuts at: 2062 2062
Size: 4709
BsaBI GATnn'nnATC
Cuts at: 6 2223 4260 6
Size: 2217 2037 455
Fragments arranged by size:
2217 2037 455
BsaHI Gr'CG_yC
Cuts at: 470 668 2140 2388 3090 4586 470
Size: 198 1472 248 702 1496 593
Fragments arranged by size:
1496 1472 702 593 248 198
BsaWI w'CCGG_w
Cuts at: 1898 2419 3097 3371 3518 3929 1898
Size: 521 678 274 147 411 2678
Fragments arranged by size:
2678 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1455 1485 1455
Size: 30 4679
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 775 775
Size: 4709
BseYI C'CCAG_C
Cuts at: 814 1324 1425 2134 2892 3469 3965 814
Size: 510 101 709 758 577 496 1558
Fragments arranged by size:
1558 758 709 577 510 496 101
BsiEI CG_ry'CG
Cuts at: 104 2251 2297 3505 104
Size: 2147 46 1208 1308
Fragments arranged by size:
2147 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 767 1550 1694 1723 2501 2691 3483 767
Size: 783 144 29 778 190 792 1993
Fragments arranged by size:
1993 792 783 778 190 144 29
BsmI GAATG_Cn'
Cuts at: 1630 1630
Size: 4709
Bsp1286I G_dGCh'C
Cuts at: 497 767 1483 1550 1694 1723 2334 2427 2501
Size: 270 716 67 144 29 611 93 74
Cuts at: 2501 2691 2753 3483 497
Size: 190 62 730 1723
Fragments arranged by size:
1723 730 716 611 270 190 144 93
74 67 62 29
BspEI T'CCGG_A
Cuts at: 3929 3929
Size: 4709
BspHI T'CATG_A
Cuts at: 824 1251 3885 824
Size: 427 2634 1648
Fragments arranged by size:
2634 1648 427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 810 921 1759 1973 2275 2656 810
Size: 111 838 214 302 381 2863
Fragments arranged by size:
2863 838 381 302 214 111
BsrBI CCG'CTC
Cuts at: 50 3001 3055 4539 4704 50
Size: 2951 54 1484 165 55
Fragments arranged by size:
2951 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 111 2621 111
Size: 2510 2199
BsrFI r'CCGG_y
Cuts at: 2707 2888 3097 2707
Size: 181 209 4319
Fragments arranged by size:
4319 209 181
BsrGI T'GTAC_A
Cuts at: 1580 1580
Size: 4709
BssSI C'ACGA_G
Cuts at: 2980 3338 2980
Size: 358 4351
BstAPI GCAn_nnn'nTGC
Cuts at: 485 1334 2086 485
Size: 849 752 3108
Fragments arranged by size:
3108 849 752
BstBI TT'CG_AA
Cuts at: 3070 3070
Size: 4709
BstXI CCAn_nnnn'nTGG
Cuts at: 1593 1593
Size: 4709
BstYI r'GATC_y
Cuts at: 1 1734 2201 2224 2559 2805 3817 3903 3915
Size: 1733 467 23 335 246 1012 86 12
Cuts at: 3915 4261 1
Size: 346 449
Fragments arranged by size:
1733 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 685 1475 685
Size: 790 3919
BtgI C'CryG_G
Cuts at: 1602 1602
Size: 4709
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2659 2840 3003 2659
Size: 181 163 4365
Fragments arranged by size:
4365 181 163
BtsI GCAGTG_nn'
Cuts at: 1548 1952 1548
Size: 404 4305
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 708 743 1617 1652 708
Size: 35 874 35 3765
Fragments arranged by size:
3765 874 35 35
DraI TTT'AAA
Cuts at: 1708 1708
Size: 4709
DrdI GACnn_nn'nnGTC
Cuts at: 1295 2415 3273 1295
Size: 1120 858 2731
Fragments arranged by size:
2731 1120 858
EaeI y'GGCC_r
Cuts at: 2294 2468 2859 2886 4231 2294
Size: 174 391 27 1345 2772
Fragments arranged by size:
2772 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2294 2294
Size: 4709
EarI CTCTTCn'nnn_
Cuts at: 293 2732 2942 293
Size: 2439 210 2060
Fragments arranged by size:
2439 2060 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2917 3227 3373 4519 2917
Size: 310 146 1146 3107
Fragments arranged by size:
3107 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1721 1721
Size: 4709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 635 775 778 1181 1507 2534 2966 3713 3981
Size: 140 3 403 326 1027 432 747 268
Cuts at: 3981 635
Size: 1363
Fragments arranged by size:
1363 1027 747 432 403 326 268 140
3
EcoO109I rG'GnC_Cy
Cuts at: 689 1119 1532 1614 1848 689
Size: 430 413 82 234 3550
Fragments arranged by size:
3550 430 413 234 82
EcoRI G'AATT_C
Cuts at: 1713 1713
Size: 4709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1202 1445 2728 1202
Size: 243 1283 3183
Fragments arranged by size:
3183 1283 243
FspI TGC'GCA
Cuts at: 2490 2490
Size: 4709
HaeII r_GCGC'y
Cuts at: 2143 2391 3413 4392 2143
Size: 248 1022 979 2460
Fragments arranged by size:
2460 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 902 934 1050 1082 1363 1395 1654
Size: 32 671 32 116 32 281 32 259
Cuts at: 1654 1686 2551 2581 2583 2613 199
Size: 32 865 30 2 30 2295
Fragments arranged by size:
2295 865 671 281 259 116 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1300 1748 1300
Size: 448 4261
HindIII A'AGCT_T
Cuts at: 1451 1451
Size: 4709
KasI G'GCGC_C
Cuts at: 2139 2387 2139
Size: 248 4461
KpnI G_GTAC'C
Cuts at: 1729 1729
Size: 4709
MfeI C'AATT_G
Cuts at: 203 203
Size: 4709
MluI A'CGCG_T
Cuts at: 4142 4142
Size: 4709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3380 3564 3380
Size: 184 4525
MscI TGG'CCA
Cuts at: 2470 2470
Size: 4709
MslI CAynn'nnrTG
Cuts at: 530 1591 1623 2698 2825 530
Size: 1061 32 1075 127 2414
Fragments arranged by size:
2414 1075 1061 127 32
MspA1I CmG'CkG
Cuts at: 1820 2134 2494 3507 3752 1820
Size: 314 360 1013 245 2777
Fragments arranged by size:
2777 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2890 2890
Size: 4709
NarI GG'CG_CC
Cuts at: 2140 2388 2140
Size: 248 4461
NcoI C'CATG_G
Cuts at: 1602 1602
Size: 4709
NdeI CA'TA_TG
Cuts at: 1038 1070 1038
Size: 32 4677
NgoMIV G'CCGG_C
Cuts at: 2888 2888
Size: 4709
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4709
NspI r_CATG'y
Cuts at: 241 1762 2262 3169 241
Size: 1521 500 907 1781
Fragments arranged by size:
1781 1521 907 500
PciI A'CATG_T
Cuts at: 3165 3165
Size: 4709
PflMI CCAn_nnn'nTGG
Cuts at: 699 699
Size: 4709
PfoI T'CCnGG_A
Cuts at: 413 413
Size: 4709
PmeI GTTT'AAAC
Cuts at: 1708 1708
Size: 4709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 610 642 3848 3880 610
Size: 32 3206 32 1439
Fragments arranged by size:
3206 1439 32 32
PpuMI rG'GwC_Cy
Cuts at: 689 1614 689
Size: 925 3784
PsiI TTA'TAA
Cuts at: 1177 1177
Size: 4709
PspOMI G'GGCC_C
Cuts at: 493 493
Size: 4709
PstI C_TGCA'G
Cuts at: 1756 2441 1756
Size: 685 4024
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4709
PvuII CAG'CTG
Cuts at: 2134 2494 2134
Size: 360 4349
RsrII CG'GwC_CG
Cuts at: 2904 2904
Size: 4709
SacI G_AGCT'C
Cuts at: 1723 1723
Size: 4709
SalI G'TCGA_C
Cuts at: 1298 1746 1298
Size: 448 4261
SanDI GG'GwC_CC
Cuts at: 1614 1614
Size: 4709
SapI GCTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
SbfI CC_TGCA'GG
Cuts at: 1756 1756
Size: 4709
ScaI AGT'ACT
Cuts at: 4226 4226
Size: 4709
SfcI C'TryA_G
Cuts at: 33 642 654 1641 1752 2437 3430 3621 33
Size: 609 12 987 111 685 993 191 1121
Fragments arranged by size:
1121 993 987 685 609 191 111 12
SfoI GGC'GCC
Cuts at: 2141 2389 2141
Size: 248 4461
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
SmaI CCC'GGG
Cuts at: 1731 1731
Size: 4709
SmlI C'TyrA_G
Cuts at: 216 1864 2779 3271 3533 216
Size: 1648 915 492 262 1392
Fragments arranged by size:
1648 1392 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4709
SphI G_CATG'C
Cuts at: 241 1762 2262 241
Size: 1521 500 2688
Fragments arranged by size:
2688 1521 500
SspI AAT'ATT
Cuts at: 888 888
Size: 4709
StuI AGG'CCT
Cuts at: 1318 1318
Size: 4709
StyI C'CwwG_G
Cuts at: 1107 1386 1466 1602 1843 2197 1107
Size: 279 80 136 241 354 3619
Fragments arranged by size:
3619 354 279 241 136 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 2292 2425 2884 3093 240
Size: 2052 133 459 209 1856
Fragments arranged by size:
2052 1856 459 209 133
TatI w'GTAC_w
Cuts at: 1580 4224 1580
Size: 2644 2065
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4226 4258 4226
Size: 32 4677
Tth111I GACn'n_nGTC
Cuts at: 1573 2506 1573
Size: 933 3776
XbaI T'CTAG_A
Cuts at: 1740 1740
Size: 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 504 2204 504
Size: 1700 3009
XmaI C'CCGG_G
Cuts at: 1729 1729
Size: 4709
XmnI GAAnn'nnTTC
Cuts at: 625 625
Size: 4709
ZraI GAC'GTC
Cuts at: 471 471
Size: 4709
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AscI AvrII BaeI BglI
BmgBI BmtI BsgI BsiWI BsmBI BssHII BstEII BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HpaI NheI
NotI NruI PacI PasI PmlI PshAI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI