Restriction map for
pF1KB7820
(Circular) (Six-base) MAPSORT of: pF1KB7820.seq Check: 2279 from: 1 to: 4910
pF1KB7820 4910 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1034 1034
Size: 4910
AccI GT'mk_AC
Cuts at: 320 1948 320
Size: 1628 3282
Acc65I G'GTAC_C
Cuts at: 1195 1353 1926 1195
Size: 158 573 4179
Fragments arranged by size:
4179 573 158
AclI AA'CG_TT
Cuts at: 4750 4750
Size: 4910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 769 802 1684 2735 3167 3914 4182 466
Size: 303 33 882 1051 432 747 268 1194
Fragments arranged by size:
1194 1051 882 747 432 303 268 33
AflII C'TTAA_G
Cuts at: 1747 1747
Size: 4910
AflIII A'CryG_T
Cuts at: 2262 3366 4343 2262
Size: 1104 977 2829
Fragments arranged by size:
2829 1104 977
AgeI A'CCGG_T
Cuts at: 3298 3298
Size: 4910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 701 701
Size: 4910
AlwNI CAG_nnn'CTG
Cuts at: 369 856 1500 2215 3782 369
Size: 487 644 715 1567 1497
Fragments arranged by size:
1567 1497 715 644 487
ApaLI G'TGCA_C
Cuts at: 3680 3680
Size: 4910
ApoI r'AATT_y
Cuts at: 1364 1708 1799 1914 4263 4519 1364
Size: 344 91 115 2349 256 1755
Fragments arranged by size:
2349 1755 344 256 115 91
AseI AT'TA_AT
Cuts at: 20 20
Size: 4910
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
AvaI C'yCGr_G
Cuts at: 162 523 1930 162
Size: 361 1407 3142
Fragments arranged by size:
3142 1407 361
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1187 1220 1187
Size: 33 4877
BamHI G'GATC_C
Cuts at: 494 1217 1935 494
Size: 723 718 3469
Fragments arranged by size:
3469 723 718
BanI G'GyrC_C
Cuts at: 151 1195 1353 1926 2340 2588 2623 151
Size: 1044 158 573 414 248 35 2438
Fragments arranged by size:
2438 1044 573 414 248 158 35
BanII G_rGCy'C
Cuts at: 212 1580 1924 2954 212
Size: 1368 344 1030 2168
Fragments arranged by size:
2168 1368 1030 344
BbeI G_GCGC'C
Cuts at: 155 2344 2592 155
Size: 2189 248 2473
Fragments arranged by size:
2473 2189 248
BbvCI CC'TCA_GC
Cuts at: 770 770
Size: 4910
BciVI GTATCCnnnnn_n'
Cuts at: 2808 3569 4910 2808
Size: 761 1341 2808
Fragments arranged by size:
2808 1341 761
BclI T'GATC_A
Cuts at: 233 2430 233
Size: 2197 2713
BglI GCCn_nnn'nGGC
Cuts at: 980 1153 980
Size: 173 4737
BglII A'GATC_T
Cuts at: 2425 4462 2425
Size: 2037 2873
BlpI GC'TnA_GC
Cuts at: 2022 2022
Size: 4910
Bme1580I G_kGCm'C
Cuts at: 2535 2628 3684 2535
Size: 93 1056 3761
Fragments arranged by size:
3761 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2122 2203 2538 4682 2122
Size: 81 335 2144 2350
Fragments arranged by size:
2350 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1250 3277 1250
Size: 2027 2883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 1170 1594 110
Size: 1060 424 3426
Fragments arranged by size:
3426 1060 424
Bpu10I CC'TnA_GC
Cuts at: 656 770 656
Size: 114 4796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 2086 2965 3457 3755 388
Size: 1698 879 492 298 1543
Fragments arranged by size:
1698 1543 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4614 4614
Size: 4910
BsaAI yAC'GTr
Cuts at: 2263 2263
Size: 4910
BsaBI GATnn'nnATC
Cuts at: 6 2424 4461 6
Size: 2418 2037 455
Fragments arranged by size:
2418 2037 455
BsaHI Gr'CG_yC
Cuts at: 152 158 2341 2589 3291 4787 152
Size: 6 2183 248 702 1496 275
Fragments arranged by size:
2183 1496 702 275 248 6
BsaWI w'CCGG_w
Cuts at: 491 2099 2620 3298 3572 3719 4130 491
Size: 1608 521 678 274 147 411 1271
Fragments arranged by size:
1608 1271 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1465 1495 1465
Size: 30 4880
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1823 1823
Size: 4910
BseYI C'CCAG_C
Cuts at: 507 2335 3093 3670 4166 507
Size: 1828 758 577 496 1251
Fragments arranged by size:
1828 1251 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 914
Size: 4910
BsiEI CG_ry'CG
Cuts at: 104 2452 2498 3706 104
Size: 2348 46 1208 1308
Fragments arranged by size:
2348 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 996 1580 1924 2702 2892 3684 996
Size: 584 344 778 190 792 2222
Fragments arranged by size:
2222 792 778 584 344 190
Bsp1286I G_dGCh'C
Cuts at: 212 996 1580 1924 2535 2628 2702 2892 2954
Size: 784 584 344 611 93 74 190 62
Cuts at: 2954 3684 212
Size: 730 1438
Fragments arranged by size:
1438 784 730 611 584 344 190 93
74 62
BspEI T'CCGG_A
Cuts at: 491 4130 491
Size: 3639 1271
BspHI T'CATG_A
Cuts at: 1836 4086 1836
Size: 2250 2660
BspMI ACCTGCnnnn'nnnn_
Cuts at: 477 1034 1960 2174 2476 2857 477
Size: 557 926 214 302 381 2530
Fragments arranged by size:
2530 926 557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1215 3202 3256 4740 4905 50
Size: 1165 1987 54 1484 165 55
Fragments arranged by size:
1987 1484 1165 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1121 1417 2822 1121
Size: 296 1405 3209
Fragments arranged by size:
3209 1405 296
BsrFI r'CCGG_y
Cuts at: 154 2908 3089 3298 154
Size: 2754 181 209 1766
Fragments arranged by size:
2754 1766 209 181
BsrGI T'GTAC_A
Cuts at: 434 434
Size: 4910
BssSI C'ACGA_G
Cuts at: 3181 3539 3181
Size: 358 4552
BstAPI GCAn_nnn'nTGC
Cuts at: 1105 1419 2287 1105
Size: 314 868 3728
Fragments arranged by size:
3728 868 314
BstBI TT'CG_AA
Cuts at: 563 3271 563
Size: 2708 2202
BstXI CCAn_nnnn'nTGG
Cuts at: 1041 1041
Size: 4910
BstYI r'GATC_y
Cuts at: 1 494 1044 1217 1935 2402 2425 2760 3006
Size: 493 550 173 718 467 23 335 246
Cuts at: 3006 4018 4104 4116 4462 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 718 550 493 467 449 346 335
246 173 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1777 1777
Size: 4910
BtgI C'CryG_G
Cuts at: 107 467 501 1109 1469 107
Size: 360 34 608 360 3548
Fragments arranged by size:
3548 608 360 360 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 778 790 2860 3041 3204 778
Size: 12 2070 181 163 2484
Fragments arranged by size:
2484 2070 181 163 12
BtsI GCAGTG_nn'
Cuts at: 1009 1305 2153 1009
Size: 296 848 3766
Fragments arranged by size:
3766 848 296
DraI TTT'AAA
Cuts at: 1909 1909
Size: 4910
DraIII CAC_nnn'GTG
Cuts at: 414 432 414
Size: 18 4892
DrdI GACnn_nn'nnGTC
Cuts at: 2616 3474 2616
Size: 858 4052
EaeI y'GGCC_r
Cuts at: 360 972 2495 2669 3060 3087 4432 360
Size: 612 1523 174 391 27 1345 838
Fragments arranged by size:
1523 1345 838 612 391 174 27
EagI C'GGCC_G
Cuts at: 2495 2495
Size: 4910
EarI CTCTTCn'nnn_
Cuts at: 442 595 821 1247 1287 1343 2933 3143 442
Size: 153 226 426 40 56 1590 210 2209
Fragments arranged by size:
2209 1590 426 226 210 153 56 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3118 3428 3574 4720 3118
Size: 310 146 1146 3308
Fragments arranged by size:
3308 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1578 1922 1578
Size: 344 4566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 466 769 802 1170 1594 1684 2735 3167
Size: 356 303 33 368 424 90 1051 432
Cuts at: 3167 3914 4182 110
Size: 747 268 838
Fragments arranged by size:
1051 838 747 432 424 368 356 303
268 90 33
EcoNI CCTnn'n_nnAGG
Cuts at: 1666 1666
Size: 4910
EcoO109I rG'GnC_Cy
Cuts at: 650 2049 650
Size: 1399 3511
EcoRI G'AATT_C
Cuts at: 1799 1914 1799
Size: 115 4795
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1401 2929 1401
Size: 1528 3382
FspI TGC'GCA
Cuts at: 2691 2691
Size: 4910
HaeII r_GCGC'y
Cuts at: 155 1662 1725 2344 2592 3614 4593 155
Size: 1507 63 619 248 1022 979 472
Fragments arranged by size:
1507 1022 979 619 472 248 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 903 935 1050 1053 1082 1085 1116 1148 1161
Size: 32 115 3 29 3 31 32 13
Cuts at: 1161 1193 1314 1346 1447 1479 1562 1594 2752
Size: 32 121 32 101 32 83 32 1158
Cuts at: 2752 2782 2784 2814 903
Size: 30 2 30 2999
Fragments arranged by size:
2999 1158 121 115 101 83 32 32
32 32 32 32 31 30 30 29
13 3 3 2
HincII GTy'rAC
Cuts at: 321 630 1484 1949 321
Size: 309 854 465 3282
Fragments arranged by size:
3282 854 465 309
HindIII A'AGCT_T
Cuts at: 796 796
Size: 4910
HpaI GTT'AAC
Cuts at: 630 630
Size: 4910
KasI G'GCGC_C
Cuts at: 151 2340 2588 151
Size: 2189 248 2473
Fragments arranged by size:
2473 2189 248
KpnI G_GTAC'C
Cuts at: 1199 1357 1930 1199
Size: 158 573 4179
Fragments arranged by size:
4179 573 158
MluI A'CGCG_T
Cuts at: 4343 4343
Size: 4910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 766 1021 3581 3765 766
Size: 255 2560 184 1911
Fragments arranged by size:
2560 1911 255 184
MscI TGG'CCA
Cuts at: 974 2671 974
Size: 1697 3213
MslI CAynn'nnrTG
Cuts at: 1114 1841 2899 3026 1114
Size: 727 1058 127 2998
Fragments arranged by size:
2998 1058 727 127
MspA1I CmG'CkG
Cuts at: 149 503 536 757 1401 1426 2021 2335 2695
Size: 354 33 221 644 25 595 314 360
Cuts at: 2695 3708 3953 149
Size: 1013 245 1106
Fragments arranged by size:
1106 1013 644 595 360 354 314 245
221 33 25
NaeI GCC'GGC
Cuts at: 156 3091 156
Size: 2935 1975
NarI GG'CG_CC
Cuts at: 152 2341 2589 152
Size: 2189 248 2473
Fragments arranged by size:
2473 2189 248
NcoI C'CATG_G
Cuts at: 107 1109 107
Size: 1002 3908
NgoMIV G'CCGG_C
Cuts at: 154 3089 154
Size: 2935 1975
NspI r_CATG'y
Cuts at: 327 1963 2463 3370 327
Size: 1636 500 907 1867
Fragments arranged by size:
1867 1636 907 500
PciI A'CATG_T
Cuts at: 3366 3366
Size: 4910
PflMI CCAn_nnn'nTGG
Cuts at: 985 985
Size: 4910
PfoI T'CCnGG_A
Cuts at: 1532 1532
Size: 4910
PmeI GTTT'AAAC
Cuts at: 1909 1909
Size: 4910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 4049
Size: 32 4878
PsiI TTA'TAA
Cuts at: 1207 1207
Size: 4910
PstI C_TGCA'G
Cuts at: 762 1016 1164 1957 2642 762
Size: 254 148 793 685 3030
Fragments arranged by size:
3030 793 685 254 148
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4910
PvuII CAG'CTG
Cuts at: 757 1401 2335 2695 757
Size: 644 934 360 2972
Fragments arranged by size:
2972 934 644 360
RsrII CG'GwC_CG
Cuts at: 3105 3105
Size: 4910
SacI G_AGCT'C
Cuts at: 1580 1924 1580
Size: 344 4566
SacII CC_GC'GG
Cuts at: 504 504
Size: 4910
SalI G'TCGA_C
Cuts at: 319 1947 319
Size: 1628 3282
SapI GCTCTTCn'nnn_
Cuts at: 2933 3143 2933
Size: 210 4700
SbfI CC_TGCA'GG
Cuts at: 1957 1957
Size: 4910
ScaI AGT'ACT
Cuts at: 4427 4427
Size: 4910
SexAI A'CCwGG_T
Cuts at: 410 410
Size: 4910
SfcI C'TryA_G
Cuts at: 33 758 1012 1160 1581 1953 2638 3631 3822
Size: 725 254 148 421 372 685 993 191
Cuts at: 3822 33
Size: 1121
Fragments arranged by size:
1121 993 725 685 421 372 254 191
148
SfoI GGC'GCC
Cuts at: 153 2342 2590 153
Size: 2189 248 2473
Fragments arranged by size:
2473 2189 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
SgrAI Cr'CCGG_yG
Cuts at: 154 154
Size: 4910
SmaI CCC'GGG
Cuts at: 164 525 1932 164
Size: 361 1407 3142
Fragments arranged by size:
3142 1407 361
SmlI C'TyrA_G
Cuts at: 403 1747 2065 2980 3472 3734 403
Size: 1344 318 915 492 262 1579
Fragments arranged by size:
1579 1344 915 492 318 262
SpeI A'CTAG_T
Cuts at: 67 1762 67
Size: 1695 3215
SphI G_CATG'C
Cuts at: 1963 2463 1963
Size: 500 4410
StuI AGG'CCT
Cuts at: 716 716
Size: 4910
StyI C'CwwG_G
Cuts at: 107 1109 1385 1703 2044 2398 107
Size: 1002 276 318 341 354 2619
Fragments arranged by size:
2619 1002 354 341 318 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 859 2493 2626 3085 3294 859
Size: 1634 133 459 209 2475
Fragments arranged by size:
2475 1634 459 209 133
TatI w'GTAC_w
Cuts at: 191 434 475 4425 191
Size: 243 41 3950 676
Fragments arranged by size:
3950 676 243 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 4878
Tth111I GACn'n_nGTC
Cuts at: 2707 2707
Size: 4910
XbaI T'CTAG_A
Cuts at: 1248 1941 1248
Size: 693 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 982 2405 982
Size: 1423 3487
XmaI C'CCGG_G
Cuts at: 162 523 1930 162
Size: 361 1407 3142
Fragments arranged by size:
3142 1407 361
XmnI GAAnn'nnTTC
Cuts at: 559 559
Size: 4910
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PspOMI PspXI PsrI SanDI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI