Restriction map for
pF1KB7828
(Circular) (Six-base) MAPSORT of: pF1KB7828.seq Check: 4244 from: 1 to: 4928
pF1KB7828 4928 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1020 1020
Size: 4928
AccI GT'mk_AC
Cuts at: 1966 1966
Size: 4928
Acc65I G'GTAC_C
Cuts at: 1944 1944
Size: 4928
AclI AA'CG_TT
Cuts at: 1922 4768 1922
Size: 2846 2082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 852 1256 2753 3185 3932 4200 207
Size: 645 404 1497 432 747 268 935
Fragments arranged by size:
1497 935 747 645 432 404 268
AflIII A'CryG_T
Cuts at: 2280 3384 4361 2280
Size: 1104 977 2847
Fragments arranged by size:
2847 1104 977
AgeI A'CCGG_T
Cuts at: 3316 3316
Size: 4928
AleI CACnn'nnGTG
Cuts at: 1354 1354
Size: 4928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1018 1372 1018
Size: 354 4574
AlwNI CAG_nnn'CTG
Cuts at: 2233 3800 2233
Size: 1567 3361
ApaI G_GGCC'C
Cuts at: 1055 1055
Size: 4928
ApaLI G'TGCA_C
Cuts at: 3698 3698
Size: 4928
ApoI r'AATT_y
Cuts at: 352 921 958 1120 1932 4281 4537 352
Size: 569 37 162 812 2349 256 743
Fragments arranged by size:
2349 812 743 569 256 162 37
AseI AT'TA_AT
Cuts at: 20 1597 20
Size: 1577 3351
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 1948 1948
Size: 4928
BamHI G'GATC_C
Cuts at: 1953 1953
Size: 4928
BanI G'GyrC_C
Cuts at: 1944 2358 2606 2641 1944
Size: 414 248 35 4231
Fragments arranged by size:
4231 414 248 35
BanII G_rGCy'C
Cuts at: 568 986 1055 1942 2972 568
Size: 418 69 887 1030 2524
Fragments arranged by size:
2524 1030 887 418 69
BbeI G_GCGC'C
Cuts at: 2362 2610 2362
Size: 248 4680
BbsI GAAGACnn'nnnn_
Cuts at: 564 896 564
Size: 332 4596
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3587 4928 2826
Size: 761 1341 2826
Fragments arranged by size:
2826 1341 761
BclI T'GATC_A
Cuts at: 2448 2448
Size: 4928
BglII A'GATC_T
Cuts at: 192 2443 4480 192
Size: 2251 2037 640
Fragments arranged by size:
2251 2037 640
BlpI GC'TnA_GC
Cuts at: 980 2040 980
Size: 1060 3868
Bme1580I G_kGCm'C
Cuts at: 1055 1260 2553 2646 3702 1055
Size: 205 1293 93 1056 2281
Fragments arranged by size:
2281 1293 1056 205 93
BmrI ACTGGGnnnn_n'
Cuts at: 562 980 1157 2140 2221 2556 4700 562
Size: 418 177 983 81 335 2144 790
Fragments arranged by size:
2144 983 790 418 335 177 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3295 3295
Size: 4928
Bpu10I CC'TnA_GC
Cuts at: 617 617
Size: 4928
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2104 2983 3475 3773 2104
Size: 879 492 298 3259
Fragments arranged by size:
3259 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4632 4632
Size: 4928
BsaAI yAC'GTr
Cuts at: 1356 1612 1813 2281 1356
Size: 256 201 468 4003
Fragments arranged by size:
4003 468 256 201
BsaBI GATnn'nnATC
Cuts at: 6 191 1694 2442 4479 6
Size: 185 1503 748 2037 455
Fragments arranged by size:
2037 1503 748 455 185
BsaHI Gr'CG_yC
Cuts at: 2359 2607 3309 4805 2359
Size: 248 702 1496 2482
Fragments arranged by size:
2482 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2117 2638 3316 3590 3737 4148 2117
Size: 521 678 274 147 411 2897
Fragments arranged by size:
2897 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 688 688
Size: 4928
BseYI C'CCAG_C
Cuts at: 146 374 2353 3111 3688 4184 146
Size: 228 1979 758 577 496 890
Fragments arranged by size:
1979 890 758 577 496 228
BsiEI CG_ry'CG
Cuts at: 104 2470 2516 3724 104
Size: 2366 46 1208 1308
Fragments arranged by size:
2366 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1351 1942 2720 2910 3702 1351
Size: 591 778 190 792 2577
Fragments arranged by size:
2577 792 778 591 190
Bsp1286I G_dGCh'C
Cuts at: 568 986 1055 1260 1351 1942 2553 2646 2720
Size: 418 69 205 91 591 611 93 74
Cuts at: 2720 2910 2972 3702 568
Size: 190 62 730 1794
Fragments arranged by size:
1794 730 611 591 418 205 190 93
91 74 69 62
BspEI T'CCGG_A
Cuts at: 4148 4148
Size: 4928
BspHI T'CATG_A
Cuts at: 4104 4104
Size: 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1020 1978 2192 2494 2875 1020
Size: 958 214 302 381 3073
Fragments arranged by size:
3073 958 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3220 3274 4758 4923 50
Size: 3170 54 1484 165 55
Fragments arranged by size:
3170 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 575 1881 2840 575
Size: 1306 959 2663
Fragments arranged by size:
2663 1306 959
BsrFI r'CCGG_y
Cuts at: 2926 3107 3316 2926
Size: 181 209 4538
Fragments arranged by size:
4538 209 181
BssSI C'ACGA_G
Cuts at: 3199 3557 3199
Size: 358 4570
BstAPI GCAn_nnn'nTGC
Cuts at: 2305 2305
Size: 4928
BstBI TT'CG_AA
Cuts at: 3289 3289
Size: 4928
BstYI r'GATC_y
Cuts at: 1 192 400 1953 2420 2443 2778 3024 4036
Size: 191 208 1553 467 23 335 246 1012
Cuts at: 4036 4122 4134 4480 1
Size: 86 12 346 449
Fragments arranged by size:
1553 1012 467 449 346 335 246 208
191 86 23 12
BtgI C'CryG_G
Cuts at: 740 740
Size: 4928
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2878 3059 3222 2878
Size: 181 163 4584
Fragments arranged by size:
4584 181 163
BtsI GCAGTG_nn'
Cuts at: 2171 2171
Size: 4928
DraI TTT'AAA
Cuts at: 1927 1927
Size: 4928
DraIII CAC_nnn'GTG
Cuts at: 1188 1188
Size: 4928
DrdI GACnn_nn'nnGTC
Cuts at: 2634 3492 2634
Size: 858 4070
EaeI y'GGCC_r
Cuts at: 2513 2687 3078 3105 4450 2513
Size: 174 391 27 1345 2991
Fragments arranged by size:
2991 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2513 2513
Size: 4928
EarI CTCTTCn'nnn_
Cuts at: 335 1238 2951 3161 335
Size: 903 1713 210 2102
Fragments arranged by size:
2102 1713 903 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3136 3446 3592 4738 3136
Size: 310 146 1146 3326
Fragments arranged by size:
3326 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1940 1940
Size: 4928
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 852 1256 2753 3185 3932 4200 207
Size: 645 404 1497 432 747 268 935
Fragments arranged by size:
1497 935 747 645 432 404 268
EcoO109I rG'GnC_Cy
Cuts at: 2067 2067
Size: 4928
EcoRI G'AATT_C
Cuts at: 1120 1932 1120
Size: 812 4116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1606 2947 1606
Size: 1341 3587
FspI TGC'GCA
Cuts at: 2709 2709
Size: 4928
HaeII r_GCGC'y
Cuts at: 2362 2610 3632 4611 2362
Size: 248 1022 979 2679
Fragments arranged by size:
2679 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 1120 1152 2770 2800 2802 2832 667
Size: 32 421 32 1618 30 2 30 2763
Fragments arranged by size:
2763 1618 421 32 32 30 30 2
HincII GTy'rAC
Cuts at: 777 905 939 1545 1967 777
Size: 128 34 606 422 3738
Fragments arranged by size:
3738 606 422 128 34
HindIII A'AGCT_T
Cuts at: 1085 1085
Size: 4928
KasI G'GCGC_C
Cuts at: 2358 2606 2358
Size: 248 4680
KpnI G_GTAC'C
Cuts at: 1948 1948
Size: 4928
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3599 3783 3599
Size: 184 4744
MscI TGG'CCA
Cuts at: 2689 2689
Size: 4928
MslI CAynn'nnrTG
Cuts at: 1354 1358 2917 3044 1354
Size: 4 1559 127 3238
Fragments arranged by size:
3238 1559 127 4
MspA1I CmG'CkG
Cuts at: 2039 2353 2713 3726 3971 2039
Size: 314 360 1013 245 2996
Fragments arranged by size:
2996 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3109 3109
Size: 4928
NarI GG'CG_CC
Cuts at: 2359 2607 2359
Size: 248 4680
NcoI C'CATG_G
Cuts at: 740 740
Size: 4928
NgoMIV G'CCGG_C
Cuts at: 3107 3107
Size: 4928
NspI r_CATG'y
Cuts at: 468 1005 1809 1981 2481 3388 468
Size: 537 804 172 500 907 2008
Fragments arranged by size:
2008 907 804 537 500 172
PciI A'CATG_T
Cuts at: 3384 3384
Size: 4928
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PmlI CAC'GTG
Cuts at: 1356 1356
Size: 4928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 4067
Size: 32 4896
PshAI GACnn'nnGTC
Cuts at: 141 224 141
Size: 83 4845
PspOMI G'GGCC_C
Cuts at: 1051 1051
Size: 4928
PstI C_TGCA'G
Cuts at: 1859 1975 2660 1859
Size: 116 685 4127
Fragments arranged by size:
4127 685 116
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 2353 2713 2353
Size: 360 4568
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 1942 1942
Size: 4928
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SapI GCTCTTCn'nnn_
Cuts at: 1238 2951 3161 1238
Size: 1713 210 3005
Fragments arranged by size:
3005 1713 210
SbfI CC_TGCA'GG
Cuts at: 1975 1975
Size: 4928
ScaI AGT'ACT
Cuts at: 4445 4445
Size: 4928
SfcI C'TryA_G
Cuts at: 33 831 1514 1855 1971 2656 3649 3840 33
Size: 798 683 341 116 685 993 191 1121
Fragments arranged by size:
1121 993 798 685 683 341 191 116
SfoI GGC'GCC
Cuts at: 2360 2608 2360
Size: 248 4680
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SmaI CCC'GGG
Cuts at: 1950 1950
Size: 4928
SmlI C'TyrA_G
Cuts at: 2083 2998 3490 3752 2083
Size: 915 492 262 3259
Fragments arranged by size:
3259 915 492 262
SnaBI TAC'GTA
Cuts at: 1612 1612
Size: 4928
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 468 1005 1809 1981 2481 468
Size: 537 804 172 500 2915
Fragments arranged by size:
2915 804 537 500 172
SspI AAT'ATT
Cuts at: 711 711
Size: 4928
StyI C'CwwG_G
Cuts at: 740 2062 2416 740
Size: 1322 354 3252
Fragments arranged by size:
3252 1322 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2511 2644 3103 3312 2511
Size: 133 459 209 4127
Fragments arranged by size:
4127 459 209 133
TatI w'GTAC_w
Cuts at: 449 1296 4443 449
Size: 847 3147 934
Fragments arranged by size:
3147 934 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4445 4477 4445
Size: 32 4896
Tth111I GACn'n_nGTC
Cuts at: 2725 2725
Size: 4928
XbaI T'CTAG_A
Cuts at: 1959 1959
Size: 4928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2423 114
Size: 2309 2619
XmaI C'CCGG_G
Cuts at: 1948 1948
Size: 4928
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PmlI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PpuMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XhoI
XmnI ZraI