Restriction map for
pF1KB7818
(Circular) (Six-base) MAPSORT of: pF1KB7818.seq Check: 7520 from: 1 to: 4907
pF1KB7818 4907 bp
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 431 837 431
Size: 406 4501
AatII G_ACGT'C
Cuts at: 326 326
Size: 4907
AccI GT'mk_AC
Cuts at: 1226 1945 1226
Size: 719 4188
Acc65I G'GTAC_C
Cuts at: 1923 1923
Size: 4907
AclI AA'CG_TT
Cuts at: 4747 4747
Size: 4907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 2732 3164 3911 4179 270
Size: 2462 432 747 268 998
Fragments arranged by size:
2462 998 747 432 268
AflIII A'CryG_T
Cuts at: 824 998 2259 3363 4340 824
Size: 174 1261 1104 977 1391
Fragments arranged by size:
1391 1261 1104 977 174
AgeI A'CCGG_T
Cuts at: 3295 3295
Size: 4907
AleI CACnn'nnGTG
Cuts at: 664 847 664
Size: 183 4724
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1004 1004
Size: 4907
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1433 1465 1433
Size: 32 4875
AlwNI CAG_nnn'CTG
Cuts at: 2212 3779 2212
Size: 1567 3340
ApaI G_GGCC'C
Cuts at: 698 1221 698
Size: 523 4384
ApaLI G'TGCA_C
Cuts at: 3677 3677
Size: 4907
ApoI r'AATT_y
Cuts at: 1570 1911 4260 4516 1570
Size: 341 2349 256 1961
Fragments arranged by size:
2349 1961 341 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4907
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
AvaI C'yCGr_G
Cuts at: 504 543 804 1927 504
Size: 39 261 1123 3484
Fragments arranged by size:
3484 1123 261 39
BamHI G'GATC_C
Cuts at: 1932 1932
Size: 4907
BanI G'GyrC_C
Cuts at: 658 849 1851 1923 2337 2585 2620 658
Size: 191 1002 72 414 248 35 2945
Fragments arranged by size:
2945 1002 414 248 191 72 35
BanII G_rGCy'C
Cuts at: 698 725 874 1162 1172 1221 1661 1921 2951
Size: 27 149 288 10 49 440 260 1030
Cuts at: 2951 698
Size: 2654
Fragments arranged by size:
2654 1030 440 288 260 149 49 27
10
BbeI G_GCGC'C
Cuts at: 2341 2589 2341
Size: 248 4659
BbsI GAAGACnn'nnnn_
Cuts at: 392 1850 392
Size: 1458 3449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1516 1550 1591 1625 90
Size: 34 1392 34 41 34 3372
Fragments arranged by size:
3372 1392 41 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2805 3566 4907 2805
Size: 761 1341 2805
Fragments arranged by size:
2805 1341 761
BclI T'GATC_A
Cuts at: 1199 2427 1199
Size: 1228 3679
BglII A'GATC_T
Cuts at: 2422 4459 2422
Size: 2037 2870
BlpI GC'TnA_GC
Cuts at: 2019 2019
Size: 4907
Bme1580I G_kGCm'C
Cuts at: 698 770 854 1209 1221 2532 2625 3681 698
Size: 72 84 355 12 1311 93 1056 1924
Fragments arranged by size:
1924 1311 1056 355 93 84 72 12
BmgBI CAC'GTC
Cuts at: 705 705
Size: 4907
BmrI ACTGGGnnnn_n'
Cuts at: 426 1018 1471 2119 2200 2535 4679 426
Size: 592 453 648 81 335 2144 654
Fragments arranged by size:
2144 654 648 592 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3274 3274
Size: 4907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 200
Size: 4907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 1645 2083 2962 3454 3752 128
Size: 1517 438 879 492 298 1283
Fragments arranged by size:
1517 1283 879 492 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 4611 4611
Size: 4907
BsaAI yAC'GTr
Cuts at: 576 741 999 1722 2260 576
Size: 165 258 723 538 3223
Fragments arranged by size:
3223 723 538 258 165
BsaBI GATnn'nnATC
Cuts at: 6 2421 4458 6
Size: 2415 2037 455
Fragments arranged by size:
2415 2037 455
BsaHI Gr'CG_yC
Cuts at: 323 493 2338 2586 3288 4784 323
Size: 170 1845 248 702 1496 446
Fragments arranged by size:
1845 1496 702 446 248 170
BsaWI w'CCGG_w
Cuts at: 358 2096 2617 3295 3569 3716 4127 358
Size: 1738 521 678 274 147 411 1138
Fragments arranged by size:
1738 1138 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 463 493 1122 1152 463
Size: 30 629 30 4218
Fragments arranged by size:
4218 629 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 471 1566 454
Size: 17 1095 3795
Fragments arranged by size:
3795 1095 17
BseYI C'CCAG_C
Cuts at: 280 1307 1364 2332 3090 3667 4163 280
Size: 1027 57 968 758 577 496 1024
Fragments arranged by size:
1027 1024 968 758 577 496 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 538 685 538
Size: 147 4760
BsiEI CG_ry'CG
Cuts at: 104 2449 2495 3703 104
Size: 2345 46 1208 1308
Fragments arranged by size:
2345 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1921 2699 2889 3681 1921
Size: 778 190 792 3147
Fragments arranged by size:
3147 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 833 833
Size: 4907
Bsp1286I G_dGCh'C
Cuts at: 698 725 770 854 874 1162 1172 1209 1221
Size: 27 45 84 20 288 10 37 12
Cuts at: 1221 1661 1921 2532 2625 2699 2889 2951 3681
Size: 440 260 611 93 74 190 62 730
Cuts at: 3681 698
Size: 1924
Fragments arranged by size:
1924 730 611 440 288 260 190 93
84 74 62 45 37 27 20 12
10
BspEI T'CCGG_A
Cuts at: 358 4127 358
Size: 3769 1138
BspHI T'CATG_A
Cuts at: 4083 4083
Size: 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 431 837 1403 1424 1957 2171 2473 2854 431
Size: 406 566 21 533 214 302 381 2484
Fragments arranged by size:
2484 566 533 406 381 302 214 21
BsrBI CCG'CTC
Cuts at: 50 3199 3253 4737 4902 50
Size: 3149 54 1484 165 55
Fragments arranged by size:
3149 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1509 1534 1622 2819 1509
Size: 25 88 1197 3597
Fragments arranged by size:
3597 1197 88 25
BsrFI r'CCGG_y
Cuts at: 727 2905 3086 3295 727
Size: 2178 181 209 2339
Fragments arranged by size:
2339 2178 209 181
BssSI C'ACGA_G
Cuts at: 3178 3536 3178
Size: 358 4549
BstAPI GCAn_nnn'nTGC
Cuts at: 1524 2284 1524
Size: 760 4147
BstBI TT'CG_AA
Cuts at: 3268 3268
Size: 4907
BstXI CCAn_nnnn'nTGG
Cuts at: 1479 1677 1773 1479
Size: 198 96 4613
Fragments arranged by size:
4613 198 96
BstYI r'GATC_y
Cuts at: 1 547 1376 1932 2399 2422 2757 3003 4015
Size: 546 829 556 467 23 335 246 1012
Cuts at: 4015 4101 4113 4459 1
Size: 86 12 346 449
Fragments arranged by size:
1012 829 556 546 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 794 1346 794
Size: 552 4355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2857 3038 3201 2857
Size: 181 163 4563
Fragments arranged by size:
4563 181 163
BtsI GCAGTG_nn'
Cuts at: 260 2150 260
Size: 1890 3017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 320 355 320
Size: 35 4872
DraI TTT'AAA
Cuts at: 1121 1906 1121
Size: 785 4122
DraIII CAC_nnn'GTG
Cuts at: 741 744 741
Size: 3 4904
DrdI GACnn_nn'nnGTC
Cuts at: 2613 3471 2613
Size: 858 4049
EaeI y'GGCC_r
Cuts at: 578 819 2492 2666 3057 3084 4429 578
Size: 241 1673 174 391 27 1345 1056
Fragments arranged by size:
1673 1345 1056 391 241 174 27
EagI C'GGCC_G
Cuts at: 2492 2492
Size: 4907
EarI CTCTTCn'nnn_
Cuts at: 189 637 1150 2930 3140 189
Size: 448 513 1780 210 1956
Fragments arranged by size:
1956 1780 513 448 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3115 3425 3571 4717 3115
Size: 310 146 1146 3305
Fragments arranged by size:
3305 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1919 1919
Size: 4907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 270 2732 3164 3911 4179 200
Size: 70 2462 432 747 268 928
Fragments arranged by size:
2462 928 747 432 268 70
EcoO109I rG'GnC_Cy
Cuts at: 134 695 1217 2046 134
Size: 561 522 829 2995
Fragments arranged by size:
2995 829 561 522
EcoRI G'AATT_C
Cuts at: 1570 1911 1570
Size: 341 4566
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1435 2926 1435
Size: 1491 3416
FspI TGC'GCA
Cuts at: 940 2688 940
Size: 1748 3159
FspAI rTGC'GCAy
Cuts at: 940 940
Size: 4907
HaeII r_GCGC'y
Cuts at: 2341 2589 3611 4590 2341
Size: 248 1022 979 2658
Fragments arranged by size:
2658 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 532 564 1643 1675 2749 2779 2781 2811 532
Size: 32 1079 32 1074 30 2 30 2628
Fragments arranged by size:
2628 1079 1074 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1227 1946 1227
Size: 719 4188
KasI G'GCGC_C
Cuts at: 2337 2585 2337
Size: 248 4659
KpnI G_GTAC'C
Cuts at: 1927 1927
Size: 4907
MluI A'CGCG_T
Cuts at: 824 4340 824
Size: 3516 1391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 905 1256 1496 1604 3578 3762 905
Size: 351 240 108 1974 184 2050
Fragments arranged by size:
2050 1974 351 240 184 108
MscI TGG'CCA
Cuts at: 580 2668 580
Size: 2088 2819
MslI CAynn'nnrTG
Cuts at: 394 664 847 2896 3023 394
Size: 270 183 2049 127 2278
Fragments arranged by size:
2278 2049 270 183 127
MspA1I CmG'CkG
Cuts at: 834 2018 2332 2692 3705 3950 834
Size: 1184 314 360 1013 245 1791
Fragments arranged by size:
1791 1184 1013 360 314 245
NaeI GCC'GGC
Cuts at: 729 3088 729
Size: 2359 2548
NarI GG'CG_CC
Cuts at: 2338 2586 2338
Size: 248 4659
NcoI C'CATG_G
Cuts at: 794 1346 794
Size: 552 4355
NgoMIV G'CCGG_C
Cuts at: 727 3086 727
Size: 2359 2548
NspI r_CATG'y
Cuts at: 1960 2460 3367 1960
Size: 500 907 3500
Fragments arranged by size:
3500 907 500
PciI A'CATG_T
Cuts at: 3363 3363
Size: 4907
PflMI CCAn_nnn'nTGG
Cuts at: 741 744 741
Size: 3 4904
PfoI T'CCnGG_A
Cuts at: 1883 1883
Size: 4907
PmeI GTTT'AAAC
Cuts at: 1906 1906
Size: 4907
PmlI CAC'GTG
Cuts at: 741 1722 741
Size: 981 3926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 4046
Size: 32 4875
PpuMI rG'GwC_Cy
Cuts at: 134 134
Size: 4907
PshAI GACnn'nnGTC
Cuts at: 366 1034 366
Size: 668 4239
PspOMI G'GGCC_C
Cuts at: 694 1217 694
Size: 523 4384
PstI C_TGCA'G
Cuts at: 815 1954 2639 815
Size: 1139 685 3083
Fragments arranged by size:
3083 1139 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4907
PvuII CAG'CTG
Cuts at: 834 2332 2692 834
Size: 1498 360 3049
Fragments arranged by size:
3049 1498 360
RsrII CG'GwC_CG
Cuts at: 3102 3102
Size: 4907
SacI G_AGCT'C
Cuts at: 1921 1921
Size: 4907
SalI G'TCGA_C
Cuts at: 1225 1944 1225
Size: 719 4188
SapI GCTCTTCn'nnn_
Cuts at: 1150 2930 3140 1150
Size: 1780 210 2917
Fragments arranged by size:
2917 1780 210
SbfI CC_TGCA'GG
Cuts at: 1954 1954
Size: 4907
ScaI AGT'ACT
Cuts at: 1057 4424 1057
Size: 3367 1540
SfcI C'TryA_G
Cuts at: 33 171 306 811 1816 1950 2635 3628 3819
Size: 138 135 505 1005 134 685 993 191
Cuts at: 3819 33
Size: 1121
Fragments arranged by size:
1121 1005 993 685 505 191 138 135
134
SfoI GGC'GCC
Cuts at: 2339 2587 2339
Size: 248 4659
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
SmaI CCC'GGG
Cuts at: 506 545 806 1929 506
Size: 39 261 1123 3484
Fragments arranged by size:
3484 1123 261 39
SmlI C'TyrA_G
Cuts at: 143 1660 2062 2977 3469 3731 143
Size: 1517 402 915 492 262 1319
Fragments arranged by size:
1517 1319 915 492 402 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4907
SphI G_CATG'C
Cuts at: 1960 2460 1960
Size: 500 4407
StyI C'CwwG_G
Cuts at: 794 1346 2041 2395 794
Size: 552 695 354 3306
Fragments arranged by size:
3306 695 552 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1321 2490 2623 3082 3291 1321
Size: 1169 133 459 209 2937
Fragments arranged by size:
2937 1169 459 209 133
TatI w'GTAC_w
Cuts at: 1055 4422 1055
Size: 3367 1540
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 4875
Tth111I GACn'n_nGTC
Cuts at: 2704 2704
Size: 4907
XbaI T'CTAG_A
Cuts at: 1938 1938
Size: 4907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2402 2402
Size: 4907
XmaI C'CCGG_G
Cuts at: 504 543 804 1927 504
Size: 39 261 1123 3484
Fragments arranged by size:
3484 1123 261 39
XmnI GAAnn'nnTTC
Cuts at: 389 1103 389
Size: 714 4193
ZraI GAC'GTC
Cuts at: 324 324
Size: 4907
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BbvCI BfrBI
BglI BmtI Bpu10I BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI EcoNI EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI