Restriction map for
pF1KB7814
(Circular) (Six-base) MAPSORT of: pF1KB7814.seq Check: 4098 from: 1 to: 4874
pF1KB7814 4874 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1737 1737
Size: 4874
AatII G_ACGT'C
Cuts at: 1435 1435
Size: 4874
AccI GT'mk_AC
Cuts at: 527 1112 1912 527
Size: 585 800 3489
Fragments arranged by size:
3489 800 585
Acc65I G'GTAC_C
Cuts at: 1890 1890
Size: 4874
AclI AA'CG_TT
Cuts at: 4714 4714
Size: 4874
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1383 2699 3131 3878 4146 1240
Size: 143 1316 432 747 268 1968
Fragments arranged by size:
1968 1316 747 432 268 143
AflIII A'CryG_T
Cuts at: 800 1421 2226 3330 4307 800
Size: 621 805 1104 977 1367
Fragments arranged by size:
1367 1104 977 805 621
AgeI A'CCGG_T
Cuts at: 3262 3262
Size: 4874
AlwNI CAG_nnn'CTG
Cuts at: 646 2179 3746 646
Size: 1533 1567 1774
Fragments arranged by size:
1774 1567 1533
ApaI G_GGCC'C
Cuts at: 840 840
Size: 4874
ApaLI G'TGCA_C
Cuts at: 808 3644 808
Size: 2836 2038
ApoI r'AATT_y
Cuts at: 1878 4227 4483 1878
Size: 2349 256 2269
Fragments arranged by size:
2349 2269 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4874
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
AvaI C'yCGr_G
Cuts at: 722 1894 722
Size: 1172 3702
BamHI G'GATC_C
Cuts at: 1899 1899
Size: 4874
BanI G'GyrC_C
Cuts at: 913 1567 1890 2304 2552 2587 913
Size: 654 323 414 248 35 3200
Fragments arranged by size:
3200 654 414 323 248 35
BanII G_rGCy'C
Cuts at: 840 1888 2918 840
Size: 1048 1030 2796
Fragments arranged by size:
2796 1048 1030
BbeI G_GCGC'C
Cuts at: 1571 2308 2556 1571
Size: 737 248 3889
Fragments arranged by size:
3889 737 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1655 1689 1655
Size: 34 4840
BciVI GTATCCnnnnn_n'
Cuts at: 2772 3533 4874 2772
Size: 761 1341 2772
Fragments arranged by size:
2772 1341 761
BclI T'GATC_A
Cuts at: 2394 2394
Size: 4874
BglI GCCn_nnn'nGGC
Cuts at: 229 229
Size: 4874
BglII A'GATC_T
Cuts at: 480 2389 4426 480
Size: 1909 2037 928
Fragments arranged by size:
2037 1909 928
BlpI GC'TnA_GC
Cuts at: 1986 1986
Size: 4874
Bme1580I G_kGCm'C
Cuts at: 812 840 2499 2592 3648 812
Size: 28 1659 93 1056 2038
Fragments arranged by size:
2038 1659 1056 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 884 2086 2167 2502 4646 884
Size: 1202 81 335 2144 1112
Fragments arranged by size:
2144 1202 1112 335 81
BmtI G_CTAG'C
Cuts at: 1414 1414
Size: 4874
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3241 3241
Size: 4874
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 427
Size: 4874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 2050 2929 3421 3719 667
Size: 1383 879 492 298 1822
Fragments arranged by size:
1822 1383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4578 4578
Size: 4874
BsaAI yAC'GTr
Cuts at: 780 2227 780
Size: 1447 3427
BsaBI GATnn'nnATC
Cuts at: 6 2388 4425 6
Size: 2382 2037 455
Fragments arranged by size:
2382 2037 455
BsaHI Gr'CG_yC
Cuts at: 221 1046 1388 1432 1568 2305 2553 3255 4751
Size: 825 342 44 136 737 248 702 1496
Cuts at: 4751 221
Size: 344
Fragments arranged by size:
1496 825 737 702 344 342 248 136
44
BsaWI w'CCGG_w
Cuts at: 293 2063 2584 3262 3536 3683 4094 293
Size: 1770 521 678 274 147 411 1073
Fragments arranged by size:
1770 1073 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 1608 1787 1790 172
Size: 1436 179 3 3256
Fragments arranged by size:
3256 1436 179 3
BseYI C'CCAG_C
Cuts at: 165 1501 1514 2299 3057 3634 4130 165
Size: 1336 13 785 758 577 496 909
Fragments arranged by size:
1336 909 785 758 577 496 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 609 1352 1794 609
Size: 743 442 3689
Fragments arranged by size:
3689 743 442
BsiEI CG_ry'CG
Cuts at: 104 233 2416 2462 3670 104
Size: 129 2183 46 1208 1308
Fragments arranged by size:
2183 1308 1208 129 46
BsiHKAI G_wGCw'C
Cuts at: 812 1098 1888 2666 2856 3648 812
Size: 286 790 778 190 792 2038
Fragments arranged by size:
2038 792 790 778 286 190
BsmI GAATG_Cn'
Cuts at: 714 1262 714
Size: 548 4326
BsmBI CGTCTCn'nnnn_
Cuts at: 1423 1423
Size: 4874
Bsp1286I G_dGCh'C
Cuts at: 812 840 1098 1888 2499 2592 2666 2856 2918
Size: 28 258 790 611 93 74 190 62
Cuts at: 2918 3648 812
Size: 730 2038
Fragments arranged by size:
2038 790 730 611 258 190 93 74
62 28
BspEI T'CCGG_A
Cuts at: 293 4094 293
Size: 3801 1073
BspHI T'CATG_A
Cuts at: 4050 4050
Size: 4874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1737 1924 2138 2440 2821 1737
Size: 187 214 302 381 3790
Fragments arranged by size:
3790 381 302 214 187
BsrBI CCG'CTC
Cuts at: 50 242 1294 3166 3220 4704 4869 50
Size: 192 1052 1872 54 1484 165 55
Fragments arranged by size:
1872 1484 1052 192 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2786 2786
Size: 4874
BsrFI r'CCGG_y
Cuts at: 348 593 2872 3053 3262 348
Size: 245 2279 181 209 1960
Fragments arranged by size:
2279 1960 245 209 181
BsrGI T'GTAC_A
Cuts at: 452 1316 452
Size: 864 4010
BssSI C'ACGA_G
Cuts at: 192 577 3145 3503 192
Size: 385 2568 358 1563
Fragments arranged by size:
2568 1563 385 358
BstAPI GCAn_nnn'nTGC
Cuts at: 596 2251 596
Size: 1655 3219
BstBI TT'CG_AA
Cuts at: 3235 3235
Size: 4874
BstEII G'GTnAC_C
Cuts at: 1199 1677 1199
Size: 478 4396
BstYI r'GATC_y
Cuts at: 1 480 1343 1899 2366 2389 2724 2970 3982
Size: 479 863 556 467 23 335 246 1012
Cuts at: 3982 4068 4080 4426 1
Size: 86 12 346 449
Fragments arranged by size:
1012 863 556 479 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 905 1571 1661 1775 905
Size: 666 90 114 4004
Fragments arranged by size:
4004 666 114 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1287 2824 3005 3168 1287
Size: 1537 181 163 2993
Fragments arranged by size:
2993 1537 181 163
BtsI GCAGTG_nn'
Cuts at: 1754 2117 1754
Size: 363 4511
DraI TTT'AAA
Cuts at: 1873 1873
Size: 4874
DraIII CAC_nnn'GTG
Cuts at: 318 869 318
Size: 551 4323
DrdI GACnn_nn'nnGTC
Cuts at: 2580 3438 2580
Size: 858 4016
EaeI y'GGCC_r
Cuts at: 230 774 785 1574 2459 2633 3024 3051 4396
Size: 544 11 789 885 174 391 27 1345
Cuts at: 4396 230
Size: 708
Fragments arranged by size:
1345 885 789 708 544 391 174 27
11
EagI C'GGCC_G
Cuts at: 230 2459 230
Size: 2229 2645
EarI CTCTTCn'nnn_
Cuts at: 1677 1753 2897 3107 1677
Size: 76 1144 210 3444
Fragments arranged by size:
3444 1144 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1246 1312 3082 3392 3538 4684 1246
Size: 66 1770 310 146 1146 1436
Fragments arranged by size:
1770 1436 1146 310 146 66
EcoICRI GAG'CTC
Cuts at: 1886 1886
Size: 4874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1240 1383 2699 3131 3878 4146 427
Size: 813 143 1316 432 747 268 1155
Fragments arranged by size:
1316 1155 813 747 432 268 143
EcoO109I rG'GnC_Cy
Cuts at: 1587 2013 1587
Size: 426 4448
EcoRI G'AATT_C
Cuts at: 1878 1878
Size: 4874
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 386 1782 2893 386
Size: 1396 1111 2367
Fragments arranged by size:
2367 1396 1111
FspI TGC'GCA
Cuts at: 2655 2655
Size: 4874
HaeII r_GCGC'y
Cuts at: 328 1107 1315 1571 1766 1820 2308 2556 3578
Size: 779 208 256 195 54 488 248 1022
Cuts at: 3578 4557 328
Size: 979 645
Fragments arranged by size:
1022 979 779 645 488 256 248 208
195 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1481 1513 2716 2746 2748 2778 1481
Size: 32 1203 30 2 30 3577
Fragments arranged by size:
3577 1203 32 30 30 2
HincII GTy'rAC
Cuts at: 765 1392 1419 1913 765
Size: 627 27 494 3726
Fragments arranged by size:
3726 627 494 27
KasI G'GCGC_C
Cuts at: 1567 2304 2552 1567
Size: 737 248 3889
Fragments arranged by size:
3889 737 248
KpnI G_GTAC'C
Cuts at: 1894 1894
Size: 4874
MluI A'CGCG_T
Cuts at: 4307 4307
Size: 4874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 311 3545 3729 311
Size: 3234 184 1456
Fragments arranged by size:
3234 1456 184
MscI TGG'CCA
Cuts at: 1576 2635 1576
Size: 1059 3815
MslI CAynn'nnrTG
Cuts at: 805 1184 1378 1546 2863 2990 805
Size: 379 194 168 1317 127 2689
Fragments arranged by size:
2689 1317 379 194 168 127
MspA1I CmG'CkG
Cuts at: 165 173 1985 2299 2659 3672 3917 165
Size: 8 1812 314 360 1013 245 1122
Fragments arranged by size:
1812 1122 1013 360 314 245 8
NaeI GCC'GGC
Cuts at: 3055 3055
Size: 4874
NarI GG'CG_CC
Cuts at: 1568 2305 2553 1568
Size: 737 248 3889
Fragments arranged by size:
3889 737 248
NcoI C'CATG_G
Cuts at: 1571 1661 1571
Size: 90 4784
NgoMIV G'CCGG_C
Cuts at: 3053 3053
Size: 4874
NheI G'CTAG_C
Cuts at: 1410 1410
Size: 4874
NspI r_CATG'y
Cuts at: 804 1927 2427 3334 804
Size: 1123 500 907 2344
Fragments arranged by size:
2344 1123 907 500
PasI CC'CwG_GG
Cuts at: 1353 1353
Size: 4874
PciI A'CATG_T
Cuts at: 800 3330 800
Size: 2530 2344
PflMI CCAn_nnn'nTGG
Cuts at: 783 783
Size: 4874
PmeI GTTT'AAAC
Cuts at: 1873 1873
Size: 4874
PmlI CAC'GTG
Cuts at: 780 780
Size: 4874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 4013
Size: 32 4842
PpuMI rG'GwC_Cy
Cuts at: 1587 1587
Size: 4874
PshAI GACnn'nnGTC
Cuts at: 760 760
Size: 4874
PspOMI G'GGCC_C
Cuts at: 836 836
Size: 4874
PstI C_TGCA'G
Cuts at: 1921 2606 1921
Size: 685 4189
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4874
PvuII CAG'CTG
Cuts at: 2299 2659 2299
Size: 360 4514
RsrII CG'GwC_CG
Cuts at: 3069 3069
Size: 4874
SacI G_AGCT'C
Cuts at: 1888 1888
Size: 4874
SalI G'TCGA_C
Cuts at: 1911 1911
Size: 4874
SanDI GG'GwC_CC
Cuts at: 1587 1587
Size: 4874
SapI GCTCTTCn'nnn_
Cuts at: 1753 2897 3107 1753
Size: 1144 210 3520
Fragments arranged by size:
3520 1144 210
SbfI CC_TGCA'GG
Cuts at: 1921 1921
Size: 4874
ScaI AGT'ACT
Cuts at: 1509 4391 1509
Size: 2882 1992
SexAI A'CCwGG_T
Cuts at: 1235 1235
Size: 4874
SfcI C'TryA_G
Cuts at: 33 1458 1695 1917 2602 3595 3786 33
Size: 1425 237 222 685 993 191 1121
Fragments arranged by size:
1425 1121 993 685 237 222 191
SfoI GGC'GCC
Cuts at: 1569 2306 2554 1569
Size: 737 248 3889
Fragments arranged by size:
3889 737 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4874
SmaI CCC'GGG
Cuts at: 1896 1896
Size: 4874
SmlI C'TyrA_G
Cuts at: 682 722 2029 2944 3436 3698 682
Size: 40 1307 915 492 262 1858
Fragments arranged by size:
1858 1307 915 492 262 40
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4874
SphI G_CATG'C
Cuts at: 1927 2427 1927
Size: 500 4374
SspI AAT'ATT
Cuts at: 129 129
Size: 4874
StyI C'CwwG_G
Cuts at: 1205 1571 1661 2008 2362 1205
Size: 366 90 347 354 3717
Fragments arranged by size:
3717 366 354 347 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1665 2457 2590 3049 3258 1665
Size: 792 133 459 209 3281
Fragments arranged by size:
3281 792 459 209 133
TatI w'GTAC_w
Cuts at: 452 1316 1507 4389 452
Size: 864 191 2882 937
Fragments arranged by size:
2882 937 864 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 802 834 4391 4423 802
Size: 32 3557 32 1253
Fragments arranged by size:
3557 1253 32 32
Tth111I GACn'n_nGTC
Cuts at: 1450 2671 1450
Size: 1221 3653
XbaI T'CTAG_A
Cuts at: 1905 1905
Size: 4874
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1659 2369 1659
Size: 710 4164
XhoI C'TCGA_G
Cuts at: 722 722
Size: 4874
XmaI C'CCGG_G
Cuts at: 1894 1894
Size: 4874
ZraI GAC'GTC
Cuts at: 1433 1433
Size: 4874
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI Bpu10I BsaXI BsiWI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PfoI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI StuI SwaI XmnI