Restriction map for
pF1KB7826
(Circular) (Six-base) MAPSORT of: pF1KB7826.seq Check: 2820 from: 1 to: 4925
pF1KB7826 4925 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1963 1963
Size: 4925
Acc65I G'GTAC_C
Cuts at: 1941 1941
Size: 4925
AclI AA'CG_TT
Cuts at: 851 4765 851
Size: 3914 1011
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 525 1008 1428 2750 3182 3929 4197 525
Size: 483 420 1322 432 747 268 1253
Fragments arranged by size:
1322 1253 747 483 432 420 268
AflIII A'CryG_T
Cuts at: 925 2277 3381 4358 925
Size: 1352 1104 977 1492
Fragments arranged by size:
1492 1352 1104 977
AgeI A'CCGG_T
Cuts at: 3313 3313
Size: 4925
AleI CACnn'nnGTG
Cuts at: 1403 1403
Size: 4925
AlwNI CAG_nnn'CTG
Cuts at: 577 2230 3797 577
Size: 1653 1567 1705
Fragments arranged by size:
1705 1653 1567
ApaLI G'TGCA_C
Cuts at: 3695 3695
Size: 4925
ApoI r'AATT_y
Cuts at: 974 1394 1646 1730 1898 1929 4278 4534 974
Size: 420 252 84 168 31 2349 256 1365
Fragments arranged by size:
2349 1365 420 256 252 168 84 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 4925
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
AvaI C'yCGr_G
Cuts at: 1945 1945
Size: 4925
BamHI G'GATC_C
Cuts at: 1950 1950
Size: 4925
BanI G'GyrC_C
Cuts at: 1941 2355 2603 2638 1941
Size: 414 248 35 4228
Fragments arranged by size:
4228 414 248 35
BanII G_rGCy'C
Cuts at: 788 1314 1939 2969 788
Size: 526 625 1030 2744
Fragments arranged by size:
2744 1030 625 526
BbeI G_GCGC'C
Cuts at: 2359 2607 2359
Size: 248 4677
BbsI GAAGACnn'nnnn_
Cuts at: 1459 1459
Size: 4925
BciVI GTATCCnnnnn_n'
Cuts at: 263 2823 3584 4925 263
Size: 2560 761 1341 263
Fragments arranged by size:
2560 1341 761 263
BclI T'GATC_A
Cuts at: 744 2445 744
Size: 1701 3224
BglII A'GATC_T
Cuts at: 2440 4477 2440
Size: 2037 2888
BlpI GC'TnA_GC
Cuts at: 2037 2037
Size: 4925
Bme1580I G_kGCm'C
Cuts at: 2550 2643 3699 2550
Size: 93 1056 3776
Fragments arranged by size:
3776 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 393 616 1076 1160 1244 1496 1832 2137 2218
Size: 223 460 84 84 252 336 305 81
Cuts at: 2218 2553 4697 393
Size: 335 2144 621
Fragments arranged by size:
2144 621 460 336 335 305 252 223
84 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 4925
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1340 1592 241
Size: 1099 252 3574
Fragments arranged by size:
3574 1099 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 2101 2980 3472 3770 820
Size: 1281 879 492 298 1975
Fragments arranged by size:
1975 1281 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 246 4629 246
Size: 4383 542
BsaAI yAC'GTr
Cuts at: 2278 2278
Size: 4925
BsaBI GATnn'nnATC
Cuts at: 6 350 836 2439 4476 6
Size: 344 486 1603 2037 455
Fragments arranged by size:
2037 1603 486 455 344
BsaHI Gr'CG_yC
Cuts at: 449 2356 2604 3306 4802 449
Size: 1907 248 702 1496 572
Fragments arranged by size:
1907 1496 702 572 248
BsaWI w'CCGG_w
Cuts at: 2114 2635 3313 3587 3734 4145 2114
Size: 521 678 274 147 411 2894
Fragments arranged by size:
2894 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 181
Size: 4925
BseYI C'CCAG_C
Cuts at: 2350 3108 3685 4181 2350
Size: 758 577 496 3094
Fragments arranged by size:
3094 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2467 2513 3721 104
Size: 2363 46 1208 1308
Fragments arranged by size:
2363 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 475 1314 1428 1939 2717 2907 3699 475
Size: 839 114 511 778 190 792 1701
Fragments arranged by size:
1701 839 792 778 511 190 114
BsmI GAATG_Cn'
Cuts at: 1183 1183
Size: 4925
Bsp1286I G_dGCh'C
Cuts at: 475 788 1314 1428 1939 2550 2643 2717 2907
Size: 313 526 114 511 611 93 74 190
Cuts at: 2907 2969 3699 475
Size: 62 730 1701
Fragments arranged by size:
1701 730 611 526 511 313 190 114
93 74 62
BspEI T'CCGG_A
Cuts at: 4145 4145
Size: 4925
BspHI T'CATG_A
Cuts at: 4101 4101
Size: 4925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 537 1975 2189 2491 2872 537
Size: 1438 214 302 381 2590
Fragments arranged by size:
2590 1438 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3217 3271 4755 4920 50
Size: 3167 54 1484 165 55
Fragments arranged by size:
3167 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2837 2837
Size: 4925
BsrFI r'CCGG_y
Cuts at: 2923 3104 3313 2923
Size: 181 209 4535
Fragments arranged by size:
4535 209 181
BsrGI T'GTAC_A
Cuts at: 203 963 1047 1887 203
Size: 760 84 840 3241
Fragments arranged by size:
3241 840 760 84
BssSI C'ACGA_G
Cuts at: 3196 3554 3196
Size: 358 4567
BstAPI GCAn_nnn'nTGC
Cuts at: 2302 2302
Size: 4925
BstBI TT'CG_AA
Cuts at: 3286 3286
Size: 4925
BstYI r'GATC_y
Cuts at: 1 335 1950 2417 2440 2775 3021 4033 4119
Size: 334 1615 467 23 335 246 1012 86
Cuts at: 4119 4131 4477 1
Size: 12 346 449
Fragments arranged by size:
1615 1012 467 449 346 335 334 246
86 23 12
BtgI C'CryG_G
Cuts at: 304 304
Size: 4925
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2875 3056 3219 2875
Size: 181 163 4581
Fragments arranged by size:
4581 181 163
BtsI GCAGTG_nn'
Cuts at: 2168 2168
Size: 4925
DraI TTT'AAA
Cuts at: 482 678 1924 482
Size: 196 1246 3483
Fragments arranged by size:
3483 1246 196
DraIII CAC_nnn'GTG
Cuts at: 1908 1908
Size: 4925
DrdI GACnn_nn'nnGTC
Cuts at: 2631 3489 2631
Size: 858 4067
EaeI y'GGCC_r
Cuts at: 2510 2684 3075 3102 4447 2510
Size: 174 391 27 1345 2988
Fragments arranged by size:
2988 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2510 2510
Size: 4925
EarI CTCTTCn'nnn_
Cuts at: 293 373 544 2948 3158 293
Size: 80 171 2404 210 2060
Fragments arranged by size:
2404 2060 210 171 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3133 3443 3589 4735 3133
Size: 310 146 1146 3323
Fragments arranged by size:
3323 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1312 1937 1312
Size: 625 4300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 525 1008 1340 1428 1592 2750 3182 3929
Size: 284 483 332 88 164 1158 432 747
Cuts at: 3929 4197 241
Size: 268 969
Fragments arranged by size:
1158 969 747 483 432 332 284 268
164 88
EcoNI CCTnn'n_nnAGG
Cuts at: 1446 1446
Size: 4925
EcoO109I rG'GnC_Cy
Cuts at: 197 2064 197
Size: 1867 3058
EcoRI G'AATT_C
Cuts at: 974 1394 1646 1730 1898 1929 974
Size: 420 252 84 168 31 3970
Fragments arranged by size:
3970 420 252 168 84 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4925
FspI TGC'GCA
Cuts at: 2706 2706
Size: 4925
HaeII r_GCGC'y
Cuts at: 2359 2607 3629 4608 2359
Size: 248 1022 979 2676
Fragments arranged by size:
2676 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 2767 2797 2799 2829 262
Size: 32 2473 30 2 30 2358
Fragments arranged by size:
2473 2358 32 30 30 2
HincII GTy'rAC
Cuts at: 561 1964 561
Size: 1403 3522
HpaI GTT'AAC
Cuts at: 561 561
Size: 4925
KasI G'GCGC_C
Cuts at: 2355 2603 2355
Size: 248 4677
KpnI G_GTAC'C
Cuts at: 1945 1945
Size: 4925
MluI A'CGCG_T
Cuts at: 4358 4358
Size: 4925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 3596 3780 266
Size: 3330 184 1411
Fragments arranged by size:
3330 1411 184
MscI TGG'CCA
Cuts at: 2686 2686
Size: 4925
MslI CAynn'nnrTG
Cuts at: 665 1403 2914 3041 665
Size: 738 1511 127 2549
Fragments arranged by size:
2549 1511 738 127
MspA1I CmG'CkG
Cuts at: 507 1293 2036 2350 2710 3723 3968 507
Size: 786 743 314 360 1013 245 1464
Fragments arranged by size:
1464 1013 786 743 360 314 245
NaeI GCC'GGC
Cuts at: 3106 3106
Size: 4925
NarI GG'CG_CC
Cuts at: 2356 2604 2356
Size: 248 4677
NcoI C'CATG_G
Cuts at: 304 304
Size: 4925
NdeI CA'TA_TG
Cuts at: 996 1756 996
Size: 760 4165
NgoMIV G'CCGG_C
Cuts at: 3104 3104
Size: 4925
NspI r_CATG'y
Cuts at: 929 1013 1978 2478 3385 929
Size: 84 965 500 907 2469
Fragments arranged by size:
2469 965 907 500 84
PasI CC'CwG_GG
Cuts at: 120 120
Size: 4925
PciI A'CATG_T
Cuts at: 925 3381 925
Size: 2456 2469
PfoI T'CCnGG_A
Cuts at: 1716 1716
Size: 4925
PmeI GTTT'AAAC
Cuts at: 1924 1924
Size: 4925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4893
PpuMI rG'GwC_Cy
Cuts at: 197 197
Size: 4925
PsiI TTA'TAA
Cuts at: 719 719
Size: 4925
PstI C_TGCA'G
Cuts at: 512 1972 2657 512
Size: 1460 685 2780
Fragments arranged by size:
2780 1460 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4925
PvuII CAG'CTG
Cuts at: 507 1293 2350 2710 507
Size: 786 1057 360 2722
Fragments arranged by size:
2722 1057 786 360
RsrII CG'GwC_CG
Cuts at: 3120 3120
Size: 4925
SacI G_AGCT'C
Cuts at: 1314 1939 1314
Size: 625 4300
SalI G'TCGA_C
Cuts at: 1962 1962
Size: 4925
SapI GCTCTTCn'nnn_
Cuts at: 293 2948 3158 293
Size: 2655 210 2060
Fragments arranged by size:
2655 2060 210
SbfI CC_TGCA'GG
Cuts at: 1972 1972
Size: 4925
ScaI AGT'ACT
Cuts at: 4442 4442
Size: 4925
SexAI A'CCwGG_T
Cuts at: 236 236
Size: 4925
SfcI C'TryA_G
Cuts at: 33 228 508 1968 2653 3646 3837 33
Size: 195 280 1460 685 993 191 1121
Fragments arranged by size:
1460 1121 993 685 280 195 191
SfoI GGC'GCC
Cuts at: 2357 2605 2357
Size: 248 4677
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
SmaI CCC'GGG
Cuts at: 1947 1947
Size: 4925
SmlI C'TyrA_G
Cuts at: 799 2080 2995 3487 3749 799
Size: 1281 915 492 262 1975
Fragments arranged by size:
1975 1281 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4925
SphI G_CATG'C
Cuts at: 1013 1978 2478 1013
Size: 965 500 3460
Fragments arranged by size:
3460 965 500
SspI AAT'ATT
Cuts at: 617 699 617
Size: 82 4843
StuI AGG'CCT
Cuts at: 1273 1777 1861 1273
Size: 504 84 4337
Fragments arranged by size:
4337 504 84
StyI C'CwwG_G
Cuts at: 304 2059 2413 304
Size: 1755 354 2816
Fragments arranged by size:
2816 1755 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 175 2508 2641 3100 3309 175
Size: 2333 133 459 209 1791
Fragments arranged by size:
2333 1791 459 209 133
TatI w'GTAC_w
Cuts at: 203 963 1047 1507 1675 1887 4440 203
Size: 760 84 460 168 212 2553 688
Fragments arranged by size:
2553 760 688 460 212 168 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4442 4474 4442
Size: 32 4893
Tth111I GACn'n_nGTC
Cuts at: 2722 2722
Size: 4925
XbaI T'CTAG_A
Cuts at: 519 1956 519
Size: 1437 3488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 2420
Size: 4925
XmaI C'CCGG_G
Cuts at: 1945 1945
Size: 4925
XmnI GAAnn'nnTTC
Cuts at: 1189 1357 1693 1861 1189
Size: 168 336 168 4253
Fragments arranged by size:
4253 336 168 168
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI
HindIII MfeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI