(Circular) (Six-base) MAPSORT of: pF1KB7822.seq Check: 5583 from: 1 to: 4913 pF1KB7822 4913 bp With 182 enzymes: SgfI * June 26, 2008 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1323 1323 Size: 4913 AatII G_ACGT'C Cuts at: 287 287 Size: 4913 AccI GT'mk_AC Cuts at: 1951 1951 Size: 4913 Acc65I G'GTAC_C Cuts at: 1295 1929 1295 Size: 634 4279 AclI AA'CG_TT Cuts at: 4753 4753 Size: 4913 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 508 1672 2738 3170 3917 4185 508 Size: 1164 1066 432 747 268 1236 Fragments arranged by size: 1236 1164 1066 747 432 268 AflIII A'CryG_T Cuts at: 2265 3369 4346 2265 Size: 1104 977 2832 Fragments arranged by size: 2832 1104 977 AgeI A'CCGG_T Cuts at: 3301 3301 Size: 4913 AleI CACnn'nnGTG Cuts at: 1039 1039 Size: 4913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 453 453 Size: 4913 AlwNI CAG_nnn'CTG Cuts at: 513 528 864 1158 2218 3785 513 Size: 15 336 294 1060 1567 1641 Fragments arranged by size: 1641 1567 1060 336 294 15 ApaLI G'TGCA_C Cuts at: 1453 3683 1453 Size: 2230 2683 ApoI r'AATT_y Cuts at: 1917 4266 4522 1917 Size: 2349 256 2308 Fragments arranged by size: 2349 2308 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4913 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4913 AvaI C'yCGr_G Cuts at: 517 804 1093 1211 1304 1739 1933 517 Size: 287 289 118 93 435 194 3497 Fragments arranged by size: 3497 435 289 287 194 118 93 BamHI G'GATC_C Cuts at: 1938 1938 Size: 4913 BanI G'GyrC_C Cuts at: 1041 1295 1701 1929 2343 2591 2626 1041 Size: 254 406 228 414 248 35 3328 Fragments arranged by size: 3328 414 406 254 248 228 35 BanII G_rGCy'C Cuts at: 314 955 994 1202 1273 1519 1629 1927 2957 Size: 641 39 208 71 246 110 298 1030 Cuts at: 2957 314 Size: 2270 Fragments arranged by size: 2270 1030 641 298 246 208 110 71 39 BbeI G_GCGC'C Cuts at: 2347 2595 2347 Size: 248 4665 BbvCI CC'TCA_GC Cuts at: 1340 1340 Size: 4913 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1833 1867 1833 Size: 34 4879 BciVI GTATCCnnnnn_n' Cuts at: 2811 3572 4913 2811 Size: 761 1341 2811 Fragments arranged by size: 2811 1341 761 BclI T'GATC_A Cuts at: 2433 2433 Size: 4913 BglI GCCn_nnn'nGGC Cuts at: 322 722 322 Size: 400 4513 BglII A'GATC_T Cuts at: 2428 4465 2428 Size: 2037 2876 BlpI GC'TnA_GC Cuts at: 2025 2025 Size: 4913 Bme1580I G_kGCm'C Cuts at: 1001 1457 2538 2631 3687 1001 Size: 456 1081 93 1056 2227 Fragments arranged by size: 2227 1081 1056 456 93 BmrI ACTGGGnnnn_n' Cuts at: 1100 1114 2125 2206 2541 4685 1100 Size: 14 1011 81 335 2144 1328 Fragments arranged by size: 2144 1328 1011 335 81 14 BmtI G_CTAG'C Cuts at: 614 614 Size: 4913 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3280 3280 Size: 4913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 198 241 490 754 1495 198 Size: 43 249 264 741 3616 Fragments arranged by size: 3616 741 264 249 43 Bpu10I CC'TnA_GC Cuts at: 1340 1576 1340 Size: 236 4677 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 271 1153 2089 2968 3460 3758 271 Size: 882 936 879 492 298 1426 Fragments arranged by size: 1426 936 882 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 606 4617 606 Size: 4011 902 BsaAI yAC'GTr Cuts at: 2266 2266 Size: 4913 BsaBI GATnn'nnATC Cuts at: 6 2427 4464 6 Size: 2421 2037 455 Fragments arranged by size: 2421 2037 455 BsaHI Gr'CG_yC Cuts at: 124 284 1349 2344 2592 3294 4790 124 Size: 160 1065 995 248 702 1496 247 Fragments arranged by size: 1496 1065 995 702 248 247 160 BsaWI w'CCGG_w Cuts at: 182 1361 2102 2623 3301 3575 3722 4133 182 Size: 1179 741 521 678 274 147 411 962 Fragments arranged by size: 1179 962 741 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 102 132 784 814 1453 1483 102 Size: 30 652 30 639 30 3532 Fragments arranged by size: 3532 652 639 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 322 1224 1251 1675 322 Size: 902 27 424 3560 Fragments arranged by size: 3560 902 424 27 BseYI C'CCAG_C Cuts at: 537 1000 1191 2338 3096 3673 4169 537 Size: 463 191 1147 758 577 496 1281 Fragments arranged by size: 1281 1147 758 577 496 463 191 BsiEI CG_ry'CG Cuts at: 104 445 790 2455 2501 3709 104 Size: 341 345 1665 46 1208 1308 Fragments arranged by size: 1665 1308 1208 345 341 46 BsiHKAI G_wGCw'C Cuts at: 314 1457 1669 1927 2705 2895 3687 314 Size: 1143 212 258 778 190 792 1540 Fragments arranged by size: 1540 1143 792 778 258 212 190 BsiWI C'GTAC_G Cuts at: 1083 1083 Size: 4913 BsmBI CGTCTCn'nnnn_ Cuts at: 131 131 Size: 4913 Bsp1286I G_dGCh'C Cuts at: 314 955 994 1001 1202 1273 1457 1519 1629 Size: 641 39 7 201 71 184 62 110 Cuts at: 1629 1669 1927 2538 2631 2705 2895 2957 3687 Size: 40 258 611 93 74 190 62 730 Cuts at: 3687 314 Size: 1540 Fragments arranged by size: 1540 730 641 611 258 201 190 184 110 93 74 71 62 62 40 39 7 BspEI T'CCGG_A Cuts at: 182 4133 182 Size: 3951 962 BspHI T'CATG_A Cuts at: 4089 4089 Size: 4913 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1323 1963 2177 2479 2860 1323 Size: 640 214 302 381 3376 Fragments arranged by size: 3376 640 381 302 214 BsrBI CCG'CTC Cuts at: 50 1374 3205 3259 4743 4908 50 Size: 1324 1831 54 1484 165 55 Fragments arranged by size: 1831 1484 1324 165 55 54 BsrDI GCAATG_nn' Cuts at: 504 2825 504 Size: 2321 2592 BsrFI r'CCGG_y Cuts at: 146 363 409 440 1580 2911 3092 3301 146 Size: 217 46 31 1140 1331 181 209 1758 Fragments arranged by size: 1758 1331 1140 217 209 181 46 31 BssHII G'CGCG_C Cuts at: 1610 1610 Size: 4913 BssSI C'ACGA_G Cuts at: 3184 3542 3184 Size: 358 4555 BstAPI GCAn_nnn'nTGC Cuts at: 1030 2290 1030 Size: 1260 3653 BstBI TT'CG_AA Cuts at: 1523 3274 1523 Size: 1751 3162 BstEII G'GTnAC_C Cuts at: 559 559 Size: 4913 BstXI CCAn_nnnn'nTGG Cuts at: 693 1127 693 Size: 434 4479 BstYI r'GATC_y Cuts at: 1 767 1938 2405 2428 2763 3009 4021 4107 Size: 766 1171 467 23 335 246 1012 86 Cuts at: 4107 4119 4465 1 Size: 12 346 449 Fragments arranged by size: 1171 1012 766 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1263 1263 Size: 4913 BtgI C'CryG_G Cuts at: 1012 1718 1012 Size: 706 4207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2863 3044 3207 2863 Size: 181 163 4569 Fragments arranged by size: 4569 181 163 BtsI GCAGTG_nn' Cuts at: 708 1846 2156 708 Size: 1138 310 3465 Fragments arranged by size: 3465 1138 310 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 741 776 741 Size: 35 4878 DraI TTT'AAA Cuts at: 1912 1912 Size: 4913 DrdI GACnn_nn'nnGTC Cuts at: 625 2619 3477 625 Size: 1994 858 2061 Fragments arranged by size: 2061 1994 858 EaeI y'GGCC_r Cuts at: 442 576 1786 2498 2672 3063 3090 4435 442 Size: 134 1210 712 174 391 27 1345 920 Fragments arranged by size: 1345 1210 920 712 391 174 134 27 EagI C'GGCC_G Cuts at: 442 2498 442 Size: 2056 2857 EarI CTCTTCn'nnn_ Cuts at: 484 1285 2936 3146 484 Size: 801 1651 210 2251 Fragments arranged by size: 2251 1651 801 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1258 3121 3431 3577 4723 1258 Size: 1863 310 146 1146 1448 Fragments arranged by size: 1863 1448 1146 310 146 EcoICRI GAG'CTC Cuts at: 312 1925 312 Size: 1613 3300 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 198 241 490 508 754 1495 1672 2738 3170 Size: 43 249 18 246 741 177 1066 432 Cuts at: 3170 3917 4185 198 Size: 747 268 926 Fragments arranged by size: 1066 926 747 741 432 268 249 246 177 43 18 EcoNI CCTnn'n_nnAGG Cuts at: 1654 1654 Size: 4913 EcoO109I rG'GnC_Cy Cuts at: 889 907 2052 889 Size: 18 1145 3750 Fragments arranged by size: 3750 1145 18 EcoRI G'AATT_C Cuts at: 1917 1917 Size: 4913 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 265 1401 2932 265 Size: 1136 1531 2246 Fragments arranged by size: 2246 1531 1136 FspI TGC'GCA Cuts at: 2694 2694 Size: 4913 HaeII r_GCGC'y Cuts at: 388 555 1878 2347 2595 3617 4596 388 Size: 167 1323 469 248 1022 979 705 Fragments arranged by size: 1323 1022 979 705 469 248 167 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 102 134 1243 1275 1398 1430 1453 1485 2755 Size: 32 1109 32 123 32 23 32 1270 Cuts at: 2755 2785 2787 2817 102 Size: 30 2 30 2198 Fragments arranged by size: 2198 1270 1109 123 32 32 32 32 30 30 23 2 HincII GTy'rAC Cuts at: 777 1320 1952 777 Size: 543 632 3738 Fragments arranged by size: 3738 632 543 HpaI GTT'AAC Cuts at: 1320 1320 Size: 4913 KasI G'GCGC_C Cuts at: 2343 2591 2343 Size: 248 4665 KpnI G_GTAC'C Cuts at: 1299 1933 1299 Size: 634 4279 MluI A'CGCG_T Cuts at: 4346 4346 Size: 4913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3584 3768 3584 Size: 184 4729 MscI TGG'CCA Cuts at: 2674 2674 Size: 4913 MslI CAynn'nnrTG Cuts at: 374 1039 2902 3029 374 Size: 665 1863 127 2258 Fragments arranged by size: 2258 1863 665 127 MspA1I CmG'CkG Cuts at: 115 525 864 1014 1155 1689 2024 2338 2698 Size: 410 339 150 141 534 335 314 360 Cuts at: 2698 3711 3956 115 Size: 1013 245 1072 Fragments arranged by size: 1072 1013 534 410 360 339 335 314 245 150 141 NaeI GCC'GGC Cuts at: 365 442 1582 3094 365 Size: 77 1140 1512 2184 Fragments arranged by size: 2184 1512 1140 77 NarI GG'CG_CC Cuts at: 2344 2592 2344 Size: 248 4665 NgoMIV G'CCGG_C Cuts at: 363 440 1580 3092 363 Size: 77 1140 1512 2184 Fragments arranged by size: 2184 1512 1140 77 NheI G'CTAG_C Cuts at: 610 610 Size: 4913 NspI r_CATG'y Cuts at: 712 1696 1966 2466 3373 712 Size: 984 270 500 907 2252 Fragments arranged by size: 2252 984 907 500 270 PasI CC'CwG_GG Cuts at: 583 1621 583 Size: 1038 3875 PciI A'CATG_T Cuts at: 3369 3369 Size: 4913 PflMI CCAn_nnn'nTGG Cuts at: 1158 1784 1158 Size: 626 4287 PfoI T'CCnGG_A Cuts at: 1471 1471 Size: 4913 PmeI GTTT'AAAC Cuts at: 1912 1912 Size: 4913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 4052 Size: 32 4881 PpuMI rG'GwC_Cy Cuts at: 907 907 Size: 4913 PspXI vC'TCGA_Gb Cuts at: 1093 1093 Size: 4913 PstI C_TGCA'G Cuts at: 158 860 1960 2645 158 Size: 702 1100 685 2426 Fragments arranged by size: 2426 1100 702 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4913 PvuII CAG'CTG Cuts at: 864 1689 2338 2698 864 Size: 825 649 360 3079 Fragments arranged by size: 3079 825 649 360 RsrII CG'GwC_CG Cuts at: 3108 3108 Size: 4913 SacI G_AGCT'C Cuts at: 314 1927 314 Size: 1613 3300 SacII CC_GC'GG Cuts at: 1015 1015 Size: 4913 SalI G'TCGA_C Cuts at: 1950 1950 Size: 4913 SanDI GG'GwC_CC Cuts at: 907 907 Size: 4913 SapI GCTCTTCn'nnn_ Cuts at: 484 2936 3146 484 Size: 2452 210 2251 Fragments arranged by size: 2452 2251 210 SbfI CC_TGCA'GG Cuts at: 1960 1960 Size: 4913 ScaI AGT'ACT Cuts at: 4430 4430 Size: 4913 SfcI C'TryA_G Cuts at: 33 154 856 1956 2641 3634 3825 33 Size: 121 702 1100 685 993 191 1121 Fragments arranged by size: 1121 1100 993 702 685 191 121 SfoI GGC'GCC Cuts at: 2345 2593 2345 Size: 248 4665 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4913 SmaI CCC'GGG Cuts at: 519 806 1213 1741 1935 519 Size: 287 407 528 194 3497 Fragments arranged by size: 3497 528 407 287 194 SmlI C'TyrA_G Cuts at: 250 1093 1132 1304 2068 2983 3475 3737 250 Size: 843 39 172 764 915 492 262 1426 Fragments arranged by size: 1426 915 843 764 492 262 172 39 SpeI A'CTAG_T Cuts at: 67 67 Size: 4913 SphI G_CATG'C Cuts at: 712 1966 2466 712 Size: 1254 500 3159 Fragments arranged by size: 3159 1254 500 SrfI GCCC'GGGC Cuts at: 519 519 Size: 4913 StyI C'CwwG_G Cuts at: 596 902 986 1238 1499 2047 2401 596 Size: 306 84 252 261 548 354 3108 Fragments arranged by size: 3108 548 354 306 261 252 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 483 596 609 999 2496 2629 3088 3297 483 Size: 113 13 390 1497 133 459 209 2099 Fragments arranged by size: 2099 1497 459 390 209 133 113 13 TatI w'GTAC_w Cuts at: 4428 4428 Size: 4913 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 784 816 4430 4462 784 Size: 32 3614 32 1235 Fragments arranged by size: 3614 1235 32 32 Tth111I GACn'n_nGTC Cuts at: 2710 2710 Size: 4913 XbaI T'CTAG_A Cuts at: 1944 1944 Size: 4913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1479 2408 1479 Size: 929 3984 XhoI C'TCGA_G Cuts at: 1093 1304 1093 Size: 211 4702 XmaI C'CCGG_G Cuts at: 517 804 1211 1739 1933 517 Size: 287 407 528 194 3497 Fragments arranged by size: 3497 528 407 287 194 ZraI GAC'GTC Cuts at: 285 285 Size: 4913 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI Enzymes that do not cut: AfeI AflII AhdI AloI ApaI AscI AvrII BaeI BbsI BfrBI BmgBI BsgI BsmI BsrGI BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII MfeI NcoI NdeI NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI PsrI SexAI SfiI SgrAI SnaBI SspI StuI SwaI XmnI