(Circular) (Six-base) MAPSORT of: pF1KB7832.seq Check: 3974 from: 1 to: 4943 pF1KB7832 4943 bp With 182 enzymes: SgfI * April 24, 2009 13:08 .. AccI GT'mk_AC Cuts at: 1981 1981 Size: 4943 Acc65I G'GTAC_C Cuts at: 1959 1959 Size: 4943 AclI AA'CG_TT Cuts at: 4783 4783 Size: 4943 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2768 3200 3947 4215 2768 Size: 432 747 268 3496 Fragments arranged by size: 3496 747 432 268 AflIII A'CryG_T Cuts at: 1024 2295 3399 4376 1024 Size: 1271 1104 977 1591 Fragments arranged by size: 1591 1271 1104 977 AgeI A'CCGG_T Cuts at: 3331 3331 Size: 4943 AleI CACnn'nnGTG Cuts at: 125 1109 125 Size: 984 3959 AlwNI CAG_nnn'CTG Cuts at: 333 2248 3815 333 Size: 1915 1567 1461 Fragments arranged by size: 1915 1567 1461 ApaLI G'TGCA_C Cuts at: 859 3713 859 Size: 2854 2089 ApoI r'AATT_y Cuts at: 202 389 1623 1644 1947 4296 4552 202 Size: 187 1234 21 303 2349 256 593 Fragments arranged by size: 2349 1234 593 303 256 187 21 AseI AT'TA_AT Cuts at: 20 20 Size: 4943 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4943 AvaI C'yCGr_G Cuts at: 811 1963 811 Size: 1152 3791 BamHI G'GATC_C Cuts at: 1968 1968 Size: 4943 BanI G'GyrC_C Cuts at: 1959 2373 2621 2656 1959 Size: 414 248 35 4246 Fragments arranged by size: 4246 414 248 35 BanII G_rGCy'C Cuts at: 1565 1957 2987 1565 Size: 392 1030 3521 Fragments arranged by size: 3521 1030 392 BbeI G_GCGC'C Cuts at: 2377 2625 2377 Size: 248 4695 BbsI GAAGACnn'nnnn_ Cuts at: 529 1221 529 Size: 692 4251 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 90 124 90 Size: 34 4909 BciVI GTATCCnnnnn_n' Cuts at: 1842 2841 3602 4943 1842 Size: 999 761 1341 1842 Fragments arranged by size: 1842 1341 999 761 BclI T'GATC_A Cuts at: 297 2463 297 Size: 2166 2777 BfrBI ATG'CAT Cuts at: 727 1245 727 Size: 518 4425 BglII A'GATC_T Cuts at: 2458 4495 2458 Size: 2037 2906 BlpI GC'TnA_GC Cuts at: 2055 2055 Size: 4943 Bme1580I G_kGCm'C Cuts at: 863 2568 2661 3717 863 Size: 1705 93 1056 2089 Fragments arranged by size: 2089 1705 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 701 2155 2236 2571 4715 701 Size: 1454 81 335 2144 929 Fragments arranged by size: 2144 1454 929 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1397 3310 1397 Size: 1913 3030 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1442 1683 1442 Size: 241 4702 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1297 1367 2119 2998 3490 3788 1297 Size: 70 752 879 492 298 2452 Fragments arranged by size: 2452 879 752 492 298 70 BsaI GGTCTCn'nnnn_ Cuts at: 4647 4647 Size: 4943 BsaAI yAC'GTr Cuts at: 2296 2296 Size: 4943 BsaBI GATnn'nnATC Cuts at: 6 2457 4494 6 Size: 2451 2037 455 Fragments arranged by size: 2451 2037 455 BsaHI Gr'CG_yC Cuts at: 2374 2622 3324 4820 2374 Size: 248 702 1496 2497 Fragments arranged by size: 2497 1496 702 248 BsaWI w'CCGG_w Cuts at: 2132 2653 3331 3605 3752 4163 2132 Size: 521 678 274 147 411 2912 Fragments arranged by size: 2912 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1842 1872 1842 Size: 30 4913 BseYI C'CCAG_C Cuts at: 2368 3126 3703 4199 2368 Size: 758 577 496 3112 Fragments arranged by size: 3112 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1700 1724 1700 Size: 24 4919 BsiEI CG_ry'CG Cuts at: 104 2485 2531 3739 104 Size: 2381 46 1208 1308 Fragments arranged by size: 2381 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 134 863 912 1565 1957 2735 2925 3717 134 Size: 729 49 653 392 778 190 792 1360 Fragments arranged by size: 1360 792 778 729 653 392 190 49 BsmI GAATG_Cn' Cuts at: 729 729 Size: 4943 Bsp1286I G_dGCh'C Cuts at: 134 863 912 1565 1957 2568 2661 2735 2925 Size: 729 49 653 392 611 93 74 190 Cuts at: 2925 2987 3717 134 Size: 62 730 1360 Fragments arranged by size: 1360 730 729 653 611 392 190 93 74 62 49 BspEI T'CCGG_A Cuts at: 4163 4163 Size: 4943 BspHI T'CATG_A Cuts at: 4119 4119 Size: 4943 BspMI ACCTGCnnnn'nnnn_ Cuts at: 157 1993 2207 2509 2890 157 Size: 1836 214 302 381 2210 Fragments arranged by size: 2210 1836 381 302 214 BsrBI CCG'CTC Cuts at: 50 3235 3289 4773 4938 50 Size: 3185 54 1484 165 55 Fragments arranged by size: 3185 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2855 2855 Size: 4943 BsrFI r'CCGG_y Cuts at: 2941 3122 3331 2941 Size: 181 209 4553 Fragments arranged by size: 4553 209 181 BssSI C'ACGA_G Cuts at: 3214 3572 3214 Size: 358 4585 BstAPI GCAn_nnn'nTGC Cuts at: 2320 2320 Size: 4943 BstBI TT'CG_AA Cuts at: 3304 3304 Size: 4943 BstXI CCAn_nnnn'nTGG Cuts at: 1111 1111 Size: 4943 BstYI r'GATC_y Cuts at: 1 1968 2435 2458 2793 3039 4051 4137 4149 Size: 1967 467 23 335 246 1012 86 12 Cuts at: 4149 4495 1 Size: 346 449 Fragments arranged by size: 1967 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1554 1554 Size: 4943 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2893 3074 3237 2893 Size: 181 163 4599 Fragments arranged by size: 4599 181 163 BtsI GCAGTG_nn' Cuts at: 2186 2186 Size: 4943 DraI TTT'AAA Cuts at: 213 362 1649 1942 213 Size: 149 1287 293 3214 Fragments arranged by size: 3214 1287 293 149 DrdI GACnn_nn'nnGTC Cuts at: 2649 3507 2649 Size: 858 4085 EaeI y'GGCC_r Cuts at: 2528 2702 3093 3120 4465 2528 Size: 174 391 27 1345 3006 Fragments arranged by size: 3006 1345 391 174 27 EagI C'GGCC_G Cuts at: 2528 2528 Size: 4943 EarI CTCTTCn'nnn_ Cuts at: 416 974 1808 2966 3176 416 Size: 558 834 1158 210 2183 Fragments arranged by size: 2183 1158 834 558 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 979 3151 3461 3607 4753 979 Size: 2172 310 146 1146 1169 Fragments arranged by size: 2172 1169 1146 310 146 EcoICRI GAG'CTC Cuts at: 1563 1955 1563 Size: 392 4551 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1442 1683 2768 3200 3947 4215 1442 Size: 241 1085 432 747 268 2170 Fragments arranged by size: 2170 1085 747 432 268 241 EcoNI CCTnn'n_nnAGG Cuts at: 611 611 Size: 4943 EcoO109I rG'GnC_Cy Cuts at: 2082 2082 Size: 4943 EcoRI G'AATT_C Cuts at: 202 1947 202 Size: 1745 3198 EcoRV GAT'ATC Cuts at: 494 494 Size: 4943 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2962 2962 Size: 4943 FspI TGC'GCA Cuts at: 2724 2724 Size: 4943 HaeII r_GCGC'y Cuts at: 2377 2625 3647 4626 2377 Size: 248 1022 979 2694 Fragments arranged by size: 2694 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 712 744 805 837 1589 1621 1840 1872 2785 Size: 32 61 32 752 32 219 32 913 Cuts at: 2785 2815 2817 2847 712 Size: 30 2 30 2808 Fragments arranged by size: 2808 913 752 219 61 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 818 1886 1982 818 Size: 1068 96 3779 Fragments arranged by size: 3779 1068 96 KasI G'GCGC_C Cuts at: 2373 2621 2373 Size: 248 4695 KpnI G_GTAC'C Cuts at: 1963 1963 Size: 4943 MluI A'CGCG_T Cuts at: 4376 4376 Size: 4943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 317 355 3614 3798 317 Size: 38 3259 184 1462 Fragments arranged by size: 3259 1462 184 38 MscI TGG'CCA Cuts at: 2704 2704 Size: 4943 MslI CAynn'nnrTG Cuts at: 125 224 1109 1166 1182 1250 1505 1521 1676 Size: 99 885 57 16 68 255 16 155 Cuts at: 1676 2932 3059 125 Size: 1256 127 2009 Fragments arranged by size: 2009 1256 885 255 155 127 99 68 57 16 16 MspA1I CmG'CkG Cuts at: 159 1140 2054 2368 2728 3741 3986 159 Size: 981 914 314 360 1013 245 1116 Fragments arranged by size: 1116 1013 981 914 360 314 245 NaeI GCC'GGC Cuts at: 3124 3124 Size: 4943 NarI GG'CG_CC Cuts at: 2374 2622 2374 Size: 248 4695 NdeI CA'TA_TG Cuts at: 1350 1522 1350 Size: 172 4771 NgoMIV G'CCGG_C Cuts at: 3122 3122 Size: 4943 NsiI A_TGCA'T Cuts at: 729 1247 729 Size: 518 4425 NspI r_CATG'y Cuts at: 1028 1153 1245 1833 1996 2496 3403 1028 Size: 125 92 588 163 500 907 2568 Fragments arranged by size: 2568 907 588 500 163 125 92 PciI A'CATG_T Cuts at: 1024 3399 1024 Size: 2375 2568 PmeI GTTT'AAAC Cuts at: 1942 1942 Size: 4943 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4082 4114 4082 Size: 32 4911 PsiI TTA'TAA Cuts at: 557 557 Size: 4943 PspXI vC'TCGA_Gb Cuts at: 811 811 Size: 4943 PstI C_TGCA'G Cuts at: 1990 2675 1990 Size: 685 4258 PvuI CG_AT'CG Cuts at: 104 104 Size: 4943 PvuII CAG'CTG Cuts at: 1140 2368 2728 1140 Size: 1228 360 3355 Fragments arranged by size: 3355 1228 360 RsrII CG'GwC_CG Cuts at: 3138 3138 Size: 4943 SacI G_AGCT'C Cuts at: 1565 1957 1565 Size: 392 4551 SalI G'TCGA_C Cuts at: 1980 1980 Size: 4943 SapI GCTCTTCn'nnn_ Cuts at: 974 1808 2966 3176 974 Size: 834 1158 210 2741 Fragments arranged by size: 2741 1158 834 210 SbfI CC_TGCA'GG Cuts at: 1990 1990 Size: 4943 ScaI AGT'ACT Cuts at: 4460 4460 Size: 4943 SfcI C'TryA_G Cuts at: 33 264 485 584 770 1986 2671 3664 3855 Size: 231 221 99 186 1216 685 993 191 Cuts at: 3855 33 Size: 1121 Fragments arranged by size: 1216 1121 993 685 231 221 191 186 99 SfoI GGC'GCC Cuts at: 2375 2623 2375 Size: 248 4695 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4943 SmaI CCC'GGG Cuts at: 1965 1965 Size: 4943 SmlI C'TyrA_G Cuts at: 811 1312 1382 2098 3013 3505 3767 811 Size: 501 70 716 915 492 262 1987 Fragments arranged by size: 1987 915 716 501 492 262 70 SpeI A'CTAG_T Cuts at: 67 67 Size: 4943 SphI G_CATG'C Cuts at: 1245 1996 2496 1245 Size: 751 500 3692 Fragments arranged by size: 3692 751 500 StyI C'CwwG_G Cuts at: 1001 2077 2431 1001 Size: 1076 354 3513 Fragments arranged by size: 3513 1076 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1568 2526 2659 3118 3327 1568 Size: 958 133 459 209 3184 Fragments arranged by size: 3184 958 459 209 133 TatI w'GTAC_w Cuts at: 4458 4458 Size: 4943 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1320 1352 1564 1596 4460 4492 1320 Size: 32 212 32 2864 32 1771 Fragments arranged by size: 2864 1771 212 32 32 32 Tth111I GACn'n_nGTC Cuts at: 2740 2740 Size: 4943 XbaI T'CTAG_A Cuts at: 1692 1974 1692 Size: 282 4661 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2438 2438 Size: 4943 XhoI C'TCGA_G Cuts at: 811 811 Size: 4943 XmaI C'CCGG_G Cuts at: 1963 1963 Size: 4943 XmnI GAAnn'nnTTC Cuts at: 273 661 273 Size: 388 4555 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PciI PmeI PpiI PsiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI NcoI NheI NotI NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI