Restriction map for
pF1KB7819
(Circular) (Six-base) MAPSORT of: pF1KB7819.seq Check: 6883 from: 1 to: 4718
pF1KB7819 4718 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1756 1756
Size: 4718
Acc65I G'GTAC_C
Cuts at: 701 1734 701
Size: 1033 3685
AclI AA'CG_TT
Cuts at: 1712 4558 1712
Size: 2846 1872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1703 2543 2975 3722 3990 1703
Size: 840 432 747 268 2431
Fragments arranged by size:
2431 840 747 432 268
AflIII A'CryG_T
Cuts at: 2070 3174 4151 2070
Size: 1104 977 2637
Fragments arranged by size:
2637 1104 977
AgeI A'CCGG_T
Cuts at: 3106 3106
Size: 4718
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 736
Size: 4718
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1078 1110 1078
Size: 32 4686
AlwNI CAG_nnn'CTG
Cuts at: 2023 3590 2023
Size: 1567 3151
ApaI G_GGCC'C
Cuts at: 1275 1352 1438 1659 1275
Size: 77 86 221 4334
Fragments arranged by size:
4334 221 86 77
ApaLI G'TGCA_C
Cuts at: 3488 3488
Size: 4718
ApoI r'AATT_y
Cuts at: 1722 4071 4327 1722
Size: 2349 256 2113
Fragments arranged by size:
2349 2113 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4718
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
AvaI C'yCGr_G
Cuts at: 282 462 1277 1738 282
Size: 180 815 461 3262
Fragments arranged by size:
3262 815 461 180
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1402 1435 1402
Size: 33 4685
BamHI G'GATC_C
Cuts at: 1743 1743
Size: 4718
BanI G'GyrC_C
Cuts at: 148 183 190 289 297 701 1386 1410 1734
Size: 35 7 99 8 404 685 24 324
Cuts at: 1734 2148 2396 2431 148
Size: 414 248 35 2435
Fragments arranged by size:
2435 685 414 404 324 248 99 35
35 24 8 7
BanII G_rGCy'C
Cuts at: 458 644 1275 1352 1438 1659 1732 2762 458
Size: 186 631 77 86 221 73 1030 2414
Fragments arranged by size:
2414 1030 631 221 186 86 77 73
BbeI G_GCGC'C
Cuts at: 187 194 293 2152 2400 187
Size: 7 99 1859 248 2505
Fragments arranged by size:
2505 1859 248 99 7
BciVI GTATCCnnnnn_n'
Cuts at: 1205 2616 3377 4718 1205
Size: 1411 761 1341 1205
Fragments arranged by size:
1411 1341 1205 761
BclI T'GATC_A
Cuts at: 578 2238 578
Size: 1660 3058
BglI GCCn_nnn'nGGC
Cuts at: 1490 1490
Size: 4718
BglII A'GATC_T
Cuts at: 2233 4270 2233
Size: 2037 2681
BlpI GC'TnA_GC
Cuts at: 1830 1830
Size: 4718
Bme1580I G_kGCm'C
Cuts at: 151 302 1275 1352 1438 1659 2343 2436 3492
Size: 151 973 77 86 221 684 93 1056
Cuts at: 3492 151
Size: 1377
Fragments arranged by size:
1377 1056 973 684 221 151 93 86
77
BmgBI CAC'GTC
Cuts at: 672 672
Size: 4718
BmrI ACTGGGnnnn_n'
Cuts at: 993 1410 1591 1930 2011 2346 4490 993
Size: 417 181 339 81 335 2144 1221
Fragments arranged by size:
2144 1221 417 339 335 181 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 4718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 658 871 1412 1508 520
Size: 138 213 541 96 3730
Fragments arranged by size:
3730 541 213 138 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1894 2773 3265 3563 1894
Size: 879 492 298 3049
Fragments arranged by size:
3049 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 278 4422 278
Size: 4144 574
BsaAI yAC'GTr
Cuts at: 2071 2071
Size: 4718
BsaBI GATnn'nnATC
Cuts at: 6 580 2232 4269 6
Size: 574 1652 2037 455
Fragments arranged by size:
2037 1652 574 455
BsaHI Gr'CG_yC
Cuts at: 184 191 290 401 856 2149 2397 3099 4595
Size: 7 99 111 455 1293 248 702 1496
Cuts at: 4595 184
Size: 307
Fragments arranged by size:
1496 1293 702 455 307 248 111 99
7
BsaWI w'CCGG_w
Cuts at: 1907 2428 3106 3380 3527 3938 1907
Size: 521 678 274 147 411 2687
Fragments arranged by size:
2687 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 763 793 1627 1657 763
Size: 30 834 30 3824
Fragments arranged by size:
3824 834 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1166 1617 1632 1166
Size: 451 15 4252
Fragments arranged by size:
4252 451 15
BseYI C'CCAG_C
Cuts at: 1204 1266 1651 2143 2901 3478 3974 1204
Size: 62 385 492 758 577 496 1948
Fragments arranged by size:
1948 758 577 496 492 385 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 742
Size: 4718
BsiEI CG_ry'CG
Cuts at: 104 359 1561 2260 2306 3514 104
Size: 255 1202 699 46 1208 1308
Fragments arranged by size:
1308 1208 1202 699 255 46
BsiHKAI G_wGCw'C
Cuts at: 887 1732 2510 2700 3492 887
Size: 845 778 190 792 2113
Fragments arranged by size:
2113 845 792 778 190
BsmI GAATG_Cn'
Cuts at: 1032 1327 1032
Size: 295 4423
Bsp1286I G_dGCh'C
Cuts at: 151 302 458 644 887 1275 1352 1438 1659
Size: 151 156 186 243 388 77 86 221
Cuts at: 1659 1732 2343 2436 2510 2700 2762 3492 151
Size: 73 611 93 74 190 62 730 1377
Fragments arranged by size:
1377 730 611 388 243 221 190 186
156 151 93 86 77 74 73 62
BspEI T'CCGG_A
Cuts at: 3938 3938
Size: 4718
BspHI T'CATG_A
Cuts at: 3894 3894
Size: 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1768 1982 2284 2665 1768
Size: 214 302 381 3821
Fragments arranged by size:
3821 381 302 214
BsrBI CCG'CTC
Cuts at: 50 710 3010 3064 4548 4713 50
Size: 660 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 660 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1004 2630 1004
Size: 1626 3092
BsrFI r'CCGG_y
Cuts at: 358 2716 2897 3106 358
Size: 2358 181 209 1970
Fragments arranged by size:
2358 1970 209 181
BsrGI T'GTAC_A
Cuts at: 1100 1100
Size: 4718
BssHII G'CGCG_C
Cuts at: 230 447 230
Size: 217 4501
BssSI C'ACGA_G
Cuts at: 2989 3347 2989
Size: 358 4360
BstAPI GCAn_nnn'nTGC
Cuts at: 804 2095 804
Size: 1291 3427
BstBI TT'CG_AA
Cuts at: 3079 3079
Size: 4718
BstYI r'GATC_y
Cuts at: 1 1743 2210 2233 2568 2814 3826 3912 3924
Size: 1742 467 23 335 246 1012 86 12
Cuts at: 3924 4270 1
Size: 346 449
Fragments arranged by size:
1742 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 395 491 1472 107
Size: 288 96 981 3353
Fragments arranged by size:
3353 981 288 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2668 2849 3012 2668
Size: 181 163 4374
Fragments arranged by size:
4374 181 163
BtsI GCAGTG_nn'
Cuts at: 730 1961 730
Size: 1231 3487
DraI TTT'AAA
Cuts at: 1717 1717
Size: 4718
DrdI GACnn_nn'nnGTC
Cuts at: 1145 2424 3282 1145
Size: 1279 858 2581
Fragments arranged by size:
2581 1279 858
EaeI y'GGCC_r
Cuts at: 356 629 1230 1439 1558 2303 2477 2868 2895
Size: 273 601 209 119 745 174 391 27
Cuts at: 2895 4240 356
Size: 1345 834
Fragments arranged by size:
1345 834 745 601 391 273 209 174
119 27
EagI C'GGCC_G
Cuts at: 356 1558 2303 356
Size: 1202 745 2771
Fragments arranged by size:
2771 1202 745
EarI CTCTTCn'nnn_
Cuts at: 2741 2951 2741
Size: 210 4508
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 615 1441 2926 3236 3382 4528 615
Size: 826 1485 310 146 1146 805
Fragments arranged by size:
1485 1146 826 805 310 146
EcoICRI GAG'CTC
Cuts at: 1730 1730
Size: 4718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 658 871 1412 1508 1703 2543 2975 3722
Size: 138 213 541 96 195 840 432 747
Cuts at: 3722 3990 520
Size: 268 1248
Fragments arranged by size:
1248 840 747 541 432 268 213 195
138 96
EcoO109I rG'GnC_Cy
Cuts at: 1271 1272 1380 1522 1655 1656 1857 1271
Size: 1 108 142 133 1 201 4132
Fragments arranged by size:
4132 201 142 133 108 1 1
EcoRI G'AATT_C
Cuts at: 1722 1722
Size: 4718
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4718
FspI TGC'GCA
Cuts at: 2499 2499
Size: 4718
HaeII r_GCGC'y
Cuts at: 187 194 293 2152 2400 3422 4401 187
Size: 7 99 1859 248 1022 979 504
Fragments arranged by size:
1859 1022 979 504 248 99 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1156 1188 1627 1659 2560 2590 2592 2622 1156
Size: 32 439 32 901 30 2 30 3252
Fragments arranged by size:
3252 901 439 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1113 1757 1113
Size: 644 4074
KasI G'GCGC_C
Cuts at: 183 190 289 2148 2396 183
Size: 7 99 1859 248 2505
Fragments arranged by size:
2505 1859 248 99 7
KpnI G_GTAC'C
Cuts at: 705 1738 705
Size: 1033 3685
MluI A'CGCG_T
Cuts at: 4151 4151
Size: 4718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 884 992 3389 3573 884
Size: 108 2397 184 2029
Fragments arranged by size:
2397 2029 184 108
MscI TGG'CCA
Cuts at: 1232 2479 1232
Size: 1247 3471
MslI CAynn'nnrTG
Cuts at: 1009 1289 2707 2834 1009
Size: 280 1418 127 2893
Fragments arranged by size:
2893 1418 280 127
MspA1I CmG'CkG
Cuts at: 397 493 1044 1445 1829 2143 2503 3516 3761
Size: 96 551 401 384 314 360 1013 245
Cuts at: 3761 397
Size: 1354
Fragments arranged by size:
1354 1013 551 401 384 360 314 245
96
NaeI GCC'GGC
Cuts at: 360 2899 360
Size: 2539 2179
NarI GG'CG_CC
Cuts at: 184 191 290 2149 2397 184
Size: 7 99 1859 248 2505
Fragments arranged by size:
2505 1859 248 99 7
NcoI C'CATG_G
Cuts at: 107 1472 107
Size: 1365 3353
NgoMIV G'CCGG_C
Cuts at: 358 2897 358
Size: 2539 2179
NspI r_CATG'y
Cuts at: 141 750 1771 2271 3178 141
Size: 609 1021 500 907 1681
Fragments arranged by size:
1681 1021 907 609 500
PasI CC'CwG_GG
Cuts at: 1345 1345
Size: 4718
PciI A'CATG_T
Cuts at: 3174 3174
Size: 4718
PfoI T'CCnGG_A
Cuts at: 1079 1079
Size: 4718
PmeI GTTT'AAAC
Cuts at: 1717 1717
Size: 4718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 4686
PpuMI rG'GwC_Cy
Cuts at: 1380 1522 1380
Size: 142 4576
PshAI GACnn'nnGTC
Cuts at: 248 1520 248
Size: 1272 3446
PsiI TTA'TAA
Cuts at: 1697 1697
Size: 4718
PspOMI G'GGCC_C
Cuts at: 1271 1348 1434 1655 1271
Size: 77 86 221 4334
Fragments arranged by size:
4334 221 86 77
PstI C_TGCA'G
Cuts at: 1765 2450 1765
Size: 685 4033
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4718
PvuII CAG'CTG
Cuts at: 1044 1445 2143 2503 1044
Size: 401 698 360 3259
Fragments arranged by size:
3259 698 401 360
RsrII CG'GwC_CG
Cuts at: 2913 2913
Size: 4718
SacI G_AGCT'C
Cuts at: 1732 1732
Size: 4718
SacII CC_GC'GG
Cuts at: 398 494 398
Size: 96 4622
SalI G'TCGA_C
Cuts at: 1755 1755
Size: 4718
SanDI GG'GwC_CC
Cuts at: 1522 1522
Size: 4718
SapI GCTCTTCn'nnn_
Cuts at: 2741 2951 2741
Size: 210 4508
SbfI CC_TGCA'GG
Cuts at: 1765 1765
Size: 4718
ScaI AGT'ACT
Cuts at: 4235 4235
Size: 4718
SfcI C'TryA_G
Cuts at: 33 654 1761 2446 3439 3630 33
Size: 621 1107 685 993 191 1121
Fragments arranged by size:
1121 1107 993 685 621 191
SfoI GGC'GCC
Cuts at: 185 192 291 2150 2398 185
Size: 7 99 1859 248 2505
Fragments arranged by size:
2505 1859 248 99 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4718
SmaI CCC'GGG
Cuts at: 284 464 1279 1740 284
Size: 180 815 461 3262
Fragments arranged by size:
3262 815 461 180
SmlI C'TyrA_G
Cuts at: 1873 2788 3280 3542 1873
Size: 915 492 262 3049
Fragments arranged by size:
3049 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4718
SphI G_CATG'C
Cuts at: 750 1771 2271 750
Size: 1021 500 3197
Fragments arranged by size:
3197 1021 500
StyI C'CwwG_G
Cuts at: 107 293 321 1155 1472 1529 1852 2206 107
Size: 186 28 834 317 57 323 354 2619
Fragments arranged by size:
2619 834 354 323 317 186 57 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 708 2301 2434 2893 3102 708
Size: 1593 133 459 209 2324
Fragments arranged by size:
2324 1593 459 209 133
TatI w'GTAC_w
Cuts at: 1100 4233 1100
Size: 3133 1585
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 763 795 4235 4267 763
Size: 32 3440 32 1214
Fragments arranged by size:
3440 1214 32 32
Tth111I GACn'n_nGTC
Cuts at: 673 2515 673
Size: 1842 2876
XbaI T'CTAG_A
Cuts at: 1749 1749
Size: 4718
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2213 114
Size: 2099 2619
XmaI C'CCGG_G
Cuts at: 282 462 1277 1738 282
Size: 180 815 461 3262
Fragments arranged by size:
3262 815 461 180
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI