Restriction map for
pF1KB7834
(Circular) (Six-base) MAPSORT of: pF1KB7834.seq Check: 2495 from: 1 to: 4856
pF1KB7834 4856 bp
With 182 enzymes: SgfI *
February 11, 2011 12:27 ..
AatII G_ACGT'C
Cuts at: 1110 1110
Size: 4856
AccI GT'mk_AC
Cuts at: 1894 1894
Size: 4856
Acc65I G'GTAC_C
Cuts at: 1872 1872
Size: 4856
AclI AA'CG_TT
Cuts at: 4696 4696
Size: 4856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 953 1106 1687 2681 3113 3860 4128 604
Size: 349 153 581 994 432 747 268 1332
Fragments arranged by size:
1332 994 747 581 432 349 268 153
AflIII A'CryG_T
Cuts at: 575 2208 3312 4289 575
Size: 1633 1104 977 1142
Fragments arranged by size:
1633 1142 1104 977
AgeI A'CCGG_T
Cuts at: 3244 3244
Size: 4856
AhdI GACnn_n'nnGTC
Cuts at: 344 344
Size: 4856
AlwNI CAG_nnn'CTG
Cuts at: 418 1583 1755 2161 3728 418
Size: 1165 172 406 1567 1546
Fragments arranged by size:
1567 1546 1165 406 172
ApaLI G'TGCA_C
Cuts at: 3626 3626
Size: 4856
ApoI r'AATT_y
Cuts at: 831 1123 1410 1860 4209 4465 831
Size: 292 287 450 2349 256 1222
Fragments arranged by size:
2349 1222 450 292 287 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4856
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
AvaI C'yCGr_G
Cuts at: 178 227 962 1876 178
Size: 49 735 914 3158
Fragments arranged by size:
3158 914 735 49
BamHI G'GATC_C
Cuts at: 1812 1881 1812
Size: 69 4787
BanI G'GyrC_C
Cuts at: 771 1872 2286 2534 2569 771
Size: 1101 414 248 35 3058
Fragments arranged by size:
3058 1101 414 248 35
BanII G_rGCy'C
Cuts at: 1337 1870 2900 1337
Size: 533 1030 3293
Fragments arranged by size:
3293 1030 533
BbeI G_GCGC'C
Cuts at: 2290 2538 2290
Size: 248 4608
BbvCI CC'TCA_GC
Cuts at: 243 243
Size: 4856
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4822
BciVI GTATCCnnnnn_n'
Cuts at: 1450 2754 3515 4856 1450
Size: 1304 761 1341 1450
Fragments arranged by size:
1450 1341 1304 761
BclI T'GATC_A
Cuts at: 465 2376 465
Size: 1911 2945
BglI GCCn_nnn'nGGC
Cuts at: 1780 1780
Size: 4856
BglII A'GATC_T
Cuts at: 174 1262 1554 2371 4408 174
Size: 1088 292 817 2037 622
Fragments arranged by size:
2037 1088 817 622 292
BlpI GC'TnA_GC
Cuts at: 253 1968 253
Size: 1715 3141
Bme1580I G_kGCm'C
Cuts at: 1849 2481 2574 3630 1849
Size: 632 93 1056 3075
Fragments arranged by size:
3075 1056 632 93
BmrI ACTGGGnnnn_n'
Cuts at: 358 2068 2149 2484 4628 358
Size: 1710 81 335 2144 586
Fragments arranged by size:
2144 1710 586 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3223 3223
Size: 4856
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 519
Size: 4856
Bpu10I CC'TnA_GC
Cuts at: 243 243
Size: 4856
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 2032 2911 3403 3701 251
Size: 1781 879 492 298 1406
Fragments arranged by size:
1781 1406 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4560 4560
Size: 4856
BsaAI yAC'GTr
Cuts at: 2209 2209
Size: 4856
BsaBI GATnn'nnATC
Cuts at: 6 1636 2370 4407 6
Size: 1630 734 2037 455
Fragments arranged by size:
2037 1630 734 455
BsaHI Gr'CG_yC
Cuts at: 1107 2287 2535 3237 4733 1107
Size: 1180 248 702 1496 1230
Fragments arranged by size:
1496 1230 1180 702 248
BsaWI w'CCGG_w
Cuts at: 2045 2566 3244 3518 3665 4076 2045
Size: 521 678 274 147 411 2825
Fragments arranged by size:
2825 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1417 1447 1417
Size: 30 4826
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 231 325 231
Size: 94 4762
BseYI C'CCAG_C
Cuts at: 1101 1816 2281 3039 3616 4112 1101
Size: 715 465 758 577 496 1845
Fragments arranged by size:
1845 758 715 577 496 465
BsiEI CG_ry'CG
Cuts at: 104 2398 2444 3652 104
Size: 2294 46 1208 1308
Fragments arranged by size:
2294 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 185 957 1870 2648 2838 3630 185
Size: 772 913 778 190 792 1411
Fragments arranged by size:
1411 913 792 778 772 190
BsmI GAATG_Cn'
Cuts at: 132 769 132
Size: 637 4219
BsmBI CGTCTCn'nnnn_
Cuts at: 1114 1437 1651 1114
Size: 323 214 4319
Fragments arranged by size:
4319 323 214
Bsp1286I G_dGCh'C
Cuts at: 185 957 1337 1849 1870 2481 2574 2648 2838
Size: 772 380 512 21 611 93 74 190
Cuts at: 2838 2900 3630 185
Size: 62 730 1411
Fragments arranged by size:
1411 772 730 611 512 380 190 93
74 62 21
BspEI T'CCGG_A
Cuts at: 4076 4076
Size: 4856
BspHI T'CATG_A
Cuts at: 468 4032 468
Size: 3564 1292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1616 1906 2120 2422 2803 1616
Size: 290 214 302 381 3669
Fragments arranged by size:
3669 381 302 290 214
BsrBI CCG'CTC
Cuts at: 50 239 3148 3202 4686 4851 50
Size: 189 2909 54 1484 165 55
Fragments arranged by size:
2909 1484 189 165 55 54
BsrDI GCAATG_nn'
Cuts at: 563 2768 563
Size: 2205 2651
BsrFI r'CCGG_y
Cuts at: 1163 2854 3035 3244 1163
Size: 1691 181 209 2775
Fragments arranged by size:
2775 1691 209 181
BssSI C'ACGA_G
Cuts at: 3127 3485 3127
Size: 358 4498
BstAPI GCAn_nnn'nTGC
Cuts at: 2233 2233
Size: 4856
BstBI TT'CG_AA
Cuts at: 285 3217 285
Size: 2932 1924
BstYI r'GATC_y
Cuts at: 1 174 939 1262 1554 1812 1881 2348 2371
Size: 173 765 323 292 258 69 467 23
Cuts at: 2371 2706 2952 3964 4050 4062 4408 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 765 467 449 346 335 323 292
258 246 173 86 69 23 12
BtgI C'CryG_G
Cuts at: 1808 1808
Size: 4856
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 595 2806 2987 3150 595
Size: 2211 181 163 2301
Fragments arranged by size:
2301 2211 181 163
BtsI GCAGTG_nn'
Cuts at: 603 2099 603
Size: 1496 3360
DraI TTT'AAA
Cuts at: 1133 1742 1855 1133
Size: 609 113 4134
Fragments arranged by size:
4134 609 113
DraIII CAC_nnn'GTG
Cuts at: 1201 1201
Size: 4856
DrdI GACnn_nn'nnGTC
Cuts at: 2562 3420 2562
Size: 858 3998
EaeI y'GGCC_r
Cuts at: 1044 1172 1289 2441 2615 3006 3033 4378 1044
Size: 128 117 1152 174 391 27 1345 1522
Fragments arranged by size:
1522 1345 1152 391 174 128 117 27
EagI C'GGCC_G
Cuts at: 2441 2441
Size: 4856
EarI CTCTTCn'nnn_
Cuts at: 115 327 392 945 1138 2879 3089 115
Size: 212 65 553 193 1741 210 1882
Fragments arranged by size:
1882 1741 553 212 210 193 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1150 1378 3064 3374 3520 4666 1150
Size: 228 1686 310 146 1146 1340
Fragments arranged by size:
1686 1340 1146 310 228 146
EcoICRI GAG'CTC
Cuts at: 1868 1868
Size: 4856
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 519 604 953 1106 1687 2681 3113 3860 4128
Size: 85 349 153 581 994 432 747 268
Cuts at: 4128 519
Size: 1247
Fragments arranged by size:
1247 994 747 581 432 349 268 153
85
EcoNI CCTnn'n_nnAGG
Cuts at: 246 668 246
Size: 422 4434
EcoO109I rG'GnC_Cy
Cuts at: 1752 1995 1752
Size: 243 4613
EcoRI G'AATT_C
Cuts at: 1123 1410 1860 1123
Size: 287 450 4119
Fragments arranged by size:
4119 450 287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1612 2875 1612
Size: 1263 3593
FspI TGC'GCA
Cuts at: 2637 2637
Size: 4856
HaeII r_GCGC'y
Cuts at: 624 2290 2538 3560 4539 624
Size: 1666 248 1022 979 941
Fragments arranged by size:
1666 1022 979 941 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 196 228 330 362 2698 2728 2730 2760 196
Size: 32 102 32 2336 30 2 30 2292
Fragments arranged by size:
2336 2292 102 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1895 1895
Size: 4856
KasI G'GCGC_C
Cuts at: 2286 2534 2286
Size: 248 4608
KpnI G_GTAC'C
Cuts at: 1876 1876
Size: 4856
MfeI C'AATT_G
Cuts at: 1034 1034
Size: 4856
MluI A'CGCG_T
Cuts at: 4289 4289
Size: 4856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 517 1817 3527 3711 517
Size: 1300 1710 184 1662
Fragments arranged by size:
1710 1662 1300 184
MscI TGG'CCA
Cuts at: 1174 1291 2617 1174
Size: 117 1326 3413
Fragments arranged by size:
3413 1326 117
MslI CAynn'nnrTG
Cuts at: 2845 2972 2845
Size: 127 4729
MspA1I CmG'CkG
Cuts at: 463 757 1820 1967 2281 2641 3654 3899 463
Size: 294 1063 147 314 360 1013 245 1420
Fragments arranged by size:
1420 1063 1013 360 314 294 245 147
NaeI GCC'GGC
Cuts at: 1165 3037 1165
Size: 1872 2984
NarI GG'CG_CC
Cuts at: 2287 2535 2287
Size: 248 4608
NcoI C'CATG_G
Cuts at: 1808 1808
Size: 4856
NgoMIV G'CCGG_C
Cuts at: 1163 3035 1163
Size: 1872 2984
NspI r_CATG'y
Cuts at: 579 1909 2409 3316 579
Size: 1330 500 907 2119
Fragments arranged by size:
2119 1330 907 500
PciI A'CATG_T
Cuts at: 575 3312 575
Size: 2737 2119
PmeI GTTT'AAAC
Cuts at: 1855 1855
Size: 4856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1532 1564 3995 4027 1532
Size: 32 2431 32 2361
Fragments arranged by size:
2431 2361 32 32
PpuMI rG'GwC_Cy
Cuts at: 1752 1752
Size: 4856
PsiI TTA'TAA
Cuts at: 1567 1567
Size: 4856
PspXI vC'TCGA_Gb
Cuts at: 227 227
Size: 4856
PstI C_TGCA'G
Cuts at: 296 1903 2588 296
Size: 1607 685 2564
Fragments arranged by size:
2564 1607 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4856
PvuII CAG'CTG
Cuts at: 463 757 1820 2281 2641 463
Size: 294 1063 461 360 2678
Fragments arranged by size:
2678 1063 461 360 294
RsrII CG'GwC_CG
Cuts at: 3051 3051
Size: 4856
SacI G_AGCT'C
Cuts at: 1870 1870
Size: 4856
SalI G'TCGA_C
Cuts at: 1893 1893
Size: 4856
SapI GCTCTTCn'nnn_
Cuts at: 945 1138 2879 3089 945
Size: 193 1741 210 2712
Fragments arranged by size:
2712 1741 210 193
SbfI CC_TGCA'GG
Cuts at: 1903 1903
Size: 4856
ScaI AGT'ACT
Cuts at: 1595 4373 1595
Size: 2778 2078
SexAI A'CCwGG_T
Cuts at: 1063 1197 1063
Size: 134 4722
SfcI C'TryA_G
Cuts at: 33 170 292 1899 2584 3577 3768 33
Size: 137 122 1607 685 993 191 1121
Fragments arranged by size:
1607 1121 993 685 191 137 122
SfoI GGC'GCC
Cuts at: 2288 2536 2288
Size: 248 4608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4856
SmaI CCC'GGG
Cuts at: 964 1878 964
Size: 914 3942
SmlI C'TyrA_G
Cuts at: 178 227 266 2011 2926 3418 3680 178
Size: 49 39 1745 915 492 262 1354
Fragments arranged by size:
1745 1354 915 492 262 49 39
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4856
SphI G_CATG'C
Cuts at: 1909 2409 1909
Size: 500 4356
SrfI GCCC'GGGC
Cuts at: 964 964
Size: 4856
StyI C'CwwG_G
Cuts at: 569 1808 1841 1990 2344 569
Size: 1239 33 149 354 3081
Fragments arranged by size:
3081 1239 354 149 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1208 2439 2572 3031 3240 1208
Size: 1231 133 459 209 2824
Fragments arranged by size:
2824 1231 459 209 133
TatI w'GTAC_w
Cuts at: 1457 1593 4371 1457
Size: 136 2778 1942
Fragments arranged by size:
2778 1942 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4373 4405 4373
Size: 32 4824
Tth111I GACn'n_nGTC
Cuts at: 1681 2653 1681
Size: 972 3884
XbaI T'CTAG_A
Cuts at: 1887 1887
Size: 4856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1299 1806 2351 1299
Size: 507 545 3804
Fragments arranged by size:
3804 545 507
XhoI C'TCGA_G
Cuts at: 178 227 178
Size: 49 4807
XmaI C'CCGG_G
Cuts at: 962 1876 962
Size: 914 3942
XmnI GAAnn'nnTTC
Cuts at: 1414 1595 1414
Size: 181 4675
ZraI GAC'GTC
Cuts at: 1108 1108
Size: 4856
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PpuMI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BsgI BsiWI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PshAI
PspOMI PsrI SacII SanDI SfiI SgrAI SnaBI SspI
StuI SwaI