Restriction map for
pF1KB7823
(Circular) (Six-base) MAPSORT of: pF1KB7823.seq Check: 8701 from: 1 to: 4721
pF1KB7823 4721 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1366 1366
Size: 4721
AccI GT'mk_AC
Cuts at: 1557 1759 1557
Size: 202 4519
Acc65I G'GTAC_C
Cuts at: 1737 1737
Size: 4721
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 404 1231 2546 2978 3725 3993 188
Size: 216 827 1315 432 747 268 916
Fragments arranged by size:
1315 916 827 747 432 268 216
AflIII A'CryG_T
Cuts at: 1478 2073 3177 4154 1478
Size: 595 1104 977 2045
Fragments arranged by size:
2045 1104 977 595
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AlwNI CAG_nnn'CTG
Cuts at: 1228 2026 3593 1228
Size: 798 1567 2356
Fragments arranged by size:
2356 1567 798
ApaLI G'TGCA_C
Cuts at: 3491 3491
Size: 4721
ApoI r'AATT_y
Cuts at: 758 954 1391 1725 4074 4330 758
Size: 196 437 334 2349 256 1149
Fragments arranged by size:
2349 1149 437 334 256 196
AseI AT'TA_AT
Cuts at: 20 749 20
Size: 729 3992
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 764 1741 764
Size: 977 3744
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 250 283 250
Size: 33 4688
BamHI G'GATC_C
Cuts at: 1746 1746
Size: 4721
BanI G'GyrC_C
Cuts at: 1737 2151 2399 2434 1737
Size: 414 248 35 4024
Fragments arranged by size:
4024 414 248 35
BanII G_rGCy'C
Cuts at: 1735 2765 1735
Size: 1030 3691
BbeI G_GCGC'C
Cuts at: 2155 2403 2155
Size: 248 4473
BciVI GTATCCnnnnn_n'
Cuts at: 235 251 332 2619 3380 4721 235
Size: 16 81 2287 761 1341 235
Fragments arranged by size:
2287 1341 761 235 81 16
BclI T'GATC_A
Cuts at: 1407 2241 1407
Size: 834 3887
BfrBI ATG'CAT
Cuts at: 798 798
Size: 4721
BglI GCCn_nnn'nGGC
Cuts at: 967 967
Size: 4721
BglII A'GATC_T
Cuts at: 2236 4273 2236
Size: 2037 2684
BlpI GC'TnA_GC
Cuts at: 1833 1833
Size: 4721
Bme1580I G_kGCm'C
Cuts at: 1026 2346 2439 3495 1026
Size: 1320 93 1056 2252
Fragments arranged by size:
2252 1320 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 312 1451 1933 2014 2349 4493 312
Size: 1139 482 81 335 2144 540
Fragments arranged by size:
2144 1139 540 482 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 1437 698
Size: 739 3982
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 1897 2776 3268 3566 181
Size: 1716 879 492 298 1336
Fragments arranged by size:
1716 1336 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 216 4425 216
Size: 4209 512
BsaAI yAC'GTr
Cuts at: 1156 2074 1156
Size: 918 3803
BsaBI GATnn'nnATC
Cuts at: 6 2235 4272 6
Size: 2229 2037 455
Fragments arranged by size:
2229 2037 455
BsaHI Gr'CG_yC
Cuts at: 2152 2400 3102 4598 2152
Size: 248 702 1496 2275
Fragments arranged by size:
2275 1496 702 248
BsaWI w'CCGG_w
Cuts at: 245 1205 1910 2431 3109 3383 3530 3941 245
Size: 960 705 521 678 274 147 411 1025
Fragments arranged by size:
1025 960 705 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1410 1440 1410
Size: 30 4691
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 770 1719 204
Size: 566 949 3206
Fragments arranged by size:
3206 949 566
BseYI C'CCAG_C
Cuts at: 1114 2146 2904 3481 3977 1114
Size: 1032 758 577 496 1858
Fragments arranged by size:
1858 1032 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 116
Size: 4721
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3517 104
Size: 2159 46 1208 1308
Fragments arranged by size:
2159 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1735 2513 2703 3495 1735
Size: 778 190 792 2961
Fragments arranged by size:
2961 792 778 190
BsiWI C'GTAC_G
Cuts at: 236 236
Size: 4721
BsmI GAATG_Cn'
Cuts at: 363 489 667 798 363
Size: 126 178 131 4286
Fragments arranged by size:
4286 178 131 126
Bsp1286I G_dGCh'C
Cuts at: 1026 1735 2346 2439 2513 2703 2765 3495 1026
Size: 709 611 93 74 190 62 730 2252
Fragments arranged by size:
2252 730 709 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3941 3941
Size: 4721
BspHI T'CATG_A
Cuts at: 3897 3897
Size: 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 138 509 1366 1771 1985 2287 2668 138
Size: 371 857 405 214 302 381 2191
Fragments arranged by size:
2191 857 405 381 371 302 214
BsrBI CCG'CTC
Cuts at: 50 3013 3067 4551 4716 50
Size: 2963 54 1484 165 55
Fragments arranged by size:
2963 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 983 2633 983
Size: 1650 3071
BsrFI r'CCGG_y
Cuts at: 2719 2900 3109 2719
Size: 181 209 4331
Fragments arranged by size:
4331 209 181
BssSI C'ACGA_G
Cuts at: 1001 1684 2992 3350 1001
Size: 683 1308 358 2372
Fragments arranged by size:
2372 1308 683 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2098 2098
Size: 4721
BstBI TT'CG_AA
Cuts at: 3082 3082
Size: 4721
BstXI CCAn_nnnn'nTGG
Cuts at: 375 1359 375
Size: 984 3737
BstYI r'GATC_y
Cuts at: 1 181 305 720 1596 1746 2213 2236 2571
Size: 180 124 415 876 150 467 23 335
Cuts at: 2571 2817 3829 3915 3927 4273 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 876 467 449 415 346 335 246
180 150 124 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4721
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 1964 1964
Size: 4721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 820 855 820
Size: 35 4686
DraI TTT'AAA
Cuts at: 1720 1720
Size: 4721
DrdI GACnn_nn'nnGTC
Cuts at: 2427 3285 2427
Size: 858 3863
EaeI y'GGCC_r
Cuts at: 365 2306 2480 2871 2898 4243 365
Size: 1941 174 391 27 1345 843
Fragments arranged by size:
1941 1345 843 391 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 216 918 1064 1104 1345 1696 2744 2954 216
Size: 702 146 40 241 351 1048 210 1983
Fragments arranged by size:
1983 1048 702 351 241 210 146 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2929 3239 3385 4531 2929
Size: 310 146 1146 3119
Fragments arranged by size:
3119 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1733 1733
Size: 4721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 404 698 1231 1437 2546 2978 3725 3993
Size: 216 294 533 206 1109 432 747 268
Cuts at: 3993 188
Size: 916
Fragments arranged by size:
1109 916 747 533 432 294 268 216
206
EcoO109I rG'GnC_Cy
Cuts at: 1860 1860
Size: 4721
EcoRI G'AATT_C
Cuts at: 1725 1725
Size: 4721
EcoRV GAT'ATC
Cuts at: 1269 1269
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1093 2740 1093
Size: 1647 3074
FspI TGC'GCA
Cuts at: 2502 2502
Size: 4721
HaeII r_GCGC'y
Cuts at: 115 2155 2403 3425 4404 115
Size: 2040 248 1022 979 432
Fragments arranged by size:
2040 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1103 1110 1135 1142 1222 1254 2563 2593 2595
Size: 7 25 7 80 32 1309 30 2
Cuts at: 2595 2625 1103
Size: 30 3199
Fragments arranged by size:
3199 1309 80 32 30 30 25 7
7 2
HincII GTy'rAC
Cuts at: 1760 1760
Size: 4721
KasI G'GCGC_C
Cuts at: 2151 2399 2151
Size: 248 4473
KpnI G_GTAC'C
Cuts at: 1741 1741
Size: 4721
MfeI C'AATT_G
Cuts at: 407 407
Size: 4721
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3392 3576 3392
Size: 184 4537
MscI TGG'CCA
Cuts at: 367 2482 367
Size: 2115 2606
MslI CAynn'nnrTG
Cuts at: 373 947 1688 2710 2837 373
Size: 574 741 1022 127 2257
Fragments arranged by size:
2257 1022 741 574 127
MspA1I CmG'CkG
Cuts at: 610 1832 2146 2506 3519 3764 610
Size: 1222 314 360 1013 245 1567
Fragments arranged by size:
1567 1222 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2902 2902
Size: 4721
NarI GG'CG_CC
Cuts at: 2152 2400 2152
Size: 248 4473
NcoI C'CATG_G
Cuts at: 107 107
Size: 4721
NgoMIV G'CCGG_C
Cuts at: 2900 2900
Size: 4721
NsiI A_TGCA'T
Cuts at: 800 800
Size: 4721
NspI r_CATG'y
Cuts at: 1482 1774 2274 3181 1482
Size: 292 500 907 3022
Fragments arranged by size:
3022 907 500 292
PciI A'CATG_T
Cuts at: 1478 3177 1478
Size: 1699 3022
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PmlI CAC'GTG
Cuts at: 1156 1156
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4689
PshAI GACnn'nnGTC
Cuts at: 905 905
Size: 4721
PsiI TTA'TAA
Cuts at: 1397 1397
Size: 4721
PstI C_TGCA'G
Cuts at: 1768 2453 1768
Size: 685 4036
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 610 2146 2506 610
Size: 1536 360 2825
Fragments arranged by size:
2825 1536 360
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 1735 1735
Size: 4721
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 1104 1345 2744 2954 1104
Size: 241 1399 210 2871
Fragments arranged by size:
2871 1399 241 210
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SexAI A'CCwGG_T
Cuts at: 461 461
Size: 4721
SfcI C'TryA_G
Cuts at: 33 931 1764 2449 3442 3633 33
Size: 898 833 685 993 191 1121
Fragments arranged by size:
1121 993 898 833 685 191
SfoI GGC'GCC
Cuts at: 2153 2401 2153
Size: 248 4473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 766 1743 766
Size: 977 3744
SmlI C'TyrA_G
Cuts at: 160 1876 2791 3283 3545 160
Size: 1716 915 492 262 1336
Fragments arranged by size:
1716 1336 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4721
SphI G_CATG'C
Cuts at: 1774 2274 1774
Size: 500 4221
StyI C'CwwG_G
Cuts at: 107 736 1352 1676 1855 2209 107
Size: 629 616 324 179 354 2619
Fragments arranged by size:
2619 629 616 354 324 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2304 2437 2896 3105 2304
Size: 133 459 209 3920
Fragments arranged by size:
3920 459 209 133
TatI w'GTAC_w
Cuts at: 939 4236 939
Size: 3297 1424
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 987 1019 4238 4270 987
Size: 32 3219 32 1438
Fragments arranged by size:
3219 1438 32 32
Tth111I GACn'n_nGTC
Cuts at: 436 2518 436
Size: 2082 2639
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1464 2216 1464
Size: 752 3969
XmaI C'CCGG_G
Cuts at: 764 1741 764
Size: 977 3744
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BbsI BbvCI BcgI BmgBI BmtI Bpu10I
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PasI PflMI PfoI PpuMI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI